Research Article
Evaluation of Mutagenic Potential of Nitrosamines Using the Enhanced Ames Test with Hamster Liver S9 Activation
Samit Kadam*
,
Aditya Dipakrao Hajare
Issue:
Volume 11, Issue 3, September 2026
Pages:
42-58
Received:
17 July 2026
Accepted:
30 July 2026
Published:
20 August 2026
Abstract: Nitrosamines (NAs) and Nitrosamine Drug Substance-Related Impurities (NDSRIs) have recently drawn significant attention because of their strong links to cancer and genetic damage. The standard Ames test, described in Organisation for Economic Co-operation and Development (OECD) Guideline 471, is widely used to assess mutagenicity, but it often struggles with certain nitrosamines, especially those requiring complex metabolic activation or producing weak signals at low doses. To address these limitations, regulators now recommend the Enhanced Ames Test (EAT) for improved sensitivity. In this study, we assessed three nitrosamines, N-nitrosodimethylamine (NDMA), 1?cyclopentyl?4?nitrosopiperazine (CPNP), and N-nitrosodiethylamine (NDEA), using the EAT. We began with cytotoxicity evaluations in Salmonella typhimurium (S. typhi.) strains TA100 and TA1535 to determine the highest non-toxic doses. Each compound was then preincubated with 30% liver S9 enzymes from rats and hamsters for 30 minutes to better replicate metabolic activation. Hamster S9 produced stronger mutagenic responses than rat S9. Among the tested strains, TA1535 showed the highest sensitivity. NDMA produced 2.3-2.7?fold higher mutagenicity in TA1535 compared with other strains. CPNP showed an even stronger effect, with a five?fold increase. NDEA was the most potent, generating nearly a five?fold stronger response with hamster S9 than with rat S9 (88.7?fold vs. 17.2?fold per dose). Overall, these findings highlight that the EAT, combined with optimized metabolic activation, is a robust approach for detecting mutagenic nitrosamines that may otherwise remain undetected.
Abstract: Nitrosamines (NAs) and Nitrosamine Drug Substance-Related Impurities (NDSRIs) have recently drawn significant attention because of their strong links to cancer and genetic damage. The standard Ames test, described in Organisation for Economic Co-operation and Development (OECD) Guideline 471, is widely used to assess mutagenicity, but it often stru...
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Research Article
Serum Biochemical Profiles of Apparently Healthy Goats in Sokoto Metropolis, Northwestern Nigeria: Preliminary Local Reference Values
Issue:
Volume 11, Issue 3, September 2026
Pages:
59-64
Received:
28 August 2026
Accepted:
20 September 2026
Published:
29 October 2026
Abstract: Serum biochemical parameters provide valuable information on the physiological, metabolic, and health status of animals and are commonly used in veterinary clinical practice. However, biochemical values vary with breed, nutrition, management, environmental conditions, age, sex, and physiological status, highlighting the need for locally relevant data. This study examined serum biochemical parameters of goats in Sokoto Metropolis, to produce preliminary local biochemical values for clinical assessment. Forty (40) apparently healthy goats were randomly selected from households, farms, and semi-intensive production systems. Approximately 5 mL of blood was collected from the jugular vein into plain tubes, and serum was analyzed using an automated chemistry analyzer at the Clinical Pathology Laboratory. Total protein, albumin, globulin, ALT, AST, ALP, urea, creatinine, total bilirubin, and direct bilirubin were Parameters assessed. The data were summarized using descriptive statistics. Mean ± SD values were 8.28 ± 1.02 g/dL for total protein, 2.61 ± 0.23 g/dL for albumin, 5.67 ± 1.05 g/dL for globulin, 40.89 ± 71.10 U/L for ALT, 32.23 ± 36.84 U/L for AST, 99.49 ± 78.65 U/L for ALP, 47.64 ± 24.87 mg/dL for urea, 4.35 ± 0.53 mg/dL for creatinine, 3.84 ± 4.02 mg/dL for total bilirubin, and 6.52 ± 1.56 mg/dL for direct bilirubin. Most values were mostly comparable with previous reports for indigenous Nigerian goats, even though considerable variation occurred in some liver enzymes and bilirubin indices. These findings provide preliminary local biochemical data and emphasize the importance of regional, nutritional, environmental, and management factors in interpreting serum biochemical results.
Abstract: Serum biochemical parameters provide valuable information on the physiological, metabolic, and health status of animals and are commonly used in veterinary clinical practice. However, biochemical values vary with breed, nutrition, management, environmental conditions, age, sex, and physiological status, highlighting the need for locally relevant da...
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