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Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria

Received: 23 December 2014     Accepted: 12 January 2015     Published: 28 January 2015
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Abstract

Food-borne salmonellosis is the most prevalent disease and major source of Salmonella spp in humans and its detection particularly in developing countries is quite cumbersome and time consuming. Molecular methods for its detection as well as the genotypic diversity of some of the genes responsible for Salmonella virulence are necessary. The aim of the study was to screen for Salmonella spp using the 16S rRNA, to determine whether the invA gene is specific for Salmonella detection as well as virulence genotyping of some genes present in Salmonella spp (invA, sitC and spvA, spvB and spvC) from food samples in Lagos, Nigeria. All 76 isolates tested positive for 16S rRNA gene while 53 (69.7%) were positive for salm3 and salm4 (389 bp) gene. PCR analysis of the invA gene (284bp) showed that 73 (96.1%) were positive, 38 (50%) of the isolates were positive for sitC gene while none were positive for spvA and spvB and with the multiplex –PCR of invA/spvC gene 25 (33%) were positive for invA (244 bp) gene and none positive for spvC gene. The use of invA gene for Salmonella detection in our food samples is recommended however for most of our isolates the virulence genes were not detected.

Published in Animal and Veterinary Sciences (Volume 3, Issue 1)
DOI 10.11648/j.avs.20150301.15
Page(s) 22-27
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Detection, Food Samples, PCR, Salmonella, Virulence Genes

References
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Cite This Article
  • APA Style

    Stella Ifeanyi Smith, Muinah Adenike Fowora, Adedamilola Atiba, Joseph Anejo-Okopi, Tina Fingesi, et al. (2015). Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria. Animal and Veterinary Sciences, 3(1), 22-27. https://doi.org/10.11648/j.avs.20150301.15

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    ACS Style

    Stella Ifeanyi Smith; Muinah Adenike Fowora; Adedamilola Atiba; Joseph Anejo-Okopi; Tina Fingesi, et al. Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria. Anim. Vet. Sci. 2015, 3(1), 22-27. doi: 10.11648/j.avs.20150301.15

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    AMA Style

    Stella Ifeanyi Smith, Muinah Adenike Fowora, Adedamilola Atiba, Joseph Anejo-Okopi, Tina Fingesi, et al. Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria. Anim Vet Sci. 2015;3(1):22-27. doi: 10.11648/j.avs.20150301.15

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  • @article{10.11648/j.avs.20150301.15,
      author = {Stella Ifeanyi Smith and Muinah Adenike Fowora and Adedamilola Atiba and Joseph Anejo-Okopi and Tina Fingesi and Mary Ehi Adamu and Emmanuel Adedayo Omonigbehin and Margaret Iteun Ugo-Ijeh and Moses Bamidele and Peter Odeigah},
      title = {Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria},
      journal = {Animal and Veterinary Sciences},
      volume = {3},
      number = {1},
      pages = {22-27},
      doi = {10.11648/j.avs.20150301.15},
      url = {https://doi.org/10.11648/j.avs.20150301.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150301.15},
      abstract = {Food-borne salmonellosis is the most prevalent disease and major source of Salmonella spp in humans and its detection particularly in developing countries is quite cumbersome and time consuming. Molecular methods for its detection as well as the genotypic diversity of some of the genes responsible for Salmonella virulence are necessary. The aim of the study was to screen for Salmonella spp using the 16S rRNA, to determine whether the invA gene is specific for Salmonella detection as well as virulence genotyping of some genes present in Salmonella spp (invA, sitC and spvA, spvB and spvC) from food samples in Lagos, Nigeria. All 76 isolates tested positive for 16S rRNA gene while 53 (69.7%) were positive for salm3 and salm4 (389 bp) gene. PCR analysis of the invA gene (284bp) showed that 73 (96.1%) were positive, 38 (50%) of the isolates were positive for sitC gene while none were positive for spvA and spvB and with the multiplex –PCR of invA/spvC gene 25 (33%) were positive for invA (244 bp) gene and none positive for spvC gene. The use of invA gene for Salmonella detection in our food samples is recommended however for most of our isolates the virulence genes were not detected.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria
    AU  - Stella Ifeanyi Smith
    AU  - Muinah Adenike Fowora
    AU  - Adedamilola Atiba
    AU  - Joseph Anejo-Okopi
    AU  - Tina Fingesi
    AU  - Mary Ehi Adamu
    AU  - Emmanuel Adedayo Omonigbehin
    AU  - Margaret Iteun Ugo-Ijeh
    AU  - Moses Bamidele
    AU  - Peter Odeigah
    Y1  - 2015/01/28
    PY  - 2015
    N1  - https://doi.org/10.11648/j.avs.20150301.15
    DO  - 10.11648/j.avs.20150301.15
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
    SP  - 22
    EP  - 27
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20150301.15
    AB  - Food-borne salmonellosis is the most prevalent disease and major source of Salmonella spp in humans and its detection particularly in developing countries is quite cumbersome and time consuming. Molecular methods for its detection as well as the genotypic diversity of some of the genes responsible for Salmonella virulence are necessary. The aim of the study was to screen for Salmonella spp using the 16S rRNA, to determine whether the invA gene is specific for Salmonella detection as well as virulence genotyping of some genes present in Salmonella spp (invA, sitC and spvA, spvB and spvC) from food samples in Lagos, Nigeria. All 76 isolates tested positive for 16S rRNA gene while 53 (69.7%) were positive for salm3 and salm4 (389 bp) gene. PCR analysis of the invA gene (284bp) showed that 73 (96.1%) were positive, 38 (50%) of the isolates were positive for sitC gene while none were positive for spvA and spvB and with the multiplex –PCR of invA/spvC gene 25 (33%) were positive for invA (244 bp) gene and none positive for spvC gene. The use of invA gene for Salmonella detection in our food samples is recommended however for most of our isolates the virulence genes were not detected.
    VL  - 3
    IS  - 1
    ER  - 

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Author Information
  • Molecular Biology and Biotechnology division, Nigerian Institute of Medical Research Yaba, Lagos, Nigeria

  • Molecular Biology and Biotechnology division, Nigerian Institute of Medical Research Yaba, Lagos, Nigeria

  • Cell Biology and Genetics department, University of Lagos, Akoka, Lagos, Nigeria

  • Microbiology department, University of Jos/Jos University Teaching Hospital, AIDS Prevention Initiative in Nigeria department, Jos, Nigeria

  • Cell Biology and Genetics department, University of Lagos, Akoka, Lagos, Nigeria

  • Cell Biology and Genetics department, University of Lagos, Akoka, Lagos, Nigeria

  • Molecular Biology and Biotechnology division, Nigerian Institute of Medical Research Yaba, Lagos, Nigeria

  • Molecular Biology and Biotechnology division, Nigerian Institute of Medical Research Yaba, Lagos, Nigeria

  • Cell Biology and Genetics department, University of Lagos, Akoka, Lagos, Nigeria

  • Cell Biology and Genetics department, University of Lagos, Akoka, Lagos, Nigeria

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