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Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI

Received: 9 October 2021     Accepted: 25 October 2021     Published: 10 November 2021
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Abstract

Background: Urinary tract infection (UTI) is among the most common bacterial infections and possess significant healthcare burden. Hence a study was necessary to apply PCR technology directly to clinical specimens to allow early and accurate identification of pathogens of UTI. Objective: Diagnosis of major uropathogens by different laboratory diagnostic procedures including polymerase chain reaction (PCR) directly from urine specimens from suspected patients of urinary tract infection. Methods: This study was carried out in the department of Microbiology, Mymensingh Medical College during the period from July 2016 to June 2017. Urine specimens were collected and isolation and identification of major uropathogens (Escherichiacoli Klehsiella pneumonias, Proteusmirabilis, and Pseudomonas aeruginosa) were done by standard microbiological procedure anbiochemical tests. PCR was performed by using standard protocol with species specific primer for detection offim H gene for Escherichia coli, fimK gene for Klehsiella paeunomiae, UreC for Proteus mirabilis and ETA for Pseudomonas aeruginasa. Results: Out of 250 urine specimens, 200 specimens were isolated and identified by culture and different biochemical methods which were supported by microscopical examination and at the same time PCR could detect species specific genes in 201 specimens directly from urine of suspected UTI patient Escherichia coli was responsible as a leading causative pathogen in both outpatient department and in patient department. Urine specimens was higher in female in both out patient population and inpatient population. Culture positivity of in patient population among the male (45.5%) was slighty higher than that of outpatient population (34.5%). The predominant age group suffered from UTI in case of outpatient population was >15-30 but for the in patient population, the age group was 60 years and above. 90 culture positive in patient population, 43 (95.5%) were from the gynae unit, 23 (92%) were from surgery unit, 8 (72.7%) were from the medicine unit, 12 (85.7%) were from orthopaedic unit and 4 (80%) were from paediatric unit. On the other hand Pseudomonas aeruginosa Profeus mirabilis and Klebsiella pneumonia were more prevalent in in-patient department and it was 21.1%, 5.6% and 5.5% respectively, Among the 50 culture negative urine specimens, 14 (28%) showed PCR positive for Escherichia coli, Klehsiella pneumonia and Pseudomonas aeruginosa. Conclusion: This study revealed that, the prevalence of UTI is high n MMCH Single pathogen base uniplex PCR was found superior than standard culture and less time consuming. Because uniplex PCR could detect many (28%) culture negative cases.

Published in International Journal of Microbiology and Biotechnology (Volume 6, Issue 4)
DOI 10.11648/j.ijmb.20210604.13
Page(s) 119-124
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Major Uropathogens, Different Laboratory Diagnostic, Polymerase Chain Reaction (PCR)

References
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    Mahbuba Sultana, Akram Hossain, Shyamal Kumar Paul, Nobumichi Kobayashi, Md. Sharif Hossain, et al. (2021). Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI. International Journal of Microbiology and Biotechnology, 6(4), 119-124. https://doi.org/10.11648/j.ijmb.20210604.13

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    Mahbuba Sultana; Akram Hossain; Shyamal Kumar Paul; Nobumichi Kobayashi; Md. Sharif Hossain, et al. Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI. Int. J. Microbiol. Biotechnol. 2021, 6(4), 119-124. doi: 10.11648/j.ijmb.20210604.13

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    AMA Style

    Mahbuba Sultana, Akram Hossain, Shyamal Kumar Paul, Nobumichi Kobayashi, Md. Sharif Hossain, et al. Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI. Int J Microbiol Biotechnol. 2021;6(4):119-124. doi: 10.11648/j.ijmb.20210604.13

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  • @article{10.11648/j.ijmb.20210604.13,
      author = {Mahbuba Sultana and Akram Hossain and Shyamal Kumar Paul and Nobumichi Kobayashi and Md. Sharif Hossain and Mst. Nazma khatun and Mahbubul Alam},
      title = {Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {6},
      number = {4},
      pages = {119-124},
      doi = {10.11648/j.ijmb.20210604.13},
      url = {https://doi.org/10.11648/j.ijmb.20210604.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20210604.13},
      abstract = {Background: Urinary tract infection (UTI) is among the most common bacterial infections and possess significant healthcare burden. Hence a study was necessary to apply PCR technology directly to clinical specimens to allow early and accurate identification of pathogens of UTI. Objective: Diagnosis of major uropathogens by different laboratory diagnostic procedures including polymerase chain reaction (PCR) directly from urine specimens from suspected patients of urinary tract infection. Methods: This study was carried out in the department of Microbiology, Mymensingh Medical College during the period from July 2016 to June 2017. Urine specimens were collected and isolation and identification of major uropathogens (Escherichiacoli Klehsiella pneumonias, Proteusmirabilis, and Pseudomonas aeruginosa) were done by standard microbiological procedure anbiochemical tests. PCR was performed by using standard protocol with species specific primer for detection offim H gene for Escherichia coli, fimK gene for Klehsiella paeunomiae, UreC for Proteus mirabilis and ETA for Pseudomonas aeruginasa. Results: Out of 250 urine specimens, 200 specimens were isolated and identified by culture and different biochemical methods which were supported by microscopical examination and at the same time PCR could detect species specific genes in 201 specimens directly from urine of suspected UTI patient Escherichia coli was responsible as a leading causative pathogen in both outpatient department and in patient department. Urine specimens was higher in female in both out patient population and inpatient population. Culture positivity of in patient population among the male (45.5%) was slighty higher than that of outpatient population (34.5%). The predominant age group suffered from UTI in case of outpatient population was >15-30 but for the in patient population, the age group was 60 years and above. 90 culture positive in patient population, 43 (95.5%) were from the gynae unit, 23 (92%) were from surgery unit, 8 (72.7%) were from the medicine unit, 12 (85.7%) were from orthopaedic unit and 4 (80%) were from paediatric unit. On the other hand Pseudomonas aeruginosa Profeus mirabilis and Klebsiella pneumonia were more prevalent in in-patient department and it was 21.1%, 5.6% and 5.5% respectively, Among the 50 culture negative urine specimens, 14 (28%) showed PCR positive for Escherichia coli, Klehsiella pneumonia and Pseudomonas aeruginosa. Conclusion: This study revealed that, the prevalence of UTI is high n MMCH Single pathogen base uniplex PCR was found superior than standard culture and less time consuming. Because uniplex PCR could detect many (28%) culture negative cases.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI
    AU  - Mahbuba Sultana
    AU  - Akram Hossain
    AU  - Shyamal Kumar Paul
    AU  - Nobumichi Kobayashi
    AU  - Md. Sharif Hossain
    AU  - Mst. Nazma khatun
    AU  - Mahbubul Alam
    Y1  - 2021/11/10
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ijmb.20210604.13
    DO  - 10.11648/j.ijmb.20210604.13
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 119
    EP  - 124
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20210604.13
    AB  - Background: Urinary tract infection (UTI) is among the most common bacterial infections and possess significant healthcare burden. Hence a study was necessary to apply PCR technology directly to clinical specimens to allow early and accurate identification of pathogens of UTI. Objective: Diagnosis of major uropathogens by different laboratory diagnostic procedures including polymerase chain reaction (PCR) directly from urine specimens from suspected patients of urinary tract infection. Methods: This study was carried out in the department of Microbiology, Mymensingh Medical College during the period from July 2016 to June 2017. Urine specimens were collected and isolation and identification of major uropathogens (Escherichiacoli Klehsiella pneumonias, Proteusmirabilis, and Pseudomonas aeruginosa) were done by standard microbiological procedure anbiochemical tests. PCR was performed by using standard protocol with species specific primer for detection offim H gene for Escherichia coli, fimK gene for Klehsiella paeunomiae, UreC for Proteus mirabilis and ETA for Pseudomonas aeruginasa. Results: Out of 250 urine specimens, 200 specimens were isolated and identified by culture and different biochemical methods which were supported by microscopical examination and at the same time PCR could detect species specific genes in 201 specimens directly from urine of suspected UTI patient Escherichia coli was responsible as a leading causative pathogen in both outpatient department and in patient department. Urine specimens was higher in female in both out patient population and inpatient population. Culture positivity of in patient population among the male (45.5%) was slighty higher than that of outpatient population (34.5%). The predominant age group suffered from UTI in case of outpatient population was >15-30 but for the in patient population, the age group was 60 years and above. 90 culture positive in patient population, 43 (95.5%) were from the gynae unit, 23 (92%) were from surgery unit, 8 (72.7%) were from the medicine unit, 12 (85.7%) were from orthopaedic unit and 4 (80%) were from paediatric unit. On the other hand Pseudomonas aeruginosa Profeus mirabilis and Klebsiella pneumonia were more prevalent in in-patient department and it was 21.1%, 5.6% and 5.5% respectively, Among the 50 culture negative urine specimens, 14 (28%) showed PCR positive for Escherichia coli, Klehsiella pneumonia and Pseudomonas aeruginosa. Conclusion: This study revealed that, the prevalence of UTI is high n MMCH Single pathogen base uniplex PCR was found superior than standard culture and less time consuming. Because uniplex PCR could detect many (28%) culture negative cases.
    VL  - 6
    IS  - 4
    ER  - 

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Author Information
  • Department of Microbiology, Shaheed Tajuddin Ahmed Medical College, Gazipur, Bangladesh

  • Microbiology, Department of Laboratory Medicine, Imperial Hospital, Chattogram, Bangladesh

  • Microbiology, Netrokona Medical College, Netrokona, Bangladesh

  • Department of Hygiene, Sapporo Medical University, School of Medicine, Sapporo, Japan

  • Department of Orthopedics, Shaheed Tajuddin Ahmed Medical College Hospital, Gazipur, Bangladesh

  • Department of Microbiology, Shaheed Tajuddin Ahmed Medical College, Gazipur, Bangladesh

  • Department of Microbiology, Shaheed Tajuddin Ahmed Medical College, Gazipur, Bangladesh

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