Bacterial resistance to antibiotics has become a global problem impacted partly by use of antibiotics in various environments. New antimicrobial agents are therefore urgently needed to overcome this problem. This study was carried out to investigate the tetracycline (TC) and ampicillin (AMP) resistant bacteria in wastewater environments of Rajshahi Metropolitan City, Bangladesh. In this investigation ethanol and acetone extracts of Dryopteris sp. (leaves), Pteris vittata L. (leaves) and Nigella sativa L. (seeds) were examined for their antibacterial activity. A total of 34 TC and AMP resistant bacteria were isolated from wastewater sources. Among them, a random selection of eight strains (three gram negative and 5 gram positive) was used for this study. The zone of inhibition for Dryopteris sp. leaves ranged from 6-11 mm and 6-12 mm and for Pteris vittata L. leaf extract, from 6-12 mm and 6-13 mm within ethanol and acetone extracts, respectively. Acetone extract of Nigella sativa L. seeds at the concentration of 2500 µg/ml had a marked sensitivity (7-18 mm) towards three tested gram positive bacteria in this study. The results of the present work provide essential baseline information for the use of the studied plants in the fight against drug resistant phenotypes.
| Published in | Frontiers in Environmental Microbiology (Volume 1, Issue 2) |
| DOI | 10.11648/j.fem.20150102.14 |
| Page(s) | 27-31 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Antibacterial Activity, Pteridophytic Plants, Nigella sativa, Leaves Extracts, Antibiotic Resistant Bacteria
| [1] | F. Baquero, J. L. Martinez and R. Canton. Antibiotics and antibiotic resistance in water environments. Curr. Opin. Biotechnol., 19: 260–265, 2008. |
| [2] | M. P. Schlüsener and K. Bester. Persistence of antibiotics such as macrolides, tiamulin and salinomycin in soil. Environ. Pollut., 143: 565–571, 2006. |
| [3] | F. A. Neela, L. Nonaka and S. Suzuki. The diversity of multi-drug resistance profiles in tetracycline-resistant Vibrio species isolated from coastal sediment and seawater. J. Microbiol., 45: 64-68, 2007. |
| [4] | M. H. Rahman, L. Nonaka, R. Tago and S. Suzuki. Occurrence of two genotypes of tetracycline (TC) resistance gene tet (M) in the TC-resistant bacteria in marine sediments of Japan. Environ. Sci. Technol., 42: 5055–5061, 2008b. |
| [5] | F. A. Neela, L. Nonaka, M. H. Rahman and S. Suzuki. Transfer of chromosomal encoded tetracycline resistance gene tet (M) from marine bacteria to Escherichia coli and Enterococcus faecalis. World J. Microbiol.Biotechnol., 25: 1095-1101, 2009. |
| [6] | F. ANeela, M. A. Rahman, M. N. A. Banu, M. H. Rahman, H. Ohta and M. F. Alam. Occurrence of two antibiotic resistant bacteria in aquatic environment associated with shrimp farming in Bangladesh. Bangladesh J. Bot., 41: 197-200, 2012. |
| [7] | A. Bond. Wastewater a source of antibiotic-resistant bacteria: study. Clin. Infec. Dis., online. source: bit.ly/1ksBD98, 2014. |
| [8] | F. Moges, M. Endris, Y. Belyhun and W. Worku. Isolation and characterization of multiple drug resistance bacterial pathogens from waste waterin hospital and non-hospital environments, Northwest Ethiopia. BMC Res. Notes, 7:215- 221, 2014. |
| [9] | D. E. Djeussi, J. A. K. Noumedem, J. A. Seukep, A. G. Fankam, S. B. Tankeo, A. H. L. Nkuete and V. Kuete. Antibacterial activities of selected edible plants extracts against multidrug-resistant Gram-negative bacteria. BMC Complementary Altern. Med., 13:164-172, 2013. |
| [10] | H. Chandarana, S. Baluja and S. V. Chanda. Comparison of antibacterial activities of selected species of zingiberaceae family and some synthetic compounds. Turk. J. Biol., 29:83-97, 2005. |
| [11] | B. Adriana, A. N. M. Almodóvar1, C. T. Pereira1 and T. A. Mariângela. Antimicrobial efficacy of Curcuma zedoaria extract as assessed by linear regression compared with commercial mouthrinses. Braz. J. Microbiol., 38: 440-445, 2007. |
| [12] | A. Aktar, F. A. Neela, M. S. I. Khan, M. S. Islam and M. F. Alam. Screening of ethanol, petroleum ether and chloroform extracts of medicinal plants Lawsonia inermis L. and Mimosa pudica L. for antibacterial activity. Indian J. Pharma. Sci., 72: 388-392, 2010. |
| [13] | M. S. I. Khan, F. A. Neela, A. Aktar, M. M. Rahman and M. F. Alam. Antibacterial activity of Achyranthes aspera L. - an in vitro study. J. Environ. Sci.&Natural Resources, 2: 45-48, 2009. |
| [14] | M. T. Salman, R. A. Khan and I. Shukla. Antibacterial activity of Nigella sativa Linn. seed oil against multidrug-resistant bacteria from clinical isolates. Natur. Pro. Rad., 7: 10-14, 2008. |
| [15] | D. Rani, P. B. Khare and P. K. Dantu. In vitro antibacterial and antifungal properties of aqueous and non-aqueous frond extracts of Psilotum nudum, Nephrolepis biserrata and Nephrolepis cordifolia. Indian J. Pharm. Sci., 72: 818–822, 2010. |
| [16] | C. Walsh. Antibiotics: actions, origins, resistance. ASM Press,Washington, DC, 2003. |
| [17] | J. G. Holt and D. H. Bergey. Bergey's Manual of Systematic Bacteriology. 9th rev. ed. (1 Oct. 1993) Lippincott Williams and Wilkins. The University of Michigan, USA, 1923. |
| [18] | M. Chandrasekaran and V. Venkatesalu. Antibacterial and antifungal activity of Syzygium jambolanum seeds. J. Ethnopharmacol., 91:105-8, 2004. |
| [19] | A. W. Bauer, W. M. Kirby, S. C. Sherries and M. Turk. Antibiotic susceptibility testing by a standard single disc method. Am. J. Clinic. Pathol.,45:493-6, 1996. |
| [20] | P. Parihar, L. Parihar and A. Bohra. In vitro antibacterial activity of fronds (leaves) of some important pteridophytes. J. Microbiol. Antimicrob., 2: 19-22, 2010. |
| [21] | S. Chandra. Ferns of India: International Book Distributors, Dehradun, India, 2000. |
| [22] | M. Kumar, M. Ramesh and S. Sequiera. Medicinal pteridophytes of Kerala, South India. Ind. Fern J.,20: 1-28, 2003. |
| [23] | A. Sharma and V. K. Patel. In vitro screening of the antibacterial activity and identification of bioactive compounds from plants against selected vibrio spp. pathogens. Turk. J. Biol., 33: 137-144, 2009. |
| [24] | A. Nagi, N. S. Mariana, F. Z. Hana and A. Rasedee. Extraction of essential oil from Nigella sativa using superfacial carbondioxide: Study of antibacterial activity. Am. J.Pharmacol. Toxicol.,3: 225-228, 2008. |
| [25] | Abu-shanab, G. Adwan, D. Abu-Safiya, N. Jarrar and K. Adwan. Antibacterial activities of some plant extracts utilized in popular medicine in Palestine. Turk.J.Biol. 28: 99-102, 2004. |
| [26] | N. A. Hasan, M. Z. Mohd. Zaini Nawahwi and H. B. Malek. Antimicrobial Activity of Nigella sativa Seed Extract. Sains Malaysiana, 42(2): 143–147, 2013. |
APA Style
Farzana Ashrafi Neela, Rokshana Parvin, Niren Chandra Mahato, Md. Momtaz Uddin, Lipika Ghosh, et al. (2015). Antibacterial Activity of Pteridophytes and Nigella sativa Against Antibiotic Resistant Bacteria Isolated from Wastewater Environment. Frontiers in Environmental Microbiology, 1(2), 27-31. https://doi.org/10.11648/j.fem.20150102.14
ACS Style
Farzana Ashrafi Neela; Rokshana Parvin; Niren Chandra Mahato; Md. Momtaz Uddin; Lipika Ghosh, et al. Antibacterial Activity of Pteridophytes and Nigella sativa Against Antibiotic Resistant Bacteria Isolated from Wastewater Environment. Front. Environ. Microbiol. 2015, 1(2), 27-31. doi: 10.11648/j.fem.20150102.14
AMA Style
Farzana Ashrafi Neela, Rokshana Parvin, Niren Chandra Mahato, Md. Momtaz Uddin, Lipika Ghosh, et al. Antibacterial Activity of Pteridophytes and Nigella sativa Against Antibiotic Resistant Bacteria Isolated from Wastewater Environment. Front Environ Microbiol. 2015;1(2):27-31. doi: 10.11648/j.fem.20150102.14
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.fem.20150102.14,
author = {Farzana Ashrafi Neela and Rokshana Parvin and Niren Chandra Mahato and Md. Momtaz Uddin and Lipika Ghosh and Most. Ferdousi Begum},
title = {Antibacterial Activity of Pteridophytes and Nigella sativa Against Antibiotic Resistant Bacteria Isolated from Wastewater Environment},
journal = {Frontiers in Environmental Microbiology},
volume = {1},
number = {2},
pages = {27-31},
doi = {10.11648/j.fem.20150102.14},
url = {https://doi.org/10.11648/j.fem.20150102.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20150102.14},
abstract = {Bacterial resistance to antibiotics has become a global problem impacted partly by use of antibiotics in various environments. New antimicrobial agents are therefore urgently needed to overcome this problem. This study was carried out to investigate the tetracycline (TC) and ampicillin (AMP) resistant bacteria in wastewater environments of Rajshahi Metropolitan City, Bangladesh. In this investigation ethanol and acetone extracts of Dryopteris sp. (leaves), Pteris vittata L. (leaves) and Nigella sativa L. (seeds) were examined for their antibacterial activity. A total of 34 TC and AMP resistant bacteria were isolated from wastewater sources. Among them, a random selection of eight strains (three gram negative and 5 gram positive) was used for this study. The zone of inhibition for Dryopteris sp. leaves ranged from 6-11 mm and 6-12 mm and for Pteris vittata L. leaf extract, from 6-12 mm and 6-13 mm within ethanol and acetone extracts, respectively. Acetone extract of Nigella sativa L. seeds at the concentration of 2500 µg/ml had a marked sensitivity (7-18 mm) towards three tested gram positive bacteria in this study. The results of the present work provide essential baseline information for the use of the studied plants in the fight against drug resistant phenotypes.},
year = {2015}
}
TY - JOUR T1 - Antibacterial Activity of Pteridophytes and Nigella sativa Against Antibiotic Resistant Bacteria Isolated from Wastewater Environment AU - Farzana Ashrafi Neela AU - Rokshana Parvin AU - Niren Chandra Mahato AU - Md. Momtaz Uddin AU - Lipika Ghosh AU - Most. Ferdousi Begum Y1 - 2015/09/11 PY - 2015 N1 - https://doi.org/10.11648/j.fem.20150102.14 DO - 10.11648/j.fem.20150102.14 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 27 EP - 31 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20150102.14 AB - Bacterial resistance to antibiotics has become a global problem impacted partly by use of antibiotics in various environments. New antimicrobial agents are therefore urgently needed to overcome this problem. This study was carried out to investigate the tetracycline (TC) and ampicillin (AMP) resistant bacteria in wastewater environments of Rajshahi Metropolitan City, Bangladesh. In this investigation ethanol and acetone extracts of Dryopteris sp. (leaves), Pteris vittata L. (leaves) and Nigella sativa L. (seeds) were examined for their antibacterial activity. A total of 34 TC and AMP resistant bacteria were isolated from wastewater sources. Among them, a random selection of eight strains (three gram negative and 5 gram positive) was used for this study. The zone of inhibition for Dryopteris sp. leaves ranged from 6-11 mm and 6-12 mm and for Pteris vittata L. leaf extract, from 6-12 mm and 6-13 mm within ethanol and acetone extracts, respectively. Acetone extract of Nigella sativa L. seeds at the concentration of 2500 µg/ml had a marked sensitivity (7-18 mm) towards three tested gram positive bacteria in this study. The results of the present work provide essential baseline information for the use of the studied plants in the fight against drug resistant phenotypes. VL - 1 IS - 2 ER -
Information
Email: