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Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice

Received: 27 October 2021     Accepted: 22 November 2021     Published: 2 December 2021
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Abstract

Recent studies have demonstrated that pain is partially regulated by the gut microbiota. However, the association of gut microbiota with painful diabetic neuropathy, a common complication of diabetes, remains unclear. Herein, we investigated whether the gut microbiota is associated with mechanical allodynia during the early stage of hyperglycemia in 7-week-old db/db mice. The db/db mice were intraperitoneally injected with metformin for 2 weeks. Using the von Frey test and gut microbiota analyses, we investigated the association of gut microbial changes with mechanical allodynia in 7-week-old mice. In db/db mice, colonic microbial community profiles were altered, and both unweighted and weighted UniFrac distances were reduced. Colonic genus-level abundances of Alloprevotella and Prevotellaceae_UCG-001 were positively correlated with mechanical allodynia in db/db mice, while the abundance of Odoribacter was negatively correlated. Intraperitoneal injection of metformin for 2 weeks alleviated mechanical allodynia in db/db mice but did not achieve an anti-diabetic effect. Metformin altered colonic microbial communities and increased weighted UniFrac distance in db/db mice, although its analgesic effect was not associated with specific bacteria. Additionally, alteration of small intestinal microbial community profiles and reduction in weighted UniFrac distance were observed in db/db mice, which were not affected by metformin. These results provide potential evidence of the association of the gut microbiota with mechanical allodynia during early-stage of hyperglycemia.

Published in International Journal of Diabetes and Endocrinology (Volume 6, Issue 4)
DOI 10.11648/j.ijde.20210604.15
Page(s) 150-159
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Gut Microbiota, Painful Diabetic Neuropathy, Mechanical Allodynia, Metformin

References
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Cite This Article
  • APA Style

    Xinting Wang, Jihong Wu, Simin Qi, Rui Huang, Xiaoping Wu, et al. (2021). Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice. International Journal of Diabetes and Endocrinology, 6(4), 150-159. https://doi.org/10.11648/j.ijde.20210604.15

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    ACS Style

    Xinting Wang; Jihong Wu; Simin Qi; Rui Huang; Xiaoping Wu, et al. Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice. Int. J. Diabetes Endocrinol. 2021, 6(4), 150-159. doi: 10.11648/j.ijde.20210604.15

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    AMA Style

    Xinting Wang, Jihong Wu, Simin Qi, Rui Huang, Xiaoping Wu, et al. Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice. Int J Diabetes Endocrinol. 2021;6(4):150-159. doi: 10.11648/j.ijde.20210604.15

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  • @article{10.11648/j.ijde.20210604.15,
      author = {Xinting Wang and Jihong Wu and Simin Qi and Rui Huang and Xiaoping Wu and Lin Tian and Peili Wang and Shenglan Wang},
      title = {Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice},
      journal = {International Journal of Diabetes and Endocrinology},
      volume = {6},
      number = {4},
      pages = {150-159},
      doi = {10.11648/j.ijde.20210604.15},
      url = {https://doi.org/10.11648/j.ijde.20210604.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijde.20210604.15},
      abstract = {Recent studies have demonstrated that pain is partially regulated by the gut microbiota. However, the association of gut microbiota with painful diabetic neuropathy, a common complication of diabetes, remains unclear. Herein, we investigated whether the gut microbiota is associated with mechanical allodynia during the early stage of hyperglycemia in 7-week-old db/db mice. The db/db mice were intraperitoneally injected with metformin for 2 weeks. Using the von Frey test and gut microbiota analyses, we investigated the association of gut microbial changes with mechanical allodynia in 7-week-old mice. In db/db mice, colonic microbial community profiles were altered, and both unweighted and weighted UniFrac distances were reduced. Colonic genus-level abundances of Alloprevotella and Prevotellaceae_UCG-001 were positively correlated with mechanical allodynia in db/db mice, while the abundance of Odoribacter was negatively correlated. Intraperitoneal injection of metformin for 2 weeks alleviated mechanical allodynia in db/db mice but did not achieve an anti-diabetic effect. Metformin altered colonic microbial communities and increased weighted UniFrac distance in db/db mice, although its analgesic effect was not associated with specific bacteria. Additionally, alteration of small intestinal microbial community profiles and reduction in weighted UniFrac distance were observed in db/db mice, which were not affected by metformin. These results provide potential evidence of the association of the gut microbiota with mechanical allodynia during early-stage of hyperglycemia.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice
    AU  - Xinting Wang
    AU  - Jihong Wu
    AU  - Simin Qi
    AU  - Rui Huang
    AU  - Xiaoping Wu
    AU  - Lin Tian
    AU  - Peili Wang
    AU  - Shenglan Wang
    Y1  - 2021/12/02
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ijde.20210604.15
    DO  - 10.11648/j.ijde.20210604.15
    T2  - International Journal of Diabetes and Endocrinology
    JF  - International Journal of Diabetes and Endocrinology
    JO  - International Journal of Diabetes and Endocrinology
    SP  - 150
    EP  - 159
    PB  - Science Publishing Group
    SN  - 2640-1371
    UR  - https://doi.org/10.11648/j.ijde.20210604.15
    AB  - Recent studies have demonstrated that pain is partially regulated by the gut microbiota. However, the association of gut microbiota with painful diabetic neuropathy, a common complication of diabetes, remains unclear. Herein, we investigated whether the gut microbiota is associated with mechanical allodynia during the early stage of hyperglycemia in 7-week-old db/db mice. The db/db mice were intraperitoneally injected with metformin for 2 weeks. Using the von Frey test and gut microbiota analyses, we investigated the association of gut microbial changes with mechanical allodynia in 7-week-old mice. In db/db mice, colonic microbial community profiles were altered, and both unweighted and weighted UniFrac distances were reduced. Colonic genus-level abundances of Alloprevotella and Prevotellaceae_UCG-001 were positively correlated with mechanical allodynia in db/db mice, while the abundance of Odoribacter was negatively correlated. Intraperitoneal injection of metformin for 2 weeks alleviated mechanical allodynia in db/db mice but did not achieve an anti-diabetic effect. Metformin altered colonic microbial communities and increased weighted UniFrac distance in db/db mice, although its analgesic effect was not associated with specific bacteria. Additionally, alteration of small intestinal microbial community profiles and reduction in weighted UniFrac distance were observed in db/db mice, which were not affected by metformin. These results provide potential evidence of the association of the gut microbiota with mechanical allodynia during early-stage of hyperglycemia.
    VL  - 6
    IS  - 4
    ER  - 

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Author Information
  • School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China

  • School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China

  • School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China

  • School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China

  • National Clinical Research Center for Chinese Medicine Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China

  • Department of Gerontology, Guang'anmen Hospital, China Academy of Chinese Medical Science, Beijing, China

  • National Clinical Research Center for Chinese Medicine Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China

  • School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China

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