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NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon

Received: 27 October 2023     Accepted: 14 November 2023     Published: 30 November 2023
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Abstract

Background: Diabetes is increasingly prevalent in malaria endemic settings like Cameroon thus contributing to a double burden in the management of these inflammatory diseases. Studies have shown that NLRP3 inflammasome plays a key role in type-2 diabetes (T2DM) and malaria induced inflammation. However, the hypothesis that the Single Nucleotides Polymorphisms (rs10754558 and rs4612666) in the NLRP3 gene could be associated with T2DM and malaria comorbidity is relatively new. This study aimed at determining the association between NLRP3 rs10754558 and rs4612666 Single Nucleotide Polymorphisms with susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in Yaoundé, Cameroon. Methods: A case-control study was performed on 100 conveniently collected blood samples, spotted on Whartman N° 3 filter paper from which DNA was extracted by the chelex-100 boiling method. Nested-PCR was used to confirm the presence of malaria and speciate Plasmodium spp. Genotyping of the NLRP3 gene SNPs was performed using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The Chi-square test (X2) was used to establish associations. A P-value of <0.05 was considered significant. Results: The mean age of the study population was 55±12.38 years. Eighty-eight (88) participants were diagnosed with T2DM, whereof 7 (7.95%) were ascertained by nested-PCR to harbour malaria; P. falciparum being the dominant circulating species. The most predominant genotype and allele for rs10754558 and rs4612666, was the heterozygous genotype GC and wildtype allele G (52.00%, 69.00%), and the homozygous mutant genotype CC and mutant allele C (63.00%, 76.50%) respectively. No statistical significance was found between the comorbid group and diabetes positive /malaria negative (D+M-) control group for the rs10754558 and rs4612666 SNPs. Statistical significance was found between the comorbid group and the diabetes negative/ malaria positive (D-M+) control group for the rs4612666 SNP. Individuals possessing the CC genotypes were 8 times more susceptible to diabetes and malaria comorbidity (OR=8.000, P=0.043), whereas individuals possessing the TC genotype were less susceptible (OR=0.079, P=0.030). Conclusion: An association was found between the NLRP3 rs4612666 SNP and susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in our study.

Published in Biochemistry and Molecular Biology (Volume 8, Issue 3)
DOI 10.11648/j.bmb.20230803.11
Page(s) 37-44
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

NLRP3, Gene Polymorphism, Type 2 Diabetes Mellitus, Malaria, Co-morbidity, Susceptibility

References
[1] R. M. Carrillo-larco, C. Altez-fernandez, and C. Ugarte-gil, “Is diabetes associated with malaria and malaria severity? A systematic review of observational studies [version 3; peer review: 1 approved, 1 approved with reservations],” Wellcome Open Res., vol. 4, no. 136, pp. 1–19, 2019, doi: https://doi.org/10.12688/wellcomeopenres.15467.3.
[2] World Health Organization, “Classification of Diabetes Mellitus”. 2019 [Online]. Available: https://www.who.int/publications-detail-redirect/classification-of-diabetes-mellitus. [Accessed 21 April 2019].
[3] World Health Organization, “Global Status Report on noncommunicable diseases,” 2014 [Online] Available: https://reliefweb.int/report/world/global-status-report-noncommunicable-diseases-2014-attaining-nine-global. [Accessed 15 June 2020].
[4] International Diabetes Federation, D. Atlas, “Idf diabetes atlas”. 2019 [Online]. Available: https://diabetesatlas.org/atlas/ninth-edition/. [Accessed 02 March 2020].
[5] World Health Organization, “Global Report On Diabetes,” 2016 [Online] Available: https://www.who.int/publications/i/item/9789241565257 [Accessed 16 July 2020].
[6] A. Nkondjio et al., “Review of malaria situation in Cameroon: technical viewpoint on challenges and prospects for disease elimination,” Parasit. Vectors, pp. 1–23, 2019, doi: 10.1186/s13071-019-3753-8.
[7] M. A. Phillips, J. N. Burrows, C. Manyando, R. H. Van Huijsduijnen, W. C. Van Voorhis, and T. N. C. Wells, “Malaria,” Nature, vol. 3, pp. 1–24, 2017, doi: 10.1038/nrdp.2017.50.
[8] B. Singh and C. Daneshvar, “Human Infections and Detection of Plasmodium knowlesi,” Clin. Microbiol. Rev., vol. 26, no. 2, pp. 165–184, 2013, doi: 10.1128/CMR.00079-12.
[9] World Health Organization, “20 Years of Global Progress and Challenges”, vol. WHO/HTM/GM, no. December. 2020 [Online]. Available: [Accessed 30 November 2020].
[10] I. Danquah, G. Bedu-addo, and F. P. Mockenhaupt, “Type 2 Diabetes Mellitus and Increased Risk for Malaria Infection,” Emerg. Infect. Dis., vol. 16, no. 10, 2010, doi: DOI: 10.3201/eid1610.100399.
[11] I. C. Eze et al., “Asymptomatic Plasmodium infection and glycemic control in adults: Results from a population-based survey in south-central Côte d’Ivoire,” Diabetes Res. Clin. Pract., vol. 156, Oct. 2019, doi: 10.1016/j.diabres.2019.107845.
[12] B. Udoh, B. Iwalokun, E. Etukumana, and J. Amoo, “Asymptomatic falciparum malaria and its effects on type 2 diabetes mellitus patients in Lagos, Nigeria,” Saudi J. Med. Med. Sci., vol. 8, no. 1, p. 32, 2020, doi: 10.4103/sjmms.sjmms_178_18.
[13] L. Franchi, R. Muñoz-planillo, and G. Núñez, “review Sensing and reacting to microbes through the inflammasomes,” Nat. Immunol., vol. 13, no. 4, 2012, doi: 10.1038/ni.2231.
[14] M. Lamkanfi and V. M. Dixit, “The Inflammasomes,” PLoS Pathog., vol. 5, no. 12, pp. 1–5, 2009, doi: 10.1371/journal.ppat.1000510.
[15] R. T. Gazzinelli, P. Kalantari, and K. A. Fitzgerald, “Innate sensing of malaria parasites,” Nat. Publ. Gr., pp. 1–14, 2014, doi: 10.1038/nri3742.
[16] P. Kalantari et al., “Dual engagement of the NLRP3 and AIM2 inflammasomes by plasmodium-derived hemozoin and DNA during Malaria,” Cell Rep., vol. 6, no. 1, pp. 196–210, 2014, doi: 10.1016/j.celrep.2013.12.014.
[17] E. Mendenhall, “Syndemics: a new path for global health research,” The Lancet, vol. 389, no. 10072. Lancet Publishing Group, pp. 889–891, Mar. 2017. doi: 10.1016/S0140-6736(17)30602-5.
[18] R. van Crevel, S. van de Vijver, and D. A. J. Moore, “The global diabetes epidemic: what does it mean for infectious diseases in tropical countries?,” The Lancet Diabetes and Endocrinology, vol. 5, no. 6. Lancet Publishing Group, pp. 457–468, Jun. 2017. doi: 10.1016/S2213-8587(16)30081-X.
[19] J. M. Fernández-Real and J. C. Pickup, “Innate immunity, insulin resistance and type 2 diabetes,” Diabetologia, vol. 55, no. 2, pp. 273–278, Feb. 2012, doi: 10.1007/s00125-011-2387-y.
[20] J. Chmelar, K. Chung, and T. Chavakis, “The role of innate immune cells in obese adipose tissue inflammation and development of insulin resistance Introduction:,” Thromb. Haemost., vol. 109, pp. 399–406, 2013, doi: 10.1160/TH12-09-0703.
[21] Y. Zheng, D. Zhang, L. Zhang, M. Fu, Y. Zeng, and R. Russell, “Variants of NLRP3 gene are associated with insulin resistance in Chinese Han population with type-2 diabetes,” Gene, vol. 530, no. 1, pp. 151–154, 2013, doi: 10.1016/j.gene.2013.07.082.
[22] D. Zhou et al., “The NLRP3 rs10754558 Polymorphism Is Associated with the Occurrence and Prognosis of Coronary Artery Disease in the Chinese Han Population,” Biomed Res. Int., vol. 2016, 2016, doi: http://dx.doi.org/10.1155/2016/3185397.
[23] L. Bai et al., “Association of two common SNPs in NLRP3 with risk of type 2 diabetes mellitus and their interaction with environmental factors,” Int J Clin Exp Pathol, vol. 9, no. 10, pp. 10499–10506, 2016.
[24] J. A. Critchley et al., “Defining a Research Agenda to Address the Converging Epidemics of Tuberculosis and Diabetes: Part 1: Epidemiology and Clinical Management,” Chest, vol. 152, no. 1. Elsevier Inc, pp. 165–173, Jul. 2017. doi: 10.1016/j.chest.2017.04.155.
[25] R. Bissaya, R. T. Ghogomu, A. Moundi, B. Njom, and S. Kanouo, “Utilisation des données géologiques et gestion des informations multi-sources pour l ’ analyse de l ’ aléa glissement de terrain / éboulement dans le secteur Nord-Ouest de la région de Yaoundé Résumé,” Afrique Sci., vol. 10, no. 3, pp. 113–133, 2014.
[26] C. V. Plowe, A. Djimde, M. Bouare, O. Doumbo, and T. E. Wellems, “Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase: Polymerase chain reaction methods for surveillance in Africa,” American Journal of Tropical Medicine and Hygiene, vol. 52, no. 6. pp. 565–568, 1995. doi: 10.4269/ajtmh.1995.52.565.
[27] P. S. Walsh, D. A. Metzger, and R. Higuchi, “Biotechniques 30th anniversary gem Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material,” Biotechniques, vol. 54, no. 3, pp. 506–513, 2013.
[28] S. Georges, S. Viriyakbosola, X. Ping, and W. Jarra, “High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction,” Mol. Biochem. Parasitol., vol. 61, pp. 315–320, 1993.
[29] G. B. Grezzana, J. L. Da Costa Vieira, and V. L. Portal, “Single-nucleotide polymorphisms: A perspective of cardiovascular prevention,” Revista da Associacao Medica Brasileira, vol. 61, no. 5. Associacao Medica Brasileira, pp. 458–468, Sep. 2015. doi: 10.1590/1806-9282.61.05.458.
[30] W. M. Howell and M. J. Rose-zerilli, “Cytokine Gene Polymorphisms, Cancer Susceptibility, and Prognosis 1 – 3,” J. Nutr., pp. 8–13, 2007.
[31] A. Pontillo, L. Brandao, R. Guimaraes, L. Segat, J. Araujo, and S. Crovella, “Two SNPs in NLRP3 gene are involved in the predisposition to type-1 diabetes and celiac disease in a pediatric population from northeast,” Autoimmunity, vol. 43, no. 8, pp. 583–589, 2010, doi: 10.3109/08916930903540432.
[32] Q. B. Zhang, Y. F. Qing, Y. L. He, W. G. Xie, and J. G. Zhou, “Association of NLRP3 polymorphisms with susceptibility to primary gouty arthritis in a Chinese han population,” Clin. Rheumatol., vol. 37, no. 1, pp. 235–244, 2018, doi: 10.1007/s10067-017-3900-6.
[33] O. O. Oguntibeju, “Type 2 diabetes mellitus, oxidative stress and inflammation: examining the links,” Int J Physiol Pathophysiol Pharmacol, vol. 11, no. 3, pp. 45–63, 2019.
[34] L. Cheng, R. Yin, S. Yang, X. Pan, and A. Ma, “Rs4612666 Polymorphism of the NLRP3 Gene Is Associated with the Occurrence of Large Artery Atherosclerotic Ischemic Strokes and Microembolic Signals,” Biomed Res. Int., vol. 2018, 2018, doi: https://doi.org/10.1155/2018/6345805.
[35] H. Yaribeygi, N. Katsiki, A. E. Butler, and A. Sahebkar, “Effects of antidiabetic drugs on NLRP3 inflammasome activity, with a focus on diabetic kidneys,” Drug Discov. Today, vol. 24, no. 1, pp. 256–262, 2019, doi: 10.1016/j.drudis.2018.08.005.
[36] J. J. E. Koopman, D. Van Bodegom, J. W. Jukema, and R. G. J. Westendorp, “Risk of Cardiovascular Disease in a Traditional African Population with a High Infectious Load: A Population- Based Study,” PLoS One, vol. 7, no. 10, 2012, doi: 10.1371/journal.pone.0046855.
[37] Y. He, H. Hara, and G. Núñez, “Mechanism and Regulation of NLRP3 In fl ammasome Activation,” Cellpress, vol. xx, pp. 1–10, 2016, doi: 10.1016/j.tibs.2016.09.002.
[38] K. E. Lyke et al., “Serum Levels of the Proinflammatory Cytokines Interleukin-1 Beta (IL-1 ␤), IL-6, IL-8, IL-10, Tumor Necrosis Factor Alpha, and IL-12 (p70) in Malian Children with Severe Plasmodium falciparum Malaria and Matched Uncomplicated Malaria or Healthy Co,” Infect. Immun., vol. 72, no. 10, pp. 5630–5637, 2004, doi: 10.1128/IAI.72.10.5630.
[39] M. T. Shio et al., “Malarial Hemozoin Activates the NLRP3 Inflammasome through Lyn and Syk Kinases,” vol. 5, no. 8, 2009, doi: 10.1371/journal.ppat.1000559.
[40] S. Kesavardhana and T. Kanneganti, “Mechanisms governing inflammasome activation, assembly and pyroptosis induction,” Int. Immunol., vol. 29, pp. 201–210, 2017, doi: https://doi.org/10.1093/intimm%2Fdxx018.
[41] M. S. J. Mangan, E. J. Olhava, W. R. Roush, and H. M. Seidel, “Targeting the NLRP3 inflammasome in inflammatory diseases,” Nature, vol. 17, pp. 588–606, 2018, doi: 10.1038/nrd.2018.97.
[42] E. Tourkochristou, I. Aggeletopoulou, C. Konstantakis, and C. Triantos, “Role of NLRP3 inflammasome in inflammatory bowel diseases Author contributions:,” World J Gastroenterol, vol. 25, no. 33, pp. 4796–4804, 2019, doi: 10.3748/wjg.v25.i33.4796.
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  • APA Style

    Fonyuy, M. V., Tah, C. F., Nji, A. M., Winnie, I. M. K., Chedjou, J. P. K., et al. (2023). NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon. Biochemistry and Molecular Biology, 8(3), 37-44. https://doi.org/10.11648/j.bmb.20230803.11

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    Fonyuy, M. V.; Tah, C. F.; Nji, A. M.; Winnie, I. M. K.; Chedjou, J. P. K., et al. NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon. Biochem. Mol. Biol. 2023, 8(3), 37-44. doi: 10.11648/j.bmb.20230803.11

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    Fonyuy MV, Tah CF, Nji AM, Winnie IMK, Chedjou JPK, et al. NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon. Biochem Mol Biol. 2023;8(3):37-44. doi: 10.11648/j.bmb.20230803.11

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  • @article{10.11648/j.bmb.20230803.11,
      author = {Marie-Claire Vernyuy Fonyuy and Calvino Fomboh Tah and Akindeh Mbuh Nji and Isabelle Mboutchuin Kamdem Winnie and Jean Paul Kengne Chedjou and Magellan Guewo Fokeng and Aristid Herve Ekollo Mbange and Wilfried Olivier Ngandjeu Tchamdjeu and Lesley Ngum Ngum and Peter Thelma Ngwa Niba and Rodrigue Essomba Foe and Cedric Hermann Dongmo and Carine Nguefeu Nkenfou-Tchinda and Rhoda Bongshe Laban and Yusinyu Eugenie Mumah and Eugene Sobngwi and Jean Claude Mbanya and Wilfred Fon Mbacham},
      title = {NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon},
      journal = {Biochemistry and Molecular Biology},
      volume = {8},
      number = {3},
      pages = {37-44},
      doi = {10.11648/j.bmb.20230803.11},
      url = {https://doi.org/10.11648/j.bmb.20230803.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20230803.11},
      abstract = {Background: Diabetes is increasingly prevalent in malaria endemic settings like Cameroon thus contributing to a double burden in the management of these inflammatory diseases. Studies have shown that NLRP3 inflammasome plays a key role in type-2 diabetes (T2DM) and malaria induced inflammation. However, the hypothesis that the Single Nucleotides Polymorphisms (rs10754558 and rs4612666) in the NLRP3 gene could be associated with T2DM and malaria comorbidity is relatively new. This study aimed at determining the association between NLRP3 rs10754558 and rs4612666 Single Nucleotide Polymorphisms with susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in Yaoundé, Cameroon. Methods: A case-control study was performed on 100 conveniently collected blood samples, spotted on Whartman N° 3 filter paper from which DNA was extracted by the chelex-100 boiling method. Nested-PCR was used to confirm the presence of malaria and speciate Plasmodium spp. Genotyping of the NLRP3 gene SNPs was performed using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The Chi-square test (X2) was used to establish associations. A P-value of Results: The mean age of the study population was 55±12.38 years. Eighty-eight (88) participants were diagnosed with T2DM, whereof 7 (7.95%) were ascertained by nested-PCR to harbour malaria; P. falciparum being the dominant circulating species. The most predominant genotype and allele for rs10754558 and rs4612666, was the heterozygous genotype GC and wildtype allele G (52.00%, 69.00%), and the homozygous mutant genotype CC and mutant allele C (63.00%, 76.50%) respectively. No statistical significance was found between the comorbid group and diabetes positive /malaria negative (D+M-) control group for the rs10754558 and rs4612666 SNPs. Statistical significance was found between the comorbid group and the diabetes negative/ malaria positive (D-M+) control group for the rs4612666 SNP. Individuals possessing the CC genotypes were 8 times more susceptible to diabetes and malaria comorbidity (OR=8.000, P=0.043), whereas individuals possessing the TC genotype were less susceptible (OR=0.079, P=0.030). Conclusion: An association was found between the NLRP3 rs4612666 SNP and susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in our study.
    },
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon
    AU  - Marie-Claire Vernyuy Fonyuy
    AU  - Calvino Fomboh Tah
    AU  - Akindeh Mbuh Nji
    AU  - Isabelle Mboutchuin Kamdem Winnie
    AU  - Jean Paul Kengne Chedjou
    AU  - Magellan Guewo Fokeng
    AU  - Aristid Herve Ekollo Mbange
    AU  - Wilfried Olivier Ngandjeu Tchamdjeu
    AU  - Lesley Ngum Ngum
    AU  - Peter Thelma Ngwa Niba
    AU  - Rodrigue Essomba Foe
    AU  - Cedric Hermann Dongmo
    AU  - Carine Nguefeu Nkenfou-Tchinda
    AU  - Rhoda Bongshe Laban
    AU  - Yusinyu Eugenie Mumah
    AU  - Eugene Sobngwi
    AU  - Jean Claude Mbanya
    AU  - Wilfred Fon Mbacham
    Y1  - 2023/11/30
    PY  - 2023
    N1  - https://doi.org/10.11648/j.bmb.20230803.11
    DO  - 10.11648/j.bmb.20230803.11
    T2  - Biochemistry and Molecular Biology
    JF  - Biochemistry and Molecular Biology
    JO  - Biochemistry and Molecular Biology
    SP  - 37
    EP  - 44
    PB  - Science Publishing Group
    SN  - 2575-5048
    UR  - https://doi.org/10.11648/j.bmb.20230803.11
    AB  - Background: Diabetes is increasingly prevalent in malaria endemic settings like Cameroon thus contributing to a double burden in the management of these inflammatory diseases. Studies have shown that NLRP3 inflammasome plays a key role in type-2 diabetes (T2DM) and malaria induced inflammation. However, the hypothesis that the Single Nucleotides Polymorphisms (rs10754558 and rs4612666) in the NLRP3 gene could be associated with T2DM and malaria comorbidity is relatively new. This study aimed at determining the association between NLRP3 rs10754558 and rs4612666 Single Nucleotide Polymorphisms with susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in Yaoundé, Cameroon. Methods: A case-control study was performed on 100 conveniently collected blood samples, spotted on Whartman N° 3 filter paper from which DNA was extracted by the chelex-100 boiling method. Nested-PCR was used to confirm the presence of malaria and speciate Plasmodium spp. Genotyping of the NLRP3 gene SNPs was performed using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The Chi-square test (X2) was used to establish associations. A P-value of Results: The mean age of the study population was 55±12.38 years. Eighty-eight (88) participants were diagnosed with T2DM, whereof 7 (7.95%) were ascertained by nested-PCR to harbour malaria; P. falciparum being the dominant circulating species. The most predominant genotype and allele for rs10754558 and rs4612666, was the heterozygous genotype GC and wildtype allele G (52.00%, 69.00%), and the homozygous mutant genotype CC and mutant allele C (63.00%, 76.50%) respectively. No statistical significance was found between the comorbid group and diabetes positive /malaria negative (D+M-) control group for the rs10754558 and rs4612666 SNPs. Statistical significance was found between the comorbid group and the diabetes negative/ malaria positive (D-M+) control group for the rs4612666 SNP. Individuals possessing the CC genotypes were 8 times more susceptible to diabetes and malaria comorbidity (OR=8.000, P=0.043), whereas individuals possessing the TC genotype were less susceptible (OR=0.079, P=0.030). Conclusion: An association was found between the NLRP3 rs4612666 SNP and susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in our study.
    
    VL  - 8
    IS  - 3
    ER  - 

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Author Information
  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Strateos, 458 Carlton Court, South San Francisco, California, United States of America

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Fobang Institutes for Innovations in Science and Technology, Simbock, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Fobang Institutes for Innovations in Science and Technology, Simbock, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Physiology and Pharmacology, Faculty of Medicine and Biomedical Science, University of Yaounde I, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Buea, Buea, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Physiology and Pharmacology, Faculty of Medicine and Biomedical Science, University of Yaounde I, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; University Institute of Technologies, University of Ngaoundere, Ngaoundere, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Physiology and Pharmacology, Faculty of Medicine and Biomedical Science, University of Yaounde I, Yaounde, Cameroon; Institute of Medicine and Medicinal Plants Studies, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Physiology and Pharmacology, Faculty of Medicine and Biomedical Science, University of Yaounde I, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

  • Gandhi Institute of Technology and Management (GITAM) University, Visakhapatnam, India

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Department of Internal Medicine, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Internal Medicine, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon

  • Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Fobang Institutes for Innovations in Science and Technology, Simbock, Yaounde, Cameroon; Department of Biochemistry, Physiology and Pharmacology, Faculty of Medicine and Biomedical Science, University of Yaounde I, Yaounde, Cameroon

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