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The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis

Received: 18 September 2018     Accepted: 5 October 2018     Published: 1 November 2018
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Abstract

In a comparative aspect, the functional state of inflammation effector cells in animals infected with various plasmid types of Yersinia pseudotuberculosis was studied. The metabolic activity of peritoneal exudate cells has been investigated in an experimental infection caused by four plasmid types of Y. pseudotuberculosis: type 82+: 48+, containing two plasmids pVM 82 and pYV; type 82+: 48- containing single pVM 82 plasmid; type 48+: 82- containing single pYV plasmid; plasmid-free type 48-: 82-. The parameters of enzyme activity (ATP-ase, 5'-nucleotidase, lactate dehydrogenase, myeloperoxidase) and the level of nitric oxide metabolites were determined. The variability of the metabolic activity of the cells in the inflammatory focus (peritoneal exudate containing neutrophils and macrophages) in infected animals has been established. In response to infection with Y. pseudotuberculosis strain containing two plasmids pYV and pVM82, the production of the nitric oxide metabolites, rather than the active forms of oxygen, had the primary importance in providing the bactericidal potential of phagocytes, compared to animals infected with a strain containing a single pVM82 plasmid. It was concluded that a special biological effect associated with the pVM 82 plasmid available in the Far Eastern strains of the causative agent of epidemic pseudotuberculosis (Far Eastern scarlet-like fever) was involved in the provision of predominantly nitroxide-dependent bactericidal mechanisms of innate immunity cells in this infection.

Published in Biochemistry and Molecular Biology (Volume 3, Issue 3)
DOI 10.11648/j.bmb.20180303.11
Page(s) 49-55
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Metabolic Activity, Innate Immunity Cells, ATPase, Lactate Dehydrogenase, Myelo Peroxidase, Nitric Oxide Metabolites, Stimulation Index (Т), Variability, Plasmid Types of Yersinia Pseudotu Berculosis

References
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[8] Timchenko N. F., Adgamov R. R., Popov A. F., Psareva E. K., Sobyanin K. A., Gintsburg A. L., Ermplaeva S. A. (2016) East Scarlet-like fever caused by a Few related genotypes of Yersinia pseudotuberculosis. Emerging Infest Dis 22 (3): 503-506.
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Cite This Article
  • APA Style

    Larisa Mikhailovna Somova, Irina Nikolaevna Lyapun, Elena Igorevna Drobot, Felix Nikolayevich Shubin, Natalya Gennadyevna Plekhova. (2018). The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis. Biochemistry and Molecular Biology, 3(3), 49-55. https://doi.org/10.11648/j.bmb.20180303.11

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    ACS Style

    Larisa Mikhailovna Somova; Irina Nikolaevna Lyapun; Elena Igorevna Drobot; Felix Nikolayevich Shubin; Natalya Gennadyevna Plekhova. The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis. Biochem. Mol. Biol. 2018, 3(3), 49-55. doi: 10.11648/j.bmb.20180303.11

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    AMA Style

    Larisa Mikhailovna Somova, Irina Nikolaevna Lyapun, Elena Igorevna Drobot, Felix Nikolayevich Shubin, Natalya Gennadyevna Plekhova. The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis. Biochem Mol Biol. 2018;3(3):49-55. doi: 10.11648/j.bmb.20180303.11

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  • @article{10.11648/j.bmb.20180303.11,
      author = {Larisa Mikhailovna Somova and Irina Nikolaevna Lyapun and Elena Igorevna Drobot and Felix Nikolayevich Shubin and Natalya Gennadyevna Plekhova},
      title = {The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis},
      journal = {Biochemistry and Molecular Biology},
      volume = {3},
      number = {3},
      pages = {49-55},
      doi = {10.11648/j.bmb.20180303.11},
      url = {https://doi.org/10.11648/j.bmb.20180303.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20180303.11},
      abstract = {In a comparative aspect, the functional state of inflammation effector cells in animals infected with various plasmid types of Yersinia pseudotuberculosis was studied. The metabolic activity of peritoneal exudate cells has been investigated in an experimental infection caused by four plasmid types of Y. pseudotuberculosis: type 82+: 48+, containing two plasmids pVM 82 and pYV; type 82+: 48- containing single pVM 82 plasmid; type 48+: 82- containing single pYV plasmid; plasmid-free type 48-: 82-. The parameters of enzyme activity (ATP-ase, 5'-nucleotidase, lactate dehydrogenase, myeloperoxidase) and the level of nitric oxide metabolites were determined. The variability of the metabolic activity of the cells in the inflammatory focus (peritoneal exudate containing neutrophils and macrophages) in infected animals has been established. In response to infection with Y. pseudotuberculosis strain containing two plasmids pYV and pVM82, the production of the nitric oxide metabolites, rather than the active forms of oxygen, had the primary importance in providing the bactericidal potential of phagocytes, compared to animals infected with a strain containing a single pVM82 plasmid. It was concluded that a special biological effect associated with the pVM 82 plasmid available in the Far Eastern strains of the causative agent of epidemic pseudotuberculosis (Far Eastern scarlet-like fever) was involved in the provision of predominantly nitroxide-dependent bactericidal mechanisms of innate immunity cells in this infection.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis
    AU  - Larisa Mikhailovna Somova
    AU  - Irina Nikolaevna Lyapun
    AU  - Elena Igorevna Drobot
    AU  - Felix Nikolayevich Shubin
    AU  - Natalya Gennadyevna Plekhova
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    N1  - https://doi.org/10.11648/j.bmb.20180303.11
    DO  - 10.11648/j.bmb.20180303.11
    T2  - Biochemistry and Molecular Biology
    JF  - Biochemistry and Molecular Biology
    JO  - Biochemistry and Molecular Biology
    SP  - 49
    EP  - 55
    PB  - Science Publishing Group
    SN  - 2575-5048
    UR  - https://doi.org/10.11648/j.bmb.20180303.11
    AB  - In a comparative aspect, the functional state of inflammation effector cells in animals infected with various plasmid types of Yersinia pseudotuberculosis was studied. The metabolic activity of peritoneal exudate cells has been investigated in an experimental infection caused by four plasmid types of Y. pseudotuberculosis: type 82+: 48+, containing two plasmids pVM 82 and pYV; type 82+: 48- containing single pVM 82 plasmid; type 48+: 82- containing single pYV plasmid; plasmid-free type 48-: 82-. The parameters of enzyme activity (ATP-ase, 5'-nucleotidase, lactate dehydrogenase, myeloperoxidase) and the level of nitric oxide metabolites were determined. The variability of the metabolic activity of the cells in the inflammatory focus (peritoneal exudate containing neutrophils and macrophages) in infected animals has been established. In response to infection with Y. pseudotuberculosis strain containing two plasmids pYV and pVM82, the production of the nitric oxide metabolites, rather than the active forms of oxygen, had the primary importance in providing the bactericidal potential of phagocytes, compared to animals infected with a strain containing a single pVM82 plasmid. It was concluded that a special biological effect associated with the pVM 82 plasmid available in the Far Eastern strains of the causative agent of epidemic pseudotuberculosis (Far Eastern scarlet-like fever) was involved in the provision of predominantly nitroxide-dependent bactericidal mechanisms of innate immunity cells in this infection.
    VL  - 3
    IS  - 3
    ER  - 

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Author Information
  • Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation

  • Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation

  • Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation

  • Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation

  • Ministry of Health of Russia, Pacific Medical University, Vladivostok, Russian Federation

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