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Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine

Received: 30 July 2015     Accepted: 10 August 2015     Published: 19 August 2015
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Abstract

Zidovudine is a drugs used in the management of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) infection in sub-Saharan Africa in combination with other drugs. The objective of this research was to investigate the potential harmful effects of this drug on the histology of the cerebellum of Wistar rats. Twenty male Wistar rats were used for this study. The rats were divided into 2 groups of 10 rats each. Group A served as the control and was administered with 1 ml of distill water, while group B was administered with 8.57mg/kg of zidovudine daily for 30 days, after which the rats where sacrificed and each cerebellum was harvested, processed and stained using haematoxylin and Eosin (H/E), silver impregnation method. Paraffin impregnated Glial Fibrilar Acidic Protein (GFAP), Neuron Specific Enolase (NSE) and Neurofilament (NF) immunochemistry methods. Stained slides were viewed using light microscope. Results showed that, the cerebellum of Groups B animals were affected with moderate to severe shrinking and distortion of the Purkinje cells and granular cells, when compared with the control. Group B animals, also showed more expression of GFAP, NSE and NF staining in their cerebellum than the control. This suggests that zidovudine is harmful to the cerebellum and should be taken with caution

Published in American Journal of Bioscience and Bioengineering (Volume 3, Issue 3)
DOI 10.11648/j.bio.20150303.11
Page(s) 17-21
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Zidovudine, Cerebellum, Human Immunodeficiency Virus

References
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    Aniekan Imo Peter, Gabriel Joseph Ekandem, Anozeng Oyono Igiri, Moses Bassey Ekong, Dianabasi King Udoh. (2015). Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. American Journal of Bioscience and Bioengineering, 3(3), 17-21. https://doi.org/10.11648/j.bio.20150303.11

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    Aniekan Imo Peter; Gabriel Joseph Ekandem; Anozeng Oyono Igiri; Moses Bassey Ekong; Dianabasi King Udoh. Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. Am. J. BioSci. Bioeng. 2015, 3(3), 17-21. doi: 10.11648/j.bio.20150303.11

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    AMA Style

    Aniekan Imo Peter, Gabriel Joseph Ekandem, Anozeng Oyono Igiri, Moses Bassey Ekong, Dianabasi King Udoh. Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. Am J BioSci Bioeng. 2015;3(3):17-21. doi: 10.11648/j.bio.20150303.11

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  • @article{10.11648/j.bio.20150303.11,
      author = {Aniekan Imo Peter and Gabriel Joseph Ekandem and Anozeng Oyono Igiri and Moses Bassey Ekong and Dianabasi King Udoh},
      title = {Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {3},
      number = {3},
      pages = {17-21},
      doi = {10.11648/j.bio.20150303.11},
      url = {https://doi.org/10.11648/j.bio.20150303.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150303.11},
      abstract = {Zidovudine is a drugs used in the management of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) infection in sub-Saharan Africa in combination with other drugs. The objective of this research was to investigate the potential harmful effects of this drug on the histology of the cerebellum of Wistar rats. Twenty male Wistar rats were used for this study. The rats were divided into 2 groups of 10 rats each. Group A served as the control and was administered with 1 ml of distill water, while group B was administered with 8.57mg/kg of zidovudine daily for 30 days, after which the rats where sacrificed and each cerebellum was harvested, processed and stained using haematoxylin and Eosin (H/E), silver impregnation method. Paraffin impregnated Glial Fibrilar Acidic Protein (GFAP), Neuron Specific Enolase (NSE) and Neurofilament (NF) immunochemistry methods. Stained slides were viewed using light microscope. Results showed that, the cerebellum of Groups B animals were affected with moderate to severe shrinking and distortion of the Purkinje cells and granular cells, when compared with the control. Group B animals, also showed more expression of GFAP, NSE and NF staining in their cerebellum than the control. This suggests that zidovudine is harmful to the cerebellum and should be taken with caution},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine
    AU  - Aniekan Imo Peter
    AU  - Gabriel Joseph Ekandem
    AU  - Anozeng Oyono Igiri
    AU  - Moses Bassey Ekong
    AU  - Dianabasi King Udoh
    Y1  - 2015/08/19
    PY  - 2015
    N1  - https://doi.org/10.11648/j.bio.20150303.11
    DO  - 10.11648/j.bio.20150303.11
    T2  - American Journal of Bioscience and Bioengineering
    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
    SP  - 17
    EP  - 21
    PB  - Science Publishing Group
    SN  - 2328-5893
    UR  - https://doi.org/10.11648/j.bio.20150303.11
    AB  - Zidovudine is a drugs used in the management of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) infection in sub-Saharan Africa in combination with other drugs. The objective of this research was to investigate the potential harmful effects of this drug on the histology of the cerebellum of Wistar rats. Twenty male Wistar rats were used for this study. The rats were divided into 2 groups of 10 rats each. Group A served as the control and was administered with 1 ml of distill water, while group B was administered with 8.57mg/kg of zidovudine daily for 30 days, after which the rats where sacrificed and each cerebellum was harvested, processed and stained using haematoxylin and Eosin (H/E), silver impregnation method. Paraffin impregnated Glial Fibrilar Acidic Protein (GFAP), Neuron Specific Enolase (NSE) and Neurofilament (NF) immunochemistry methods. Stained slides were viewed using light microscope. Results showed that, the cerebellum of Groups B animals were affected with moderate to severe shrinking and distortion of the Purkinje cells and granular cells, when compared with the control. Group B animals, also showed more expression of GFAP, NSE and NF staining in their cerebellum than the control. This suggests that zidovudine is harmful to the cerebellum and should be taken with caution
    VL  - 3
    IS  - 3
    ER  - 

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Author Information
  • Department of Anatomy, University of Uyo, Uyo, Nigeria

  • Department of Anatomy, University of Uyo, Uyo, Nigeria

  • Department of Anatomy, University of Calabar, Uyo, Nigeriap

  • Department of Anatomy, University of Uyo, Uyo, Nigeria

  • Chemical Pathology Unit, Med Lab Services, University of Uyo Teaching Hospital, Uyo, Nigeria

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