International Journal of Applied Agricultural Sciences

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Experimental Study on the Effect of Low-Temperature Air Plasma Activated Water on Seed Germination and Seedling Growth of Maize

Received: Oct. 28, 2023    Accepted: Nov. 20, 2023    Published: Nov. 21, 2023
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Abstract

Plasma-activated water (PAW), which is produced by introducing non-thermal plasma into deionized water, contains variety of active substances such as reactive oxygen and reactive nitrogen species. These active substances in PAW exhibit sterilizing properties and facilitates intracellular chemical reactions, which holds the potential to enhance plant growth. This study aims to investigate the effects of PAW treatment on the process of maize seed germination and seedling growth. A gradient of different excitation voltages (10 kV, 14 kV, 18kV) and different treatment times (1min, 3min, 5min) was set to ionize the air to produce air-plasma, which was used to prepare the PAWs subsequently. The resulting PAWs were used to irrigate the maize seeds and seedlings every day. The effects on seed germination rate, germination potential, germination index, as well as on the chlorophyll content, peroxidase (POD) activity, and malondialdehyde (MDA) content in seedlings were examined. Treatment with PAW promoted seed germination and seedling growth, with the germination rate, germination potential, and germination index of the seeds increased by as much as 19.71%, 50.45% and 21.22% respectively. The chlorophyll and POD content in the seedlings also increased by 31.68% and 23.09% respectively. In addition, the MDA content decreased by 15.11% as compared with the control group (CK). The experimental results were subjected to significant variance analysis using the Duncan method.

DOI 10.11648/j.ijaas.20230906.12
Published in International Journal of Applied Agricultural Sciences ( Volume 9, Issue 6, November 2023 )
Page(s) 181-190
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Plasma-Activated Water, Maize, Seed Germination, Chlorophyll, Peroxidase, Malondialdehyde

References
[1] Guo D, Liu H, Zhou L et al. Plasma-activated water production and its application in agriculture. [J]. Journal of the Science of Food and Agriculture, 101 (12), 4891-4899 (2021).
[2] Bradu C, Kutasi K, Magureanu M et al. Reactive nitrogen species in plasma-activated water: generation, chemistry and application in agriculture. [J]. Journal of Physics D: Applied Physics 53 (22), 223001 (2020).
[3] Guo L, Xu R, Gou L et al. Mechanism of virus inactivation by cold atmospheric-pressure plasma and plasma-activated water. [J]. Applied and environmental microbiology 84 (17), e00726-18 (2018).
[4] Rathore V, Patel D, Butani S et al. Investigation of physicochemical properties of plasma activated water and its bactericidal efficacy. [J]. Plasma Chemistry and Plasma Processing 41, 871-902 (2021).
[5] Dumanović J, Nepovimova E, Natić M et al. The significance of reactive oxygen species and antioxidant defense system in plants: A concise overview. [J]. Frontiers in plant science. 11, 552969 (2021).
[6] Zambon Y, Contaldo N, Laurita R et al. Plasma activated water triggers plant defence responses. [J]. Scientific Reports. 10 (1), 19211 (2020).
[7] Guragain R P, Baniya H B, Shrestha B et al. Improvements in germination and growth of sprouts irrigated using plasma activated water (PAW). [J]. Water. 15 (4), 744 (2023).
[8] Škarpa P, Klofáč D, Krčma F et al. Effect of plasma activated water foliar application on selected growth parameters of maize (Zea mays L.). [J]. Water. 12 (12), 3545 (2020).
[9] Adhikari B, Adhikari M, Ghimire B et al. Cold atmospheric plasma-activated water irrigation induces defense hormone and gene expression in tomato seedlings. [J]. Scientific reports. 9 (1), 16080 (2019).
[10] Rathore V, Tiwari B S, Nema S K. Treatment of pea seeds with plasma activated water to enhance germination, plant growth, and plant composition. [J]. Plasma Chemistry and Plasma Processing, 1-21 (2022).
[11] Zhao Y, Chen R, Liu D et al. Effect of nonthermal plasma-activated water on quality and antioxidant activity of fresh-cut kiwifruit. [J]. IEEE Transactions on Plasma Science. 47 (11), 4811-4817 (2019).
[12] Cong K P, Li T T, Wu C E et al. Effects of plasma-activated water on overall quality of fresh goji berries during storage. [J]. Scientia Horticulturae. 293, 110650 (2022).
[13] Fan L, Liu X, Ma Y et al. Effects of plasma-activated water treatment on seed germination and growth of mung bean sprouts. [J]. Journal of Taibah University for Science. 14 (1), 823-830 (2020).
[14] Zhao Z, Wang X, Ma T. Properties of plasma-activated water with different activation time and its effects on the quality of button mushrooms (Agaricus bisporus). [J]. LWT. 147, 111633 (2021).
[15] Laurita R, Gozzi G, Tappi S et al. Effect of plasma activated water (PAW) on rocket leaves decontamination and nutritional value. [J]. Innovative Food Science & Emerging Technologies. 73, 102805 (2021).
[16] Ruan Z, Wang X, Liu Y et al. Corn [M] // Integrated Processing Technologies for Food and Agricultural By-Products. Academic Press, 59-72 (2019).
[17] Jin Y S, Cho C, Kim D et al. Mass production of plasma activated water by an atmospheric pressure plasma. [J]. Japanese Journal of Applied Physics. 59 (SH), SHHF05 (2020).
[18] Zhu Tong, Zhang Di, Tang Hongwei et al. Application of plasma activated water in seed pre-sowing treatment. [J]. Agricultural Engineering. 12 (10), 22-29 (2022).
[19] Ercoli L, Mariotti M, Masoni A et al. Relationship between nitrogen and chlorophyll content and spectral properties in maize leaves. [J]. European Journal of Agronomy. 2 (2), 113-117 (1993).
[20] Dumanović J, Nepovimova E, Natić M et al. The significance of reactive oxygen species and Bachmann L M, Miller W G. Spectrophotometry [M] // Contemporary Practice in Clinical Chemistry. Academic Press, 119-133 (2020).
[21] de Oliveira F K, Santos L O, Buffon J G. Mechanism of action, sources, and application of peroxidases. [J]. Food Research International. 143, 110266 (2021).
[22] Marnett L J. Lipid peroxidation—DNA damage by malondialdehyde. [J]. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 424 (1-2), 83-95 (1999).
[23] Valenzuela A. The biological significance of malondialdehyde determination in the assessment of tissue oxidative stress. [J]. Life sciences. 48 (4), 301-309 (1991).
[24] Chu Mengyuan, Yu Yanchong. Research progress on factors affecting plant leaf senescence, [J]. Life Sciences. 31 (2), 178-184 (2019).
[25] Ke Z, Bai Y, Yi Y et al. Why plasma-activated water treatment reduced the malonaldehyde content in muscle foods. [J]. Food Chemistry. 403, 134387 (2023).
[26] Tachibana K, Oh J S, Nakamura T. Oxidation processes of NO for production of reactive nitrogen species in plasma activated water. [J]. Journal of Physics D: Applied Physics. 53 (38), 385202 (2020).
[27] Grainge G, Nakabayashi K, Steinbrecher T et al. Molecular mechanisms of seed dormancy release by gas plasma-activated water technology. [J]. Journal of Experimental Botany. 73 (12), 4065-4078 (2022).
[28] Bafoil M, Jemmat A, Martinez Y et al. Effects of low temperature plasmas and plasma activated waters on Arabidopsis thaliana germination and growth. [J]. PloS one. 13 (4), e0195512 (2018).
[29] Porto C L, Ziuzina D, Los A et al. Plasma activated water and airborne ultrasound treatments for enhanced germination and growth of soybean. [J]. Innovative Food Science & Emerging Technologies. 49, 13-19 (2018).
[30] Rathore V, Nema S K. Optimization of process parameters to generate plasma activated water and study of physicochemical properties of plasma activated solutions at optimum condition. [J]. Journal of Applied Physics. 129 (8), 084901 (2021).
[31] Šimečková J, Krčma F, Klofáč D et al.: Influence of plasma-activated water on physical and physical–chemical soil properties. [J]. Water. 12 (9), 2357 (2020).
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    Wang, Z., Ren, T., Zhou, M., Chen, S., Fu, D., et al. (2023). Experimental Study on the Effect of Low-Temperature Air Plasma Activated Water on Seed Germination and Seedling Growth of Maize. International Journal of Applied Agricultural Sciences, 9(6), 181-190. https://doi.org/10.11648/j.ijaas.20230906.12

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    ACS Style

    Wang, Z.; Ren, T.; Zhou, M.; Chen, S.; Fu, D., et al. Experimental Study on the Effect of Low-Temperature Air Plasma Activated Water on Seed Germination and Seedling Growth of Maize. Int. J. Appl. Agric. Sci. 2023, 9(6), 181-190. doi: 10.11648/j.ijaas.20230906.12

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    AMA Style

    Wang Z, Ren T, Zhou M, Chen S, Fu D, et al. Experimental Study on the Effect of Low-Temperature Air Plasma Activated Water on Seed Germination and Seedling Growth of Maize. Int J Appl Agric Sci. 2023;9(6):181-190. doi: 10.11648/j.ijaas.20230906.12

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  • @article{10.11648/j.ijaas.20230906.12,
      author = {Zifeng Wang and Tianyi Ren and Minqi Zhou and Shuo Chen and Dexin Fu and Si Qin},
      title = {Experimental Study on the Effect of Low-Temperature Air Plasma Activated Water on Seed Germination and Seedling Growth of Maize},
      journal = {International Journal of Applied Agricultural Sciences},
      volume = {9},
      number = {6},
      pages = {181-190},
      doi = {10.11648/j.ijaas.20230906.12},
      url = {https://doi.org/10.11648/j.ijaas.20230906.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijaas.20230906.12},
      abstract = {Plasma-activated water (PAW), which is produced by introducing non-thermal plasma into deionized water, contains variety of active substances such as reactive oxygen and reactive nitrogen species. These active substances in PAW exhibit sterilizing properties and facilitates intracellular chemical reactions, which holds the potential to enhance plant growth. This study aims to investigate the effects of PAW treatment on the process of maize seed germination and seedling growth. A gradient of different excitation voltages (10 kV, 14 kV, 18kV) and different treatment times (1min, 3min, 5min) was set to ionize the air to produce air-plasma, which was used to prepare the PAWs subsequently. The resulting PAWs were used to irrigate the maize seeds and seedlings every day. The effects on seed germination rate, germination potential, germination index, as well as on the chlorophyll content, peroxidase (POD) activity, and malondialdehyde (MDA) content in seedlings were examined. Treatment with PAW promoted seed germination and seedling growth, with the germination rate, germination potential, and germination index of the seeds increased by as much as 19.71%, 50.45% and 21.22% respectively. The chlorophyll and POD content in the seedlings also increased by 31.68% and 23.09% respectively. In addition, the MDA content decreased by 15.11% as compared with the control group (CK). The experimental results were subjected to significant variance analysis using the Duncan method.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Experimental Study on the Effect of Low-Temperature Air Plasma Activated Water on Seed Germination and Seedling Growth of Maize
    AU  - Zifeng Wang
    AU  - Tianyi Ren
    AU  - Minqi Zhou
    AU  - Shuo Chen
    AU  - Dexin Fu
    AU  - Si Qin
    Y1  - 2023/11/21
    PY  - 2023
    N1  - https://doi.org/10.11648/j.ijaas.20230906.12
    DO  - 10.11648/j.ijaas.20230906.12
    T2  - International Journal of Applied Agricultural Sciences
    JF  - International Journal of Applied Agricultural Sciences
    JO  - International Journal of Applied Agricultural Sciences
    SP  - 181
    EP  - 190
    PB  - Science Publishing Group
    SN  - 2469-7885
    UR  - https://doi.org/10.11648/j.ijaas.20230906.12
    AB  - Plasma-activated water (PAW), which is produced by introducing non-thermal plasma into deionized water, contains variety of active substances such as reactive oxygen and reactive nitrogen species. These active substances in PAW exhibit sterilizing properties and facilitates intracellular chemical reactions, which holds the potential to enhance plant growth. This study aims to investigate the effects of PAW treatment on the process of maize seed germination and seedling growth. A gradient of different excitation voltages (10 kV, 14 kV, 18kV) and different treatment times (1min, 3min, 5min) was set to ionize the air to produce air-plasma, which was used to prepare the PAWs subsequently. The resulting PAWs were used to irrigate the maize seeds and seedlings every day. The effects on seed germination rate, germination potential, germination index, as well as on the chlorophyll content, peroxidase (POD) activity, and malondialdehyde (MDA) content in seedlings were examined. Treatment with PAW promoted seed germination and seedling growth, with the germination rate, germination potential, and germination index of the seeds increased by as much as 19.71%, 50.45% and 21.22% respectively. The chlorophyll and POD content in the seedlings also increased by 31.68% and 23.09% respectively. In addition, the MDA content decreased by 15.11% as compared with the control group (CK). The experimental results were subjected to significant variance analysis using the Duncan method.
    VL  - 9
    IS  - 6
    ER  - 

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Author Information
  • School of Electrical Engineering, Guangxi University, Nanning, China

  • School of Electrical Engineering, Guangxi University, Nanning, China

  • School of Electrical Engineering, Guangxi University, Nanning, China

  • School of Electrical Engineering, Guangxi University, Nanning, China

  • School of Electrical Engineering, Guangxi University, Nanning, China

  • School of Electrical Engineering, Guangxi University, Nanning, China

  • Section