Research Article
The Analysis of Water Quality Index with Study of the Effect of Textile Effluents on the Groundwater of Bhilwara City
Issue:
Volume 13, Issue 1, March 2025
Pages:
1-8
Received:
10 February 2025
Accepted:
24 February 2025
Published:
18 March 2025
Abstract: Contamination of water is a big concern that is now existing not just in India but also all around the world. In the city of Bhilwara, which is located in India, there is a lack of well-organized drainage and sewer infrastructure, as well as poor management of solid waste. The quality of the water is decreasing as a result of the discharge of these contaminants into the Banas River, which is located nearby, through a variety of different channels. Therefore, the purpose of this study is to evaluate the existing groundwater quality in Bhilwara city by utilizing hadrochemical, multivariate statistical, and Water Quality Index (WQI) status. According to the conclusions of the study, the amount of potable water that is available in this little community is gradually decreasing. The water quality index (WQI) of the source, which can range anywhere from 62 to 74, reveals that the quality of the groundwater is deteriorating on a daily basis. There should be a comprehensive management strategy that includes monitoring cells, according to the findings of this study, in order to protect the water environment in the Bhilwara region.
Abstract: Contamination of water is a big concern that is now existing not just in India but also all around the world. In the city of Bhilwara, which is located in India, there is a lack of well-organized drainage and sewer infrastructure, as well as poor management of solid waste. The quality of the water is decreasing as a result of the discharge of these...
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Research Article
The Construction of Liquid Crystal / Fluorescence Dual-Signal Immunosensor Based on DNA-Antibody Conjugate
Lijuan Zhu
,
Qiongzheng Hu,
Ru-Song Zhao*
Issue:
Volume 13, Issue 1, March 2025
Pages:
9-13
Received:
25 March 2025
Accepted:
30 April 2025
Published:
22 May 2025
DOI:
10.11648/j.mc.20251301.12
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Abstract: A liquid crystal / fluorescence dual-signal immunosensor is developed for the sensitive detection of aflatoxin B1 (AFB1). Through click chemistry reaction, DNA probe is covalently conjugated to the antibody, constructing a detection system with liquid crystal (LC)/fluorescence dual-signal output capabilities. After co-incubation of DNA-antibody (Ab) conjugate, active DNA, and AFB1, the mixture is transferred to 96-well plates that have been pre-functionalized with AFB1 antigen for competitive binding. In the presence of AFB1, the separated supernatant can induce alignment changes in LC molecules, while the DNA-Ab complexes captured on the surface of 96-well plates can activate CRISPR/Cas12a to cleavage the ssDNA reporter, generating fluorescent signals. Notably, the active DNA demonstrated dual functionality. It can not only hybridize with DNA-antibody conjugate but also activate the CRISPR/Cas12a to cleavage the ssDNA reporter, generating fluorescent signals. As AFB1 concentrations increase, the reduced capture of DNA-Ab complexes on the 96-well plates leads to an increase in DNA and DNA-antibody complexes in the supernatant. It causes the LC image change from dark to bright, while the decreased capture of activator DNA results in the decrease of fluorescence signals. Overall, this dual-signal sensor exhibits high specificity, stability, and reproducibility, with simple operation enabling rapid AFB1 detection, it has the potential of application in real samples.
Abstract: A liquid crystal / fluorescence dual-signal immunosensor is developed for the sensitive detection of aflatoxin B1 (AFB1). Through click chemistry reaction, DNA probe is covalently conjugated to the antibody, constructing a detection system with liquid crystal (LC)/fluorescence dual-signal output capabilities. After co-incubation of DNA-antibody (Ab...
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