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Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar

Received: 11 September 2018     Accepted: 21 September 2018     Published: 30 October 2018
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Abstract

Heavy metals contamination is now widespread in the nature. At higher concentration, heavy metals become toxic and disturb the ecosystem including soil microorganisms. To adapt to such constraints, some microorganisms have developed tolerance mechanisms. Indeed, in the environment, the resistance of microorganisms to heavy metal often promotes to antibiotic resistance. This work aims to isolate strains from soil samples collected in Andralanitra landfill, to test their tolerance to heavy metals, to identify tolerant strains and to verify their resistance to antibiotics. According to the dilution method, a total of 48 strains were obtained, 14 were isolated on PDA medium, 10 on Sabouraud agar medium, 10 strains on Mossel agar medium, 7 on AS1 medium, 5 strains on TSA medium and 2 strains with King B medium. Resistance test to heavy metals performed by the wells method showed that out of the 48 isolated strains, 26 were capable to grow in the presence of heavy metals (solution composed of copper, zinc, cadmium, chromium, nickel, lead) at different concentrations. The highest number of tolerant strains was recorded at the concentration of 100mg/L ≤ C ≤ 1000mg/L. Four (4) strains were tolerant to the heavy metals solution at a concentration between 100mg/L and 1500mg/L. The molecular identification of these four most resistant strains by 16S rDNA gene sequencing and ITS gene sequencing allowed to classify them as belonging to the genera Ochrobactrum pseudogrignonense, Arthrobacter nicotianae, Penicillium crustosum and Penicillium commune. The antibiotic sensitivity test using disc diffusion method on Mueller-Hinton agar revealed that Ochrobactrum pseudogrignonense and Penicillium commune were resistant to Trimethoprim, Arthrobacter nicotianae showed resistance to Trimethoprim and Ciprofloxacin, Penicillium crustosum was resistant to all tested antibiotics.

Published in International Journal of Microbiology and Biotechnology (Volume 3, Issue 3)
DOI 10.11648/j.ijmb.20180303.12
Page(s) 71-78
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Isolation, Characterization, Resistance, Heavy Metals, Antibiotics, Dumpsite, MADAGASCAR

References
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    Hanitrinisoa Harimisa Andriamafana, Yves Mong, Onja Andriambeloson, Christine Ravonizafy, Marson Raherimandimby, et al. (2018). Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar. International Journal of Microbiology and Biotechnology, 3(3), 71-78. https://doi.org/10.11648/j.ijmb.20180303.12

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    ACS Style

    Hanitrinisoa Harimisa Andriamafana; Yves Mong; Onja Andriambeloson; Christine Ravonizafy; Marson Raherimandimby, et al. Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar. Int. J. Microbiol. Biotechnol. 2018, 3(3), 71-78. doi: 10.11648/j.ijmb.20180303.12

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    AMA Style

    Hanitrinisoa Harimisa Andriamafana, Yves Mong, Onja Andriambeloson, Christine Ravonizafy, Marson Raherimandimby, et al. Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar. Int J Microbiol Biotechnol. 2018;3(3):71-78. doi: 10.11648/j.ijmb.20180303.12

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  • @article{10.11648/j.ijmb.20180303.12,
      author = {Hanitrinisoa Harimisa Andriamafana and Yves Mong and Onja Andriambeloson and Christine Ravonizafy and Marson Raherimandimby and Rado Rasolomampianina},
      title = {Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {3},
      number = {3},
      pages = {71-78},
      doi = {10.11648/j.ijmb.20180303.12},
      url = {https://doi.org/10.11648/j.ijmb.20180303.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20180303.12},
      abstract = {Heavy metals contamination is now widespread in the nature. At higher concentration, heavy metals become toxic and disturb the ecosystem including soil microorganisms. To adapt to such constraints, some microorganisms have developed tolerance mechanisms. Indeed, in the environment, the resistance of microorganisms to heavy metal often promotes to antibiotic resistance. This work aims to isolate strains from soil samples collected in Andralanitra landfill, to test their tolerance to heavy metals, to identify tolerant strains and to verify their resistance to antibiotics. According to the dilution method, a total of 48 strains were obtained, 14 were isolated on PDA medium, 10 on Sabouraud agar medium, 10 strains on Mossel agar medium, 7 on AS1 medium, 5 strains on TSA medium and 2 strains with King B medium. Resistance test to heavy metals performed by the wells method showed that out of the 48 isolated strains, 26 were capable to grow in the presence of heavy metals (solution composed of copper, zinc, cadmium, chromium, nickel, lead) at different concentrations. The highest number of tolerant strains was recorded at the concentration of 100mg/L ≤ C ≤ 1000mg/L. Four (4) strains were tolerant to the heavy metals solution at a concentration between 100mg/L and 1500mg/L. The molecular identification of these four most resistant strains by 16S rDNA gene sequencing and ITS gene sequencing allowed to classify them as belonging to the genera Ochrobactrum pseudogrignonense, Arthrobacter nicotianae, Penicillium crustosum and Penicillium commune. The antibiotic sensitivity test using disc diffusion method on Mueller-Hinton agar revealed that Ochrobactrum pseudogrignonense and Penicillium commune were resistant to Trimethoprim, Arthrobacter nicotianae showed resistance to Trimethoprim and Ciprofloxacin, Penicillium crustosum was resistant to all tested antibiotics.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar
    AU  - Hanitrinisoa Harimisa Andriamafana
    AU  - Yves Mong
    AU  - Onja Andriambeloson
    AU  - Christine Ravonizafy
    AU  - Marson Raherimandimby
    AU  - Rado Rasolomampianina
    Y1  - 2018/10/30
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ijmb.20180303.12
    DO  - 10.11648/j.ijmb.20180303.12
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 71
    EP  - 78
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20180303.12
    AB  - Heavy metals contamination is now widespread in the nature. At higher concentration, heavy metals become toxic and disturb the ecosystem including soil microorganisms. To adapt to such constraints, some microorganisms have developed tolerance mechanisms. Indeed, in the environment, the resistance of microorganisms to heavy metal often promotes to antibiotic resistance. This work aims to isolate strains from soil samples collected in Andralanitra landfill, to test their tolerance to heavy metals, to identify tolerant strains and to verify their resistance to antibiotics. According to the dilution method, a total of 48 strains were obtained, 14 were isolated on PDA medium, 10 on Sabouraud agar medium, 10 strains on Mossel agar medium, 7 on AS1 medium, 5 strains on TSA medium and 2 strains with King B medium. Resistance test to heavy metals performed by the wells method showed that out of the 48 isolated strains, 26 were capable to grow in the presence of heavy metals (solution composed of copper, zinc, cadmium, chromium, nickel, lead) at different concentrations. The highest number of tolerant strains was recorded at the concentration of 100mg/L ≤ C ≤ 1000mg/L. Four (4) strains were tolerant to the heavy metals solution at a concentration between 100mg/L and 1500mg/L. The molecular identification of these four most resistant strains by 16S rDNA gene sequencing and ITS gene sequencing allowed to classify them as belonging to the genera Ochrobactrum pseudogrignonense, Arthrobacter nicotianae, Penicillium crustosum and Penicillium commune. The antibiotic sensitivity test using disc diffusion method on Mueller-Hinton agar revealed that Ochrobactrum pseudogrignonense and Penicillium commune were resistant to Trimethoprim, Arthrobacter nicotianae showed resistance to Trimethoprim and Ciprofloxacin, Penicillium crustosum was resistant to all tested antibiotics.
    VL  - 3
    IS  - 3
    ER  - 

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Author Information
  • Department of Fundamental and Applied Biochemistry, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar

  • National Center of Environmental Research, Antananarivo, Madagascar

  • National Center of Environmental Research, Antananarivo, Madagascar

  • National Center of Environmental Research, Antananarivo, Madagascar

  • Department of Fundamental and Applied Biochemistry, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar

  • National Center of Environmental Research, Antananarivo, Madagascar

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