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Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers

Received: 10 July 2014     Accepted: 30 July 2014     Published: 20 September 2014
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Abstract

A total of twenty leaf rust resistance genes (Lr genes) were postulated in nine Egyptian wheat cultivars based on infection types (ITs) expressed on the tested cultivars by 72 Puccinia triticina pathotypes compared with the ITs expressed on the monogenic lines. The most carrier genes cultivars were Giza168 and Misr1 each may contain five genes i.e. Lr2c, 10, 18, 24, 41 and Lr3, 10, 19, 22b, 24, respectively. Five cultivars, Sakha94, Gemmeiza9, Gemmeiza10, Sids12 and Misr2 each probably contain four genes i.e. Lr9, 19, 29, 37; Lr18, 21, 24, 41; Lr3, 9, 19, 29; Lr9, 19, 26, 29 and Lr3, 10, 19, 26, respectively. Gemmeiza11 was the least cultivar carrying genes; it probably carries just two genes i.e. Lr24 and Lr41. The most postulated genes were Lr19 and Lr24, each postulated within five cultivars followed by Lr41 within four cultivars. Five Lr genes, Lr3, Lr9, Lr10, Lr26 and Lr29 each within three cultivars. The lowest postulated genes were Lr2c, Lr21, Lr22b and Lr37 each of them was postulated within only one cultivar. Five Lr genes, Lr9, Lr10, Lr19, Lr24 and Lr26 were identified by PCR-based molecular marker. The Lr9 gene was identified in cultivar Sids12 while, Lr10 was identified in cultivar Misr1. The Lr19 was present in two cultivars, Misr1 and Misr2. The Lr24 and Lr26 were absent in all the screened Egyptian cultivars. The obtained results for Lr9, Lr10, Lr19, Lr24 and Lr26 marker were in agreement with and confirm their identification by gene postulation. Markers for Lr9, Lr10 and Lr19 may be useful in marker-assisted breeding. Our findings showed the usefulness of the molecular marker in identifying leaf rust resistance genes in wheat cultivars, especially when used in conjunction with multipathotypes test at the pre-breeding stage. This approach may help understanding the wheat - P. triticina interaction and provide information to build an effective management program for leaf rust disease.

Published in Journal of Plant Sciences (Volume 2, Issue 5)
DOI 10.11648/j.jps.20140205.11
Page(s) 145-151
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Leaf Rust, Puccinia triticina, Multipathotypes, Gene Postulation, Wheat Lr-Genes, Lr-Genes Marker, Molecular Analysis

References
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Cite This Article
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    Mohammed Saad Abou-Elseoud, Abd-Elmageed Mohammed Kamara, Omaima Abd-Ellatif Alaa-Eldein, Ahmed Farag El-Bebany, Nader Abd-Elwahab Ashmawy, et al. (2014). Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers. Journal of Plant Sciences, 2(5), 145-151. https://doi.org/10.11648/j.jps.20140205.11

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    Mohammed Saad Abou-Elseoud; Abd-Elmageed Mohammed Kamara; Omaima Abd-Ellatif Alaa-Eldein; Ahmed Farag El-Bebany; Nader Abd-Elwahab Ashmawy, et al. Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers. J. Plant Sci. 2014, 2(5), 145-151. doi: 10.11648/j.jps.20140205.11

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    Mohammed Saad Abou-Elseoud, Abd-Elmageed Mohammed Kamara, Omaima Abd-Ellatif Alaa-Eldein, Ahmed Farag El-Bebany, Nader Abd-Elwahab Ashmawy, et al. Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers. J Plant Sci. 2014;2(5):145-151. doi: 10.11648/j.jps.20140205.11

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  • @article{10.11648/j.jps.20140205.11,
      author = {Mohammed Saad Abou-Elseoud and Abd-Elmageed Mohammed Kamara and Omaima Abd-Ellatif Alaa-Eldein and Ahmed Farag El-Bebany and Nader Abd-Elwahab Ashmawy and Ibrahim Sobhy Draz},
      title = {Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers},
      journal = {Journal of Plant Sciences},
      volume = {2},
      number = {5},
      pages = {145-151},
      doi = {10.11648/j.jps.20140205.11},
      url = {https://doi.org/10.11648/j.jps.20140205.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20140205.11},
      abstract = {A total of twenty leaf rust resistance genes (Lr genes) were postulated in nine Egyptian wheat cultivars based on infection types (ITs) expressed on the tested cultivars by 72 Puccinia triticina pathotypes compared with the ITs expressed on the monogenic lines. The most carrier genes cultivars were Giza168 and Misr1 each may contain five genes i.e. Lr2c, 10, 18, 24, 41 and Lr3, 10, 19, 22b, 24, respectively. Five cultivars, Sakha94, Gemmeiza9, Gemmeiza10, Sids12 and Misr2 each probably contain four genes i.e. Lr9, 19, 29, 37; Lr18, 21, 24, 41; Lr3, 9, 19, 29; Lr9, 19, 26, 29 and Lr3, 10, 19, 26, respectively. Gemmeiza11 was the least cultivar carrying genes; it probably carries just two genes i.e. Lr24 and Lr41. The most postulated genes were Lr19 and Lr24, each postulated within five cultivars followed by Lr41 within four cultivars. Five Lr genes, Lr3, Lr9, Lr10, Lr26 and Lr29 each within three cultivars. The lowest postulated genes were Lr2c, Lr21, Lr22b and Lr37 each of them was postulated within only one cultivar. Five Lr genes, Lr9, Lr10, Lr19, Lr24 and Lr26 were identified by PCR-based molecular marker. The Lr9 gene was identified in cultivar Sids12 while, Lr10 was identified in cultivar Misr1. The Lr19 was present in two cultivars, Misr1 and Misr2. The Lr24 and Lr26 were absent in all the screened Egyptian cultivars. The obtained results for Lr9, Lr10, Lr19, Lr24 and Lr26 marker were in agreement with and confirm their identification by gene postulation. Markers for Lr9, Lr10 and Lr19 may be useful in marker-assisted breeding. Our findings showed the usefulness of the molecular marker in identifying leaf rust resistance genes in wheat cultivars, especially when used in conjunction with multipathotypes test at the pre-breeding stage. This approach may help understanding the wheat - P. triticina interaction and provide information to build an effective management program for leaf rust disease.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers
    AU  - Mohammed Saad Abou-Elseoud
    AU  - Abd-Elmageed Mohammed Kamara
    AU  - Omaima Abd-Ellatif Alaa-Eldein
    AU  - Ahmed Farag El-Bebany
    AU  - Nader Abd-Elwahab Ashmawy
    AU  - Ibrahim Sobhy Draz
    Y1  - 2014/09/20
    PY  - 2014
    N1  - https://doi.org/10.11648/j.jps.20140205.11
    DO  - 10.11648/j.jps.20140205.11
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 145
    EP  - 151
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20140205.11
    AB  - A total of twenty leaf rust resistance genes (Lr genes) were postulated in nine Egyptian wheat cultivars based on infection types (ITs) expressed on the tested cultivars by 72 Puccinia triticina pathotypes compared with the ITs expressed on the monogenic lines. The most carrier genes cultivars were Giza168 and Misr1 each may contain five genes i.e. Lr2c, 10, 18, 24, 41 and Lr3, 10, 19, 22b, 24, respectively. Five cultivars, Sakha94, Gemmeiza9, Gemmeiza10, Sids12 and Misr2 each probably contain four genes i.e. Lr9, 19, 29, 37; Lr18, 21, 24, 41; Lr3, 9, 19, 29; Lr9, 19, 26, 29 and Lr3, 10, 19, 26, respectively. Gemmeiza11 was the least cultivar carrying genes; it probably carries just two genes i.e. Lr24 and Lr41. The most postulated genes were Lr19 and Lr24, each postulated within five cultivars followed by Lr41 within four cultivars. Five Lr genes, Lr3, Lr9, Lr10, Lr26 and Lr29 each within three cultivars. The lowest postulated genes were Lr2c, Lr21, Lr22b and Lr37 each of them was postulated within only one cultivar. Five Lr genes, Lr9, Lr10, Lr19, Lr24 and Lr26 were identified by PCR-based molecular marker. The Lr9 gene was identified in cultivar Sids12 while, Lr10 was identified in cultivar Misr1. The Lr19 was present in two cultivars, Misr1 and Misr2. The Lr24 and Lr26 were absent in all the screened Egyptian cultivars. The obtained results for Lr9, Lr10, Lr19, Lr24 and Lr26 marker were in agreement with and confirm their identification by gene postulation. Markers for Lr9, Lr10 and Lr19 may be useful in marker-assisted breeding. Our findings showed the usefulness of the molecular marker in identifying leaf rust resistance genes in wheat cultivars, especially when used in conjunction with multipathotypes test at the pre-breeding stage. This approach may help understanding the wheat - P. triticina interaction and provide information to build an effective management program for leaf rust disease.
    VL  - 2
    IS  - 5
    ER  - 

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Author Information
  • Department of Plant Pathology, Faculty of Agriculture, Alexandria University, El-Shatby 21545, Alexandria, Egypt

  • Department of Plant Pathology, Faculty of Agriculture, Alexandria University, El-Shatby 21545, Alexandria, Egypt

  • Wheat Diseases Research Department, Plant Pathology Research Institute, PPRI, Agricultural Research Centre, Giza 12619, Egypt

  • Department of Plant Pathology, Faculty of Agriculture, Alexandria University, El-Shatby 21545, Alexandria, Egypt

  • Department of Plant Pathology, Faculty of Agriculture, Alexandria University, El-Shatby 21545, Alexandria, Egypt

  • Wheat Diseases Research Department, Plant Pathology Research Institute, PPRI, Agricultural Research Centre, Giza 12619, Egypt

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