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Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats

Received: 8 March 2023     Accepted: 6 April 2023     Published: 27 April 2023
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Abstract

Anemia is characterized by an abnormal decrease in the level of hemoglobin or the number of red blood cells in the blood. It is a public health problem that mainly affects pregnant women and young children. In the present study, the objective was to evaluated the anti-anemic potential of a recipe composed of three medicinal plants, Xylopia aethiopica (Dunal) A. Rich, Zanthoxylum leprieurii (GUILL) and Harungara madagascariensis (LAM), used to treat sickle cell anemia in rats. Aqueous and hydroethanol extracts of recipe composed were first prepared and quantitative phytochemical tests were carried out. Then, the anti-anemic activity of the recipe extracts was assessed. The anemia was induced in animals by intraperitoneal injection of phenylhydrazine at a repeated dose of 40 mg/kg bw for two days. Treatment was carried out with the aqueous and hydroethanol extracts of the plant recipe at doses of 400 and 800 mg/kg bw. The obtained results indicated that recipe composed contains total phenols (43.31±0.92 mg GAE/g and 33.82±0.6 mg GAE /g of dry weight), total flavonoids (17.07±0.89 mg QE and 19.76±1.15 mg QE/g of dry weight) and total tannin (8.65±0.01 mg QE and 26.5±0.95 mg Cat E/g of dry weight) in decocted and hydroethanol extracts respectively. The anemia was effectively corrected after 21 days of oral administration of the extracts to the rats. The extracts significantly increased hemoglobin (P < 0.05), red blood cell count (P < 0.001), and hematocrit (P < 0.01) as early as the 7th days of treatment compared with untreated controls. These results show that the plant recipe composed of Xylopia aethiopica, Zanthoxylum leprieurii and Harungara madagascariensis possess antianemic properties.

Published in Journal of Diseases and Medicinal Plants (Volume 9, Issue 2)
DOI 10.11648/j.jdmp.20230902.12
Page(s) 40-48
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Anemia, Antianemic Potential, Phenylhydrazine, Medicinal Plants

References
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Cite This Article
  • APA Style

    Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Konan Armand Marcelin Kouassi, Sitapha Ouattara, Gbe Kouakou N’Dri Ange Konan, et al. (2023). Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats. Journal of Diseases and Medicinal Plants, 9(2), 40-48. https://doi.org/10.11648/j.jdmp.20230902.12

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    Tatiana Kangah Mireille Kple; Joel Akakpo-Akue; Konan Armand Marcelin Kouassi; Sitapha Ouattara; Gbe Kouakou N’Dri Ange Konan, et al. Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats. J. Dis. Med. Plants 2023, 9(2), 40-48. doi: 10.11648/j.jdmp.20230902.12

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    AMA Style

    Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Konan Armand Marcelin Kouassi, Sitapha Ouattara, Gbe Kouakou N’Dri Ange Konan, et al. Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats. J Dis Med Plants. 2023;9(2):40-48. doi: 10.11648/j.jdmp.20230902.12

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  • @article{10.11648/j.jdmp.20230902.12,
      author = {Tatiana Kangah Mireille Kple and Joel Akakpo-Akue and Konan Armand Marcelin Kouassi and Sitapha Ouattara and Gbe Kouakou N’Dri Ange Konan and Diane Koulai and Larissa Adjoua Kouadio and Chiaye Claire Antoinette Yapo-Crezoit and Adou Koffi Mattieu Kra and Allico Joseph Djaman},
      title = {Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats},
      journal = {Journal of Diseases and Medicinal Plants},
      volume = {9},
      number = {2},
      pages = {40-48},
      doi = {10.11648/j.jdmp.20230902.12},
      url = {https://doi.org/10.11648/j.jdmp.20230902.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20230902.12},
      abstract = {Anemia is characterized by an abnormal decrease in the level of hemoglobin or the number of red blood cells in the blood. It is a public health problem that mainly affects pregnant women and young children. In the present study, the objective was to evaluated the anti-anemic potential of a recipe composed of three medicinal plants, Xylopia aethiopica (Dunal) A. Rich, Zanthoxylum leprieurii (GUILL) and Harungara madagascariensis (LAM), used to treat sickle cell anemia in rats. Aqueous and hydroethanol extracts of recipe composed were first prepared and quantitative phytochemical tests were carried out. Then, the anti-anemic activity of the recipe extracts was assessed. The anemia was induced in animals by intraperitoneal injection of phenylhydrazine at a repeated dose of 40 mg/kg bw for two days. Treatment was carried out with the aqueous and hydroethanol extracts of the plant recipe at doses of 400 and 800 mg/kg bw. The obtained results indicated that recipe composed contains total phenols (43.31±0.92 mg GAE/g and 33.82±0.6 mg GAE /g of dry weight), total flavonoids (17.07±0.89 mg QE and 19.76±1.15 mg QE/g of dry weight) and total tannin (8.65±0.01 mg QE and 26.5±0.95 mg Cat E/g of dry weight) in decocted and hydroethanol extracts respectively. The anemia was effectively corrected after 21 days of oral administration of the extracts to the rats. The extracts significantly increased hemoglobin (P th days of treatment compared with untreated controls. These results show that the plant recipe composed of Xylopia aethiopica, Zanthoxylum leprieurii and Harungara madagascariensis possess antianemic properties.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats
    AU  - Tatiana Kangah Mireille Kple
    AU  - Joel Akakpo-Akue
    AU  - Konan Armand Marcelin Kouassi
    AU  - Sitapha Ouattara
    AU  - Gbe Kouakou N’Dri Ange Konan
    AU  - Diane Koulai
    AU  - Larissa Adjoua Kouadio
    AU  - Chiaye Claire Antoinette Yapo-Crezoit
    AU  - Adou Koffi Mattieu Kra
    AU  - Allico Joseph Djaman
    Y1  - 2023/04/27
    PY  - 2023
    N1  - https://doi.org/10.11648/j.jdmp.20230902.12
    DO  - 10.11648/j.jdmp.20230902.12
    T2  - Journal of Diseases and Medicinal Plants
    JF  - Journal of Diseases and Medicinal Plants
    JO  - Journal of Diseases and Medicinal Plants
    SP  - 40
    EP  - 48
    PB  - Science Publishing Group
    SN  - 2469-8210
    UR  - https://doi.org/10.11648/j.jdmp.20230902.12
    AB  - Anemia is characterized by an abnormal decrease in the level of hemoglobin or the number of red blood cells in the blood. It is a public health problem that mainly affects pregnant women and young children. In the present study, the objective was to evaluated the anti-anemic potential of a recipe composed of three medicinal plants, Xylopia aethiopica (Dunal) A. Rich, Zanthoxylum leprieurii (GUILL) and Harungara madagascariensis (LAM), used to treat sickle cell anemia in rats. Aqueous and hydroethanol extracts of recipe composed were first prepared and quantitative phytochemical tests were carried out. Then, the anti-anemic activity of the recipe extracts was assessed. The anemia was induced in animals by intraperitoneal injection of phenylhydrazine at a repeated dose of 40 mg/kg bw for two days. Treatment was carried out with the aqueous and hydroethanol extracts of the plant recipe at doses of 400 and 800 mg/kg bw. The obtained results indicated that recipe composed contains total phenols (43.31±0.92 mg GAE/g and 33.82±0.6 mg GAE /g of dry weight), total flavonoids (17.07±0.89 mg QE and 19.76±1.15 mg QE/g of dry weight) and total tannin (8.65±0.01 mg QE and 26.5±0.95 mg Cat E/g of dry weight) in decocted and hydroethanol extracts respectively. The anemia was effectively corrected after 21 days of oral administration of the extracts to the rats. The extracts significantly increased hemoglobin (P th days of treatment compared with untreated controls. These results show that the plant recipe composed of Xylopia aethiopica, Zanthoxylum leprieurii and Harungara madagascariensis possess antianemic properties.
    VL  - 9
    IS  - 2
    ER  - 

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Author Information
  • Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

  • Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

  • Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

  • Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

  • Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

  • Food and Bioproducts Processes Laboratory, Department of Agronomic, Forestry and Environmental Engineering, University of Man, Man, C?te d’Ivoire

  • Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

  • Immunity Biology Pole, Pasteur Institute, Abidjan, C?te d’Ivoire

  • Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

  • Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

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