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Antidiabetic, Antisickling and Antibacterial Activities of Anacardium occidentale L. (Anacardiaceae) and Zanthoxylum rubescens Planch. Ex Hook (Rutaceae) from DRC

Received: 22 February 2018     Accepted: 27 March 2018     Published: 4 May 2018
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Abstract

It was recently reported a rare association of two genetic diseases notably sickle cell anemia and diabetes in one patient in the Democratic Republic of the Congo. Both diseases constitute a serious public health problem and have a common denominator that is to make patients susceptible to bacterial infections. Given the difficult and limited management of these diseases, the use of medicinal plants is considered as an effective alternative. Leaves of Anacardium occidentale and Zanthoxylum rubescens collected in the surroundings of University of Kinshasa and Gbadolite in Kinshasa and Nord Ubangi provinces respectively and these plants were selected through a chemo-taxonomic approach while a phytochemical screening was performed using a qualitative approach. Bacterial strains used to assess the antibacterial activity were Staphylococcus aureus and Escherichia coli and mice were used for the antidiabetic activity. The phytochemical screening revealed the presence of tannins, anthocyanins, leucoanthocyanins, flavonoids, bound quinones as well saponins and alkaloids. The aqueous extracts of A. occidentale and Z. rubescens showed an antisickling activity. Only S. aureus was sensitive to A. occidentale where petroleum ether extract (MIC = 125 μg.mL-1) showed a good activity than other extracts and no activity was observed on E. coli. Meanwhile, Z. rubescens showed no antibacterial activity on both strains (MIC = > 500 μg.mL-1). The mean values of blood glucose after 120 minutes in untreated and treated mice were 99.5 ± 7.77mg.dL-1 (0.9% NaCl), 41.6 ± 10.07mg.dL-1 (Glibenclamide 10mg.Kg-1) and 64 ± 13.98mg.dL-1 (methanolic extract of A. occidentale 500mg.Kg-1). These findings show that A. occidentale plant possess an antihyperglycemic activity. To our knowledge, it is for the first time that the antisickling activity of A. occidentale and Z. rubescens is reported thus validating the chemotaxonomic approach used as a criterion of selection of these two plants. It is also for the first time that antidiabetic activity of A. occidentale is reported.

Published in International Journal of Diabetes and Endocrinology (Volume 3, Issue 1)
DOI 10.11648/j.ijde.20180301.12
Page(s) 7-14
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Co-Morbidity, Sickle Cell Anemia, Diabetes, Chemo-Taxonomic Approach

References
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Cite This Article
  • APA Style

    Gédéon Bongo, Koto-te-Nyiwa Ngbolua, Colette Ashande, Benjamin Gbolo, Claudine Tshiama, et al. (2018). Antidiabetic, Antisickling and Antibacterial Activities of Anacardium occidentale L. (Anacardiaceae) and Zanthoxylum rubescens Planch. Ex Hook (Rutaceae) from DRC. International Journal of Diabetes and Endocrinology, 3(1), 7-14. https://doi.org/10.11648/j.ijde.20180301.12

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    ACS Style

    Gédéon Bongo; Koto-te-Nyiwa Ngbolua; Colette Ashande; Benjamin Gbolo; Claudine Tshiama, et al. Antidiabetic, Antisickling and Antibacterial Activities of Anacardium occidentale L. (Anacardiaceae) and Zanthoxylum rubescens Planch. Ex Hook (Rutaceae) from DRC. Int. J. Diabetes Endocrinol. 2018, 3(1), 7-14. doi: 10.11648/j.ijde.20180301.12

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    AMA Style

    Gédéon Bongo, Koto-te-Nyiwa Ngbolua, Colette Ashande, Benjamin Gbolo, Claudine Tshiama, et al. Antidiabetic, Antisickling and Antibacterial Activities of Anacardium occidentale L. (Anacardiaceae) and Zanthoxylum rubescens Planch. Ex Hook (Rutaceae) from DRC. Int J Diabetes Endocrinol. 2018;3(1):7-14. doi: 10.11648/j.ijde.20180301.12

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  • @article{10.11648/j.ijde.20180301.12,
      author = {Gédéon Bongo and Koto-te-Nyiwa Ngbolua and Colette Ashande and Benjamin Gbolo and Claudine Tshiama and Dorothée Tshilanda and Damien Tshibangu and Nadège Ngombe and Théophile Mbemba and Pius Mpiana},
      title = {Antidiabetic, Antisickling and Antibacterial Activities of Anacardium occidentale L. (Anacardiaceae) and Zanthoxylum rubescens Planch. Ex Hook (Rutaceae) from DRC},
      journal = {International Journal of Diabetes and Endocrinology},
      volume = {3},
      number = {1},
      pages = {7-14},
      doi = {10.11648/j.ijde.20180301.12},
      url = {https://doi.org/10.11648/j.ijde.20180301.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijde.20180301.12},
      abstract = {It was recently reported a rare association of two genetic diseases notably sickle cell anemia and diabetes in one patient in the Democratic Republic of the Congo. Both diseases constitute a serious public health problem and have a common denominator that is to make patients susceptible to bacterial infections. Given the difficult and limited management of these diseases, the use of medicinal plants is considered as an effective alternative. Leaves of Anacardium occidentale and Zanthoxylum rubescens collected in the surroundings of University of Kinshasa and Gbadolite in Kinshasa and Nord Ubangi provinces respectively and these plants were selected through a chemo-taxonomic approach while a phytochemical screening was performed using a qualitative approach. Bacterial strains used to assess the antibacterial activity were Staphylococcus aureus and Escherichia coli and mice were used for the antidiabetic activity. The phytochemical screening revealed the presence of tannins, anthocyanins, leucoanthocyanins, flavonoids, bound quinones as well saponins and alkaloids. The aqueous extracts of A. occidentale and Z. rubescens showed an antisickling activity. Only S. aureus was sensitive to A. occidentale where petroleum ether extract (MIC = 125 μg.mL-1) showed a good activity than other extracts and no activity was observed on E. coli.  Meanwhile, Z. rubescens showed no antibacterial activity on both strains (MIC = > 500 μg.mL-1). The mean values of blood glucose after 120 minutes in untreated and treated mice were 99.5 ± 7.77mg.dL-1 (0.9% NaCl), 41.6 ± 10.07mg.dL-1 (Glibenclamide 10mg.Kg-1) and 64 ± 13.98mg.dL-1 (methanolic extract of A. occidentale 500mg.Kg-1). These findings show that A. occidentale plant possess an antihyperglycemic activity. To our knowledge, it is for the first time that the antisickling activity of A. occidentale and Z. rubescens is reported thus validating the chemotaxonomic approach used as a criterion of selection of these two plants. It is also for the first time that antidiabetic activity of A. occidentale is reported.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Antidiabetic, Antisickling and Antibacterial Activities of Anacardium occidentale L. (Anacardiaceae) and Zanthoxylum rubescens Planch. Ex Hook (Rutaceae) from DRC
    AU  - Gédéon Bongo
    AU  - Koto-te-Nyiwa Ngbolua
    AU  - Colette Ashande
    AU  - Benjamin Gbolo
    AU  - Claudine Tshiama
    AU  - Dorothée Tshilanda
    AU  - Damien Tshibangu
    AU  - Nadège Ngombe
    AU  - Théophile Mbemba
    AU  - Pius Mpiana
    Y1  - 2018/05/04
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ijde.20180301.12
    DO  - 10.11648/j.ijde.20180301.12
    T2  - International Journal of Diabetes and Endocrinology
    JF  - International Journal of Diabetes and Endocrinology
    JO  - International Journal of Diabetes and Endocrinology
    SP  - 7
    EP  - 14
    PB  - Science Publishing Group
    SN  - 2640-1371
    UR  - https://doi.org/10.11648/j.ijde.20180301.12
    AB  - It was recently reported a rare association of two genetic diseases notably sickle cell anemia and diabetes in one patient in the Democratic Republic of the Congo. Both diseases constitute a serious public health problem and have a common denominator that is to make patients susceptible to bacterial infections. Given the difficult and limited management of these diseases, the use of medicinal plants is considered as an effective alternative. Leaves of Anacardium occidentale and Zanthoxylum rubescens collected in the surroundings of University of Kinshasa and Gbadolite in Kinshasa and Nord Ubangi provinces respectively and these plants were selected through a chemo-taxonomic approach while a phytochemical screening was performed using a qualitative approach. Bacterial strains used to assess the antibacterial activity were Staphylococcus aureus and Escherichia coli and mice were used for the antidiabetic activity. The phytochemical screening revealed the presence of tannins, anthocyanins, leucoanthocyanins, flavonoids, bound quinones as well saponins and alkaloids. The aqueous extracts of A. occidentale and Z. rubescens showed an antisickling activity. Only S. aureus was sensitive to A. occidentale where petroleum ether extract (MIC = 125 μg.mL-1) showed a good activity than other extracts and no activity was observed on E. coli.  Meanwhile, Z. rubescens showed no antibacterial activity on both strains (MIC = > 500 μg.mL-1). The mean values of blood glucose after 120 minutes in untreated and treated mice were 99.5 ± 7.77mg.dL-1 (0.9% NaCl), 41.6 ± 10.07mg.dL-1 (Glibenclamide 10mg.Kg-1) and 64 ± 13.98mg.dL-1 (methanolic extract of A. occidentale 500mg.Kg-1). These findings show that A. occidentale plant possess an antihyperglycemic activity. To our knowledge, it is for the first time that the antisickling activity of A. occidentale and Z. rubescens is reported thus validating the chemotaxonomic approach used as a criterion of selection of these two plants. It is also for the first time that antidiabetic activity of A. occidentale is reported.
    VL  - 3
    IS  - 1
    ER  - 

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Author Information
  • Department of Biology, University de Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, University de Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Environmental Sciences, University of Gbadolite, Nord Ubangi, Democratic Republic of the Congo

  • Department of Biology, University de Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Teaching and Administration in Nursing Care, Higher Institute of Medical Techniques, Kinshasa, Democratic Republic of the Congo

  • Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, University de Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

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