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Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria

Received: 19 April 2017     Accepted: 26 May 2017     Published: 11 August 2017
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Abstract

Bacterial colonies can differ greatly in their morphologies. These differences can help us in identifying different species of bacteria. The clinical isolates of Staphylococcus Aureus are subjected to standard biochemical tests, to observe the biochemical characteristics. One hundred and ten (110) pathogenic Staphylococcus Aureus strains were used in this study. Characteristics of these strains were determined by biochemical tests such as catalase, coagulase, DNase test, test for beta haemolysin, fermentation of mannitol and lactose. Staphylococcus Aureus [American Type Culture Collection (ATCC) 25923] was used as a reference control organism. From this study, males were more infected than females, having 60 (61.8%) and 42 (38.2%) respectively. The age group with the highest number of isolates was (0-10) years while blood culture had the highest frequency of Staphylococcus Aureus isolates with a frequency of 42 (38.1%). It was also observed that 100% of Staphylococcus Aureus showed positive results in catalase and tube coagulase, about 101 (91.8%) were positive for slide coagulase production test, 102 (92.7%) strains were positive to DNase test, 63 (57.3%) were positive to beta haemolysin while 79 (71.8%) and 106 (96.4%) strains fermented Mannitol and lactose respectively. This study reveals that no single test that can be used for the identification of Staphylococcus Aureus, hence the need for sequel testing using proper biochemical tests should be used for the identification of Staphylococcus Aureus isolates.

Published in American Journal of Internal Medicine (Volume 5, Issue 5)
DOI 10.11648/j.ajim.20170505.12
Page(s) 74-78
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Staphylococcus Aureus, Biochemical, Characteristics, Fermentation, Identification

References
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[6] Cheesbrough M (2010). District Laboratory Practice in Tropical Countries part two, New York, Cambridge University Press U.S. pp. 64-67.
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Cite This Article
  • APA Style

    Abdulhadi Sale Kumurya, Hamza Sule, Nasidi Abba Ahmed. (2017). Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria. American Journal of Internal Medicine, 5(5), 74-78. https://doi.org/10.11648/j.ajim.20170505.12

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    ACS Style

    Abdulhadi Sale Kumurya; Hamza Sule; Nasidi Abba Ahmed. Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria. Am. J. Intern. Med. 2017, 5(5), 74-78. doi: 10.11648/j.ajim.20170505.12

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    AMA Style

    Abdulhadi Sale Kumurya, Hamza Sule, Nasidi Abba Ahmed. Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria. Am J Intern Med. 2017;5(5):74-78. doi: 10.11648/j.ajim.20170505.12

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  • @article{10.11648/j.ajim.20170505.12,
      author = {Abdulhadi Sale Kumurya and Hamza Sule and Nasidi Abba Ahmed},
      title = {Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria},
      journal = {American Journal of Internal Medicine},
      volume = {5},
      number = {5},
      pages = {74-78},
      doi = {10.11648/j.ajim.20170505.12},
      url = {https://doi.org/10.11648/j.ajim.20170505.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20170505.12},
      abstract = {Bacterial colonies can differ greatly in their morphologies. These differences can help us in identifying different species of bacteria. The clinical isolates of Staphylococcus Aureus are subjected to standard biochemical tests, to observe the biochemical characteristics. One hundred and ten (110) pathogenic Staphylococcus Aureus strains were used in this study. Characteristics of these strains were determined by biochemical tests such as catalase, coagulase, DNase test, test for beta haemolysin, fermentation of mannitol and lactose. Staphylococcus Aureus [American Type Culture Collection (ATCC) 25923] was used as a reference control organism. From this study, males were more infected than females, having 60 (61.8%) and 42 (38.2%) respectively. The age group with the highest number of isolates was (0-10) years while blood culture had the highest frequency of Staphylococcus Aureus isolates with a frequency of 42 (38.1%). It was also observed that 100% of Staphylococcus Aureus showed positive results in catalase and tube coagulase, about 101 (91.8%) were positive for slide coagulase production test, 102 (92.7%) strains were positive to DNase test, 63 (57.3%) were positive to beta haemolysin while 79 (71.8%) and 106 (96.4%) strains fermented Mannitol and lactose respectively. This study reveals that no single test that can be used for the identification of Staphylococcus Aureus, hence the need for sequel testing using proper biochemical tests should be used for the identification of Staphylococcus Aureus isolates.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria
    AU  - Abdulhadi Sale Kumurya
    AU  - Hamza Sule
    AU  - Nasidi Abba Ahmed
    Y1  - 2017/08/11
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajim.20170505.12
    DO  - 10.11648/j.ajim.20170505.12
    T2  - American Journal of Internal Medicine
    JF  - American Journal of Internal Medicine
    JO  - American Journal of Internal Medicine
    SP  - 74
    EP  - 78
    PB  - Science Publishing Group
    SN  - 2330-4324
    UR  - https://doi.org/10.11648/j.ajim.20170505.12
    AB  - Bacterial colonies can differ greatly in their morphologies. These differences can help us in identifying different species of bacteria. The clinical isolates of Staphylococcus Aureus are subjected to standard biochemical tests, to observe the biochemical characteristics. One hundred and ten (110) pathogenic Staphylococcus Aureus strains were used in this study. Characteristics of these strains were determined by biochemical tests such as catalase, coagulase, DNase test, test for beta haemolysin, fermentation of mannitol and lactose. Staphylococcus Aureus [American Type Culture Collection (ATCC) 25923] was used as a reference control organism. From this study, males were more infected than females, having 60 (61.8%) and 42 (38.2%) respectively. The age group with the highest number of isolates was (0-10) years while blood culture had the highest frequency of Staphylococcus Aureus isolates with a frequency of 42 (38.1%). It was also observed that 100% of Staphylococcus Aureus showed positive results in catalase and tube coagulase, about 101 (91.8%) were positive for slide coagulase production test, 102 (92.7%) strains were positive to DNase test, 63 (57.3%) were positive to beta haemolysin while 79 (71.8%) and 106 (96.4%) strains fermented Mannitol and lactose respectively. This study reveals that no single test that can be used for the identification of Staphylococcus Aureus, hence the need for sequel testing using proper biochemical tests should be used for the identification of Staphylococcus Aureus isolates.
    VL  - 5
    IS  - 5
    ER  - 

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Author Information
  • Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria

  • Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria

  • Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria

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