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The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells

Received: 15 December 2021     Accepted: 27 December 2021     Published: 28 January 2022
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Abstract

Background: Traditional Mongolian medicine Eerdun Wurile is one of the ideal drugs for cerebral stroke and is effective on treating vascular and nervous system diseases. Numerous studies have proved that Eerdun Wurile has neuroprotective effect. However, the biological mechanism of which is still unclear. Objective: To investigatethe molecular mechanism underlying the neuroprotective effect of Eerdun Wurile. Methods: The extract from Eerdun Wurile was prepared by simulated gastric fluid first and its effect on PC12 cells differentiaton and MAPK phosphorylation was tested by neurite outgrowth assay and western blotting. Also, the target of active compounds was predicted by moleculr modeling system and the content of active compounds was examined by LC-MS/MS. Result: Result showed that the extract stimulated the differentiation of PC12 cells, exhibited nerve growth factor (NGF)-like effect. Meanwhile, the extract induced the MAPK phosphorylation in PC12 cells. In-depth analysis by molecular modeling technology predicted that genipin interact with MAPK as a target. Consistent with the prediction, genipin induced the phosphorylation of MAPK in PC12 cells. Besides, genipin was detected both in the extract and Eerdun Wurile pills. Conclusion: These results proved that the extract of Eerdun Wurile has neuroprotective effect in PC12 cells, and genipin in the extract and Eerdun Wurile might be one of the active compounds that show neuroprotective effect.

Published in International Journal of Pharmacy and Chemistry (Volume 8, Issue 1)
DOI 10.11648/j.ijpc.20220801.12
Page(s) 5-11
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Traditional Mongolian Medicine Eerdun Wurile, Molecular Modeling Technology, Genipin, MAPK, PC12 Cells

References
[1] Bao Li-li, SONG Fu-shun, Dugeer et al. The progress of traditional Mongolian medicine Erden-uril. Chinese Journal of Ethnomedicine and Ethnopharmacy, 2019, 28 (3): 53-56 (Chinese).
[2] Eerdundalai. Effect of Eerdun Wurile on inflammatory response and oxidative damage induced by MCAO/R in rats [D]. Inner Mongolia Medical University, 2019 (Chinese).
[3] Sun Yan-xia, Gu-hao, Therapeutic effect of Mongolian medicine Erdun wurile on cerebral infarction and NIHSS score. Journal of Medicine & Pharmacy of Chinese Minorities. 2019, 25 (10): 13-14.(Chinese).
[4] Liu jian, Zhao fu-quan, Clinical efficacy of Mongolian medicine Erdun wurile in the treatment of schemic stroke during recovery stage. Journal of Medicine & Pharmacy of Chinese Minorities. 2020, 26 (12): 7-9.(Chinese).
[5] Sarula, Study on the mechanism of Mongolian medicine Erdun Wurile regulating microglia in MCAO/R injury model rats. [D]. Inner Mongolia University for Nationalities. 2020. (Chinese).
[6] Gaowa S, Bao N, Da M, Qiburi Q, Ganbold T, Chen L, Altangerel A, Temuqile T, Baigude H. Traditional Mongolian medicine Eerdun Wurile improves stroke recovery through regulation of gene expression in rat brain. J Ethnopharmacol. 2018; 222: 249-260.
[7] Du Ri-na. Protective mechanism of Eerdun-wurile on retinal ischemia-reperfusion injury in rats [D]. Inner Mongolia Medical University, 2019 (Chinese).
[8] Zhang Xing, Ma Chun-jie, Lian Hua, et al. Effect of Eerdun Wurile preconditioning on apoptosis after myocardial ischemia-reperfusion injury in rats. Liao-ning Journal of Traditional Chinese Medicie. 2020, 47 (05): 183-186+221. (Chinese).
[9] Yan Qing-zhi, Ma Chun-jie, Hao Qiang-wei et al. Effects of Mongolian Medicine E'erdun-Wurile Preconditioning on Autophagy-Related Protein Expression in Myocardial Ischemia-Reperfusion Injury Rats. Journal of Traditional Chinese Medicine. 2019, 60 (04): 333-338. (Chinese).
[10] Astrid Obmanna, Damba Tsendayushb, Theresia Thalhammerc etc,. Extracts from the Mongolian traditional medicinal plants Dianthus versicolor Fisch and Lilium pumilum Delile stimulate bile flow in an isolated perfused rat liver model. Journal of Ethnopharmacology. 2010; 131: 555-561.
[11] Astrid Obmann, Martin Zeh, Sodnomtseren Purevsuren, et al. Quantification of flavonoid glycosides in an aqueous extract from the traditional Mongolian medicinal plant Dianthus versicolor FISCH. J. Sep. Sci. 2011, 34, 292–298.
[12] A Run, Wu Feng-qiao, Wang Xiu-lan et al. The research progress of pharmacological activity and application of Gardenia jasminoides in Chinese and Mongolian medicine. Chinese Traditional Patent Medicine. 2021, 43 (02): 459-463 (Chinese).
[13] Shanmugam MK, Shen H, Tang FR, Arfuso F, Rajesh M, Wang L, Kumar AP, Bian J, Goh BC, Bishayee A, Sethi G. Potential role of genipin in cancer therapy. Pharmacol Res. 2018; 133: 195-200.
[14] Wang J, Chen L, Liang Z, Li Y, Yuan F, Liu J, Tian Y, Hao Z, Zhou F, Liu X, Cao Y, Zheng Y, Li Q. Genipin Inhibits LPS-Induced Inflammatory Response in BV2 Microglial Cells. Neurochem Res. 2017; 42 (10): 2769-2776.
[15] Li Z, Zhang TB, Jia DH, Sun WQ, Wang CL, Gu AZ, Yang XM. Genipin inhibits the growth of human bladder cancer cells via inactivation of PI3K/Akt signaling. Oncol Lett. 2018; 15 (2): 2619-2624.
[16] Jo MJ, Jeong S, Yun HK, Kim DY, Kim BR, Kim JL, Na YJ, Park SH, Jeong YA, Kim BG, Ashktorab H, Smoot DT, Heo JY, Han J, Lee DH, Oh SC. Genipin induces mitochondrial dysfunction and apoptosis via downregulation of Stat3/mcl-1 pathway in gastric cancer. BMC Cancer. 2019; 19 (1): 739.
[17] Ye J, Li J, Wang X, Li L. Medicinal supplement genipin induces p53 and Bax-dependent apoptosis in colon cancer cells. Oncol Lett. 2018; 16 (3): 2957-2964.
[18] Lee JH, Cho YS, Jung KH, Park JW, Lee KH. Genipin enhances the antitumor effect of elesclomol in A549 lung cancer cells by blocking uncoupling protein-2 and stimulating reactive oxygen species production. Oncol Lett. 2020; 20 (6): 374.
[19] Kim BR, Jeong YA, Jo MJ, Park SH, Na YJ, Kim JL, Jeong S, Yun HK, Kang S, Lee DH, Oh SC. Genipin Enhances the Therapeutic Effects of Oxaliplatin by Upregulating BIM in Colorectal Cancer. Mol Cancer Ther. 2019; 18 (4): 751-761.
[20] Zhang CY, Parton LE, Ye CP, Krauss S, Shen R, Lin CT, Porco JA Jr, Lowell BB. Genipin inhibits UCP2-mediated proton leak and acutely reverses obesity- and high glucose-induced beta cell dysfunction in isolated pancreatic islets. Cell Metab. 2006; 3 (6): 417-27.
[21] Rajanbabu V, Galam L, Fukumoto J, Enciso J, Tadikonda P, Lane TN, Bandyopadhyay S, Parthasarathy PT, Cho Y, Cho SH, Lee YC, Lockey RF, Kolliputi N. Genipin suppresses NLRP3 inflammasome activation through uncoupling protein-2. Cell Immunol. 2015; 297 (1): 40-5.
[22] Zhong X, He J, Zhang X, Li C, Tian X, Xia W, Gan H, Xia Y. UCP2 alleviates tubular epithelial cell apoptosis in lipopolysaccharide-induced acute kidney injury by decreasing ROS production. Biomed Pharmacother. 2019; 115: 108914.
[23] Yu Y, Xu S, Li S, Pan H. Genipin-cross-linked hydrogels based on biomaterials for drug delivery: a review. Biomater Sci. 2021; 9 (5): 1583-1597.
[24] Wang M, Da L, Xie Y, Xie H. Application of genipin for modification of natural biomaterials as a crosslinking agent. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2013; 27 (5): 580-5 (Chinese).
[25] Wang Z, Liu H, Luo W, Cai T, Li Z, Liu Y, Gao W, Wan Q, Wang X, Wang J, Wang Y, Yang X. Regeneration of skeletal system with genipin crosslinked biomaterials. J Tissue Eng. 2020; 11: 2041731420974861.
[26] Thakur G, Rodrigues FC, Singh K. Crosslinking Biopolymers for Advanced Drug Delivery and Tissue Engineering Applications. Adv Exp Med Biol. 2018; 1078: 213-231.
[27] Li Y, Li L, Hölscher C. Therapeutic Potential of Genipin in Central Neurodegenerative Diseases. CNS Drugs. 2016; 30 (10): 889-97.
Cite This Article
  • APA Style

    Wang Qingliang, Bao Yinping, Sudun, Gaowa, Dugeer, et al. (2022). The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells. International Journal of Pharmacy and Chemistry, 8(1), 5-11. https://doi.org/10.11648/j.ijpc.20220801.12

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    ACS Style

    Wang Qingliang; Bao Yinping; Sudun; Gaowa; Dugeer, et al. The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells. Int. J. Pharm. Chem. 2022, 8(1), 5-11. doi: 10.11648/j.ijpc.20220801.12

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    AMA Style

    Wang Qingliang, Bao Yinping, Sudun, Gaowa, Dugeer, et al. The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells. Int J Pharm Chem. 2022;8(1):5-11. doi: 10.11648/j.ijpc.20220801.12

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  • @article{10.11648/j.ijpc.20220801.12,
      author = {Wang Qingliang and Bao Yinping and Sudun and Gaowa and Dugeer and Alatengqimuge and Li Hanqing and Song Fushun and Zhang Wen and Tianji and Bao Xilinqiqige and Tegexibaiyin and Bao Siqin and Wuhanqimuge},
      title = {The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells},
      journal = {International Journal of Pharmacy and Chemistry},
      volume = {8},
      number = {1},
      pages = {5-11},
      doi = {10.11648/j.ijpc.20220801.12},
      url = {https://doi.org/10.11648/j.ijpc.20220801.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20220801.12},
      abstract = {Background: Traditional Mongolian medicine Eerdun Wurile is one of the ideal drugs for cerebral stroke and is effective on treating vascular and nervous system diseases. Numerous studies have proved that Eerdun Wurile has neuroprotective effect. However, the biological mechanism of which is still unclear. Objective: To investigatethe molecular mechanism underlying the neuroprotective effect of Eerdun Wurile. Methods: The extract from Eerdun Wurile was prepared by simulated gastric fluid first and its effect on PC12 cells differentiaton and MAPK phosphorylation was tested by neurite outgrowth assay and western blotting. Also, the target of active compounds was predicted by moleculr modeling system and the content of active compounds was examined by LC-MS/MS. Result: Result showed that the extract stimulated the differentiation of PC12 cells, exhibited nerve growth factor (NGF)-like effect. Meanwhile, the extract induced the MAPK phosphorylation in PC12 cells. In-depth analysis by molecular modeling technology predicted that genipin interact with MAPK as a target. Consistent with the prediction, genipin induced the phosphorylation of MAPK in PC12 cells. Besides, genipin was detected both in the extract and Eerdun Wurile pills. Conclusion: These results proved that the extract of Eerdun Wurile has neuroprotective effect in PC12 cells, and genipin in the extract and Eerdun Wurile might be one of the active compounds that show neuroprotective effect.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells
    AU  - Wang Qingliang
    AU  - Bao Yinping
    AU  - Sudun
    AU  - Gaowa
    AU  - Dugeer
    AU  - Alatengqimuge
    AU  - Li Hanqing
    AU  - Song Fushun
    AU  - Zhang Wen
    AU  - Tianji
    AU  - Bao Xilinqiqige
    AU  - Tegexibaiyin
    AU  - Bao Siqin
    AU  - Wuhanqimuge
    Y1  - 2022/01/28
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ijpc.20220801.12
    DO  - 10.11648/j.ijpc.20220801.12
    T2  - International Journal of Pharmacy and Chemistry
    JF  - International Journal of Pharmacy and Chemistry
    JO  - International Journal of Pharmacy and Chemistry
    SP  - 5
    EP  - 11
    PB  - Science Publishing Group
    SN  - 2575-5749
    UR  - https://doi.org/10.11648/j.ijpc.20220801.12
    AB  - Background: Traditional Mongolian medicine Eerdun Wurile is one of the ideal drugs for cerebral stroke and is effective on treating vascular and nervous system diseases. Numerous studies have proved that Eerdun Wurile has neuroprotective effect. However, the biological mechanism of which is still unclear. Objective: To investigatethe molecular mechanism underlying the neuroprotective effect of Eerdun Wurile. Methods: The extract from Eerdun Wurile was prepared by simulated gastric fluid first and its effect on PC12 cells differentiaton and MAPK phosphorylation was tested by neurite outgrowth assay and western blotting. Also, the target of active compounds was predicted by moleculr modeling system and the content of active compounds was examined by LC-MS/MS. Result: Result showed that the extract stimulated the differentiation of PC12 cells, exhibited nerve growth factor (NGF)-like effect. Meanwhile, the extract induced the MAPK phosphorylation in PC12 cells. In-depth analysis by molecular modeling technology predicted that genipin interact with MAPK as a target. Consistent with the prediction, genipin induced the phosphorylation of MAPK in PC12 cells. Besides, genipin was detected both in the extract and Eerdun Wurile pills. Conclusion: These results proved that the extract of Eerdun Wurile has neuroprotective effect in PC12 cells, and genipin in the extract and Eerdun Wurile might be one of the active compounds that show neuroprotective effect.
    VL  - 8
    IS  - 1
    ER  - 

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Author Information
  • Mongolian Medical College, Inner Mongolia Medical University, Hohhot, China

  • Pharmaceutical Laboratory, Affiliated Hospital of Inner Mongolia University, Tongliao, China

  • Research and Development Center, Ruihua (Shenzhen) Intelligence Company Limited, Shenzhen, China

  • Mongolian Medical College, Inner Mongolia Medical University, Hohhot, China

  • Innovative Mongolian Medical Engineering Research Center, Inner Mongolia International Mongolian Hospital, Hohhot, China

  • Innovative Mongolian Medical Engineering Research Center, Inner Mongolia International Mongolian Hospital, Hohhot, China

  • Innovative Mongolian Medical Engineering Research Center, Inner Mongolia International Mongolian Hospital, Hohhot, China

  • Innovative Mongolian Medical Engineering Research Center, Inner Mongolia International Mongolian Hospital, Hohhot, China

  • Innovative Mongolian Medical Engineering Research Center, Inner Mongolia International Mongolian Hospital, Hohhot, China

  • Innovative Mongolian Medical Engineering Research Center, Inner Mongolia International Mongolian Hospital, Hohhot, China

  • Mongolian Medical College, Inner Mongolia Medical University, Hohhot, China

  • Innovative Mongolian Medical Engineering Research Center, Inner Mongolia International Mongolian Hospital, Hohhot, China

  • Innovative Mongolian Medical Engineering Research Center, Inner Mongolia International Mongolian Hospital, Hohhot, China

  • Innovative Mongolian Medical Engineering Research Center, Inner Mongolia International Mongolian Hospital, Hohhot, China

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