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Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers

Received: 27 June 2015     Accepted: 4 July 2015     Published: 14 July 2015
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Abstract

Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.

Published in Journal of Plant Sciences (Volume 3, Issue 4)
DOI 10.11648/j.jps.20150304.14
Page(s) 191-196
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Cold-Induced Sweetening, Potato, Solanum tuberosum, SSR, Genetic Diversity

References
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Cite This Article
  • APA Style

    Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, et al. (2015). Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. Journal of Plant Sciences, 3(4), 191-196. https://doi.org/10.11648/j.jps.20150304.14

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    ACS Style

    Galani Yamdeu Joseph Hubert; Pooja H. Gupta; Nilesh J. Patel; Avadh K. Shah; Rajeshkumar R. Acharya, et al. Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. J. Plant Sci. 2015, 3(4), 191-196. doi: 10.11648/j.jps.20150304.14

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    AMA Style

    Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, et al. Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. J Plant Sci. 2015;3(4):191-196. doi: 10.11648/j.jps.20150304.14

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  • @article{10.11648/j.jps.20150304.14,
      author = {Galani Yamdeu Joseph Hubert and Pooja H. Gupta and Nilesh J. Patel and Avadh K. Shah and Rajeshkumar R. Acharya and Jayantkumar G. Talati},
      title = {Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers},
      journal = {Journal of Plant Sciences},
      volume = {3},
      number = {4},
      pages = {191-196},
      doi = {10.11648/j.jps.20150304.14},
      url = {https://doi.org/10.11648/j.jps.20150304.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150304.14},
      abstract = {Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers
    AU  - Galani Yamdeu Joseph Hubert
    AU  - Pooja H. Gupta
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    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
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    EP  - 196
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20150304.14
    AB  - Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.
    VL  - 3
    IS  - 4
    ER  - 

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Author Information
  • Department of Agriculture and Veterinary Medicine, Université des Montagnes, Bangangté, Cameroon

  • Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India

  • Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India

  • Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India

  • Main Vegetable Research Station, Anand Agricultural University, Gujarat, India

  • Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India

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