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Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire

Received: 24 September 2022     Accepted: 28 October 2022     Published: 8 December 2022
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Abstract

Rice is the third most produced cereal in the world after maize and wheat. In Côte d'Ivoire, rice is the main food of the population. However, despite its importance, rice cultivation is subject to biotic constraints, including rice yellow mottle virus (RYMV), which is the most damaging viral disease of rice native to Africa. RYMV is a highly variable virus. This study aims to assess the ability of RYMV isolates to overcome the resistance-breaking genes RYMV1, RYMV2 and RYMV3. Sixteen RYMV isolates from Côte d'Ivoire representative of the agro-ecological zones (AEZ) visited and the varieties Bouake 189, Gigante, Tog 5681, Tog 5674, Tog 7291 and Tog 5307, carrying respectively rymv1-1 rymv1-2, rymv1-3, rymv1-5, RYMV2 and RYMV3 alleles were used were used. Varieties were sown in trays filled with sterilized soil so as to keep six plants per variety, i.e. 36 plants per tray per isolate. Rice plants were inoculated mechanically 14 days after sowing. Leaves of infected plants were collected individually per variety 45 days after inoculation in order to determine the virus concentration by serological DAS-ELISA tests. Asymptomatic infections were also investigated. The results showed that 37.5% of the tested isolates broke only the rymv1-2 gene with the presence of apparent symptoms. However, 12.5% of the isolates are infected both Gigante and Tog5674. None of the 16 isolates tested could induce symptoms in Tog5703, Tog5681 and Tog7291. Furthermore, only Tog7291 are showed the presence of the virus without apparent symptoms on rice leaves after inoculation. The identification of such isolates in rice-growing areas raises a serious threat to Ivorian rice production and calls for surveillance of this disease in Côte d'Ivoire.

Published in Journal of Plant Sciences (Volume 10, Issue 6)
DOI 10.11648/j.jps.20221006.16
Page(s) 235-240
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

RYMV, Resistance-Breaking, Gigante, Tog 5674, Côte d'Ivoire

References
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[20] Pinel-Galzi, A., Dubreuil-Tranchant, C., Hébrard, E., Mariac, C., Ghesquière, A. and Albar L., 2016. Mutations in Rice yellow mottle virus Polyprotein P2a involved in RYMV2 gene resistance breakdown. Frontiers in Plant Science, 7: 1-11.
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    Guinagui N’Doua Bertrand, Yeo Gnenakan, Tuo Seydou, Kouda Affiba Genevieve, Lehi Malidy Irenee, et al. (2022). Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire. Journal of Plant Sciences, 10(6), 235-240. https://doi.org/10.11648/j.jps.20221006.16

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    Guinagui N’Doua Bertrand; Yeo Gnenakan; Tuo Seydou; Kouda Affiba Genevieve; Lehi Malidy Irenee, et al. Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire. J. Plant Sci. 2022, 10(6), 235-240. doi: 10.11648/j.jps.20221006.16

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    AMA Style

    Guinagui N’Doua Bertrand, Yeo Gnenakan, Tuo Seydou, Kouda Affiba Genevieve, Lehi Malidy Irenee, et al. Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire. J Plant Sci. 2022;10(6):235-240. doi: 10.11648/j.jps.20221006.16

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  • @article{10.11648/j.jps.20221006.16,
      author = {Guinagui N’Doua Bertrand and Yeo Gnenakan and Tuo Seydou and Kouda Affiba Genevieve and Lehi Malidy Irenee and Danon Aubin Silvere Djiwha and Fatogoma Sorho},
      title = {Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire},
      journal = {Journal of Plant Sciences},
      volume = {10},
      number = {6},
      pages = {235-240},
      doi = {10.11648/j.jps.20221006.16},
      url = {https://doi.org/10.11648/j.jps.20221006.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20221006.16},
      abstract = {Rice is the third most produced cereal in the world after maize and wheat. In Côte d'Ivoire, rice is the main food of the population. However, despite its importance, rice cultivation is subject to biotic constraints, including rice yellow mottle virus (RYMV), which is the most damaging viral disease of rice native to Africa. RYMV is a highly variable virus. This study aims to assess the ability of RYMV isolates to overcome the resistance-breaking genes RYMV1, RYMV2 and RYMV3. Sixteen RYMV isolates from Côte d'Ivoire representative of the agro-ecological zones (AEZ) visited and the varieties Bouake 189, Gigante, Tog 5681, Tog 5674, Tog 7291 and Tog 5307, carrying respectively rymv1-1 rymv1-2, rymv1-3, rymv1-5, RYMV2 and RYMV3 alleles were used were used. Varieties were sown in trays filled with sterilized soil so as to keep six plants per variety, i.e. 36 plants per tray per isolate. Rice plants were inoculated mechanically 14 days after sowing. Leaves of infected plants were collected individually per variety 45 days after inoculation in order to determine the virus concentration by serological DAS-ELISA tests. Asymptomatic infections were also investigated. The results showed that 37.5% of the tested isolates broke only the rymv1-2 gene with the presence of apparent symptoms. However, 12.5% of the isolates are infected both Gigante and Tog5674. None of the 16 isolates tested could induce symptoms in Tog5703, Tog5681 and Tog7291. Furthermore, only Tog7291 are showed the presence of the virus without apparent symptoms on rice leaves after inoculation. The identification of such isolates in rice-growing areas raises a serious threat to Ivorian rice production and calls for surveillance of this disease in Côte d'Ivoire.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire
    AU  - Guinagui N’Doua Bertrand
    AU  - Yeo Gnenakan
    AU  - Tuo Seydou
    AU  - Kouda Affiba Genevieve
    AU  - Lehi Malidy Irenee
    AU  - Danon Aubin Silvere Djiwha
    AU  - Fatogoma Sorho
    Y1  - 2022/12/08
    PY  - 2022
    N1  - https://doi.org/10.11648/j.jps.20221006.16
    DO  - 10.11648/j.jps.20221006.16
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 235
    EP  - 240
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20221006.16
    AB  - Rice is the third most produced cereal in the world after maize and wheat. In Côte d'Ivoire, rice is the main food of the population. However, despite its importance, rice cultivation is subject to biotic constraints, including rice yellow mottle virus (RYMV), which is the most damaging viral disease of rice native to Africa. RYMV is a highly variable virus. This study aims to assess the ability of RYMV isolates to overcome the resistance-breaking genes RYMV1, RYMV2 and RYMV3. Sixteen RYMV isolates from Côte d'Ivoire representative of the agro-ecological zones (AEZ) visited and the varieties Bouake 189, Gigante, Tog 5681, Tog 5674, Tog 7291 and Tog 5307, carrying respectively rymv1-1 rymv1-2, rymv1-3, rymv1-5, RYMV2 and RYMV3 alleles were used were used. Varieties were sown in trays filled with sterilized soil so as to keep six plants per variety, i.e. 36 plants per tray per isolate. Rice plants were inoculated mechanically 14 days after sowing. Leaves of infected plants were collected individually per variety 45 days after inoculation in order to determine the virus concentration by serological DAS-ELISA tests. Asymptomatic infections were also investigated. The results showed that 37.5% of the tested isolates broke only the rymv1-2 gene with the presence of apparent symptoms. However, 12.5% of the isolates are infected both Gigante and Tog5674. None of the 16 isolates tested could induce symptoms in Tog5703, Tog5681 and Tog7291. Furthermore, only Tog7291 are showed the presence of the virus without apparent symptoms on rice leaves after inoculation. The identification of such isolates in rice-growing areas raises a serious threat to Ivorian rice production and calls for surveillance of this disease in Côte d'Ivoire.
    VL  - 10
    IS  - 6
    ER  - 

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Author Information
  • Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Pedagogical and Research Unit of Plant Physiology and Pathology, UFR Biosciences, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

  • National Center for Agricultural Research, Ferkessedougou Research Station, Ferkessedougou, C?te d’Ivoire

  • Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Pedagogical and Research Unit of Plant Physiology and Pathology, UFR Biosciences, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

  • Afrique Pesage, Abidjan, C?te d’Ivoire

  • Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Pedagogical and Research Unit of Plant Physiology and Pathology, UFR Biosciences, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

  • Department of Entomology, Faculty of Natural Sciences, Nangui Abrogoua University, Abidjan, C?te d’Ivoire

  • Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Pedagogical and Research Unit of Plant Physiology and Pathology, UFR Biosciences, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

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