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Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance

Received: 5 July 2017     Accepted: 14 July 2017     Published: 7 August 2017
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Abstract

Objective to investigate the expression of NF-κB and occludin in intestinal mucosal barrier during severe acute pancreatitis (SAP) and its clinical significance. Method Twenty-four Wistar rats were randomly divided into a normal control group, an SAP group, and a PDTC group. The rats were sacrificed at 24h after modeling, and general appearance of the abdominal cavity, pathological changes of the intestinal mucosa, changes in intestinal mucosal permeability were observed, serum inflammatory factor IL-1 was measured, and expression of tight junction protein occludin in the intestinal epithelial cells were determined by immunohistochemistry. Results Rats of the SAP group showed obvious inflammatory reaction in the abdominal cavity, manifested by enlargement of intestinal tract, intestinal edema, and increased permeability of intestinal barrier, whereas NF-κB inhibitor alleviated intestinal damage and intestinal mucosa edema, upregulated intestinal epithelial tight junction protein occludin, decreased intestinal permeability, and lowered the level of inflammatory factors. Conclusion Impairment of intestinal barrier function during SAP may be associated with increased NF-κB and decreased intestinal tight junction protein occludin; inhibiting NF-kB may alleviate intestinal mucosal barrier dysfunction during SAP.

Published in American Journal of Internal Medicine (Volume 5, Issue 5)
DOI 10.11648/j.ajim.20170505.11
Page(s) 70-73
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Severe Acute Pancreatitis, Intestinal Mucosal Barrier, Nf-Κb, Inflammatory Factor

References
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[14] Irene Guijarro-Muñoz, Marta Compte, Ana Álvarez-Cienfuegos, et al. Lipopolysaccharide Activates Toll-like Receptor 4 (TLR 4)-mediated NF-κB Signaling Pathway and Proinflammatory Response in Human Pericytes J Biol Chem, 2014, 289 (4): 2457–2468.
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Cite This Article
  • APA Style

    Qingyun Zhu, Shigang Yang, Xinting Pan, Chonggao Yin, Shijie Liu, et al. (2017). Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance. American Journal of Internal Medicine, 5(5), 70-73. https://doi.org/10.11648/j.ajim.20170505.11

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    ACS Style

    Qingyun Zhu; Shigang Yang; Xinting Pan; Chonggao Yin; Shijie Liu, et al. Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance. Am. J. Intern. Med. 2017, 5(5), 70-73. doi: 10.11648/j.ajim.20170505.11

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    AMA Style

    Qingyun Zhu, Shigang Yang, Xinting Pan, Chonggao Yin, Shijie Liu, et al. Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance. Am J Intern Med. 2017;5(5):70-73. doi: 10.11648/j.ajim.20170505.11

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  • @article{10.11648/j.ajim.20170505.11,
      author = {Qingyun Zhu and Shigang Yang and Xinting Pan and Chonggao Yin and Shijie Liu and Fuguo Liu and Ying Sun and Huimin Wang and Yajun Jing},
      title = {Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance},
      journal = {American Journal of Internal Medicine},
      volume = {5},
      number = {5},
      pages = {70-73},
      doi = {10.11648/j.ajim.20170505.11},
      url = {https://doi.org/10.11648/j.ajim.20170505.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20170505.11},
      abstract = {Objective to investigate the expression of NF-κB and occludin in intestinal mucosal barrier during severe acute pancreatitis (SAP) and its clinical significance. Method Twenty-four Wistar rats were randomly divided into a normal control group, an SAP group, and a PDTC group. The rats were sacrificed at 24h after modeling, and general appearance of the abdominal cavity, pathological changes of the intestinal mucosa, changes in intestinal mucosal permeability were observed, serum inflammatory factor IL-1 was measured, and expression of tight junction protein occludin in the intestinal epithelial cells were determined by immunohistochemistry. Results Rats of the SAP group showed obvious inflammatory reaction in the abdominal cavity, manifested by enlargement of intestinal tract, intestinal edema, and increased permeability of intestinal barrier, whereas NF-κB inhibitor alleviated intestinal damage and intestinal mucosa edema, upregulated intestinal epithelial tight junction protein occludin, decreased intestinal permeability, and lowered the level of inflammatory factors. Conclusion Impairment of intestinal barrier function during SAP may be associated with increased NF-κB and decreased intestinal tight junction protein occludin; inhibiting NF-kB may alleviate intestinal mucosal barrier dysfunction during SAP.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance
    AU  - Qingyun Zhu
    AU  - Shigang Yang
    AU  - Xinting Pan
    AU  - Chonggao Yin
    AU  - Shijie Liu
    AU  - Fuguo Liu
    AU  - Ying Sun
    AU  - Huimin Wang
    AU  - Yajun Jing
    Y1  - 2017/08/07
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajim.20170505.11
    DO  - 10.11648/j.ajim.20170505.11
    T2  - American Journal of Internal Medicine
    JF  - American Journal of Internal Medicine
    JO  - American Journal of Internal Medicine
    SP  - 70
    EP  - 73
    PB  - Science Publishing Group
    SN  - 2330-4324
    UR  - https://doi.org/10.11648/j.ajim.20170505.11
    AB  - Objective to investigate the expression of NF-κB and occludin in intestinal mucosal barrier during severe acute pancreatitis (SAP) and its clinical significance. Method Twenty-four Wistar rats were randomly divided into a normal control group, an SAP group, and a PDTC group. The rats were sacrificed at 24h after modeling, and general appearance of the abdominal cavity, pathological changes of the intestinal mucosa, changes in intestinal mucosal permeability were observed, serum inflammatory factor IL-1 was measured, and expression of tight junction protein occludin in the intestinal epithelial cells were determined by immunohistochemistry. Results Rats of the SAP group showed obvious inflammatory reaction in the abdominal cavity, manifested by enlargement of intestinal tract, intestinal edema, and increased permeability of intestinal barrier, whereas NF-κB inhibitor alleviated intestinal damage and intestinal mucosa edema, upregulated intestinal epithelial tight junction protein occludin, decreased intestinal permeability, and lowered the level of inflammatory factors. Conclusion Impairment of intestinal barrier function during SAP may be associated with increased NF-κB and decreased intestinal tight junction protein occludin; inhibiting NF-kB may alleviate intestinal mucosal barrier dysfunction during SAP.
    VL  - 5
    IS  - 5
    ER  - 

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Author Information
  • Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China

  • Department of Gastroenterology, The Qingzhou Huanglou Hospital, Qingzhou, China

  • Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China

  • Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China

  • Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China

  • Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China

  • Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China

  • Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China

  • Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China

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