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Tensile Strength of Single Collagen Fibrils Isolated from Tendons

Received: 28 November 2016     Accepted: 7 January 2017     Published: 16 February 2017
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Abstract

Tensile failure properties of single collagen fibrils were determined using our original tensile test method. Fibrils were directly isolated from the fascicles of mouse tail tendons. Both the ends of each fibril were wound onto the tips of two microneedles several times using micromanipulators. The fibril and tips were immersed in physiological saline solution. Then, the fibril was stretched to failure by moving the one microneedle. During tensile testing, the fibril was firmly attached to the tips of the microneedles, and broken between the tips with no slippage observed. The diameter of tested 10 fibrils was 410±60 nm (Mean±S.D.). The stress-strain curves of these fibrils were almost linear. Their tensile strength and failure strain were 100±32 MPa and 34±11%, respectively. These values were approximately 480 and 190% of those of the fascicles with the diameter of 81±12 m, respectively.

Published in European Journal of Biophysics (Volume 5, Issue 1)
DOI 10.11648/j.ejb.20170501.11
Page(s) 1-6
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Collagen, Fibril, Tendon, Mechanical Properties, Tensile Strength

References
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    Noritaka Yamamoto. (2017). Tensile Strength of Single Collagen Fibrils Isolated from Tendons. European Journal of Biophysics, 5(1), 1-6. https://doi.org/10.11648/j.ejb.20170501.11

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    Noritaka Yamamoto. Tensile Strength of Single Collagen Fibrils Isolated from Tendons. Eur. J. Biophys. 2017, 5(1), 1-6. doi: 10.11648/j.ejb.20170501.11

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    AMA Style

    Noritaka Yamamoto. Tensile Strength of Single Collagen Fibrils Isolated from Tendons. Eur J Biophys. 2017;5(1):1-6. doi: 10.11648/j.ejb.20170501.11

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  • @article{10.11648/j.ejb.20170501.11,
      author = {Noritaka Yamamoto},
      title = {Tensile Strength of Single Collagen Fibrils Isolated from Tendons},
      journal = {European Journal of Biophysics},
      volume = {5},
      number = {1},
      pages = {1-6},
      doi = {10.11648/j.ejb.20170501.11},
      url = {https://doi.org/10.11648/j.ejb.20170501.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20170501.11},
      abstract = {Tensile failure properties of single collagen fibrils were determined using our original tensile test method. Fibrils were directly isolated from the fascicles of mouse tail tendons. Both the ends of each fibril were wound onto the tips of two microneedles several times using micromanipulators. The fibril and tips were immersed in physiological saline solution. Then, the fibril was stretched to failure by moving the one microneedle. During tensile testing, the fibril was firmly attached to the tips of the microneedles, and broken between the tips with no slippage observed. The diameter of tested 10 fibrils was 410±60 nm (Mean±S.D.). The stress-strain curves of these fibrils were almost linear. Their tensile strength and failure strain were 100±32 MPa and 34±11%, respectively. These values were approximately 480 and 190% of those of the fascicles with the diameter of 81±12 m, respectively.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Tensile Strength of Single Collagen Fibrils Isolated from Tendons
    AU  - Noritaka Yamamoto
    Y1  - 2017/02/16
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ejb.20170501.11
    DO  - 10.11648/j.ejb.20170501.11
    T2  - European Journal of Biophysics
    JF  - European Journal of Biophysics
    JO  - European Journal of Biophysics
    SP  - 1
    EP  - 6
    PB  - Science Publishing Group
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    UR  - https://doi.org/10.11648/j.ejb.20170501.11
    AB  - Tensile failure properties of single collagen fibrils were determined using our original tensile test method. Fibrils were directly isolated from the fascicles of mouse tail tendons. Both the ends of each fibril were wound onto the tips of two microneedles several times using micromanipulators. The fibril and tips were immersed in physiological saline solution. Then, the fibril was stretched to failure by moving the one microneedle. During tensile testing, the fibril was firmly attached to the tips of the microneedles, and broken between the tips with no slippage observed. The diameter of tested 10 fibrils was 410±60 nm (Mean±S.D.). The stress-strain curves of these fibrils were almost linear. Their tensile strength and failure strain were 100±32 MPa and 34±11%, respectively. These values were approximately 480 and 190% of those of the fascicles with the diameter of 81±12 m, respectively.
    VL  - 5
    IS  - 1
    ER  - 

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Author Information
  • Biomechanics Laboratory, Department of Mechanical Engineering, Ritsumeikan University, Shiga, Japan

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