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Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production

Received: 9 April 2019     Accepted: 27 May 2019     Published: 5 August 2019
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Abstract

Free living nitrogen fixing bacteria are those that lives freely on rhizosphere of a young growing plant or those living in a close association with root of plants (Rhizobacteria) but not symbiotically. In most tropical soil, Nitrogen and other essential nutrients element are the most limiting nutrients which deficiencies could lead to slow growth and reduced crop production. Free living nitrogen fixing bacteria has the potential of secreting nitrogenase enzymes and produces organic acids such as glycolic, acetic, malic, succunic acids which fix atmospheric nitrogen directly into the soil for plants growth and development. To this end, the study on isolation, identification and screening of Azotobacter chroococcum from soil of Keffi, Nasarawa State, Nigeria was carried out from May to September 2018. Soil samples were collected from eight different locations and Azotobacter strains were isolated and identified using standard microbiological methods. The 16SrRNA gene sequence analysis of the strain showed maximum similarity of 96% with Azotobacter chroococcum of the reference type strain deposited in RDP Gen Bank database. Azotobacter strains isolated from four different locations showed coloured zone ranging between 16-10mm. Similarly, Percentage amount of nitrogen released by each Azotobacter strain in the culture broth ranging between 1.19% - 5.11% in an increasing order.

Published in Frontiers in Environmental Microbiology (Volume 5, Issue 3)
DOI 10.11648/j.fem.20190503.11
Page(s) 70-76
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Rhizobacteria, Azotobacter chroococcum, 16SrRNA Sequencing, Nitrogen-free Agar, Nitrogen-free Broth, Bio-fertilizer

References
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Cite This Article
  • APA Style

    Ishaya Isa Obele, Makut Makwin Danladi, Ombugadu Akwashiki, Gabriel Owuna, Odonye Enoch Peter, et al. (2019). Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production. Frontiers in Environmental Microbiology, 5(3), 70-76. https://doi.org/10.11648/j.fem.20190503.11

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    ACS Style

    Ishaya Isa Obele; Makut Makwin Danladi; Ombugadu Akwashiki; Gabriel Owuna; Odonye Enoch Peter, et al. Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production. Front. Environ. Microbiol. 2019, 5(3), 70-76. doi: 10.11648/j.fem.20190503.11

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    AMA Style

    Ishaya Isa Obele, Makut Makwin Danladi, Ombugadu Akwashiki, Gabriel Owuna, Odonye Enoch Peter, et al. Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production. Front Environ Microbiol. 2019;5(3):70-76. doi: 10.11648/j.fem.20190503.11

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  • @article{10.11648/j.fem.20190503.11,
      author = {Ishaya Isa Obele and Makut Makwin Danladi and Ombugadu Akwashiki and Gabriel Owuna and Odonye Enoch Peter and Smart Obiekezie and Tsaku Paul and Ekeleme Ikke Kenneth and Akirinola-Akinyemi Olokunle},
      title = {Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production},
      journal = {Frontiers in Environmental Microbiology},
      volume = {5},
      number = {3},
      pages = {70-76},
      doi = {10.11648/j.fem.20190503.11},
      url = {https://doi.org/10.11648/j.fem.20190503.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20190503.11},
      abstract = {Free living nitrogen fixing bacteria are those that lives freely on rhizosphere of a young growing plant or those living in a close association with root of plants (Rhizobacteria) but not symbiotically. In most tropical soil, Nitrogen and other essential nutrients element are the most limiting nutrients which deficiencies could lead to slow growth and reduced crop production. Free living nitrogen fixing bacteria has the potential of secreting nitrogenase enzymes and produces organic acids such as glycolic, acetic, malic, succunic acids which fix atmospheric nitrogen directly into the soil for plants growth and development. To this end, the study on isolation, identification and screening of Azotobacter chroococcum from soil of Keffi, Nasarawa State, Nigeria was carried out from May to September 2018. Soil samples were collected from eight different locations and Azotobacter strains were isolated and identified using standard microbiological methods. The 16SrRNA gene sequence analysis of the strain showed maximum similarity of 96% with Azotobacter chroococcum of the reference type strain deposited in RDP Gen Bank database. Azotobacter strains isolated from four different locations showed coloured zone ranging between 16-10mm. Similarly, Percentage amount of nitrogen released by each Azotobacter strain in the culture broth ranging between 1.19% - 5.11% in an increasing order.},
     year = {2019}
    }
    

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    AU  - Ishaya Isa Obele
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    AU  - Gabriel Owuna
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    AU  - Akirinola-Akinyemi Olokunle
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    T2  - Frontiers in Environmental Microbiology
    JF  - Frontiers in Environmental Microbiology
    JO  - Frontiers in Environmental Microbiology
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    AB  - Free living nitrogen fixing bacteria are those that lives freely on rhizosphere of a young growing plant or those living in a close association with root of plants (Rhizobacteria) but not symbiotically. In most tropical soil, Nitrogen and other essential nutrients element are the most limiting nutrients which deficiencies could lead to slow growth and reduced crop production. Free living nitrogen fixing bacteria has the potential of secreting nitrogenase enzymes and produces organic acids such as glycolic, acetic, malic, succunic acids which fix atmospheric nitrogen directly into the soil for plants growth and development. To this end, the study on isolation, identification and screening of Azotobacter chroococcum from soil of Keffi, Nasarawa State, Nigeria was carried out from May to September 2018. Soil samples were collected from eight different locations and Azotobacter strains were isolated and identified using standard microbiological methods. The 16SrRNA gene sequence analysis of the strain showed maximum similarity of 96% with Azotobacter chroococcum of the reference type strain deposited in RDP Gen Bank database. Azotobacter strains isolated from four different locations showed coloured zone ranging between 16-10mm. Similarly, Percentage amount of nitrogen released by each Azotobacter strain in the culture broth ranging between 1.19% - 5.11% in an increasing order.
    VL  - 5
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Author Information
  • Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria

  • Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria

  • Department of Zoology, Faculty of Science, Federal University Lafia, Nasarawa, Nigeria

  • Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria

  • Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria

  • Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria

  • Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria

  • Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria

  • Federal Institutes of Industrial Research, Oshodi Lagos, Nigeria

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