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Synergistic Anticancer Potential of Water Garlic Extract and Copper in a Human Hepatocarcinoma Cell Line

Received: 2 February 2016     Accepted: 18 February 2016     Published: 1 March 2016
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Abstract

Garlic extract in water, supplemented with copper, exerts an enhanced anti-proliferative and a caspase-independent apoptotic activity in a human hepatocarcinoma HepG2 cancer cell line. Furthermore, a DNA double strand break downstream, likely responsible for the observed cell death in HepG2 cell, was evidenced by an early over expression of p53 and p21. This observed synergistic antiproliferative and antitumor capability of water garlic extract, in the presence of copper, might be useful as new tool in cancer prevention and therapy.

Published in Cancer Research Journal (Volume 4, Issue 2)
DOI 10.11648/j.crj.20160402.11
Page(s) 28-31
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Water Garlic Extract, Copper, HepG2, p53, p21, AIF

References
[1] LD. Lawson, BG. Hughes. Characterization of the formation of allicin and other thiosulfinates from garlic. Planta Med. 1992; 58: 345–350.
[2] GR. Fenwick, AB. Hanley. The genus Allium. Part 2. Crit Rev Food Sci Nutr. 1985; 22: 273–377.
[3] GR. Fenwick, AB. Hanley. The genus Allium–Part 1. Crit Rev Food Sci Nutr. 1985; 22: 199–271.
[4] H. Amagase, EM. Schaffer, JA. Milner. Dietary components modify the ability of garlic to suppress 7, 12-dimethylbenz(a)anthracene-induced mammary DNA adducts. J Nutr. 1996; 126: 817–824.
[5] SM. Bird, H. Ge, PC. Uden, et al. High-performance liquid chromatography of seleno amino acids and organo selenium compounds. Speciation by inductively coupled plasma mass spectrometry. J Chromatogr A. 1997; 789: 349–359.
[6] E. Dumont, Y. Ogra, F. Vanhaecke, et al. Liquid chromatography-mass spectrometry (LC-MS): a powerful combination for selenium speciation in garlic (Allium sativum). Anal Bioanal Chem. 2006; 384: 1196–1206.
[7] M Bagul, S Kakumanu, TA Wilson. Crude Garlic Extract Inhibits Cell Proliferation and Induces Cell Cycle Arrest and Apoptosis of Cancer Cells In Vitro. J Med Food. 2015; 18(7): 731-737.
[8] E. De Gianni, C Fimognari. Anticancer Mechanism of Sulfur-Containing Compounds. Enzymes. 2015; 37: 167-192.
[9] R. Uauy, M. Olivares, M. Gonzalez. Essentiality of copper in humans. Am J Clin Nutr. 1998; 67: 952S–959S.
[10] OH. Lowry, NJ. Rosebrough, AL. Farr, et al. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193: 265–275.
[11] E. Hangen, K. Blomgren, P, Bénit, G. Kroemer G, N. Modjtahedi. Life with or without AIF. Trends Biochem. 2010; Sci. 35 (5): 278–287.
[12] LM. Knowles, JA. Milner. Possible mechanism by which allyl sulfides suppress neoplastic cell proliferation. J Nutr. 2001; 131(3s): 1061S–1066S.
[13] N. Druesne-Pecollo, P. Latino-Martel. Modulation of histone acetylation by garlic sulfur compounds. Anticancer Agents Med Chem. 2011; (3) 254–259.
[14] J. P. Kelly, DW. Kaufman, K. Kelley, et al. Recent trends in use of herbal and other natural products. Arch Intern Med. 2005; 165: 281–286.
[15] JY. Yan, FM. Tian, WN. Hu, et al. Apoptosis of human gastric cancer cells line SGC 7901 induced by garlic-derived compound S-allylmercaptocysteine (SAMC). Eur Rev Med Pharmacol Sci. 2013; 17(6): 745–751.
[16] EW. Howard, MT. Ling, CW. Chua, et al. Garlic-derived S-allylmercaptocysteine is a novel in vivo antimetastatic agent for androgen-independent prostate cancer. Clin Cancer Res. 2007; 13(6): 1847–1856.
[17] H. Hu, XP. Zhang, YL. Wang, et al. Identification of a novel function of Id-1 in mediating the anticancer responses of SAMC, a water-soluble garlic derivative, in human bladder cancer cells. Mol Med Rep. 2011; 4(1): 9–16.
[18] D. Liang, Y. Qin, W. Zhao, et al. S-allylmercaptocysteine effectively inhibits the proliferation of colorectal cancer cells under in vitro and in vivo conditions. Cancer let. 2011; 310(1): 69–76.
[19] G. Sigounas, J. Hooker, A. Anagnostou, et al. S-allylmercaptocysteine inhibits cell proliferation and reduces the viability of erythroleukemia, breast, and prostate cancer cell lines. Nutr Cancer. 1997; 27(2): 186–191.
[20] K. Vermeulen, DR. Van Bockstaele, ZN. Berneman. The cell cycle: a review of regulation, deregulation and therapeutic targets in cancer. Cell Prolif. 2003; 36(3): 131–149.
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  • APA Style

    Angelo De Martino, Piera Torricelli, Hanan Mahmoud Abu-Zeid, Anna Shevchenko, Alberto Siciliano, et al. (2016). Synergistic Anticancer Potential of Water Garlic Extract and Copper in a Human Hepatocarcinoma Cell Line. Cancer Research Journal, 4(2), 28-31. https://doi.org/10.11648/j.crj.20160402.11

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    ACS Style

    Angelo De Martino; Piera Torricelli; Hanan Mahmoud Abu-Zeid; Anna Shevchenko; Alberto Siciliano, et al. Synergistic Anticancer Potential of Water Garlic Extract and Copper in a Human Hepatocarcinoma Cell Line. Cancer Res. J. 2016, 4(2), 28-31. doi: 10.11648/j.crj.20160402.11

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    AMA Style

    Angelo De Martino, Piera Torricelli, Hanan Mahmoud Abu-Zeid, Anna Shevchenko, Alberto Siciliano, et al. Synergistic Anticancer Potential of Water Garlic Extract and Copper in a Human Hepatocarcinoma Cell Line. Cancer Res J. 2016;4(2):28-31. doi: 10.11648/j.crj.20160402.11

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  • @article{10.11648/j.crj.20160402.11,
      author = {Angelo De Martino and Piera Torricelli and Hanan Mahmoud Abu-Zeid and Anna Shevchenko and Alberto Siciliano and Simone Beninati},
      title = {Synergistic Anticancer Potential of Water Garlic Extract and Copper in a Human Hepatocarcinoma Cell Line},
      journal = {Cancer Research Journal},
      volume = {4},
      number = {2},
      pages = {28-31},
      doi = {10.11648/j.crj.20160402.11},
      url = {https://doi.org/10.11648/j.crj.20160402.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20160402.11},
      abstract = {Garlic extract in water, supplemented with copper, exerts an enhanced anti-proliferative and a caspase-independent apoptotic activity in a human hepatocarcinoma HepG2 cancer cell line. Furthermore, a DNA double strand break downstream, likely responsible for the observed cell death in HepG2 cell, was evidenced by an early over expression of p53 and p21. This observed synergistic antiproliferative and antitumor capability of water garlic extract, in the presence of copper, might be useful as new tool in cancer prevention and therapy.},
     year = {2016}
    }
    

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    T1  - Synergistic Anticancer Potential of Water Garlic Extract and Copper in a Human Hepatocarcinoma Cell Line
    AU  - Angelo De Martino
    AU  - Piera Torricelli
    AU  - Hanan Mahmoud Abu-Zeid
    AU  - Anna Shevchenko
    AU  - Alberto Siciliano
    AU  - Simone Beninati
    Y1  - 2016/03/01
    PY  - 2016
    N1  - https://doi.org/10.11648/j.crj.20160402.11
    DO  - 10.11648/j.crj.20160402.11
    T2  - Cancer Research Journal
    JF  - Cancer Research Journal
    JO  - Cancer Research Journal
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    PB  - Science Publishing Group
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    UR  - https://doi.org/10.11648/j.crj.20160402.11
    AB  - Garlic extract in water, supplemented with copper, exerts an enhanced anti-proliferative and a caspase-independent apoptotic activity in a human hepatocarcinoma HepG2 cancer cell line. Furthermore, a DNA double strand break downstream, likely responsible for the observed cell death in HepG2 cell, was evidenced by an early over expression of p53 and p21. This observed synergistic antiproliferative and antitumor capability of water garlic extract, in the presence of copper, might be useful as new tool in cancer prevention and therapy.
    VL  - 4
    IS  - 2
    ER  - 

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Author Information
  • Department of Biology, University of Rome “Tor Vergata”, Rome, Italy

  • Department SPES, University of Molise, Campobasso, Italy

  • Faculty of Science, Botany Department, Alexandria University, Alexandria, Egypt

  • People’s Friendship University of Russia, Moscow, Russia

  • Department of Biology, University of Rome “Tor Vergata”, Rome, Italy

  • Department of Biology, University of Rome “Tor Vergata”, Rome, Italy

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