Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India

Chandraval Dutta; Ashis Kumar Panigrahi; Chandan Sengupta

doi:doi:10.11648/j.fem.20150102.11

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Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India

Chandraval Dutta, Ashis Kumar Panigrahi, Chandan Sengupta

Published in Frontiers in Environmental Microbiology (Volume 1, Issue 2)

Received: 29 July 2015 Accepted: 10 August 2015 Published: 19 August 2015

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Abstract

The objective of this study was to determine the microbiological quality and prevalence of pathogenic bacteria viz. Vibrio cholerae, E. coli, Salmonella spp. in fish and shellfish from domestic markets of West Bengal, India. Fresh Penaeus monodon, Macrobrachium rozenbergii, Labeo rohita, Catla catla, Cirhinnus mrigala were examined for total bacterial and faecal coliform loads and presence of Vibrio cholerae, E. coli, Salmonella spp by culture methods. While the total plate counts of bacteria were within the acceptable or marginally acceptable limits for most samples, fishes were contaminated with faecal coliforms indicating poor sanitary hygienic condition. The specific pathogens like Vibrio cholerae and Salmonella spp were also detected in fish and shellfish samples. Antibiotic sensitivity tests showed that the pathogens were resistant to antibiotics raising serious public health concerns.

Published in	Frontiers in Environmental Microbiology (Volume 1, Issue 2)
DOI	10.11648/j.fem.20150102.11
Page(s)	14-18
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Microbiological Quality, Fish and Shellfish, Vibrio cholerae, E. coli, Salmonella spp, Antibiotic Sensitivity, Bacterial Load

References

[1]	M.L. Heinitz, R.D. Ruble, D.E.Wagner, and S.R.Tatini,, Incidence of Salmonellain fish and seafood, J. Food Prot. 2000 pp 63 579-592.
[2]	A.S. Kamat, J.R.M Bandekar,, S. Karani, R. Jadhav, A. Shasidhar, S Kakatkar, K. Pingulkar, N. Ghadge, S.B.R.Warrier, V. VenugopalMicrobiological quality of some major fishery products exported from India. 2002 Proceed. IAEA Research Coordinators Meeting.
[3]	M.Pandian, A., Kulkarni, S., Pawar and S.B. DesaiPathway for microbial pathogens in fish at fish landing centres of Mumbai, Abstract, First Indian Fisheries Science Congress, 2000. Chandigarh, India, 21-23 September.
[4]	I. Potasman, A. Paz, M. Odeh, Infectious outbreaks associated with bivalve shellfish consumption: a worldwide perspective. Clin. Infect. Dis.2002 35 pp 921 – 928.
[5]	F.E. Ahmed(ed), Seafood Safety committee on the Evaluation of the safety of fishery products. Food and Nutritional Board, Institute of Medicine, Washington, National Academy Press, 1991. pp 474.
[6]	S.K. Manna, R. Das and C. MannaMicrobiological quality of finfish and shellfish with special reference to shiga toxin –producing Escherichia coli O157, J. Food Science, 2008 73.(6) pp M283-286.
[7]	E.L.Elliot, C.A.Kaysner, L. Jackson, M.L.Tamplin, Vibrio cholerae, V. parahaemolyticus, V. vulnificus, and other Vibrio spp. Bacterial Analytical Manual, 8th ed. Association of Official Analytical Chemists, Arlington, VA. 1995 pp. 9.01 – 9.27.
[8]	G.B.Nair, B.L. Sarkar, S.P. De,, M.K. Chakraborty, R.K Bhadra and S.C. PalEcology of Vibrio cholerae in the freshwater environment of Calcutta, India, Microb.E.col 1988 15 pp 203-216.
[9]	J.D. Oliver, & J.B. Kaper, in: M.P. Doyle, L.R. Beuchat and T.J.Montville (eds). Food Microbiology: Fundamentals and Frontiers. Washington, D.C., United States of America: American Society for Microbiology Press.1997. Vibrio species. pp. 228–264.
[10]	T.S.G.Iyer Faecal Indicator Bacteria in Fish In Quality Assurance in Seafood Processing2005. Central Institute of Fisheries Technology, Matsyapuri, Cochin.
[11]	I.Karunasagar, B.B. Nayak, I. Karunasagar Rapid detection of Vibrio parahaemolyticus from fish by polymerase chain reaction (PCR). In: Flegel T, MacRae IH (eds) Diseases in Asian aquaculture, 3rd edn. Asian Fisheries Society, Manila, 1997. pp 119– 122.
[12]	P. Baumann, A.Furniss, and J. Lee: Genus I. Vibrio. In Bergey’s manual of systematicbacteriology.CurrentMicrobiology.1984pp518-538. DOI:10.1007/PL00006762
[13]	R.R.Colwell, Global climate and infectious disease: The cholera paradigm. Science, 1996. 274: 2025-2031. DOI: 10.1126/science.274.5295.2025.
[14]	A. S.Kamat, J. R. M. Bandekar, S. Karani, R.Jadhav, A. Shashidhar, S. Kakatkar, K.Pingulkar, N.Ghadge, S. B. R. Warrier and V. Venugopal, Microbiological quality of some major fishery products Exported from India. Determination of human pathogen profiles in food by quality assured microbial assays. Proceedings of a final Research Coordination Meeting held in Mexico City, 2005.Mexico, 22–26 July 2002.
[15]	B. P. Upadhyay, F.Utrarachkij, J.Thongshoob, Y. Mahakunkijcharoen, N. Wongchinda, O.Suthienkul,andS.Khusmith, Detection Of Salmonella inva Gene in Shrimp Enrichment Culture By Polymerase Chain Reaction. Southeast Asian J. Trop Med. Public Healt. 2010.41: 2 pp 426-435.
[16]	H.H.Huss andL.GramCharacterization of Hazards in Seafood Assurance of Seafood Quality FAO Fisheries Technical Paper 2004. 444pp227.
[17]	Bacteriological Analytical Manual, 8th ed. (2001) U.S. Food and Drug Administration.Arlington, VA: Association of Official Analytical Chemists. www.fda.gov/ Food/Food Science Research
[18]	A. W. Bauer, W.M.M. Kirby, J.C. Sherris and M.Truck, Antibiotic susceptibility testing by a standardized single disc method. Am.J.Clin.Pathol. 1966. 45 pp 493-496.
[19]	ICMSF. Microorganisms in food. 2. Sampling of microbiological analysis: principles and specific applications. 2nd ed. Buffalo, N.Y:Univ.of Toronto 1986.
[20]	Centre for Disease Control and PreventionSurveillance for food borne disease outbreaks- United States,1993-97, Morb. Mortalwkly. Rep. Surveill. Summ 2000.49 pp 1-5.
[21]	S. Francis, J. Rowland, K. Rattenbury, D. Powell, W.N.Rogers, L. Ward, and S.R. ParmerEpidemiol. Infect1989 103:445.
[22]	H.S.Kumar, A. Parvathi, I. Karunasagar and I.Karunasagar.Prevalence and antibiotic resistance in Escherichia coli in tropical Seafood. World Journal of Microbiology & Biotechnology 2005 21 pp 619–623.
[23]	E.E. Geldreich Coliforms: a new beginning to an old problem. In Coliforms and E. coli: Problem or Solution, eds. Kay, D. &Fricker, C., 1997 pp. 3–11. Cambridge: The Royal Society of Chemistry.ISBN 0-85404-771-9.
[24]	M.J. Albert,Vibrio cholerae O139 Bengal. J. Clin. Microbiol. 1994 32 pp 2345–2349.
[25]	R.R.Colwell,, Global Climate and infection disease: the cholera paradigm, Science 1996. 274 pp 2025-2031.
[26]	S.M. Faruque, M.J. Albert, J.J. Mekalanos Epidemiology, genetics,and ecoology of toxigenic Vibrio cholerae. Microbiol. Mol. Biol. Rev. 1998. 62 pp 1301–1314.
[27]	A. Huq, R.R. Colwell, R. Rahaman, A. Ali, M.A. Chowdhury, S.Parveen, D.A. Sack and E. Russek-cohen. Detction of Vibrio cholerae O1 in the aquatic environment by fluorescent- monoclonal antibody and culture methods: Applied Environment. Microbiol1990 56: 2370-2373.
[28]	T. Ramamurthi, S. Garg, R. Sharma, S.K. Bhattacharyay, G.B. Nair, T. Shimada, T. Takeda, T. Karasawa, H. Kurazano, A. PAL, Y. Takeda. Emergence of a novel strain of Vibrio cholerae with epidemic potential in Southern and Eastern India Lancet. 1993.341 pp703–704.
[29]	B.Das S.C, Das, S.K. Manna and P. Sarkar Antimicrobial susceptibility of Vibrio parahaemolyticus isolated from fish and shellfish in Kolkata, J.Inland. Fish.Soc. India, 2009. 41(1) pp73-76.
[30]	W.A. Khan, J.S. Hossain, and N.S. Uddin, Isolation and Determination of Antibiotic Susceptibility of Vibrio parahaemolyticus from shrimp at Khulna Region of Bangladesh Research. J. of Microbiol 2007. 2(3) pp 216-227.
[31]	G. Duffy, O.M. Cloak, M.G. O’Sullivan, A. Guillet, J.J. Sheridan, I.S. Blair and D.A.Mcdowell The incidence and antibiotic resistance profiles of Salmonella spp. On Irish retail meat products, Food Microbiology1999.16 pp 623-631.
[32]	P. Smith, L. Pursell, F. McCormack, A. O'Reilley and M. Hiney. On the significance of bacterial resistance to oxytetracycline in sediments under Norwegian fish farms. Bull. Eur. Assoc. Fish Pathol., 1995. 15 pp 105-106.
[33]	H. Sørum, Antibiotic resistance in aquaculture. Acta. Vet. Scand., Suppl. 1999.92. pp 29-36

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APA Style

Chandraval Dutta, Ashis Kumar Panigrahi, Chandan Sengupta. (2015). Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India. Frontiers in Environmental Microbiology, 1(2), 14-18. https://doi.org/10.11648/j.fem.20150102.11

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ACS Style

Chandraval Dutta; Ashis Kumar Panigrahi; Chandan Sengupta. Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India. Front. Environ. Microbiol. 2015, 1(2), 14-18. doi: 10.11648/j.fem.20150102.11

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AMA Style

Chandraval Dutta, Ashis Kumar Panigrahi, Chandan Sengupta. Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India. Front Environ Microbiol. 2015;1(2):14-18. doi: 10.11648/j.fem.20150102.11

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@article{10.11648/j.fem.20150102.11,
  author = {Chandraval Dutta and Ashis Kumar Panigrahi and Chandan Sengupta},
  title = {Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India},
  journal = {Frontiers in Environmental Microbiology},
  volume = {1},
  number = {2},
  pages = {14-18},
  doi = {10.11648/j.fem.20150102.11},
  url = {https://doi.org/10.11648/j.fem.20150102.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20150102.11},
  abstract = {The objective of this study was to determine the microbiological quality and prevalence of pathogenic bacteria viz. Vibrio cholerae, E. coli, Salmonella spp. in fish and shellfish from domestic markets of West Bengal, India. Fresh Penaeus monodon, Macrobrachium rozenbergii, Labeo rohita, Catla catla, Cirhinnus mrigala were examined for total bacterial and faecal coliform loads and presence of Vibrio cholerae, E. coli, Salmonella spp by culture methods. While the total plate counts of bacteria were within the acceptable or marginally acceptable limits for most samples, fishes were contaminated with faecal coliforms indicating poor sanitary hygienic condition. The specific pathogens like Vibrio cholerae and Salmonella spp were also detected in fish and shellfish samples. Antibiotic sensitivity tests showed that the pathogens were resistant to antibiotics raising serious public health concerns.},
 year = {2015}
}

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TY  - JOUR
T1  - Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India
AU  - Chandraval Dutta
AU  - Ashis Kumar Panigrahi
AU  - Chandan Sengupta
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AB  - The objective of this study was to determine the microbiological quality and prevalence of pathogenic bacteria viz. Vibrio cholerae, E. coli, Salmonella spp. in fish and shellfish from domestic markets of West Bengal, India. Fresh Penaeus monodon, Macrobrachium rozenbergii, Labeo rohita, Catla catla, Cirhinnus mrigala were examined for total bacterial and faecal coliform loads and presence of Vibrio cholerae, E. coli, Salmonella spp by culture methods. While the total plate counts of bacteria were within the acceptable or marginally acceptable limits for most samples, fishes were contaminated with faecal coliforms indicating poor sanitary hygienic condition. The specific pathogens like Vibrio cholerae and Salmonella spp were also detected in fish and shellfish samples. Antibiotic sensitivity tests showed that the pathogens were resistant to antibiotics raising serious public health concerns.
VL  - 1
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Author Information

Chandraval Dutta

Department of Zoology, University of Kalyani, Kalyani,West Bengal, India
Ashis Kumar Panigrahi

Department of Zoology, University of Kalyani, Kalyani,West Bengal, India
Chandan Sengupta

Department of Botany, University of Kalyani, Kalyani, West Bengal, India

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Plain Text BibTeX RIS

APA Style

Chandraval Dutta, Ashis Kumar Panigrahi, Chandan Sengupta. (2015). Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India. Frontiers in Environmental Microbiology, 1(2), 14-18. https://doi.org/10.11648/j.fem.20150102.11

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ACS Style

Chandraval Dutta; Ashis Kumar Panigrahi; Chandan Sengupta. Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India. Front. Environ. Microbiol. 2015, 1(2), 14-18. doi: 10.11648/j.fem.20150102.11

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AMA Style

Chandraval Dutta, Ashis Kumar Panigrahi, Chandan Sengupta. Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India. Front Environ Microbiol. 2015;1(2):14-18. doi: 10.11648/j.fem.20150102.11

Copy | Download

@article{10.11648/j.fem.20150102.11,
  author = {Chandraval Dutta and Ashis Kumar Panigrahi and Chandan Sengupta},
  title = {Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India},
  journal = {Frontiers in Environmental Microbiology},
  volume = {1},
  number = {2},
  pages = {14-18},
  doi = {10.11648/j.fem.20150102.11},
  url = {https://doi.org/10.11648/j.fem.20150102.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20150102.11},
  abstract = {The objective of this study was to determine the microbiological quality and prevalence of pathogenic bacteria viz. Vibrio cholerae, E. coli, Salmonella spp. in fish and shellfish from domestic markets of West Bengal, India. Fresh Penaeus monodon, Macrobrachium rozenbergii, Labeo rohita, Catla catla, Cirhinnus mrigala were examined for total bacterial and faecal coliform loads and presence of Vibrio cholerae, E. coli, Salmonella spp by culture methods. While the total plate counts of bacteria were within the acceptable or marginally acceptable limits for most samples, fishes were contaminated with faecal coliforms indicating poor sanitary hygienic condition. The specific pathogens like Vibrio cholerae and Salmonella spp were also detected in fish and shellfish samples. Antibiotic sensitivity tests showed that the pathogens were resistant to antibiotics raising serious public health concerns.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Prevalence of Pathogenic Bacteria in Finfish and Shellfish Obtained from Domestic Markets of West Bengal, India
AU  - Chandraval Dutta
AU  - Ashis Kumar Panigrahi
AU  - Chandan Sengupta
Y1  - 2015/08/19
PY  - 2015
N1  - https://doi.org/10.11648/j.fem.20150102.11
DO  - 10.11648/j.fem.20150102.11
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 14
EP  - 18
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20150102.11
AB  - The objective of this study was to determine the microbiological quality and prevalence of pathogenic bacteria viz. Vibrio cholerae, E. coli, Salmonella spp. in fish and shellfish from domestic markets of West Bengal, India. Fresh Penaeus monodon, Macrobrachium rozenbergii, Labeo rohita, Catla catla, Cirhinnus mrigala were examined for total bacterial and faecal coliform loads and presence of Vibrio cholerae, E. coli, Salmonella spp by culture methods. While the total plate counts of bacteria were within the acceptable or marginally acceptable limits for most samples, fishes were contaminated with faecal coliforms indicating poor sanitary hygienic condition. The specific pathogens like Vibrio cholerae and Salmonella spp were also detected in fish and shellfish samples. Antibiotic sensitivity tests showed that the pathogens were resistant to antibiotics raising serious public health concerns.
VL  - 1
IS  - 2
ER  -

Copy | Download