| Peer-Reviewed

Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State

Received: 26 January 2020     Accepted: 12 February 2020     Published: 19 February 2020
Views:       Downloads:
Abstract

The spillage of oil and other petroleum products have been a major source of environmental pollution globally. Rampant use of chemical remediation methods were shown to be noxious with detrimental consequences. Exploration of microbiodata for probable candidate to be utilized for degradation of oil spillage or contamination is of great interest. In this study, soil samples from 10 different mechanic workshops in mechanic village Dutse were collected as well as control sample from unpolluted hydrocarbon free soil at same site, bacterial species were isolated, identified using biochemical and molecular 16S rRNA gene sequencing and post sequencing analyses. Species enumerated were Bacillus cereaus, Bacillus subtilis, Bacillus licheniformis and Paenibacillus alvei, The sequences were compared to closest relative species in the GenBank data base of National Centre for Biotechnology Information. However, the substrate used was engine oil and all the isolates were found to utilized the test substrate. Biodegradation studies have been carried out through Gas Chromatography Mass Spectrometry (GC-MS). Of all the isolates, Bacillus subtilis might be the most hydrocarbon degrading bacterial species due to high average relative abundance concentration of degraded hydrocarbon compound.

Published in International Journal of Microbiology and Biotechnology (Volume 5, Issue 1)
DOI 10.11648/j.ijmb.20200501.15
Page(s) 28-33
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Bacteria, Bioremediation, Hydrocarbon, Oil Spill, Petroleum, Pollution, Polymerase Chain Reaction (PCR)

References
[1] Abdulsalam, S., Adefila, S. S., Bugaje, I. M. and Ibrahim, S. C (2012). Bioremediation of soil contaminated with used motor oil in a closed system. J. Bioremediation and Biodegradation 3 (12): 1-7.
[2] Alexander M. (2004). Biodegradation and Bioremediation. San Diego: Academic press, PP 43-48.
[3] Balba M. T., Al-Awadhi N, Al-Daher R. (2008). Bioremedition of oil- contaminated soil; Microbiological Methods for feasibility assessment and field evaluation. J. Microbiol. Methods, 32: 155-164.
[4] Benson, H. J. (1994). Microbiological Applications seventh edition; Laboratory Manual in General Microbiology. McGraw-Hill 22: 26-31.
[5] Binta L. M (2014). Studies on the Hydrocarbon-utilization Potentials of Bacteria and Fungi Isolated from Soil Contaminated with Petroleum Products in Kano, Nigeria. Pp 22-43.
[6] Chailan, F., Le Fleche, A., Bury, E., Phantavog, Y. H., Grimont, P., Saliot, A. and Oudot, J. (2004). Identification and biodegradation potential of tropical aerobic hydrocarbon degrading microorganisms. Research in microbiology.
[7] Eja, M. E., Udo S. M and Asikong, B. E (2005). Bioremediation Potential of Bacillus species in oil polluted soil from automechanic workshops in Calabar, Nigeria, Africa J. Environ. Pullut. Health 2 (1): 11-18.
[8] Fatiha D., Abdelwahed C., Khadidja S. R. (2017). Isolation and Molecular Identification of hydrocarbon degrading bacteria from oil contaminated soil. Internl. Journal of Bioscience. ISSN: 2220-6655. Vol. 11, No. 4, p. 272-278.
[9] Fatima M., Flavio A., Benedict C., Williams T. (2005). Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. Bioresource Tech., 96: 1049-1055.
[10] Hamme, J. D., Van A., Singh, O. P. (2003) Microbiol. Mol Biol. Rev. 67: 503.
[11] Howsam, M. and Jones, K. C (2008), “sources of PAHs in the environment. In: Neilson AH (ed). Anthropogenic compounds. PAHs and related compounds” springer, Berlin, Germany, pp 137-174.
[12] Ismaila, S. (2008): Bioremediation laboratory manual. Department of Biotechnology, Islamic University, Gaza, Israel pp 971-999.
[13] Leahy J. G, Colwell R. R (2000). Microbial degradation of hydrocarbons in the environment. Microbial. Rev, 54: 305-315.
[14] Less Z. M., Senior, E. (1995). Bioremediation. A practical solution to land pollution. In: Clean Technology and the Environment chapman and Hall, New York. 121-146 (26 years).
[15] NITTTR, Chandigarh Technology Enabled Learning (NCTEL). Dec., 2013. Determination of water content of soil by oven drying method- youtube.
[16] Okoh, A. I and M. R. Trejo-Hermandez, (2006). Remediation of petroleum hydrocarbon polluted systems: exploiting the bioremediation strategies. African J. Biotechnol, 5: 2520-2525.
[17] Olakunle, F. (2013). Characterization of indigenious microorganisms associated with crude oil polluted soil and water. Microbiology journal 56: 2567-2569.
[18] Ritesh K. N. (2012). Determination of organic carbon of soil. Made with KINEMASTER-youtube.
[19] Scherrer P., Mille G. (1990). Biodegradation of crude oil in experimentally-polluted clayey and sandy mangrove soils. Oil and Chemical Pollution 6, 163-176.
[20] Sims, R. C and Overcash, M. R (2014), “Fate of polynuclear aromatic compounds (PNAs) in soil-plant system” Res Rev., 88, pp 1-67.
[21] Soriano M. and Pereira, D. C. (1998). Nutrient removal from aquaculture wastewater using macroalgae Garcilaria birdiae. Biomass and Bioenergy 33: 327-331.
[22] Throne-Host, M., Wentzel, A., Ellingsten, T. E., Kotlar, H. K. and zotcher, S. B. (2007). Identification of novel genes involved in long-chain n-alkane degradation by Acinetobacteria sp. Applied and environmental microbiology.
[23] United States Environment Protection Agency (USEPA) (2015): Characteristics of spilled oils, fuels and Petroleum products; composition and properties. May, 2015.
[24] Vidali M (2010), “Bioremediation; An overview;” pure Appl. Chem., 73 (7), pp 1163-1172.
[25] Widdle F. and Rabus R. (2001). Anaerobic Biodegradation of Saturated and Aromatic hydrocarbons. Curr. Opin. Biotechnol., 12: 259-276.
[26] Yakimov, M. M., Timmis, K. N and Golyshin, P. N. (2007). Obligate oil degrading marine bacteria. Current opinions in Biotechnology.
Cite This Article
  • APA Style

    Ringim Mahi Saidu, Gumel Ahmad Mohammed, Shiaka Gimba Peter. (2020). Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State. International Journal of Microbiology and Biotechnology, 5(1), 28-33. https://doi.org/10.11648/j.ijmb.20200501.15

    Copy | Download

    ACS Style

    Ringim Mahi Saidu; Gumel Ahmad Mohammed; Shiaka Gimba Peter. Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State. Int. J. Microbiol. Biotechnol. 2020, 5(1), 28-33. doi: 10.11648/j.ijmb.20200501.15

    Copy | Download

    AMA Style

    Ringim Mahi Saidu, Gumel Ahmad Mohammed, Shiaka Gimba Peter. Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State. Int J Microbiol Biotechnol. 2020;5(1):28-33. doi: 10.11648/j.ijmb.20200501.15

    Copy | Download

  • @article{10.11648/j.ijmb.20200501.15,
      author = {Ringim Mahi Saidu and Gumel Ahmad Mohammed and Shiaka Gimba Peter},
      title = {Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {5},
      number = {1},
      pages = {28-33},
      doi = {10.11648/j.ijmb.20200501.15},
      url = {https://doi.org/10.11648/j.ijmb.20200501.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200501.15},
      abstract = {The spillage of oil and other petroleum products have been a major source of environmental pollution globally. Rampant use of chemical remediation methods were shown to be noxious with detrimental consequences. Exploration of microbiodata for probable candidate to be utilized for degradation of oil spillage or contamination is of great interest. In this study, soil samples from 10 different mechanic workshops in mechanic village Dutse were collected as well as control sample from unpolluted hydrocarbon free soil at same site, bacterial species were isolated, identified using biochemical and molecular 16S rRNA gene sequencing and post sequencing analyses. Species enumerated were Bacillus cereaus, Bacillus subtilis, Bacillus licheniformis and Paenibacillus alvei, The sequences were compared to closest relative species in the GenBank data base of National Centre for Biotechnology Information. However, the substrate used was engine oil and all the isolates were found to utilized the test substrate. Biodegradation studies have been carried out through Gas Chromatography Mass Spectrometry (GC-MS). Of all the isolates, Bacillus subtilis might be the most hydrocarbon degrading bacterial species due to high average relative abundance concentration of degraded hydrocarbon compound.},
     year = {2020}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State
    AU  - Ringim Mahi Saidu
    AU  - Gumel Ahmad Mohammed
    AU  - Shiaka Gimba Peter
    Y1  - 2020/02/19
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijmb.20200501.15
    DO  - 10.11648/j.ijmb.20200501.15
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 28
    EP  - 33
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20200501.15
    AB  - The spillage of oil and other petroleum products have been a major source of environmental pollution globally. Rampant use of chemical remediation methods were shown to be noxious with detrimental consequences. Exploration of microbiodata for probable candidate to be utilized for degradation of oil spillage or contamination is of great interest. In this study, soil samples from 10 different mechanic workshops in mechanic village Dutse were collected as well as control sample from unpolluted hydrocarbon free soil at same site, bacterial species were isolated, identified using biochemical and molecular 16S rRNA gene sequencing and post sequencing analyses. Species enumerated were Bacillus cereaus, Bacillus subtilis, Bacillus licheniformis and Paenibacillus alvei, The sequences were compared to closest relative species in the GenBank data base of National Centre for Biotechnology Information. However, the substrate used was engine oil and all the isolates were found to utilized the test substrate. Biodegradation studies have been carried out through Gas Chromatography Mass Spectrometry (GC-MS). Of all the isolates, Bacillus subtilis might be the most hydrocarbon degrading bacterial species due to high average relative abundance concentration of degraded hydrocarbon compound.
    VL  - 5
    IS  - 1
    ER  - 

    Copy | Download

Author Information
  • Department of Microbiology and Biotechnology, Faculty of science, Federal University Dutse, Dutse City, Jigawa State, Nigeria

  • Department of Microbiology and Biotechnology, Faculty of science, Federal University Dutse, Dutse City, Jigawa State, Nigeria

  • Department of Microbiology and Biotechnology, Faculty of science, Federal University Dutse, Dutse City, Jigawa State, Nigeria

  • Sections