Advances in Surgical Sciences

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Study on Prp-Induced Chondrogenesis of Adipose Stem Cells

Received: Sep. 27, 2023    Accepted: Oct. 13, 2023    Published: Oct. 28, 2023
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Abstract

Background: In the field of repair and reconstruction surgery, the use of cartilage is large, but the source of cartilage is small; adipose stem cells come from a wide range of sources and can be induced to differentiate into a variety of cells under specific circumstances. By using platelet-rich plasma to induce differentiation of adipose stem cells into chondrocytes, tissue engineering construction of cartilage will become possible. Objective: The optimal culture conditions for PRP-induced differentiation of adipose stem cells into chondrocytes were screened, and REAL-TIME PCR detection of aggrecan and sox-9 were used to confirm that adipose stem cells can be induced by PRP to differentiate into chondrocytes. Methods: After isolating and culturing adipose stem cells, use DMEM culture medium containing 0, 5% and 10% PRP to subculture the cells to induce differentiation. The CCK8 kit detects cell proliferation; use DMEM culture medium containing 5% PRP to culture adipose stem cells. Cells were collected on days 3, 7, 14 and 21 of culture, and Real-Time PCR were performed to detect specific antigens aggrecan and sox-9. Results: In the DMEM culture medium containing 5% PRP, adipose stem cells grow well and are not prone to aggregation reactions. On the 3rd, 7th, 14th and 21st days of differentiation induced by 5% PRP, Real-Time PCR tests found that aggrecan and sox-9 were continuously expressed, and their expression was found to be progressively enhanced. Conclusion: DMEM medium containing 5% PRP is a suitable medium for inducing the differentiation of adipose stem cells into chondrocytes. Adipose stem cells can be used as seed cells to differentiate into chondrocytes after being induced by PRP and have the characteristic antigen expression of chondrocytes.

DOI 10.11648/j.ass.20231102.13
Published in Advances in Surgical Sciences ( Volume 11, Issue 2, December 2023 )
Page(s) 32-35
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Platelet-Rich Plasma (Prp), Adipose Stem Cells (Adsc), Chondrocytes, Aggrecan, Sox-9

References
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  • APA Style

    Lun Liu, Bin Yang, Bin Xu. (2023). Study on Prp-Induced Chondrogenesis of Adipose Stem Cells. Advances in Surgical Sciences, 11(2), 32-35. https://doi.org/10.11648/j.ass.20231102.13

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    ACS Style

    Lun Liu; Bin Yang; Bin Xu. Study on Prp-Induced Chondrogenesis of Adipose Stem Cells. Adv. Surg. Sci. 2023, 11(2), 32-35. doi: 10.11648/j.ass.20231102.13

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    AMA Style

    Lun Liu, Bin Yang, Bin Xu. Study on Prp-Induced Chondrogenesis of Adipose Stem Cells. Adv Surg Sci. 2023;11(2):32-35. doi: 10.11648/j.ass.20231102.13

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  • @article{10.11648/j.ass.20231102.13,
      author = {Lun Liu and Bin Yang and Bin Xu},
      title = {Study on Prp-Induced Chondrogenesis of Adipose Stem Cells},
      journal = {Advances in Surgical Sciences},
      volume = {11},
      number = {2},
      pages = {32-35},
      doi = {10.11648/j.ass.20231102.13},
      url = {https://doi.org/10.11648/j.ass.20231102.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ass.20231102.13},
      abstract = {Background: In the field of repair and reconstruction surgery, the use of cartilage is large, but the source of cartilage is small; adipose stem cells come from a wide range of sources and can be induced to differentiate into a variety of cells under specific circumstances. By using platelet-rich plasma to induce differentiation of adipose stem cells into chondrocytes, tissue engineering construction of cartilage will become possible. Objective: The optimal culture conditions for PRP-induced differentiation of adipose stem cells into chondrocytes were screened, and REAL-TIME PCR detection of aggrecan and sox-9 were used to confirm that adipose stem cells can be induced by PRP to differentiate into chondrocytes. Methods: After isolating and culturing adipose stem cells, use DMEM culture medium containing 0, 5% and 10% PRP to subculture the cells to induce differentiation. The CCK8 kit detects cell proliferation; use DMEM culture medium containing 5% PRP to culture adipose stem cells. Cells were collected on days 3, 7, 14 and 21 of culture, and Real-Time PCR were performed to detect specific antigens aggrecan and sox-9. Results: In the DMEM culture medium containing 5% PRP, adipose stem cells grow well and are not prone to aggregation reactions. On the 3rd, 7th, 14th and 21st days of differentiation induced by 5% PRP, Real-Time PCR tests found that aggrecan and sox-9 were continuously expressed, and their expression was found to be progressively enhanced. Conclusion: DMEM medium containing 5% PRP is a suitable medium for inducing the differentiation of adipose stem cells into chondrocytes. Adipose stem cells can be used as seed cells to differentiate into chondrocytes after being induced by PRP and have the characteristic antigen expression of chondrocytes.
    },
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Study on Prp-Induced Chondrogenesis of Adipose Stem Cells
    AU  - Lun Liu
    AU  - Bin Yang
    AU  - Bin Xu
    Y1  - 2023/10/28
    PY  - 2023
    N1  - https://doi.org/10.11648/j.ass.20231102.13
    DO  - 10.11648/j.ass.20231102.13
    T2  - Advances in Surgical Sciences
    JF  - Advances in Surgical Sciences
    JO  - Advances in Surgical Sciences
    SP  - 32
    EP  - 35
    PB  - Science Publishing Group
    SN  - 2376-6182
    UR  - https://doi.org/10.11648/j.ass.20231102.13
    AB  - Background: In the field of repair and reconstruction surgery, the use of cartilage is large, but the source of cartilage is small; adipose stem cells come from a wide range of sources and can be induced to differentiate into a variety of cells under specific circumstances. By using platelet-rich plasma to induce differentiation of adipose stem cells into chondrocytes, tissue engineering construction of cartilage will become possible. Objective: The optimal culture conditions for PRP-induced differentiation of adipose stem cells into chondrocytes were screened, and REAL-TIME PCR detection of aggrecan and sox-9 were used to confirm that adipose stem cells can be induced by PRP to differentiate into chondrocytes. Methods: After isolating and culturing adipose stem cells, use DMEM culture medium containing 0, 5% and 10% PRP to subculture the cells to induce differentiation. The CCK8 kit detects cell proliferation; use DMEM culture medium containing 5% PRP to culture adipose stem cells. Cells were collected on days 3, 7, 14 and 21 of culture, and Real-Time PCR were performed to detect specific antigens aggrecan and sox-9. Results: In the DMEM culture medium containing 5% PRP, adipose stem cells grow well and are not prone to aggregation reactions. On the 3rd, 7th, 14th and 21st days of differentiation induced by 5% PRP, Real-Time PCR tests found that aggrecan and sox-9 were continuously expressed, and their expression was found to be progressively enhanced. Conclusion: DMEM medium containing 5% PRP is a suitable medium for inducing the differentiation of adipose stem cells into chondrocytes. Adipose stem cells can be used as seed cells to differentiate into chondrocytes after being induced by PRP and have the characteristic antigen expression of chondrocytes.
    
    VL  - 11
    IS  - 2
    ER  - 

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Author Information
  • Department of Plastic Surgery, Yangzhong People’s Hospital Affiliated to Yangzhou University Medical Academy, Yangzhong City, China

  • Department of Plastic Surgery, Yangzhong People’s Hospital Affiliated to Yangzhou University Medical Academy, Yangzhong City, China

  • Department of Plastic Surgery, Yangzhong People’s Hospital Affiliated to Yangzhou University Medical Academy, Yangzhong City, China

  • Section