This study was designed to screen microorganisms from decomposing palm kernel shaft for cellulase palm oil processing sites in Akoko area of Ondo State, Nigeria. Isolation of microorganisms was carried out by serial dilution and pour plate methods andidentified using standard biochemical methods. The isolates were screened for cellulase production using standard assay methods. The microorganisms were grown in a mineral salt basal medium for maximum yield of cellulase production. The microorganisms isolated from the sample include Bacillus subtilis, Micrococcus varians, M. leteus, Cellulomonas blazotes, C. flavigina, Sarcina ventriculi, B. cereus, C. fimi, Aspergillus niger, Rhizopus stolonifer, Saccharomyces cerevisiae and Trichoderma viridae. The screened microorganisms exhibited varied cellulase activities. The diameters of zones of clearance of the isolates ranged from 1.45 to 1.83for bacteria and 0.00 to 2.06for fungi. The cellulase activity exhibited by bacteria ranged from 0.238 µmol/ml to 0.590 µmol/ml while fungal cellulase activity ranged from 0.452 µmol/ml to 0.775 µmol/ml. The high cellulase activity exhibited by fungi isolated from decomposing palm kernel shaft suggested that their predominance as a potential source of cellulase could be more promising in various industrial processes for the degradation of cellulose containing organic substances.
| Published in | Frontiers in Environmental Microbiology (Volume 5, Issue 5) |
| DOI | 10.11648/j.fem.20190505.11 |
| Page(s) | 100-105 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
Cellulose, Wastes, Palm Kernel Shaft, Cellulose, Microorganisms
| [1] | Akinyele, B. J. (2010). Biotransformation of cassava wastes by fungal fermentation. Applied Tropical Agriculture. 15 (2): 90-96. |
| [2] | Olaniyi, O. O. and Arotupin, D. J. (2013). Isolation and screening of mannase producing bacteria from agricultural wastes. British Microbiology Research Journal. 3 (4): 654-663. |
| [3] | Adeniyi, O. R., Ogunsola, G. O. and Oluwusi, D. (2014). Methods of palm oil processing in Ogun state, Nigeria: A resource use efficiency assessment. American International Journal of Contemporary Research. 4 (8): 173-179. |
| [4] | Rupani, P. F., Singh, R. P., Ibrahim, M. H. and Eso, N. (2010). Review of current palm oil mill effluent (pome) treatment methods: Vermicomposting as a sustainable practice. World Applied Sciences Journal. 10 (1): 1190-1201. |
| [5] | Chanzy, H. (1990). Aspects of cellulose structure. In Kennedy J. F. and Philips Gro and Williams, P. A. (ed). Cellulose sources and Exploitation; ellisHorwood, New York. pp 3-12. |
| [6] | Samuelsson, G., (1994). Drugs of natural origin. A textbook of pharmacology, Swedish pharmaceutical press. Second Edition. pp 130-138. |
| [7] | Norita, S. M., Rosfarizan, M. and Ariff, A. B. (2010). Evaluation of the activities of concentrated crude mannan-degrading enzymes produced by Aspergillus niger. Malaysian Journal of Microbiology. 6: 171-180. |
| [8] | Ekundayo, T. C. and Arotupin, D. J. (2015). Isolation and identification of cellulolytic fungi from agrowastes and sawmill soils. British Biotechnology Journal. 7 (3): 147-159. |
| [9] | Gautam, S. P., Budela, P. S., Pandey, A. K., Jamaluddin, A. M. K. and Sarsaiya, S. (2010). Optimization of the medium for the production of cellulase by the Trichoderma viridae using submerged fermentation. International Journal of Environmental Science. 4 (1): 656-665. |
| [10] | Holt, J. G., Kneg, N. R., Sneath, P. H., Stanley, J. J. and Williams, S. T. (1994). Bergey’s Manual of Determinative Bacteriology. Wilkins Publishers, Baltimore. pp 136-141. |
| [11] | Alexopoulus, C. J., Mims, C. M. and Blackwell, M. (1996). Introductory Mycology. 4th Edition, John Wiley and Sons, New York. 868 p. |
| [12] | Immanuel, G., Dhanusa, R., Prema, P. and Palavesam, A. (2006). Effect of different growth parameters on endoglucanase enzyme activity by bacteria isolated from coir retting effluents of estuarine environment. International Journal of Environment Science and Technology. 3 (1): 25-34. |
| [13] | Saraswati, B., Ravi-Kumar, M., Mukesh-Kumar, D. J., Balashanmugam, P., Bala-Kumaran, M. D. and Kalaichelvan, P. T. (2012). Cellulase production by Bacillus subtilis isolated from cow dung. Archives of Applied Science Research. 4 (1): 269-279. |
| [14] | Amaeze, N. J., Okoliegbe, I. N. and Francis, M. E. (2015). Cellulase production by Aspergillus niger and Saccharomyces cerevisiae using fruit wastes as substrates. International Journal of Applied Microbiology and Biotechnology Research. 3: 36-44. |
| [15] | Lowry, O. H., Resebrough, N. J., Farr, A. L. and Randal, R. J. (1951). Protein measurement with folin phenol reagent Journal of Biology and Chemistry. 193: 265-275. |
| [16] | Akinyele, B. J., Olaniyi, O. O. and Arotupin, D. J. (2011). Bioconversion of selected of selected agricultural wastes and associated enzymes by Volvariella volvacea: An edible mushroom. Research Journal of Microbiology. 12: 567-573. |
| [17] | Sethi, S., Datta, A. L., Gupta, B., Gupta, S. (2013). Optimization of cellulase production from bacteria isolated from soil. Biotechnology. 985685, 1-7. |
| [18] | Eno, E. O., Antai, S. P. and Tiku, D. R. (2017). Microbiological and physicochemical impact of palm oil mill effluent on the surrounding soil at selected factory location within Calabar and Uyo. Imperial Journal of Interdisciplinary Research. 3 (10): 760-773. |
| [19] | Al-Qahtani, A. N., Geweely, N. S. and AL-Fasi, F. A. (2013). Radiation mutagenesis and purification of xylanase produced by soil fungi. International Research Journal of Agricultural Science and Soil Science. 3 (5): 156-168. |
| [20] | Blibech, M., EllouzGhorbel, M. R. and Fakhfakh, I. (2010). Purification and characterization of a low molecular weight of β- mannanase from Penicillium occitanis Pol6. Applied Biochemistry and Biotechnology. 160: 1227-1240. |
| [21] | Saowapar, K., Yupa, P., Taweesak, T. and Somboon, T. (2014). Screening and identification of cellulase producing bacteria isolated from oil palm meal. Journal of Applied Pharmaceutical Science. 4 (04): 090-096. |
| [22] | Ankita, M., Suresh, S. and Sarika, S. (2014). Purification and characterization of thermostable amylase from Psychrophile. Octa Journal of Environmental Research. 2 (1): 38-47. |
| [23] | Odeyemi, A. T., Aderiye, B. I., Adeyeye, E. I., Donbraye, E. and Faleye, T. (2014). Lipolytic activity and molecular identification of Pseudomonas aeruginosa and Lysinibacillus sphaericus isolated from domestic oil rich wastewater. British Microbiology Research Journal. 4 (4): 392-404. |
| [24] | Aderiye, B. I., Adebayo, A. A. and Mustapha, B. (2017). Bioaccumulation of heavy metals and optimization of lipase production by Lysinibacilluss phaericus strain ODE16_EKITI isolated from domestic oil-rich wastewater. International Journal of Current Microbiology and Applied Sciences. 6 (8): 3790-3802. |
| [25] | Abeer, A. K., Foukia, E. M. and Eman, A. K. (2016). Exopolygalacturonase production from Jojoba mill solid waste by Aspergillus oryzae FK-923 under solid state fermentation. Journal of Pharmaceutical, Biological and Chemical Sciences. 7 (1): 447-454. |
| [26] | Khokhar, I., Mukhtar, I. and Mushtaq, S. (2011). Isolation and screening of amylolytic filamentous fungi. Journal of Applied Science and Environmental Management. 8 (1): 203-206. |
| [27] | Amir, I., Zahid, A., Yusuf, Z., Iqbal, H., Aish, M., Muhammad, I. and Sajid, M. (2011). Optimization of cellulase enzyme production from corn cobs using Alternaria alternate by solid state fermentation. Journal of Cell and Molecular Biology. 9 (2): 51-56. |
| [28] | Olaniyi, O. O., Igbe, F. O. and Ekundayo, T. C. (2013). Optimization studies on mannanase production by Trichosporonoides oedocephalis in submerged state fermentation. E3 Journal of Biotechnology and Pharmaceutical. 4 (7): 110-116. |
| [29] | Damisa, D., Kuta, F. A. and Adabara, N. U. (2013). Mutagenic treatment of Aspergillus niger with hydroxylamine for improved cellulase synthesis from cellulosic wastes. Journal of Biotechnological Sciences. 1 (2): 65-72. |
| [30] | Akinyele, B. J., Ekundayo, T. C. Olaniyi, O. O. (2013). Parameters optimization of cellulose zymosynthesis by Aspergillus flavus NSPR017 grown on pretreated orange peels. Nature and Science. 11 (10): 80-87. |
| [31] | Omemu, A. M., Bamigbade, G., Obadina, A. O. and Obuotor, T. M. (2015). Isolation and screening of amylase from moulds associated with the spoilage of some fermented cereal foods. British Microbiology Research Journal. 5 (4): 359-367. |
| [32] | Muhammad, I., Asma, S., Quratulain, S. and Muhammad, N. (2012). Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity. Turkey Journal of Biochemistry. 37 (3): 287 293. |
APA Style
Adegbehingbe Kehinde Tope, Bello Marcus Oluyemi, Fakoya Soji, Adeleke Bartholomew Saanu, Jemilaiye Taiye Anangwureyi, et al. (2020). Isolation and Screening of Microbial Isolates from Decomposing Palm Kernel Shaft for Cellulase Production. Frontiers in Environmental Microbiology, 5(5), 100-105. https://doi.org/10.11648/j.fem.20190505.11
ACS Style
Adegbehingbe Kehinde Tope; Bello Marcus Oluyemi; Fakoya Soji; Adeleke Bartholomew Saanu; Jemilaiye Taiye Anangwureyi, et al. Isolation and Screening of Microbial Isolates from Decomposing Palm Kernel Shaft for Cellulase Production. Front. Environ. Microbiol. 2020, 5(5), 100-105. doi: 10.11648/j.fem.20190505.11
AMA Style
Adegbehingbe Kehinde Tope, Bello Marcus Oluyemi, Fakoya Soji, Adeleke Bartholomew Saanu, Jemilaiye Taiye Anangwureyi, et al. Isolation and Screening of Microbial Isolates from Decomposing Palm Kernel Shaft for Cellulase Production. Front Environ Microbiol. 2020;5(5):100-105. doi: 10.11648/j.fem.20190505.11
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Download@article{10.11648/j.fem.20190505.11,
author = {Adegbehingbe Kehinde Tope and Bello Marcus Oluyemi and Fakoya Soji and Adeleke Bartholomew Saanu and Jemilaiye Taiye Anangwureyi and Orege Samuel Temidire},
title = {Isolation and Screening of Microbial Isolates from Decomposing Palm Kernel Shaft for Cellulase Production},
journal = {Frontiers in Environmental Microbiology},
volume = {5},
number = {5},
pages = {100-105},
doi = {10.11648/j.fem.20190505.11},
url = {https://doi.org/10.11648/j.fem.20190505.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20190505.11},
abstract = {This study was designed to screen microorganisms from decomposing palm kernel shaft for cellulase palm oil processing sites in Akoko area of Ondo State, Nigeria. Isolation of microorganisms was carried out by serial dilution and pour plate methods andidentified using standard biochemical methods. The isolates were screened for cellulase production using standard assay methods. The microorganisms were grown in a mineral salt basal medium for maximum yield of cellulase production. The microorganisms isolated from the sample include Bacillus subtilis, Micrococcus varians, M. leteus, Cellulomonas blazotes, C. flavigina, Sarcina ventriculi, B. cereus, C. fimi, Aspergillus niger, Rhizopus stolonifer, Saccharomyces cerevisiae and Trichoderma viridae. The screened microorganisms exhibited varied cellulase activities. The diameters of zones of clearance of the isolates ranged from 1.45 to 1.83for bacteria and 0.00 to 2.06for fungi. The cellulase activity exhibited by bacteria ranged from 0.238 µmol/ml to 0.590 µmol/ml while fungal cellulase activity ranged from 0.452 µmol/ml to 0.775 µmol/ml. The high cellulase activity exhibited by fungi isolated from decomposing palm kernel shaft suggested that their predominance as a potential source of cellulase could be more promising in various industrial processes for the degradation of cellulose containing organic substances.},
year = {2020}
}
TY - JOUR T1 - Isolation and Screening of Microbial Isolates from Decomposing Palm Kernel Shaft for Cellulase Production AU - Adegbehingbe Kehinde Tope AU - Bello Marcus Oluyemi AU - Fakoya Soji AU - Adeleke Bartholomew Saanu AU - Jemilaiye Taiye Anangwureyi AU - Orege Samuel Temidire Y1 - 2020/01/06 PY - 2020 N1 - https://doi.org/10.11648/j.fem.20190505.11 DO - 10.11648/j.fem.20190505.11 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 100 EP - 105 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20190505.11 AB - This study was designed to screen microorganisms from decomposing palm kernel shaft for cellulase palm oil processing sites in Akoko area of Ondo State, Nigeria. Isolation of microorganisms was carried out by serial dilution and pour plate methods andidentified using standard biochemical methods. The isolates were screened for cellulase production using standard assay methods. The microorganisms were grown in a mineral salt basal medium for maximum yield of cellulase production. The microorganisms isolated from the sample include Bacillus subtilis, Micrococcus varians, M. leteus, Cellulomonas blazotes, C. flavigina, Sarcina ventriculi, B. cereus, C. fimi, Aspergillus niger, Rhizopus stolonifer, Saccharomyces cerevisiae and Trichoderma viridae. The screened microorganisms exhibited varied cellulase activities. The diameters of zones of clearance of the isolates ranged from 1.45 to 1.83for bacteria and 0.00 to 2.06for fungi. The cellulase activity exhibited by bacteria ranged from 0.238 µmol/ml to 0.590 µmol/ml while fungal cellulase activity ranged from 0.452 µmol/ml to 0.775 µmol/ml. The high cellulase activity exhibited by fungi isolated from decomposing palm kernel shaft suggested that their predominance as a potential source of cellulase could be more promising in various industrial processes for the degradation of cellulose containing organic substances. VL - 5 IS - 5 ER -
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