Diversity and Metabolic Potential of the Dominant Culturable N<sub>2</sub>-Fixing and P-Solubilising Bacteria from Tea (<i>Camellia sinensis</i> L.) Rhizosphere

Atefeh Varmazyari; Ramazan Çakmakçı

doi:doi:10.11648/j.fem.20180402.12

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Diversity and Metabolic Potential of the Dominant Culturable N₂-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere

Atefeh Varmazyari, Ramazan Çakmakçı

Published in Frontiers in Environmental Microbiology (Volume 4, Issue 2)

Received: 30 December 2017 Accepted: 24 February 2018 Published: 21 March 2018

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Abstract

The purpose of this study was to investigate the diversity of cultivable N₂-fixing and P-solubilizing bacteria originated from 167 rhizospheric acidic soils samples of tea. Based on the fatty acid methyl ester profiles, 34 bacterial genera were identified with a similarity index of >0.3, but 69.2% of the identified isolates belonged to five genera: Bacillus, Pseudomonas, Paenibacillus, Stenotrophomonas and Arthrobacter. Among the 263 bacterial strains, 213 strains exhibited N₂-fixing activity and 159 were efficient in phosphate solubilisation; 134 strains were efficient in N₂-fixation and P-solubilisation. Most of the N₂-fixing and P-solubilizing bacteria isolated were Gram-positive (59.3 and 52.8%), and Gram-negative constituted only 40.7 and 47.2%. A total of 102 dominant strains were characterized by carbon sources using BIOLOG^M GN2 and GP2 plates. B. pumilus, B. subtilis, B. licheniformis, B. laevolacticus, P. fluorescens, P. putida, S. maltophilia and B. megaterium were the most frequent P-solubilizing and N₂-fixing species in the tea rhizosphere soils. Utilization of high variety of C-sources by the N₂-fixing and P-solubilizing acid tolerant strains may play an important role in adapting to a variety of crop plants, and thus potentially beneficial to the growth of tea plants in that specific acidic ecosystem.

Published in	Frontiers in Environmental Microbiology (Volume 4, Issue 2)
DOI	10.11648/j.fem.20180402.12
Page(s)	45-54
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Tea, Biodiversity, Plant Growth-Promoting Rhizobacteria, Biology, Carbon Source Utilization, Acid Tolerant Strains

References

[1]	Hartman, W. H., Richardson, C. J., Vilgalys, R. and Bruland, G. L. 2008. Environmental and anthropogenic controls over bacterial communities in wetland soils. Proc. Natl. Acad. Sci. USA 105:17842-17847.
[2]	Trivedi, P., Spann, T. and Wang, N. 2011. Isolation and characterization of beneficial bacteria associated with citrus roots in Florida. Microb. Ecol. 62:324-336.
[3]	Beneduzi, A., Moreira, F., Costa, P. B., Vargas, L. K., Lisboa, B. B., Favreto, R., Baldani, J. I. and Passaglia L. M. P. 2013. Diversity and plant growth promoting evaluation abilities of bacteria isolated from sugarcane cultivated in the South of Brazil. Appl. Soil Ecol. 63:94-104.
[4]	Farina, R., Beneduzi, A., Ambrosini, A., de Campos, S. B., Lisboa, B. B., Wendischc, V., Vargas, L. K. and Passaglia, L. M. P. 2012. Diversity of plant growth-promoting rhizobacteria communities associated with the stages of canola growth. Appl. Soil Ecol. 55:44-52.
[5]	Berg, G. and Smalla, K. 2009. Plant species and soil type cooperatively shape the structure and function of microbial communities in the rhizosphere. FEMS Microbiol. Ecol. 68:1–13.
[6]	Ambrosini A., Beneduzi, A., Stefanski, T., Pinheiro, F. G., Vargas, L. K. and Passaglia, L. M. P. 2012. Screening of plant growth promoting rhizobacteria isolated from sunflower (Helianthus annuus L.). Plant Soil 356:245–264.
[7]	Han, W. Y., Ma, L. F., Shi, Y. Z., Ruan, J. Y. and Kemmitt, S. J. 2008. Nitrogen release dynamics and transformation of slow release fertiliser products and their effects on tea yield and quality. J. Sci. Food Agr. 88:839-846.
[8]	Liu, Z., Yang, J., Yang, Z. and Zou, J. 2012. Effects of rainfall and fertilizer types on nitrogen and phosphorus concentrations in surface runoff from subtropical tea fields in Zhejiang, China. Nutr. Cycl. Agroecosyst. 93:297-307.
[9]	Xue, D., Yao, H. and Huang, C. Y. 2006. Microbial biomass, N mineralization and nitrification, enzyme activities, and microbial community diversity in tea orchard soils. Plant Soil 288:319-331.
[10]	Chen, Y. P., Rekha, P. D., Arun, A. B., Shen, F. T., Lai, W.-A. and Young, C. C. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl. Soil Ecol. 34:33-41.
[11]	Çakmakçı, R., Dönmez, M. F., Ertürk, Y., Erat, M., Haznedar, A. and Sekban, R. 2010. Diversity and metabolic potential of culturable bacteria from the rhizosphere of Turkish tea grown in acidic soils. Plant Soil 332:299-318.
[12]	Garland, J. L. and Mills, A. L. 1991. Classification and characterisation of heterotrophic microbial communities on the basis of patterns of community-level-sole-carbon-source-utilization. Appl. Environ. Microbiol. 57:2351–2359.
[13]	Pikovskaya, R. E. 1948. Mobilization of phosphates in soil in connection with vital activities of some microbial species. Microbiologia 17:362-370.
[14]	Mehta, S. and Nautiyal, C. S. 2001. An efficient method for qualitative screening of phosphate-solubilizing bacteria. Curr. Microbiol. 43:51–56.
[15]	Döbereiner, J. 1988. Isolation and identification of root associated diazotrophs. Plant Soil 110:207–212.
[16]	Rau, N., Mishra, V., Sharma, M., Das, M. K., Ahaluwalia, K. and Sharma, R. S. 2009. Evaluation of functional diversity in rhizobacterial taxa of a wild grass (Saccharum ravennae) colonizing abandoned fly ash dumps in Delhi urban ecosystem. Soil Biol. Biochem. 41:813-821.
[17]	Hardy, R. W. F., Holsten, R. D., Jackson, E. K. and Burns, R. C. 1968. The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation. Plant Physiol. 43:1185–1207.
[18]	Sood, A., Sharma, S., Kumar, V. and Thakur, R. L. 2008. Established and abandoned tea (Camillia sinensis L.) rhizosphere: dominant bacteria and their antagonism. Pol. J. Microbiol. 57:71–76.
[19]	Bafana, A. 2013. Diversity and metabolic potential of culturable root-associated bacteria from Origanum vulgare in sub-Himalayan region. World J. Microbiol. Biotechnol. 29:63-74.
[20]	Pandey, A., Singh, S. and Palni, L. M. S. 2013. Microbial inoculants to support tea industry in India. Indian J. Biotechnol. 12:13-19.
[21]	Borsodi, A. K., Makk, J., Rusznyák, A., Vajna, B., Taba, G. and Márialigeti, K. 2007. Phenotypic characterization and molecular taxonomic studies on Bacillus and related isolates from Phragmites australis periphyton. Aquat. Bot. 86:243–252.
[22]	Poonguzhali, S., Madhaiyan, M. and Sa, T. 2006. Cultivation-dependent characterization of rhizobacterial communities from field grown Chinese cabbage Brassica campestris ssp pekinensis and screening of traits for potential plant growth promotion. Plant Soil 286:167-180.
[23]	Karagöz, K., Ates, F., Karagöz, H., Kotan, R. and Çakmakçı, R. 2012. Characterization of plant growth-promoting traits of bacteria isolated from the rhizosphere of grapevine grown in alkaline and acidic soils. Eur. J. Soil Biol. 50:144-150.
[24]	Perez, E., Sulbaran, M., Ball, M. M. and Yarzabal, L. A. 2007. Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region. Soil Biol. Biochem. 39:2905-2914.
[25]	Hariprasad, P. and Niranjana, S. R. 2009. Isolation and characterization of phosphate solubilizing rhizobacteria to improve plant health of tomato. Plant Soil 316:13-24.
[26]	Subramanian, J. and Satyan, K. 2014. Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping. Chilean J. Agric. Res. 74:319-325.
[27]	Navarro-Noya, Y. E., Hernández-Mendoza, E., Morales-Jiménez, J., Jan-Roblero, J., Martínez-Romero, E. and Hernández-Rodríguez, C. 2012. Isolation and characterization of nitrogen fixing heterotrophic bacteria from the rhizosphere of pioneer plants growing on mine tailings. Appl. Soil Ecol. 62:52-60.
[28]	Çakmakçı, R., 2016. Screening of multi-trait rhizobacteria for improving the growth, enzyme activities, and nutrient uptake of tea (Camellia sinensis). Commun. Soil Sci. Plant Anal. 47 (13-14): 1680-1690.
[29]	Jha, B., Thakur, M. C., Gontia, I., Albrecht, V., Stoffels, M., Schmid, M. and Hartmann, A. 2009. Isolation, partial identification and application of diazotrophic rhizobacterial from traditional Indian rice cultivars. Eur. J. Soil Biol. 45:62–72.
[30]	Kumar, K., Amaresan, N., Bhagat, S., Madhuri, K. and Srivastava, R. C. 2011. Isolation and characterization of rhizobacteria associated with coastal agricultural ecosystem of rhizosphere soils of cultivated vegetable crops. World J. Microbiol. Biotechnol. 27:1625-1632.

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APA Style

Atefeh Varmazyari, Ramazan Çakmakçı. (2018). Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere. Frontiers in Environmental Microbiology, 4(2), 45-54. https://doi.org/10.11648/j.fem.20180402.12

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ACS Style

Atefeh Varmazyari; Ramazan Çakmakçı. Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere. Front. Environ. Microbiol. 2018, 4(2), 45-54. doi: 10.11648/j.fem.20180402.12

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AMA Style

Atefeh Varmazyari, Ramazan Çakmakçı. Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere. Front Environ Microbiol. 2018;4(2):45-54. doi: 10.11648/j.fem.20180402.12

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@article{10.11648/j.fem.20180402.12,
  author = {Atefeh Varmazyari and Ramazan Çakmakçı},
  title = {Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere},
  journal = {Frontiers in Environmental Microbiology},
  volume = {4},
  number = {2},
  pages = {45-54},
  doi = {10.11648/j.fem.20180402.12},
  url = {https://doi.org/10.11648/j.fem.20180402.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20180402.12},
  abstract = {The purpose of this study was to investigate the diversity of cultivable N2-fixing and P-solubilizing bacteria originated from 167 rhizospheric acidic soils samples of tea. Based on the fatty acid methyl ester profiles, 34 bacterial genera were identified with a similarity index of >0.3, but 69.2% of the identified isolates belonged to five genera: Bacillus, Pseudomonas, Paenibacillus, Stenotrophomonas and Arthrobacter. Among the 263 bacterial strains, 213 strains exhibited N2-fixing activity and 159 were efficient in phosphate solubilisation; 134 strains were efficient in N2-fixation and P-solubilisation. Most of the N2-fixing and P-solubilizing bacteria isolated were Gram-positive (59.3 and 52.8%), and Gram-negative constituted only 40.7 and 47.2%. A total of 102 dominant strains were characterized by carbon sources using BIOLOGM GN2 and GP2 plates. B. pumilus, B. subtilis, B. licheniformis, B. laevolacticus, P. fluorescens, P. putida, S. maltophilia and B. megaterium were the most frequent P-solubilizing and N2-fixing species in the tea rhizosphere soils. Utilization of high variety of C-sources by the N2-fixing and P-solubilizing acid tolerant strains may play an important role in adapting to a variety of crop plants, and thus potentially beneficial to the growth of tea plants in that specific acidic ecosystem.},
 year = {2018}
}

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TY  - JOUR
T1  - Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere
AU  - Atefeh Varmazyari
AU  - Ramazan Çakmakçı
Y1  - 2018/03/21
PY  - 2018
N1  - https://doi.org/10.11648/j.fem.20180402.12
DO  - 10.11648/j.fem.20180402.12
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 45
EP  - 54
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20180402.12
AB  - The purpose of this study was to investigate the diversity of cultivable N2-fixing and P-solubilizing bacteria originated from 167 rhizospheric acidic soils samples of tea. Based on the fatty acid methyl ester profiles, 34 bacterial genera were identified with a similarity index of >0.3, but 69.2% of the identified isolates belonged to five genera: Bacillus, Pseudomonas, Paenibacillus, Stenotrophomonas and Arthrobacter. Among the 263 bacterial strains, 213 strains exhibited N2-fixing activity and 159 were efficient in phosphate solubilisation; 134 strains were efficient in N2-fixation and P-solubilisation. Most of the N2-fixing and P-solubilizing bacteria isolated were Gram-positive (59.3 and 52.8%), and Gram-negative constituted only 40.7 and 47.2%. A total of 102 dominant strains were characterized by carbon sources using BIOLOGM GN2 and GP2 plates. B. pumilus, B. subtilis, B. licheniformis, B. laevolacticus, P. fluorescens, P. putida, S. maltophilia and B. megaterium were the most frequent P-solubilizing and N2-fixing species in the tea rhizosphere soils. Utilization of high variety of C-sources by the N2-fixing and P-solubilizing acid tolerant strains may play an important role in adapting to a variety of crop plants, and thus potentially beneficial to the growth of tea plants in that specific acidic ecosystem.
VL  - 4
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Author Information

Atefeh Varmazyari

Department of Agronomy, Faculty of Agriculture, Atatürk University, Erzurum, Turkey
Ramazan Çakmakçı

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APA Style

Atefeh Varmazyari, Ramazan Çakmakçı. (2018). Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere. Frontiers in Environmental Microbiology, 4(2), 45-54. https://doi.org/10.11648/j.fem.20180402.12

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ACS Style

Atefeh Varmazyari; Ramazan Çakmakçı. Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere. Front. Environ. Microbiol. 2018, 4(2), 45-54. doi: 10.11648/j.fem.20180402.12

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AMA Style

Atefeh Varmazyari, Ramazan Çakmakçı. Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere. Front Environ Microbiol. 2018;4(2):45-54. doi: 10.11648/j.fem.20180402.12

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@article{10.11648/j.fem.20180402.12,
  author = {Atefeh Varmazyari and Ramazan Çakmakçı},
  title = {Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere},
  journal = {Frontiers in Environmental Microbiology},
  volume = {4},
  number = {2},
  pages = {45-54},
  doi = {10.11648/j.fem.20180402.12},
  url = {https://doi.org/10.11648/j.fem.20180402.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20180402.12},
  abstract = {The purpose of this study was to investigate the diversity of cultivable N2-fixing and P-solubilizing bacteria originated from 167 rhizospheric acidic soils samples of tea. Based on the fatty acid methyl ester profiles, 34 bacterial genera were identified with a similarity index of >0.3, but 69.2% of the identified isolates belonged to five genera: Bacillus, Pseudomonas, Paenibacillus, Stenotrophomonas and Arthrobacter. Among the 263 bacterial strains, 213 strains exhibited N2-fixing activity and 159 were efficient in phosphate solubilisation; 134 strains were efficient in N2-fixation and P-solubilisation. Most of the N2-fixing and P-solubilizing bacteria isolated were Gram-positive (59.3 and 52.8%), and Gram-negative constituted only 40.7 and 47.2%. A total of 102 dominant strains were characterized by carbon sources using BIOLOGM GN2 and GP2 plates. B. pumilus, B. subtilis, B. licheniformis, B. laevolacticus, P. fluorescens, P. putida, S. maltophilia and B. megaterium were the most frequent P-solubilizing and N2-fixing species in the tea rhizosphere soils. Utilization of high variety of C-sources by the N2-fixing and P-solubilizing acid tolerant strains may play an important role in adapting to a variety of crop plants, and thus potentially beneficial to the growth of tea plants in that specific acidic ecosystem.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere
AU  - Atefeh Varmazyari
AU  - Ramazan Çakmakçı
Y1  - 2018/03/21
PY  - 2018
N1  - https://doi.org/10.11648/j.fem.20180402.12
DO  - 10.11648/j.fem.20180402.12
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 45
EP  - 54
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20180402.12
AB  - The purpose of this study was to investigate the diversity of cultivable N2-fixing and P-solubilizing bacteria originated from 167 rhizospheric acidic soils samples of tea. Based on the fatty acid methyl ester profiles, 34 bacterial genera were identified with a similarity index of >0.3, but 69.2% of the identified isolates belonged to five genera: Bacillus, Pseudomonas, Paenibacillus, Stenotrophomonas and Arthrobacter. Among the 263 bacterial strains, 213 strains exhibited N2-fixing activity and 159 were efficient in phosphate solubilisation; 134 strains were efficient in N2-fixation and P-solubilisation. Most of the N2-fixing and P-solubilizing bacteria isolated were Gram-positive (59.3 and 52.8%), and Gram-negative constituted only 40.7 and 47.2%. A total of 102 dominant strains were characterized by carbon sources using BIOLOGM GN2 and GP2 plates. B. pumilus, B. subtilis, B. licheniformis, B. laevolacticus, P. fluorescens, P. putida, S. maltophilia and B. megaterium were the most frequent P-solubilizing and N2-fixing species in the tea rhizosphere soils. Utilization of high variety of C-sources by the N2-fixing and P-solubilizing acid tolerant strains may play an important role in adapting to a variety of crop plants, and thus potentially beneficial to the growth of tea plants in that specific acidic ecosystem.
VL  - 4
IS  - 2
ER  -

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