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Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106

Received: 10 September 2015     Accepted: 14 April 2016     Published: 11 May 2016
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Abstract

Malling Increasing demands for apple across the world requires its rapid production. Agricultural biotechnology has met this need and has prevented some problems during adolescence period and long generation. We investigated embryonic and non-embryonic callus through cultivating apical and lateral buds on different treatments with MS base culture and BAP (0-2.5 mg/l) and IBA (0-5 mg/l) hormones Kin and NAA hormones. The highest percent of embryogenesis related to T11 treatment (IBA 4 mg/l, BAP 1 mg/l) and T23 (IBA 4 mg/l, BAP 2.5 mg/l). The embryos were in corpuscular stage. Then third sub-culture was performed and all calluses and embryos were delivered to their corresponding treatments. K5 (NAA 1 mg/l, Kin 0.5 mg/l) and K8 (NAA 3 mg/l, Kin 1 mg/l) yielded the highest amount of callus (5.3 mm). The embryos remained in corpuscular stage and they did not show reproductive ability and developmental process. After preparation of artificial seed no regeneration was seen. By embryogenesis of MM.106 and M.26, the purpose of this research is improving a protocol for generation of two somatic cultivars.

Published in Journal of Plant Sciences (Volume 4, Issue 3)
DOI 10.11648/j.jps.20160403.13
Page(s) 46-51
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Malling, Synthetic Seed, Tissue Culture

References
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  • APA Style

    Zarinkamar Elham, Nejad Satari Taher. (2016). Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106. Journal of Plant Sciences, 4(3), 46-51. https://doi.org/10.11648/j.jps.20160403.13

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    ACS Style

    Zarinkamar Elham; Nejad Satari Taher. Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106. J. Plant Sci. 2016, 4(3), 46-51. doi: 10.11648/j.jps.20160403.13

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    AMA Style

    Zarinkamar Elham, Nejad Satari Taher. Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106. J Plant Sci. 2016;4(3):46-51. doi: 10.11648/j.jps.20160403.13

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  • @article{10.11648/j.jps.20160403.13,
      author = {Zarinkamar Elham and Nejad Satari Taher},
      title = {Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106},
      journal = {Journal of Plant Sciences},
      volume = {4},
      number = {3},
      pages = {46-51},
      doi = {10.11648/j.jps.20160403.13},
      url = {https://doi.org/10.11648/j.jps.20160403.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20160403.13},
      abstract = {Malling Increasing demands for apple across the world requires its rapid production. Agricultural biotechnology has met this need and has prevented some problems during adolescence period and long generation. We investigated embryonic and non-embryonic callus through cultivating apical and lateral buds on different treatments with MS base culture and BAP (0-2.5 mg/l) and IBA (0-5 mg/l) hormones Kin and NAA hormones. The highest percent of embryogenesis related to T11 treatment (IBA 4 mg/l, BAP 1 mg/l) and T23 (IBA 4 mg/l, BAP 2.5 mg/l). The embryos were in corpuscular stage. Then third sub-culture was performed and all calluses and embryos were delivered to their corresponding treatments. K5 (NAA 1 mg/l, Kin 0.5 mg/l) and K8 (NAA 3 mg/l, Kin 1 mg/l) yielded the highest amount of callus (5.3 mm). The embryos remained in corpuscular stage and they did not show reproductive ability and developmental process. After preparation of artificial seed no regeneration was seen. By embryogenesis of MM.106 and M.26, the purpose of this research is improving a protocol for generation of two somatic cultivars.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106
    AU  - Zarinkamar Elham
    AU  - Nejad Satari Taher
    Y1  - 2016/05/11
    PY  - 2016
    N1  - https://doi.org/10.11648/j.jps.20160403.13
    DO  - 10.11648/j.jps.20160403.13
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 46
    EP  - 51
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20160403.13
    AB  - Malling Increasing demands for apple across the world requires its rapid production. Agricultural biotechnology has met this need and has prevented some problems during adolescence period and long generation. We investigated embryonic and non-embryonic callus through cultivating apical and lateral buds on different treatments with MS base culture and BAP (0-2.5 mg/l) and IBA (0-5 mg/l) hormones Kin and NAA hormones. The highest percent of embryogenesis related to T11 treatment (IBA 4 mg/l, BAP 1 mg/l) and T23 (IBA 4 mg/l, BAP 2.5 mg/l). The embryos were in corpuscular stage. Then third sub-culture was performed and all calluses and embryos were delivered to their corresponding treatments. K5 (NAA 1 mg/l, Kin 0.5 mg/l) and K8 (NAA 3 mg/l, Kin 1 mg/l) yielded the highest amount of callus (5.3 mm). The embryos remained in corpuscular stage and they did not show reproductive ability and developmental process. After preparation of artificial seed no regeneration was seen. By embryogenesis of MM.106 and M.26, the purpose of this research is improving a protocol for generation of two somatic cultivars.
    VL  - 4
    IS  - 3
    ER  - 

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Author Information
  • Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

  • Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

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