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Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal

Received: 17 January 2018     Accepted: 19 March 2018     Published: 10 April 2018
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Abstract

With the objective to isolate, identify and determine the antimicrobial activity of the soil actinomycetes, this study was done in the laboratory of Nepal Polytechnic Institute, Chitwan (September 2015- April 2016). A total of 30 soil samples were randomly collected and processed from forest, garden, cultivated lands and river side (Narayani and Rapti River) of Chitwan, Nepal. Isolates were identified by colony characterizations, microscopy and biochemical tests. Primary and secondary screening for antibiotic producers was done by single line cross streak and agar cup assay respectively against test organisms, S. Typhi, S. aureus and E. coli. Altogether 25 different actinomycetes from two genera viz; Streptomyces (n=15) and Micromonospora (n=10) were identified from selected sites (n=10; river side, n=5 each from garden, cultivated and forest soil). Primary screening of n=12 isolates was done by inhibition to at least one test bacterium. Of them only n=9 (n=6 Streptomyces spp., n=3 Micromonospora spp.) isolates showed promising antibacterial activity (zone of inhibition above 8mm) on secondary screening. Maximum zone of inhibition was 14mm observed against S. aureus and whereas 18mm against E. coli and S. Typhi respectively. This finding indicates potent antibiotic producer actinomycetes were isolated from soil of Chitwan, study furthering strain selection and identification would pave the way for commercial antibiotic production.

Published in International Journal of Microbiology and Biotechnology (Volume 3, Issue 1)
DOI 10.11648/j.ijmb.20180301.14
Page(s) 25-30
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Actinomycetes, Chitwan, Antibacterial

References
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  • APA Style

    Kamal Rai, Sujan Khadka, Bidya Shrestha. (2018). Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal. International Journal of Microbiology and Biotechnology, 3(1), 25-30. https://doi.org/10.11648/j.ijmb.20180301.14

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    ACS Style

    Kamal Rai; Sujan Khadka; Bidya Shrestha. Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal. Int. J. Microbiol. Biotechnol. 2018, 3(1), 25-30. doi: 10.11648/j.ijmb.20180301.14

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    AMA Style

    Kamal Rai, Sujan Khadka, Bidya Shrestha. Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal. Int J Microbiol Biotechnol. 2018;3(1):25-30. doi: 10.11648/j.ijmb.20180301.14

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  • @article{10.11648/j.ijmb.20180301.14,
      author = {Kamal Rai and Sujan Khadka and Bidya Shrestha},
      title = {Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {3},
      number = {1},
      pages = {25-30},
      doi = {10.11648/j.ijmb.20180301.14},
      url = {https://doi.org/10.11648/j.ijmb.20180301.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20180301.14},
      abstract = {With the objective to isolate, identify and determine the antimicrobial activity of the soil actinomycetes, this study was done in the laboratory of Nepal Polytechnic Institute, Chitwan (September 2015- April 2016). A total of 30 soil samples were randomly collected and processed from forest, garden, cultivated lands and river side (Narayani and Rapti River) of Chitwan, Nepal. Isolates were identified by colony characterizations, microscopy and biochemical tests. Primary and secondary screening for antibiotic producers was done by single line cross streak and agar cup assay respectively against test organisms, S. Typhi, S. aureus and E. coli. Altogether 25 different actinomycetes from two genera viz; Streptomyces (n=15) and Micromonospora (n=10) were identified from selected sites (n=10; river side, n=5 each from garden, cultivated and forest soil). Primary screening of n=12 isolates was done by inhibition to at least one test bacterium. Of them only n=9 (n=6 Streptomyces spp., n=3 Micromonospora spp.) isolates showed promising antibacterial activity (zone of inhibition above 8mm) on secondary screening. Maximum zone of inhibition was 14mm observed against S. aureus and whereas 18mm against E. coli and S. Typhi respectively. This finding indicates potent antibiotic producer actinomycetes were isolated from soil of Chitwan, study furthering strain selection and identification would pave the way for commercial antibiotic production.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal
    AU  - Kamal Rai
    AU  - Sujan Khadka
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    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
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    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20180301.14
    AB  - With the objective to isolate, identify and determine the antimicrobial activity of the soil actinomycetes, this study was done in the laboratory of Nepal Polytechnic Institute, Chitwan (September 2015- April 2016). A total of 30 soil samples were randomly collected and processed from forest, garden, cultivated lands and river side (Narayani and Rapti River) of Chitwan, Nepal. Isolates were identified by colony characterizations, microscopy and biochemical tests. Primary and secondary screening for antibiotic producers was done by single line cross streak and agar cup assay respectively against test organisms, S. Typhi, S. aureus and E. coli. Altogether 25 different actinomycetes from two genera viz; Streptomyces (n=15) and Micromonospora (n=10) were identified from selected sites (n=10; river side, n=5 each from garden, cultivated and forest soil). Primary screening of n=12 isolates was done by inhibition to at least one test bacterium. Of them only n=9 (n=6 Streptomyces spp., n=3 Micromonospora spp.) isolates showed promising antibacterial activity (zone of inhibition above 8mm) on secondary screening. Maximum zone of inhibition was 14mm observed against S. aureus and whereas 18mm against E. coli and S. Typhi respectively. This finding indicates potent antibiotic producer actinomycetes were isolated from soil of Chitwan, study furthering strain selection and identification would pave the way for commercial antibiotic production.
    VL  - 3
    IS  - 1
    ER  - 

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Author Information
  • Department of Biological Protein Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, P. R. China

  • Department of Life Sciences, Biochemistry and Molecular Biology, Central China Normal University, Wuhan, P. R. China

  • Department of Microbiology, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal

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