Rhizobia are diazotrophic bacteria that fix nitrogen after becoming established inside the root nodules of legumes (Fabaceae). This study was conducted to isolate, characterize and evaluate the symbiotic effectiveness of rhizobia nodulating common bean. Nodule sample were collected from six randomly selected kebele of the study area for nodule induction under controlled condition. At the flowering stage after 45 days of plant growth, root nodules were collected and rhizobia bacteria isolated. The isolates were allowed for different biochemical and physiological characterization. Presumptive tests confirmed that isolates were root nodulating rhizobia. Rhizobial inoculants (Bio-fertilizer) effect on the host crop was tested under greenhouse condition on sterilized river sand culture. Plant treated with KNO3 were set as positive and plants treated with sterile distilled water were used as negative control. Isolates treated plant group were experimental group. The experiment was laid in CRD design. After 45 days of inoculation different growth, related parameters were investigated. Collected data on growth related parameter after rhizobia inoculation were analyzed using SAS software version 9.1. Rhizobial treatment effect on plant growth related parameters were investigated. Effect of treatment was significant at (P<0.05) difference on SDW, NDW, SFW. SE. rhizobial treatment on host crop shown significant effect and rhizobia were capable to infect the host crop in sterile river sand culture under greenhouse condition. The isolates were highly effective on their symbiotic effectiveness rate. Therefore, the molecular characterization of the isolates of the area and effect of inoculant on yield related parameter of host crop (Phaseolus Vulgaris L.) study on field trial should be future perspective of the area.
| Published in | International Journal of Microbiology and Biotechnology (Volume 10, Issue 2) |
| DOI | 10.11648/j.ijmb.20251002.12 |
| Page(s) | 39-53 |
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Biological Nitrogen Fixation, Common Bean, Nitrogen, Nodulation and Rhizobia
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APA Style
Turito, A. A., I. Z., Nemomsa, N. E., Belew, A. A. (2025). Isolation, Characterization and Evaluation of Symbiotic Effectiveness of Rhizobia Nodulating (Phaseolus Vulgaris L) Nodules Collected from Gurafarda, Southwest Ethiopia. International Journal of Microbiology and Biotechnology, 10(2), 39-53. https://doi.org/10.11648/j.ijmb.20251002.12
ACS Style
Turito, A. A.; I. Z.; Nemomsa, N. E.; Belew, A. A. Isolation, Characterization and Evaluation of Symbiotic Effectiveness of Rhizobia Nodulating (Phaseolus Vulgaris L) Nodules Collected from Gurafarda, Southwest Ethiopia. Int. J. Microbiol. Biotechnol. 2025, 10(2), 39-53. doi: 10.11648/j.ijmb.20251002.12
AMA Style
Turito AA, IZ, Nemomsa NE, Belew AA. Isolation, Characterization and Evaluation of Symbiotic Effectiveness of Rhizobia Nodulating (Phaseolus Vulgaris L) Nodules Collected from Gurafarda, Southwest Ethiopia. Int J Microbiol Biotechnol. 2025;10(2):39-53. doi: 10.11648/j.ijmb.20251002.12
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Download@article{10.11648/j.ijmb.20251002.12,
author = {Andualem Arimo Turito and Israel Zewde and Nigatu Ebisa Nemomsa and Adugna Abdissa Belew},
title = {Isolation, Characterization and Evaluation of Symbiotic Effectiveness of Rhizobia Nodulating (Phaseolus Vulgaris L) Nodules Collected from Gurafarda, Southwest Ethiopia
},
journal = {International Journal of Microbiology and Biotechnology},
volume = {10},
number = {2},
pages = {39-53},
doi = {10.11648/j.ijmb.20251002.12},
url = {https://doi.org/10.11648/j.ijmb.20251002.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20251002.12},
abstract = {Rhizobia are diazotrophic bacteria that fix nitrogen after becoming established inside the root nodules of legumes (Fabaceae). This study was conducted to isolate, characterize and evaluate the symbiotic effectiveness of rhizobia nodulating common bean. Nodule sample were collected from six randomly selected kebele of the study area for nodule induction under controlled condition. At the flowering stage after 45 days of plant growth, root nodules were collected and rhizobia bacteria isolated. The isolates were allowed for different biochemical and physiological characterization. Presumptive tests confirmed that isolates were root nodulating rhizobia. Rhizobial inoculants (Bio-fertilizer) effect on the host crop was tested under greenhouse condition on sterilized river sand culture. Plant treated with KNO3 were set as positive and plants treated with sterile distilled water were used as negative control. Isolates treated plant group were experimental group. The experiment was laid in CRD design. After 45 days of inoculation different growth, related parameters were investigated. Collected data on growth related parameter after rhizobia inoculation were analyzed using SAS software version 9.1. Rhizobial treatment effect on plant growth related parameters were investigated. Effect of treatment was significant at (PPhaseolus Vulgaris L.) study on field trial should be future perspective of the area.
},
year = {2025}
}
TY - JOUR T1 - Isolation, Characterization and Evaluation of Symbiotic Effectiveness of Rhizobia Nodulating (Phaseolus Vulgaris L) Nodules Collected from Gurafarda, Southwest Ethiopia AU - Andualem Arimo Turito AU - Israel Zewde AU - Nigatu Ebisa Nemomsa AU - Adugna Abdissa Belew Y1 - 2025/05/19 PY - 2025 N1 - https://doi.org/10.11648/j.ijmb.20251002.12 DO - 10.11648/j.ijmb.20251002.12 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 39 EP - 53 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20251002.12 AB - Rhizobia are diazotrophic bacteria that fix nitrogen after becoming established inside the root nodules of legumes (Fabaceae). This study was conducted to isolate, characterize and evaluate the symbiotic effectiveness of rhizobia nodulating common bean. Nodule sample were collected from six randomly selected kebele of the study area for nodule induction under controlled condition. At the flowering stage after 45 days of plant growth, root nodules were collected and rhizobia bacteria isolated. The isolates were allowed for different biochemical and physiological characterization. Presumptive tests confirmed that isolates were root nodulating rhizobia. Rhizobial inoculants (Bio-fertilizer) effect on the host crop was tested under greenhouse condition on sterilized river sand culture. Plant treated with KNO3 were set as positive and plants treated with sterile distilled water were used as negative control. Isolates treated plant group were experimental group. The experiment was laid in CRD design. After 45 days of inoculation different growth, related parameters were investigated. Collected data on growth related parameter after rhizobia inoculation were analyzed using SAS software version 9.1. Rhizobial treatment effect on plant growth related parameters were investigated. Effect of treatment was significant at (PPhaseolus Vulgaris L.) study on field trial should be future perspective of the area. VL - 10 IS - 2 ER -
Department of Biology, Mizan Tepi University, Tepi, Ethiopia
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