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Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins

Received: 8 September 2016     Accepted: 7 February 2017     Published: 14 March 2017
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Abstract

Ten accessions of Pigeon pea (Cajanuscajan) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the ten accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions PP2, PP3, PP5, PP6, PP8 and PP9 have molecular weights not significantly different from one another (P<0.05) while samples PP1, PP4 and PP7 showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the ten accessions of pigeon pea (Cajanuscajan).

Published in Journal of Plant Sciences (Volume 5, Issue 2)
DOI 10.11648/j.jps.20170502.11
Page(s) 48-55
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Accessions, Gel Electrophoresis, Intra-Specific, Germplasm, Genetic Diversity

References
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Cite This Article
  • APA Style

    Agbolade James Oludare, Adekoya Modinat Adejoke, David Oyinade Aderoju, Chukwuma Deborah Moradeke, Komolafe Ronke Justina, et al. (2017). Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins. Journal of Plant Sciences, 5(2), 48-55. https://doi.org/10.11648/j.jps.20170502.11

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    ACS Style

    Agbolade James Oludare; Adekoya Modinat Adejoke; David Oyinade Aderoju; Chukwuma Deborah Moradeke; Komolafe Ronke Justina, et al. Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins. J. Plant Sci. 2017, 5(2), 48-55. doi: 10.11648/j.jps.20170502.11

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    AMA Style

    Agbolade James Oludare, Adekoya Modinat Adejoke, David Oyinade Aderoju, Chukwuma Deborah Moradeke, Komolafe Ronke Justina, et al. Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins. J Plant Sci. 2017;5(2):48-55. doi: 10.11648/j.jps.20170502.11

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  • @article{10.11648/j.jps.20170502.11,
      author = {Agbolade James Oludare and Adekoya Modinat Adejoke and David Oyinade Aderoju and Chukwuma Deborah Moradeke and Komolafe Ronke Justina and Olaiya Aderonke Eunice},
      title = {Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins},
      journal = {Journal of Plant Sciences},
      volume = {5},
      number = {2},
      pages = {48-55},
      doi = {10.11648/j.jps.20170502.11},
      url = {https://doi.org/10.11648/j.jps.20170502.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20170502.11},
      abstract = {Ten accessions of Pigeon pea (Cajanuscajan) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the ten accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions PP2, PP3, PP5, PP6, PP8 and PP9 have molecular weights not significantly different from one another (P0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the ten accessions of pigeon pea (Cajanuscajan).},
     year = {2017}
    }
    

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    T1  - Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins
    AU  - Agbolade James Oludare
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    AU  - David Oyinade Aderoju
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    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
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    EP  - 55
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    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20170502.11
    AB  - Ten accessions of Pigeon pea (Cajanuscajan) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the ten accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions PP2, PP3, PP5, PP6, PP8 and PP9 have molecular weights not significantly different from one another (P0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the ten accessions of pigeon pea (Cajanuscajan).
    VL  - 5
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Author Information
  • Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria

  • Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria

  • Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria

  • Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria

  • Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria

  • Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria

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