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Determination of the Antibacterial and Antioxidant Activity of Crude Extract of Bruceaantidysenterica Leaves

Received: 1 February 2023     Accepted: 14 March 2023     Published: 31 March 2023
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Abstract

Bruceaantidysenterica (abalo in Amharic) is a medicinal plant widely used in traditional medicine for treatment of several diseases and ailments. This study aimed to investigate the classes of the phytochemical constituents of the leaves of Bruceaantidysenterica extracted with the solvents of petroleum ether, ethyl acetate, and methanol. The crud extract evaluated their antioxidant and antimicrobial activities. Qualitative phytochemical screening of the leaves of this plant indicated the presence of alkaloids, flavonoids, phenols, quinones, steroids, terpenoids, saponins, tannins and cumaroins. Quantitative analysis of the leaves of Bruceaantidysenterica revealed that the total phenolic content, expressed as gallic acid equivalent, ranged from 28.5 to 120.06 mg/g of dry weight of extracts. Likewise, the total flavonoid content of the extract, expressed as quercetin equivalent, varied from 26.05 to 83.663 mg/g of dry weight of extracts. All extracts showed antioxidant and antimicrobial activity. As seen from ferric reducing antioxidant power (FRAP) assay, methanol and ethyl acetate extracts of the leaves of Bruceaantidysenterica showed the highest total reducing power while petroleum ether extract exhibited the lowest total reducing power. Furthermore, all the extracts were tested against two gram positive bacteria (Staphylococcus aureus, Streptococcus pyogene) and two gram negative bacteria (Escherichia coli, k. pneumonia) bacteria by Kirby-Bauer standard disc diffusion method. Antibacterial effects of leaves extracts of bruceaantidysenterica showed different degrees of inhibition against both Gram positive and Gram negative bacteria. The methanol and ethyl acetate extracts showed higher antibacterial activity than petroleum ether extracts.

Published in Modern Chemistry (Volume 11, Issue 1)
DOI 10.11648/j.mc.20231101.13
Page(s) 34-42
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Bruceaantidysenterica, Antioxidant Activity, Phytochemical Constituent, Antibacterial Activity

References
[1] Koduru, S., Grierson, D. S. and Afolayan, A. J., 2006. Antimicrobial Activity of Solanumaculeastrum. Pharmaceutical biology, 44 (4), pp. 283-286.
[2] Saiprasanna, B., Manohar, B., Roja, R., Prasanta K., Rajeshree, P. (2012). Phytochemical Investigation and Study on Antioxidant Properties of Ocimumcanum Hydro Alcoholic Leaf Extracts, Journal of Drug Delivery and Therapeutics, 2, pp. 122-128.
[3] DawitAbebe. (2001). The role of medicinal plants in healthcare coverage of Ethiopia, the possible benefits of integration. In: Proceedings of Workshop on Biodiversity Conservation and Sustainable Use of Medicinal Plants in Ethiopia. Addis Ababa, Ethiopia.
[4] Hamilton, A. (2001). Medicinal Plants and Conservation: issues and approaches. The United Kingdom.
[5] Kong, J. M., Goh, N. K., Chia, L. S. and Chia, T. F., 2003. Recent advances in traditional plant drugs and orchids. ActaPharmacologicaSinica, 24 (1), pp. 7-21.
[6] Endashaw B. (2007). Study on Actual Situation of Medicinal Plants in Ethiopia. Available from: http://www.endashaw.com.
[7] Paul, A., Bharali, S., Khan, M. L. and Tripathi, O. P., 2013. Anthropogenic disturbances led to the risk of extinction of TaxuswallichianaZuccarini, an endangered medicinal tree in Arunachal Himalaya. Natural areas journal, 33 (4), pp. 447-454.
[8] Tesfaye, A., Study of the plants in Gambella region, MSc Thesis Addis Ababa University, Ethiopia. 2003.
[9] Ahvazi, M., Khalighi-Sigaroodi, F., Charkhchiyan, M. M., Mojab, F., Mozaffarian, V. A. and Zakeri, H., 2012. Introduction of medicinal plant species with the most traditional usage in the Alamut region. Iranian Journal of pharmaceutical research: IJPR, 11 (1), pp. 185.
[10] Ugochukwu, S., Uche, I., and Ifeanyi, O. (2013). Preliminary phytochemical screening of different solvent extracts of stem, bark, and roots of DennetiaTripetala G. Baker. Asian Journal of Plant Science and Research, 3, pp. 10-13.
[11] Kumar, V. G., Gokavarapu, S. D., Rajeswari, A., Dhas, T. S., Karthick, V., Kapadia, Z., Shrestha, T., Barathy, I. A., Roy, A. and Sinha, S., 2011. Facile green synthesis of gold nanoparticles using leaf extract of antidiabetic potent Cassia auriculata. Colloids and Surfaces B: Biointerfaces, 87 (1), pp. 159-163.
[12] Giese, J., 1996. Antioxidants: tools for preventing lipid oxidation. Food technology (Chicago), 50 (11), pp. 73-81.
[13] Arihan, O., 2007. Traditional medicine and intellectual property rights. Ankara ÜniversitesiEczacılıkFakültesiDergisi, 36 (2), pp. 135-152.
[14] Parajuli, S., Pun, N. T., Parajuli, S. and Jamarkattel-Pandit, N., 2012. Antioxidant Activity, Total Phenol and Flavanoid Contents in Some Selected Medicinal Plants of Nepal. Journal of Health and Allied Sciences, 2 (1), pp. 27-31.
[15] Guleria, S., Tiku, A. K., Singh, G., Koul, A., Gupta, S. and Rana, S., 2013. In vitro antioxidant activity and phenolic contents in methanol extracts from medicinal plants. Journal of plant biochemistry and biotechnology, 22 (1), pp. 9-15.
[16] Cai, Y., Sun, M. and Corke, H., 2003. Antioxidant activity of betalains from plants of the Amaranthaceae. Journal of agricultural and food chemistry, 51 (8), pp. 2288-2294.
[17] Amic, D., Davidović-Amic, D., Beslo, D. and Trinajstic, N., 2003. Structure-radical scavenging activity relationships of flavonoids. Croatica chemicalActa, 76 (1), pp. 55-61.
[18] Scalbert, A., Manach, C., Morand, C., Rémésy, C. and Jiménez, L., 2005. Dietary polyphenols and the prevention of diseases. Critical reviews in food science and nutrition, 45 (4), pp. 287-306.
[19] Sen, S., Chakraborty, R., Sridhar, C., Reddy, Y. S. R. and De, B., 2010. Free radicals, antioxidants, diseases, and phytomedicines: current status and prospect. International journal of pharmaceutical sciences review and research, 3 (1), pp. 91-100.
[20] Cowan, M. M., 1999. Plant products as antimicrobial agents. Clinical microbiology reviews, 12 (4), pp. 564-582.
[21] Dutta, A. K., Gope, P. S., Makhnoon, S., Rahman, M. S., Siddiquee, M. A., Yearul, K. Y. (2012). Effect of solvent extraction on phenolic content, antioxidant and amylase inhibition activities of Swertiachirata, Int. J. Drug Development & Research, 4 (4), pp. 317-325.
[22] Curcino Vieira, I. J., Braz-Filho, R., (2006). Quassinoids: structural diversity, biological activity, and synthetic studies. Studies production chemical, 33, pp. 433-492.
[23] Clark, E. P., 1937. Quassin. I. The preparation and purification of quassin and neoquassin, with information concerning their molecular formulas. Journal of the American Chemical Society, 59 (5), pp. 927-931.
[24] Dilika, F., Afolayan, A. and Meyer, J. (1996). Comparative antibacterial activity of two Helichrysum species used male circumcision in South Africa. South African Journal of Botany, 63, pp. 158-159.
[25] Green, R. J. (2004). Antioxidant Activity of Peanut Plant Tissues. Master’s Thesis. North Carolina State University. The USA.
[26] Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, JournalScience Technology, 26, pp. 211-219.
[27] Haregewine Tadese July. (2005). Phytochemical Investigation on the Seeds of Bruceaantidysenterica. Addis Ababa University School of Graduate Studies.
[28] Prior, R. L., Wu, X. and Schaich, K., 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of agricultural and food chemistry, 53 (10), pp. 4290-4302.
Cite This Article
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    Liyew Yizengaw Yitayih, Thevabakthi Siluvai Muthu Arul Jeevan, Solomon Libsu Bikilla, Tesfahun Dagnaw Demelash. (2023). Determination of the Antibacterial and Antioxidant Activity of Crude Extract of Bruceaantidysenterica Leaves. Modern Chemistry, 11(1), 34-42. https://doi.org/10.11648/j.mc.20231101.13

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    ACS Style

    Liyew Yizengaw Yitayih; Thevabakthi Siluvai Muthu Arul Jeevan; Solomon Libsu Bikilla; Tesfahun Dagnaw Demelash. Determination of the Antibacterial and Antioxidant Activity of Crude Extract of Bruceaantidysenterica Leaves. Mod. Chem. 2023, 11(1), 34-42. doi: 10.11648/j.mc.20231101.13

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    AMA Style

    Liyew Yizengaw Yitayih, Thevabakthi Siluvai Muthu Arul Jeevan, Solomon Libsu Bikilla, Tesfahun Dagnaw Demelash. Determination of the Antibacterial and Antioxidant Activity of Crude Extract of Bruceaantidysenterica Leaves. Mod Chem. 2023;11(1):34-42. doi: 10.11648/j.mc.20231101.13

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  • @article{10.11648/j.mc.20231101.13,
      author = {Liyew Yizengaw Yitayih and Thevabakthi Siluvai Muthu Arul Jeevan and Solomon Libsu Bikilla and Tesfahun Dagnaw Demelash},
      title = {Determination of the Antibacterial and Antioxidant Activity of Crude Extract of Bruceaantidysenterica Leaves},
      journal = {Modern Chemistry},
      volume = {11},
      number = {1},
      pages = {34-42},
      doi = {10.11648/j.mc.20231101.13},
      url = {https://doi.org/10.11648/j.mc.20231101.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.mc.20231101.13},
      abstract = {Bruceaantidysenterica (abalo in Amharic) is a medicinal plant widely used in traditional medicine for treatment of several diseases and ailments. This study aimed to investigate the classes of the phytochemical constituents of the leaves of Bruceaantidysenterica extracted with the solvents of petroleum ether, ethyl acetate, and methanol. The crud extract evaluated their antioxidant and antimicrobial activities. Qualitative phytochemical screening of the leaves of this plant indicated the presence of alkaloids, flavonoids, phenols, quinones, steroids, terpenoids, saponins, tannins and cumaroins. Quantitative analysis of the leaves of Bruceaantidysenterica revealed that the total phenolic content, expressed as gallic acid equivalent, ranged from 28.5 to 120.06 mg/g of dry weight of extracts. Likewise, the total flavonoid content of the extract, expressed as quercetin equivalent, varied from 26.05 to 83.663 mg/g of dry weight of extracts. All extracts showed antioxidant and antimicrobial activity. As seen from ferric reducing antioxidant power (FRAP) assay, methanol and ethyl acetate extracts of the leaves of Bruceaantidysenterica showed the highest total reducing power while petroleum ether extract exhibited the lowest total reducing power. Furthermore, all the extracts were tested against two gram positive bacteria (Staphylococcus aureus, Streptococcus pyogene) and two gram negative bacteria (Escherichia coli, k. pneumonia) bacteria by Kirby-Bauer standard disc diffusion method. Antibacterial effects of leaves extracts of bruceaantidysenterica showed different degrees of inhibition against both Gram positive and Gram negative bacteria. The methanol and ethyl acetate extracts showed higher antibacterial activity than petroleum ether extracts.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Determination of the Antibacterial and Antioxidant Activity of Crude Extract of Bruceaantidysenterica Leaves
    AU  - Liyew Yizengaw Yitayih
    AU  - Thevabakthi Siluvai Muthu Arul Jeevan
    AU  - Solomon Libsu Bikilla
    AU  - Tesfahun Dagnaw Demelash
    Y1  - 2023/03/31
    PY  - 2023
    N1  - https://doi.org/10.11648/j.mc.20231101.13
    DO  - 10.11648/j.mc.20231101.13
    T2  - Modern Chemistry
    JF  - Modern Chemistry
    JO  - Modern Chemistry
    SP  - 34
    EP  - 42
    PB  - Science Publishing Group
    SN  - 2329-180X
    UR  - https://doi.org/10.11648/j.mc.20231101.13
    AB  - Bruceaantidysenterica (abalo in Amharic) is a medicinal plant widely used in traditional medicine for treatment of several diseases and ailments. This study aimed to investigate the classes of the phytochemical constituents of the leaves of Bruceaantidysenterica extracted with the solvents of petroleum ether, ethyl acetate, and methanol. The crud extract evaluated their antioxidant and antimicrobial activities. Qualitative phytochemical screening of the leaves of this plant indicated the presence of alkaloids, flavonoids, phenols, quinones, steroids, terpenoids, saponins, tannins and cumaroins. Quantitative analysis of the leaves of Bruceaantidysenterica revealed that the total phenolic content, expressed as gallic acid equivalent, ranged from 28.5 to 120.06 mg/g of dry weight of extracts. Likewise, the total flavonoid content of the extract, expressed as quercetin equivalent, varied from 26.05 to 83.663 mg/g of dry weight of extracts. All extracts showed antioxidant and antimicrobial activity. As seen from ferric reducing antioxidant power (FRAP) assay, methanol and ethyl acetate extracts of the leaves of Bruceaantidysenterica showed the highest total reducing power while petroleum ether extract exhibited the lowest total reducing power. Furthermore, all the extracts were tested against two gram positive bacteria (Staphylococcus aureus, Streptococcus pyogene) and two gram negative bacteria (Escherichia coli, k. pneumonia) bacteria by Kirby-Bauer standard disc diffusion method. Antibacterial effects of leaves extracts of bruceaantidysenterica showed different degrees of inhibition against both Gram positive and Gram negative bacteria. The methanol and ethyl acetate extracts showed higher antibacterial activity than petroleum ether extracts.
    VL  - 11
    IS  - 1
    ER  - 

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Author Information
  • Department of Chemistry, College of Natural and Computational Science, MizanTepi University, Mizan, Ethiopia

  • Department of Chemistry, College of Natural and Computational Science, MizanTepi University, Mizan, Ethiopia

  • Department of Chemistry, College of Natural and Computational Science, Bahir Dar University, Bahir Dar, Ethiopia

  • Department of Chemistry, College of Natural and Computational Science, WolayitaSedo University, Wolayita, Ethiopia

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