Isolation, Identification and Screening for Nitrogen Fixing Activities by <i>Azotobacter chroococcum</i> Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production

Ishaya Isa Obele; Makut Makwin Danladi; Ombugadu Akwashiki; Gabriel Owuna; Odonye Enoch Peter; Smart Obiekezie; Tsaku Paul; Ekeleme Ikke Kenneth; Akirinola-Akinyemi Olokunle

doi:doi:10.11648/j.fem.20190503.11

Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production

Ishaya Isa Obele, Makut Makwin Danladi, Ombugadu Akwashiki, Gabriel Owuna, Odonye Enoch Peter, Smart Obiekezie, Tsaku Paul, Ekeleme Ikke Kenneth, Akirinola-Akinyemi Olokunle

Published in Frontiers in Environmental Microbiology (Volume 5, Issue 3)

Received: 9 April 2019 Accepted: 27 May 2019 Published: 5 August 2019

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Abstract

Free living nitrogen fixing bacteria are those that lives freely on rhizosphere of a young growing plant or those living in a close association with root of plants (Rhizobacteria) but not symbiotically. In most tropical soil, Nitrogen and other essential nutrients element are the most limiting nutrients which deficiencies could lead to slow growth and reduced crop production. Free living nitrogen fixing bacteria has the potential of secreting nitrogenase enzymes and produces organic acids such as glycolic, acetic, malic, succunic acids which fix atmospheric nitrogen directly into the soil for plants growth and development. To this end, the study on isolation, identification and screening of Azotobacter chroococcum from soil of Keffi, Nasarawa State, Nigeria was carried out from May to September 2018. Soil samples were collected from eight different locations and Azotobacter strains were isolated and identified using standard microbiological methods. The 16SrRNA gene sequence analysis of the strain showed maximum similarity of 96% with Azotobacter chroococcum of the reference type strain deposited in RDP Gen Bank database. Azotobacter strains isolated from four different locations showed coloured zone ranging between 16-10mm. Similarly, Percentage amount of nitrogen released by each Azotobacter strain in the culture broth ranging between 1.19% - 5.11% in an increasing order.

Published in	Frontiers in Environmental Microbiology (Volume 5, Issue 3)
DOI	10.11648/j.fem.20190503.11
Page(s)	70-76
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Rhizobacteria, Azotobacter chroococcum, 16SrRNA Sequencing, Nitrogen-free Agar, Nitrogen-free Broth, Bio-fertilizer

References

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APA Style

Ishaya Isa Obele, Makut Makwin Danladi, Ombugadu Akwashiki, Gabriel Owuna, Odonye Enoch Peter, et al. (2019). Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production. Frontiers in Environmental Microbiology, 5(3), 70-76. https://doi.org/10.11648/j.fem.20190503.11

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ACS Style

Ishaya Isa Obele; Makut Makwin Danladi; Ombugadu Akwashiki; Gabriel Owuna; Odonye Enoch Peter, et al. Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production. Front. Environ. Microbiol. 2019, 5(3), 70-76. doi: 10.11648/j.fem.20190503.11

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AMA Style

Ishaya Isa Obele, Makut Makwin Danladi, Ombugadu Akwashiki, Gabriel Owuna, Odonye Enoch Peter, et al. Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production. Front Environ Microbiol. 2019;5(3):70-76. doi: 10.11648/j.fem.20190503.11

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@article{10.11648/j.fem.20190503.11,
  author = {Ishaya Isa Obele and Makut Makwin Danladi and Ombugadu Akwashiki and Gabriel Owuna and Odonye Enoch Peter and Smart Obiekezie and Tsaku Paul and Ekeleme Ikke Kenneth and Akirinola-Akinyemi Olokunle},
  title = {Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production},
  journal = {Frontiers in Environmental Microbiology},
  volume = {5},
  number = {3},
  pages = {70-76},
  doi = {10.11648/j.fem.20190503.11},
  url = {https://doi.org/10.11648/j.fem.20190503.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20190503.11},
  abstract = {Free living nitrogen fixing bacteria are those that lives freely on rhizosphere of a young growing plant or those living in a close association with root of plants (Rhizobacteria) but not symbiotically. In most tropical soil, Nitrogen and other essential nutrients element are the most limiting nutrients which deficiencies could lead to slow growth and reduced crop production. Free living nitrogen fixing bacteria has the potential of secreting nitrogenase enzymes and produces organic acids such as glycolic, acetic, malic, succunic acids which fix atmospheric nitrogen directly into the soil for plants growth and development. To this end, the study on isolation, identification and screening of Azotobacter chroococcum from soil of Keffi, Nasarawa State, Nigeria was carried out from May to September 2018. Soil samples were collected from eight different locations and Azotobacter strains were isolated and identified using standard microbiological methods. The 16SrRNA gene sequence analysis of the strain showed maximum similarity of 96% with Azotobacter chroococcum of the reference type strain deposited in RDP Gen Bank database. Azotobacter strains isolated from four different locations showed coloured zone ranging between 16-10mm. Similarly, Percentage amount of nitrogen released by each Azotobacter strain in the culture broth ranging between 1.19% - 5.11% in an increasing order.},
 year = {2019}
}

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TY  - JOUR
T1  - Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production
AU  - Ishaya Isa Obele
AU  - Makut Makwin Danladi
AU  - Ombugadu Akwashiki
AU  - Gabriel Owuna
AU  - Odonye Enoch Peter
AU  - Smart Obiekezie
AU  - Tsaku Paul
AU  - Ekeleme Ikke Kenneth
AU  - Akirinola-Akinyemi Olokunle
Y1  - 2019/08/05
PY  - 2019
N1  - https://doi.org/10.11648/j.fem.20190503.11
DO  - 10.11648/j.fem.20190503.11
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 70
EP  - 76
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20190503.11
AB  - Free living nitrogen fixing bacteria are those that lives freely on rhizosphere of a young growing plant or those living in a close association with root of plants (Rhizobacteria) but not symbiotically. In most tropical soil, Nitrogen and other essential nutrients element are the most limiting nutrients which deficiencies could lead to slow growth and reduced crop production. Free living nitrogen fixing bacteria has the potential of secreting nitrogenase enzymes and produces organic acids such as glycolic, acetic, malic, succunic acids which fix atmospheric nitrogen directly into the soil for plants growth and development. To this end, the study on isolation, identification and screening of Azotobacter chroococcum from soil of Keffi, Nasarawa State, Nigeria was carried out from May to September 2018. Soil samples were collected from eight different locations and Azotobacter strains were isolated and identified using standard microbiological methods. The 16SrRNA gene sequence analysis of the strain showed maximum similarity of 96% with Azotobacter chroococcum of the reference type strain deposited in RDP Gen Bank database. Azotobacter strains isolated from four different locations showed coloured zone ranging between 16-10mm. Similarly, Percentage amount of nitrogen released by each Azotobacter strain in the culture broth ranging between 1.19% - 5.11% in an increasing order.
VL  - 5
IS  - 3
ER  -

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Author Information

Ishaya Isa Obele

Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria
Makut Makwin Danladi

Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria
Ombugadu Akwashiki

Department of Zoology, Faculty of Science, Federal University Lafia, Nasarawa, Nigeria
Gabriel Owuna

Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria
Odonye Enoch Peter

Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria
Smart Obiekezie

Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria
Tsaku Paul

Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria
Ekeleme Ikke Kenneth

Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University, Nasarawa, Nigeria
Akirinola-Akinyemi Olokunle

Federal Institutes of Industrial Research, Oshodi Lagos, Nigeria

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Plain Text BibTeX RIS

APA Style

Ishaya Isa Obele, Makut Makwin Danladi, Ombugadu Akwashiki, Gabriel Owuna, Odonye Enoch Peter, et al. (2019). Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production. Frontiers in Environmental Microbiology, 5(3), 70-76. https://doi.org/10.11648/j.fem.20190503.11

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ACS Style

Ishaya Isa Obele; Makut Makwin Danladi; Ombugadu Akwashiki; Gabriel Owuna; Odonye Enoch Peter, et al. Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production. Front. Environ. Microbiol. 2019, 5(3), 70-76. doi: 10.11648/j.fem.20190503.11

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AMA Style

Ishaya Isa Obele, Makut Makwin Danladi, Ombugadu Akwashiki, Gabriel Owuna, Odonye Enoch Peter, et al. Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production. Front Environ Microbiol. 2019;5(3):70-76. doi: 10.11648/j.fem.20190503.11

Copy | Download

@article{10.11648/j.fem.20190503.11,
  author = {Ishaya Isa Obele and Makut Makwin Danladi and Ombugadu Akwashiki and Gabriel Owuna and Odonye Enoch Peter and Smart Obiekezie and Tsaku Paul and Ekeleme Ikke Kenneth and Akirinola-Akinyemi Olokunle},
  title = {Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production},
  journal = {Frontiers in Environmental Microbiology},
  volume = {5},
  number = {3},
  pages = {70-76},
  doi = {10.11648/j.fem.20190503.11},
  url = {https://doi.org/10.11648/j.fem.20190503.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20190503.11},
  abstract = {Free living nitrogen fixing bacteria are those that lives freely on rhizosphere of a young growing plant or those living in a close association with root of plants (Rhizobacteria) but not symbiotically. In most tropical soil, Nitrogen and other essential nutrients element are the most limiting nutrients which deficiencies could lead to slow growth and reduced crop production. Free living nitrogen fixing bacteria has the potential of secreting nitrogenase enzymes and produces organic acids such as glycolic, acetic, malic, succunic acids which fix atmospheric nitrogen directly into the soil for plants growth and development. To this end, the study on isolation, identification and screening of Azotobacter chroococcum from soil of Keffi, Nasarawa State, Nigeria was carried out from May to September 2018. Soil samples were collected from eight different locations and Azotobacter strains were isolated and identified using standard microbiological methods. The 16SrRNA gene sequence analysis of the strain showed maximum similarity of 96% with Azotobacter chroococcum of the reference type strain deposited in RDP Gen Bank database. Azotobacter strains isolated from four different locations showed coloured zone ranging between 16-10mm. Similarly, Percentage amount of nitrogen released by each Azotobacter strain in the culture broth ranging between 1.19% - 5.11% in an increasing order.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Isolation, Identification and Screening for Nitrogen Fixing Activities by Azotobacter chroococcum Isolated from Soil of Keffi, Nigeria as Agent for Bio-fertilizer Production
AU  - Ishaya Isa Obele
AU  - Makut Makwin Danladi
AU  - Ombugadu Akwashiki
AU  - Gabriel Owuna
AU  - Odonye Enoch Peter
AU  - Smart Obiekezie
AU  - Tsaku Paul
AU  - Ekeleme Ikke Kenneth
AU  - Akirinola-Akinyemi Olokunle
Y1  - 2019/08/05
PY  - 2019
N1  - https://doi.org/10.11648/j.fem.20190503.11
DO  - 10.11648/j.fem.20190503.11
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 70
EP  - 76
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20190503.11
AB  - Free living nitrogen fixing bacteria are those that lives freely on rhizosphere of a young growing plant or those living in a close association with root of plants (Rhizobacteria) but not symbiotically. In most tropical soil, Nitrogen and other essential nutrients element are the most limiting nutrients which deficiencies could lead to slow growth and reduced crop production. Free living nitrogen fixing bacteria has the potential of secreting nitrogenase enzymes and produces organic acids such as glycolic, acetic, malic, succunic acids which fix atmospheric nitrogen directly into the soil for plants growth and development. To this end, the study on isolation, identification and screening of Azotobacter chroococcum from soil of Keffi, Nasarawa State, Nigeria was carried out from May to September 2018. Soil samples were collected from eight different locations and Azotobacter strains were isolated and identified using standard microbiological methods. The 16SrRNA gene sequence analysis of the strain showed maximum similarity of 96% with Azotobacter chroococcum of the reference type strain deposited in RDP Gen Bank database. Azotobacter strains isolated from four different locations showed coloured zone ranging between 16-10mm. Similarly, Percentage amount of nitrogen released by each Azotobacter strain in the culture broth ranging between 1.19% - 5.11% in an increasing order.
VL  - 5
IS  - 3
ER  -

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