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Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis

Received: 26 December 2018     Published: 27 December 2018
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Abstract

Objecive: To study the effects of Huoxue Jiedu Jiangtang formulation (HJJF) on endoplasmic reticulum stress (ERS) coupled inflammatory response From the molecular mechanism in diabetic vascular disease. Methods: Type 2 diabetes were established in male SD rats by feeding high lipid diet and injection of streptozotocin. after T2DM models established successfully, the rats were divided into model group, low-dose HJJF group (HJJF1), high-dose HJJF group (HJJF2), and Western medicine group (Gliquidone+Benazepril), and accepted corresponding drugs for 8 weeks respectively. Another normal group were used as control group, fed with normal diet, no drug intervention. The levels of fasting blood glucose (FBG), serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), IL-6 and TNF-α were tested. The ERS signaling molecules glucose-regulated protein 78 (GRP78) and c-Jun N-terminal kinases (JNK) mRNA transcription level in thoracic aorta vessel were determined by reverse-transcription polymerase chain reaction (RT-PCR). Results: Compared with model group, After drugs intervention, all administered groups could significantly decrease FPG (P<0.05), elevate SOD and GSH-Px (P<0.05); all could reduce levels of TC, TG, LDL-C, IL-6 and TNF-α (P<0.05), and raise HDL-C (P<0.05); all could depress the transcription levels of GRP78 and JNK mRNA (P<0.05). With increasing dose, the HJJF effect is more significant (P<0.05). Conclusion: HJJF could improve insulin resistance, correct lipid metabolism disorders, and enhance the antioxidative ability, inhibit the response of ERS coupled inflammatory, showing the multi-target treatment characteristics of Traditional Chinese Medicine.

Published in American Journal of Clinical and Experimental Medicine (Volume 6, Issue 5)
DOI 10.11648/j.ajcem.20180605.13
Page(s) 118-124
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Diabetes, Atherosclerosis, Huoxue Jiedu Jiangtang Formulation, Inflammatory Reaction, Endoplasmic Reticulum Stress

References
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[3] Sanson M, Auge N, Vindis C, et al. Oxidized low-density lipoproteins trigger endoplasmic reticulum stress in vascular cells: prevention by oxygen-regulated protein 150 expression. Cire Res, 2009, 104: 328-336.
[4] FU Xianzhao, XU Jing, HUANG Wenhua, et al. Strategy of Stasis-resolving and Detoxification on Diabetic Coronary Heart Disease [J]. JE TCM, 2014, 23 (11): 2024-2027.
[5] FU Xian-zhao, XU Jing, LI Chun-yan, et al. Effects of Huoxue Jiedu Jiangtang formulation on the balance between pro- and anti-inflammatory cytokines in non-revascularization patients with diabetic acute coronary syndrome [J]. Chin JTCM WM Crit Care, 2016, 23 (1): 6-10.
[6] Doultsinos D, Avril T, Lhomond S, et al. Control of the Unfolded Protein Response in Health and Disease. SLAS Discov, 2017, 22 (7): 787-800.
[7] GUAN LiYing, XU CaiMin, PAN Hua Zhen. Endoplasmic Reticulam Stress-induced Apoptosis. Prigress in Biochemistry and Biophysics, 2007, 11: 1136-1141.
[8] Hong F, Liu B, Wu BX, et al. CNPY2 is a key initiator of the PERK-CHOP pathway of the unfolded protein response. Nat Struct Mol Biol, 2017, 24 (10): 834-839.
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[19] FU Xian-zhao, XU Jing, LI Chun-yan, et al. Effect of Huoxue Jiedu Jiangtang formulation on heart remodeling in diabetic patients with acute coronary syndrome after PCI [J]. Chin J TCM WM Crit Care, 2016, 23 (2): 117-121.
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  • APA Style

    Fu Xianzhao, Huang Wenhua, Li Chunyan, Li Xingchan, Qiu Haixian, et al. (2018). Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis. American Journal of Clinical and Experimental Medicine, 6(5), 118-124. https://doi.org/10.11648/j.ajcem.20180605.13

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    ACS Style

    Fu Xianzhao; Huang Wenhua; Li Chunyan; Li Xingchan; Qiu Haixian, et al. Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis. Am. J. Clin. Exp. Med. 2018, 6(5), 118-124. doi: 10.11648/j.ajcem.20180605.13

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    AMA Style

    Fu Xianzhao, Huang Wenhua, Li Chunyan, Li Xingchan, Qiu Haixian, et al. Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis. Am J Clin Exp Med. 2018;6(5):118-124. doi: 10.11648/j.ajcem.20180605.13

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  • @article{10.11648/j.ajcem.20180605.13,
      author = {Fu Xianzhao and Huang Wenhua and Li Chunyan and Li Xingchan and Qiu Haixian and Liang Liudan and Cao Qiuxia and Liang Liuyue},
      title = {Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {6},
      number = {5},
      pages = {118-124},
      doi = {10.11648/j.ajcem.20180605.13},
      url = {https://doi.org/10.11648/j.ajcem.20180605.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20180605.13},
      abstract = {Objecive: To study the effects of Huoxue Jiedu Jiangtang formulation (HJJF) on endoplasmic reticulum stress (ERS) coupled inflammatory response From the molecular mechanism in diabetic vascular disease. Methods: Type 2 diabetes were established in male SD rats by feeding high lipid diet and injection of streptozotocin. after T2DM models established successfully, the rats were divided into model group, low-dose HJJF group (HJJF1), high-dose HJJF group (HJJF2), and Western medicine group (Gliquidone+Benazepril), and accepted corresponding drugs for 8 weeks respectively. Another normal group were used as control group, fed with normal diet, no drug intervention. The levels of fasting blood glucose (FBG), serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), IL-6 and TNF-α were tested. The ERS signaling molecules glucose-regulated protein 78 (GRP78) and c-Jun N-terminal kinases (JNK) mRNA transcription level in thoracic aorta vessel were determined by reverse-transcription polymerase chain reaction (RT-PCR). Results: Compared with model group, After drugs intervention, all administered groups could significantly decrease FPG (P<0.05), elevate SOD and GSH-Px (P<0.05); all could reduce levels of TC, TG, LDL-C, IL-6 and TNF-α (P<0.05), and raise HDL-C (P<0.05); all could depress the transcription levels of GRP78 and JNK mRNA (P<0.05). With increasing dose, the HJJF effect is more significant (P<0.05). Conclusion: HJJF could improve insulin resistance, correct lipid metabolism disorders, and enhance the antioxidative ability, inhibit the response of ERS coupled inflammatory, showing the multi-target treatment characteristics of Traditional Chinese Medicine.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis
    AU  - Fu Xianzhao
    AU  - Huang Wenhua
    AU  - Li Chunyan
    AU  - Li Xingchan
    AU  - Qiu Haixian
    AU  - Liang Liudan
    AU  - Cao Qiuxia
    AU  - Liang Liuyue
    Y1  - 2018/12/27
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ajcem.20180605.13
    DO  - 10.11648/j.ajcem.20180605.13
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 118
    EP  - 124
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20180605.13
    AB  - Objecive: To study the effects of Huoxue Jiedu Jiangtang formulation (HJJF) on endoplasmic reticulum stress (ERS) coupled inflammatory response From the molecular mechanism in diabetic vascular disease. Methods: Type 2 diabetes were established in male SD rats by feeding high lipid diet and injection of streptozotocin. after T2DM models established successfully, the rats were divided into model group, low-dose HJJF group (HJJF1), high-dose HJJF group (HJJF2), and Western medicine group (Gliquidone+Benazepril), and accepted corresponding drugs for 8 weeks respectively. Another normal group were used as control group, fed with normal diet, no drug intervention. The levels of fasting blood glucose (FBG), serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), IL-6 and TNF-α were tested. The ERS signaling molecules glucose-regulated protein 78 (GRP78) and c-Jun N-terminal kinases (JNK) mRNA transcription level in thoracic aorta vessel were determined by reverse-transcription polymerase chain reaction (RT-PCR). Results: Compared with model group, After drugs intervention, all administered groups could significantly decrease FPG (P<0.05), elevate SOD and GSH-Px (P<0.05); all could reduce levels of TC, TG, LDL-C, IL-6 and TNF-α (P<0.05), and raise HDL-C (P<0.05); all could depress the transcription levels of GRP78 and JNK mRNA (P<0.05). With increasing dose, the HJJF effect is more significant (P<0.05). Conclusion: HJJF could improve insulin resistance, correct lipid metabolism disorders, and enhance the antioxidative ability, inhibit the response of ERS coupled inflammatory, showing the multi-target treatment characteristics of Traditional Chinese Medicine.
    VL  - 6
    IS  - 5
    ER  - 

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Author Information
  • Clinic Medical College of Youjiang Medical National College, Baise, China

  • Clinic Medical College of Youjiang Medical National College, Baise, China

  • Clinic Medical College of Youjiang Medical National College, Baise, China

  • Clinic Medical College of Youjiang Medical National College, Baise, China

  • Clinic Medical College of Youjiang Medical National College, Baise, China

  • Clinic Medical College of Youjiang Medical National College, Baise, China

  • Clinic Medical College of Youjiang Medical National College, Baise, China

  • Clinic Medical College of Youjiang Medical National College, Baise, China

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