The study was carried out to investigate the possible biodegradation of crude oil as a carbon by the bacterium Pseudomonas aeruginosa isolated from marine environment (Ras El-Menkar- Benghazi- Libya) using basal yeast extract protease peptone-3 (BYP) enriched medium. The isolated bacterium was identified and characterized according to its cultural condition and microbial biochemical properties. Different experiments were developed throughout this study to stimulate bacterial growth and production of single cell protein (SCP). The results show that the optimal concentration of crude oil as a carbon source for the highest bacterial growth (1.14g/l), and production of SCP (0.65g/l; 57.02% of the biomass dry weight) was 1%. This was required to utilize up to 50.6% of oil as a carbon source. As to the nitrogen source, the optimal concentration of ammonium chloride was 0.1%, in which the bacterial growth and SCP production increased to 1.23 g/l and 0.67 g/l respectively. The stimulating effects of organic and inorganic factors on the bacterial growth and SCP production was also tested. Addition of inorganic nutrients such as potassium phosphate (0.05%), magnesium sulphate (0.01%), and organic nutrient in the form of yeast extract (0.1%) to the fermentation medium slightly promoted the bacterial growth which reflected positively on SCP production and the percentage of the consumed crude oil, (>57%) at final pH value of 8.0. The obtained results indicated that the isolated Pseudomonas aeruginosa posses the ability to utilize the crude oil and use it as a carbon for bacterial growth and production of SCP.
Published in | Biochemistry and Molecular Biology (Volume 9, Issue 2) |
DOI | 10.11648/j.bmb.20240902.12 |
Page(s) | 37-47 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Crude Oil, Pseudomonas aeruginosa, SCP, Nitrogen Source, Inorganic and Organic Factors
Tests | Results |
---|---|
Catalase | +ve |
Oxidase | +ve |
Urease | +ve |
Citrate | +ve |
Indol | +ve |
Motality | +ve |
Methyl red | -ve |
Gelatin decomposition | +ve |
Growth on MacConky | +ve |
Growth on Citramide | +ve |
Characters | results |
---|---|
Colonies shape | Circular |
Colonies edge | Irregular |
Cells shape | Strenuous |
Colonies color | Creamy |
Colonies high | High |
Gram staining | Negative |
Stain production | Green stain |
crude oil% | consumed crude oil% | SCP g/l | Biomass dry weight g/l | SCP% | final PH |
---|---|---|---|---|---|
0.25 | 35.2 | 0.36 ± 0.045 | 0.72 ± 0.004 | 49.44 | 8 |
0.5 | 49.4 | 0.55 ± 0.009 | 1.10 ± 0.003 | 50.09 | 8.24 |
1 | 50.6 | 0.65 ± 0.036 | 1.14 ± 0.015 | 57.02 | 8.18 |
1.25 | 42.4 | 0.57 ± 0.071 | 1.00 ± 0.053 | 56.9 | 8.14 |
1.5 | 24.82 | 0.32 ± 0.052 | 0.80 ± 0.073 | 40 | 8.32 |
Ammonium chloride gm/100 ml | consumed crude oil% | SCP g/l | Biomass dry weight g/l | SCP% | final pH |
---|---|---|---|---|---|
0.06 | 15.2 | 0.26 ± 0.007 | 0.66 ± 0.041 | 34.54 | 8.12 |
0.08 | 37.2 | 0.35 ± 0.025 | 0.82 ± 0.025 | 43.75 | 8.12 |
0.1 | 58.35 | 0.67 ± 0.006 | 1.23 ± 0.37 | 54.95 | 8.24 |
0.12 | 40.64 | 0.53 ± 0.004 | 1.04 ± 0.001 | 51.01 | 8.1 |
0.14 | 28.41 | 0.36 ± 0.072 | 0.75 ± 0.006 | 48.31 | 8.08 |
0.16 | 18.53 | 0.24 ± 0.008 | 0.62 ± 0.006 | 38.87 | 8.13 |
Magnesium sulphate gm/100 ml | consumed crude oil % | SCP g/l | Biomass dry weight g/l | SCP % | final PH |
---|---|---|---|---|---|
0.006 | 25.33 | 0.30 ± 0.046 | 0.77 ± 0.008 | 39.14 | 7.77 |
0.008 | 40.1 | 0.42 ± 0.006 | 0.91 ± 0.008 | 45.83 | 7.73 |
0.01 | 53.01 | 0.71 ± 0.051 | 1.27 ± 0.052 | 55.9 | 8.11 |
0.012 | 38.22 | 0.39 ± 0.008 | 0.88 ± 0.053 | 44.56 | 7.89 |
0.014 | 15.6 | 0.23 ± 0.038 | 0.65 ± 0.009 | 35.87 | 7.91 |
Yeast Extract | consumed crude oil% | SCP g/l | Biomass dry weight g/l | SCP% | final PH |
---|---|---|---|---|---|
0.06 | 46.55 | 0.42 ± 0.038 | 0.86 ± 0.036 | 48.7 | 7.87 |
0.08 | 48.41 | 0.57 ± 0.042 | 0.98 ± 0.009 | 53.78 | 7.82 |
0.1 | 57.36 | 0.74 ± 0.051 | 1.29 ± 0.053 | 57.01 | 8.24 |
0.12 | 41.94 | 0.43 ± 0.059 | 0.94 ± 0.062 | 45.38 | 7.9 |
0.14 | 19.22 | 0.21 ± 0.63 | 0.55 ± 0.066 | 38.09 | 7.78 |
Potassium phosphate % | consumed crude oil% | SCP g/l | Biomass dry weight g/l | SCP% | final pH |
---|---|---|---|---|---|
0.01 | 33.64 | 0.40 ± 0.56 | 0.90 ± 0.063 | 44.21 | 7.43 |
0.03 | 50.52 | 0.55 ± 0.071 | 1.08 ± 0.007 | 51.04 | 7.67 |
0.05 | 57.75 | 0.67 ± 0.045 | 1.24 ± 0.018 | 54.03 | 8.0 |
0.07 | 36.66 | 0.48 ± 0.072 | 0.82 ± 0.015 | 58.22 | 7.32 |
0.09 | 9.22 | 0.36 ± 0.062 | 0.64 ± 0.053 | 56.54 | 7.21 |
BYP | Basal Yeast Extract Protease Peptone-3 |
SCP | Single Cell Protein |
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APA Style
Haider, M. M., Rages, A. A., Al-Mergany, H. (2024). Conditional Optimization of Single Cell Protein Production from Crude Oil by Pseudomonas aeruginosa. Biochemistry and Molecular Biology, 9(2), 37-47. https://doi.org/10.11648/j.bmb.20240902.12
ACS Style
Haider, M. M.; Rages, A. A.; Al-Mergany, H. Conditional Optimization of Single Cell Protein Production from Crude Oil by Pseudomonas aeruginosa. Biochem. Mol. Biol. 2024, 9(2), 37-47. doi: 10.11648/j.bmb.20240902.12
@article{10.11648/j.bmb.20240902.12, author = {Mustafa Mohammad Haider and Ashraf Anwar Rages and Huda Al-Mergany}, title = {Conditional Optimization of Single Cell Protein Production from Crude Oil by Pseudomonas aeruginosa }, journal = {Biochemistry and Molecular Biology}, volume = {9}, number = {2}, pages = {37-47}, doi = {10.11648/j.bmb.20240902.12}, url = {https://doi.org/10.11648/j.bmb.20240902.12}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20240902.12}, abstract = {The study was carried out to investigate the possible biodegradation of crude oil as a carbon by the bacterium Pseudomonas aeruginosa isolated from marine environment (Ras El-Menkar- Benghazi- Libya) using basal yeast extract protease peptone-3 (BYP) enriched medium. The isolated bacterium was identified and characterized according to its cultural condition and microbial biochemical properties. Different experiments were developed throughout this study to stimulate bacterial growth and production of single cell protein (SCP). The results show that the optimal concentration of crude oil as a carbon source for the highest bacterial growth (1.14g/l), and production of SCP (0.65g/l; 57.02% of the biomass dry weight) was 1%. This was required to utilize up to 50.6% of oil as a carbon source. As to the nitrogen source, the optimal concentration of ammonium chloride was 0.1%, in which the bacterial growth and SCP production increased to 1.23 g/l and 0.67 g/l respectively. The stimulating effects of organic and inorganic factors on the bacterial growth and SCP production was also tested. Addition of inorganic nutrients such as potassium phosphate (0.05%), magnesium sulphate (0.01%), and organic nutrient in the form of yeast extract (0.1%) to the fermentation medium slightly promoted the bacterial growth which reflected positively on SCP production and the percentage of the consumed crude oil, (>57%) at final pH value of 8.0. The obtained results indicated that the isolated Pseudomonas aeruginosa posses the ability to utilize the crude oil and use it as a carbon for bacterial growth and production of SCP. }, year = {2024} }
TY - JOUR T1 - Conditional Optimization of Single Cell Protein Production from Crude Oil by Pseudomonas aeruginosa AU - Mustafa Mohammad Haider AU - Ashraf Anwar Rages AU - Huda Al-Mergany Y1 - 2024/08/15 PY - 2024 N1 - https://doi.org/10.11648/j.bmb.20240902.12 DO - 10.11648/j.bmb.20240902.12 T2 - Biochemistry and Molecular Biology JF - Biochemistry and Molecular Biology JO - Biochemistry and Molecular Biology SP - 37 EP - 47 PB - Science Publishing Group SN - 2575-5048 UR - https://doi.org/10.11648/j.bmb.20240902.12 AB - The study was carried out to investigate the possible biodegradation of crude oil as a carbon by the bacterium Pseudomonas aeruginosa isolated from marine environment (Ras El-Menkar- Benghazi- Libya) using basal yeast extract protease peptone-3 (BYP) enriched medium. The isolated bacterium was identified and characterized according to its cultural condition and microbial biochemical properties. Different experiments were developed throughout this study to stimulate bacterial growth and production of single cell protein (SCP). The results show that the optimal concentration of crude oil as a carbon source for the highest bacterial growth (1.14g/l), and production of SCP (0.65g/l; 57.02% of the biomass dry weight) was 1%. This was required to utilize up to 50.6% of oil as a carbon source. As to the nitrogen source, the optimal concentration of ammonium chloride was 0.1%, in which the bacterial growth and SCP production increased to 1.23 g/l and 0.67 g/l respectively. The stimulating effects of organic and inorganic factors on the bacterial growth and SCP production was also tested. Addition of inorganic nutrients such as potassium phosphate (0.05%), magnesium sulphate (0.01%), and organic nutrient in the form of yeast extract (0.1%) to the fermentation medium slightly promoted the bacterial growth which reflected positively on SCP production and the percentage of the consumed crude oil, (>57%) at final pH value of 8.0. The obtained results indicated that the isolated Pseudomonas aeruginosa posses the ability to utilize the crude oil and use it as a carbon for bacterial growth and production of SCP. VL - 9 IS - 2 ER -