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Prevalence of Bacterial Microbiota in Tilapia Guineensis Harvested from Buguma Brackish Water Environment River State, Nigeria

Received: 26 March 2020     Accepted: 10 April 2020     Published: 17 June 2020
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Abstract

Brackish water fishes usually harbour human pathogenic bacteria particularly the enterics and coliform groups in their mucosal surfaces. Thus, this study was undertaken to investigate the prevalence of skin and gills microbiota of Tilapia guineensis as to understand their microbial assemblages and as well provide useful insights into the broad dynamics of the fish host-microbial relationship and health status. Thirty samples of Tilapia guineensis were collected from Buguma brackish water environment, River State, Nigeria. The skin and the gills of the tilapia were analyzed microbiologically. Specimen were collected by swabbing aseptically over the skin and gills segment of the fish and then processed for total bacterial count, followed by isolation and identification of isolated bacteria. Microbial load analysis revealed that the total viable count of bacteria ranged from 2.4 to 7.6 x 105 cfu/ml for skin and 2.1 to 5.7 x 106 cfu/ml for gills, and it was observed to fall within the recommended microbiological standard safety limit. A total of thirty-seven (37) bacteria, belonging to fifteen different genera in the family of Vibrionaceae, enterobacteriaceae and Morganellaceae were isolated from the fish. The prevalent rate of the bacterial shows that Proteus vulgaris recorded the highest frequency of occurrence of 16.22%, followed by Vibrio parahaemolyticus with 10.81% occurrence and Provindencia rettgeri, Serratia mercescens and Salmonella sp. trailed them with 8.11%. Kluyyera ascorbate, Citrobacter amalonaticus, Proteus mirabilis, Enterobacter aerogenes, plesiomonas shigelloides and Pseudomonas sp. have the least frequency of occurrence with 2.7% respectively for each. Though the bacterial count of Tilapia guineensis harvested from the water and fish sample did not exceed the standard recommended safety limit. There were several potential pathogenic and spoilage bacteria isolated as part of the fish flora. The presence of these bacteria could poses or constitute a public health risk.

Published in American Journal of Bioscience and Bioengineering (Volume 8, Issue 3)
DOI 10.11648/j.bio.20200803.11
Page(s) 36-40
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Bacterial Flora, Tilapia guineensis, Prevalence, Brackish Water, Pathogen

References
[1] Owusi-Frimpong, M., Attipoe, F. U. K. and Padi, J. N. (2005). Comparison of some traits of economic importance in Tilapias with particular reference to their culture in Ghana. NAGA 22: 33–38.
[2] Akinwumi, F. O. (2001). Food and feeding habits of Tilapia zilli in Ondo State University fish farm. In: Eyo A, Ajao EA (eds) Conference Proceedings of Fisheries Society of Nigeria, Maiduguri, Nigeria, pp 12–18.
[3] Ajiboye, O. O. and Yakubu, A. F. (2010). Some aspects of biology and aquaculture potentials of Tilapia guineensis (dumeril) in Nigeria. Review of Fish Biology and Fisheries 20: 441–455.
[4] Shinkafi, S. A and Ukwaja, V. C. (2010). Bacteria Associated with Fresh Tilapia Fish (Oreochromis niloticus) Sold at Sokoto Central Market in Sokoto, Nigeria. Nigerian Journal of Basic and Applied Science 18 (2): 217-221.
[5] De Sousa, J. A., Silva-Souza, A. T. (2001). Bacterial community associated with fish hand water from Congonhas River, Sertneja, parana, Brazil. Brazilian Archives of biology and Technology 44: 373-381.
[6] Trust, T. J, and Sparrow, R. A. H. (1974). The bacterial flora in the alimentary tract of freshwater salmonid fish. Canadian Journal of Microbiology 20: 1219–1228.
[7] Fredickson, B. L., Brown, S., Cohn, M. A., Conway, A., Crosby, C., McGovern, M. and Mikels, J. (2004). 5th annual meeting of society for personality and social psychology Austin, Texas on Finding positive meaning and experiencing positive emotions build resilience. Symposium presented at the functional significance of positive emotions; 29th -31st January, pp 6.
[8] Ashbolt, N. J., Grabow, W. O. K, and Snozzi, M. (2001). Indicators of microbial water quality. In: Fewtrell L, Bartram J, eds. Water quality: Guidelines, standards and health – Assessment of risk and risk management for water-related infectious disease. WHO Water Series. London, IWA Publishing, 34. 289–315.
[9] Abidemi-Iromini, A. O. and Fofah, O. J. (2016). Bacteria Occurrence in Sarotherodon malarotheron and Chrysichthys nigrodigitatus in Brackish Water Environment. Applied Tropical Agriculture 21 (1): 1- 6.
[10] Sheyin, A. N and Solomon, K. J. (2017). EndoMicrobial Fauna of Tilapia spp. (Oreochromis niloticus) found in a flowing Canal at Eden Garden and Park Utako, Abuja. Journal of Fisheries and Livestock Production 5 (1): 1 – 8.
[11] Bergey, R. E; Buchanan and Gribbons, N. E. (1974). Bergey’s Manual of Determinative Bacteriology (8th edition). Williams and Wilkins Co. Baltimore USA.
[12] Al-Harbi, A. H., and Uddin, M. N. (2007): Seasonal Trends in Gill Bacterial Flora of Hybrid Tilapia, Oreochromis niloticus × O. aureus. Journal of Applied Aquaculture 19: 1: 61-70.
[13] Nganou Donkeng, N., Maiwore, J., Tatsadjieu, L., Ngoune, D., Montet, C. and Mbofung, M. F. (2011). Characterization of the bacterial flora of tilapia (Oreochoromis niloticus) harvested from four lakes in the north of Cameroon. African Journal of Biotechnology 10 (71): 16016-16023.
[14] Al-Harbi, A. H. and Uddin, M. N. (2005). Bacterial diversity of tilapia (Oreochromis niloticus) cultured in brackish water in Saudi Arabia. Aquaculture 250: 566-572.
[15] Chessbrought, J. T. (2006) Characterization of Bacteria in fishes. Journal of Applied Science Research in the Tropics 7: 78-82.
[16] Al-Harbi, A. H., and Uddin, M. N. (2003). Quantitative and qualitative studies on bacterial flora of hybrid tilapia (Oreochromis niloticus × O. aureus) cultured in earthen ponds of Saudi Arabia. Aquaculture Research 34: 43-48.
[17] Ofimer (2002). Tilapia and Nile perch. Alternatives on the market of poissonblanc. http://www.ofimer.fr/PDF/obseco/11_noteshemp_e_dou ce.PDF.
Cite This Article
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    Ogbonne Fabian Chinedu, Ayaobu–Cookey Ibifubara Kalada, Abdullahi Mohammed Mansur, Matanmi Morufu Adewale. (2020). Prevalence of Bacterial Microbiota in Tilapia Guineensis Harvested from Buguma Brackish Water Environment River State, Nigeria. American Journal of Bioscience and Bioengineering, 8(3), 36-40. https://doi.org/10.11648/j.bio.20200803.11

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    ACS Style

    Ogbonne Fabian Chinedu; Ayaobu–Cookey Ibifubara Kalada; Abdullahi Mohammed Mansur; Matanmi Morufu Adewale. Prevalence of Bacterial Microbiota in Tilapia Guineensis Harvested from Buguma Brackish Water Environment River State, Nigeria. Am. J. BioSci. Bioeng. 2020, 8(3), 36-40. doi: 10.11648/j.bio.20200803.11

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    AMA Style

    Ogbonne Fabian Chinedu, Ayaobu–Cookey Ibifubara Kalada, Abdullahi Mohammed Mansur, Matanmi Morufu Adewale. Prevalence of Bacterial Microbiota in Tilapia Guineensis Harvested from Buguma Brackish Water Environment River State, Nigeria. Am J BioSci Bioeng. 2020;8(3):36-40. doi: 10.11648/j.bio.20200803.11

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  • @article{10.11648/j.bio.20200803.11,
      author = {Ogbonne Fabian Chinedu and Ayaobu–Cookey Ibifubara Kalada and Abdullahi Mohammed Mansur and Matanmi Morufu Adewale},
      title = {Prevalence of Bacterial Microbiota in Tilapia Guineensis Harvested from Buguma Brackish Water Environment River State, Nigeria},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {8},
      number = {3},
      pages = {36-40},
      doi = {10.11648/j.bio.20200803.11},
      url = {https://doi.org/10.11648/j.bio.20200803.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20200803.11},
      abstract = {Brackish water fishes usually harbour human pathogenic bacteria particularly the enterics and coliform groups in their mucosal surfaces. Thus, this study was undertaken to investigate the prevalence of skin and gills microbiota of Tilapia guineensis as to understand their microbial assemblages and as well provide useful insights into the broad dynamics of the fish host-microbial relationship and health status. Thirty samples of Tilapia guineensis were collected from Buguma brackish water environment, River State, Nigeria. The skin and the gills of the tilapia were analyzed microbiologically. Specimen were collected by swabbing aseptically over the skin and gills segment of the fish and then processed for total bacterial count, followed by isolation and identification of isolated bacteria. Microbial load analysis revealed that the total viable count of bacteria ranged from 2.4 to 7.6 x 105 cfu/ml for skin and 2.1 to 5.7 x 106 cfu/ml for gills, and it was observed to fall within the recommended microbiological standard safety limit. A total of thirty-seven (37) bacteria, belonging to fifteen different genera in the family of Vibrionaceae, enterobacteriaceae and Morganellaceae were isolated from the fish. The prevalent rate of the bacterial shows that Proteus vulgaris recorded the highest frequency of occurrence of 16.22%, followed by Vibrio parahaemolyticus with 10.81% occurrence and Provindencia rettgeri, Serratia mercescens and Salmonella sp. trailed them with 8.11%. Kluyyera ascorbate, Citrobacter amalonaticus, Proteus mirabilis, Enterobacter aerogenes, plesiomonas shigelloides and Pseudomonas sp. have the least frequency of occurrence with 2.7% respectively for each. Though the bacterial count of Tilapia guineensis harvested from the water and fish sample did not exceed the standard recommended safety limit. There were several potential pathogenic and spoilage bacteria isolated as part of the fish flora. The presence of these bacteria could poses or constitute a public health risk.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Prevalence of Bacterial Microbiota in Tilapia Guineensis Harvested from Buguma Brackish Water Environment River State, Nigeria
    AU  - Ogbonne Fabian Chinedu
    AU  - Ayaobu–Cookey Ibifubara Kalada
    AU  - Abdullahi Mohammed Mansur
    AU  - Matanmi Morufu Adewale
    Y1  - 2020/06/17
    PY  - 2020
    N1  - https://doi.org/10.11648/j.bio.20200803.11
    DO  - 10.11648/j.bio.20200803.11
    T2  - American Journal of Bioscience and Bioengineering
    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
    SP  - 36
    EP  - 40
    PB  - Science Publishing Group
    SN  - 2328-5893
    UR  - https://doi.org/10.11648/j.bio.20200803.11
    AB  - Brackish water fishes usually harbour human pathogenic bacteria particularly the enterics and coliform groups in their mucosal surfaces. Thus, this study was undertaken to investigate the prevalence of skin and gills microbiota of Tilapia guineensis as to understand their microbial assemblages and as well provide useful insights into the broad dynamics of the fish host-microbial relationship and health status. Thirty samples of Tilapia guineensis were collected from Buguma brackish water environment, River State, Nigeria. The skin and the gills of the tilapia were analyzed microbiologically. Specimen were collected by swabbing aseptically over the skin and gills segment of the fish and then processed for total bacterial count, followed by isolation and identification of isolated bacteria. Microbial load analysis revealed that the total viable count of bacteria ranged from 2.4 to 7.6 x 105 cfu/ml for skin and 2.1 to 5.7 x 106 cfu/ml for gills, and it was observed to fall within the recommended microbiological standard safety limit. A total of thirty-seven (37) bacteria, belonging to fifteen different genera in the family of Vibrionaceae, enterobacteriaceae and Morganellaceae were isolated from the fish. The prevalent rate of the bacterial shows that Proteus vulgaris recorded the highest frequency of occurrence of 16.22%, followed by Vibrio parahaemolyticus with 10.81% occurrence and Provindencia rettgeri, Serratia mercescens and Salmonella sp. trailed them with 8.11%. Kluyyera ascorbate, Citrobacter amalonaticus, Proteus mirabilis, Enterobacter aerogenes, plesiomonas shigelloides and Pseudomonas sp. have the least frequency of occurrence with 2.7% respectively for each. Though the bacterial count of Tilapia guineensis harvested from the water and fish sample did not exceed the standard recommended safety limit. There were several potential pathogenic and spoilage bacteria isolated as part of the fish flora. The presence of these bacteria could poses or constitute a public health risk.
    VL  - 8
    IS  - 3
    ER  - 

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Author Information
  • Department of Aquaculture, Nigerian Institute for Oceanography and Marine Research, Victoria Island, Lagos, Nigeria

  • Department of Aquaculture, Nigerian Institute for Oceanography and Marine Research, Victoria Island, Lagos, Nigeria

  • Department of Aquaculture, Nigerian Institute for Oceanography and Marine Research, Victoria Island, Lagos, Nigeria

  • Department of Aquaculture, Nigerian Institute for Oceanography and Marine Research, Victoria Island, Lagos, Nigeria

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