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Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM

Received: 17 October 2018     Published: 18 October 2018
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Abstract

Aim: The HFMD caused by EV71 virus has extremely high lethality and morbidity. EV71's pathogenesis has not understood thoroughly and the antiviral drug has been under development, which led to its emergence as a clinically fatal neurotropic virus. Early diagnosis is one of the main methods to reduce mortality. The aim of this study was to establish and evaluate a colloidal gold immunochromatography for qualitatively detecting the EV71-IgM in serum sample. Method: In this study, the different kinds of coating antigen were the focus of optimization. The pH value, particle size of the colloidal gold and other parameters and materials were also considered to achieve the good performance in detecting strips. The CA16-positive, EV71-positive and negative serum were used to compare and evaluate the accuracy and specificity of strips. Result: The accuracy of the strips coated by EV71-VP1-VP2-VP3 was higher than the EV71-VP1 strips 20%. The specificity of the EV71-VP1 strips was slightly higher than EV71-VP1-VP2-VP3 strips according to CA16 positive serum samples. Compared with commercial strips, this diagnostic method just needed 2 µL serum samples for detection, and the accuracy of the EV71-VP1-VP2-VP3 strips was significantly higher than the commercial strips. Conclusion: The developed method established in this study can apply to early diagnosis of HFMD caused by EV71 virus.

Published in American Journal of Clinical and Experimental Medicine (Volume 6, Issue 4)
DOI 10.11648/j.ajcem.20180604.13
Page(s) 99-102
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

HFMD, EV71-IgM, Colloidal Gold

References
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[2] Kan X W, Justin J C. Antiviral screen identifies EV71 inhibitors and reveals camptothecin-target, DNA topoisomerase 1 as a novel EV71 host factor. Antiviral Research 2017; 143: 122-133.
[3] Ma H Y, Lu C Y, Tsao K C, et al. Association of EV71 3C polymorphisms with clinical severity. Journal of Microbiology, Immunology and Infection 2017; 28: 1-6.
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[7] Schmidt N J, Lennette E H, Ho H H. An apparently new enterovirus isolated from patients with disease of the central nervous system. J Infect Dis 1974; 129: 304-309.
[8] Xing W, Liao Q, Viboud C, et al. Hand, foot, and mouth disease in China, 2008-12: an epidemiological study. Lancet Infect Dis 2014; 14(4): 308-318.
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[10] Liu C C, Chang H W, Yang G, et al. Development of a quantitative enzyme linked immunosorbent assay for monitoring the Enterovirus 71 vaccine manufacturing process. Journal of Virological Methods. 2011; 176 (1-2): 60-68.
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[14] Sunita S, Chow V T K, Phoon M C, et al. Direct Detection of Enterovirus 71 (EV71) in Clinical Specimens from a Hand, Foot, and Mouth Disease Outbreak in Singapore by Reverse Transcription-PCR with Universal Enterovirus and EV71-Specific Primers. J. Clin. Microbiol. 2002; 40(8): 2823-2827.
[15] Jiang B, Zhang J, You X, et al. Diagnosis of hand, foot, and mouth disease caused by EV71 and other enteroviruses by a one-step, single tube, duplex RT-PCR. J Med Virol. 2012; 84(11):1803-1808.
[16] Jiang B, Zhang J, You X, et al. Diagnosis of hand, foot, and mouth diseas-e caused by EV71 and other enteroviruses by a one-step, single tube, dup-lex RT-PCR. J Med Virol. 2012; 84: 1803-1808.
[17] Wang M, Jiang S, Wang Y. Recombinant VP1 protein expressed in Pichia pastoris induces protective immune responses against EV71 in mice. Biochem Biophys Res Commun. 2013; 430(1):387-393.
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Cite This Article
  • APA Style

    Junwei Liu, Jiaxing Wang, Jinfeng Wang, Li Liu, Jin Li, et al. (2018). Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM. American Journal of Clinical and Experimental Medicine, 6(4), 99-102. https://doi.org/10.11648/j.ajcem.20180604.13

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    ACS Style

    Junwei Liu; Jiaxing Wang; Jinfeng Wang; Li Liu; Jin Li, et al. Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM. Am. J. Clin. Exp. Med. 2018, 6(4), 99-102. doi: 10.11648/j.ajcem.20180604.13

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    AMA Style

    Junwei Liu, Jiaxing Wang, Jinfeng Wang, Li Liu, Jin Li, et al. Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM. Am J Clin Exp Med. 2018;6(4):99-102. doi: 10.11648/j.ajcem.20180604.13

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  • @article{10.11648/j.ajcem.20180604.13,
      author = {Junwei Liu and Jiaxing Wang and Jinfeng Wang and Li Liu and Jin Li and Haiqiu Ma},
      title = {Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {6},
      number = {4},
      pages = {99-102},
      doi = {10.11648/j.ajcem.20180604.13},
      url = {https://doi.org/10.11648/j.ajcem.20180604.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20180604.13},
      abstract = {Aim: The HFMD caused by EV71 virus has extremely high lethality and morbidity. EV71's pathogenesis has not understood thoroughly and the antiviral drug has been under development, which led to its emergence as a clinically fatal neurotropic virus. Early diagnosis is one of the main methods to reduce mortality. The aim of this study was to establish and evaluate a colloidal gold immunochromatography for qualitatively detecting the EV71-IgM in serum sample. Method: In this study, the different kinds of coating antigen were the focus of optimization. The pH value, particle size of the colloidal gold and other parameters and materials were also considered to achieve the good performance in detecting strips. The CA16-positive, EV71-positive and negative serum were used to compare and evaluate the accuracy and specificity of strips. Result: The accuracy of the strips coated by EV71-VP1-VP2-VP3 was higher than the EV71-VP1 strips 20%. The specificity of the EV71-VP1 strips was slightly higher than EV71-VP1-VP2-VP3 strips according to CA16 positive serum samples. Compared with commercial strips, this diagnostic method just needed 2 µL serum samples for detection, and the accuracy of the EV71-VP1-VP2-VP3 strips was significantly higher than the commercial strips. Conclusion: The developed method established in this study can apply to early diagnosis of HFMD caused by EV71 virus.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM
    AU  - Junwei Liu
    AU  - Jiaxing Wang
    AU  - Jinfeng Wang
    AU  - Li Liu
    AU  - Jin Li
    AU  - Haiqiu Ma
    Y1  - 2018/10/18
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ajcem.20180604.13
    DO  - 10.11648/j.ajcem.20180604.13
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 99
    EP  - 102
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20180604.13
    AB  - Aim: The HFMD caused by EV71 virus has extremely high lethality and morbidity. EV71's pathogenesis has not understood thoroughly and the antiviral drug has been under development, which led to its emergence as a clinically fatal neurotropic virus. Early diagnosis is one of the main methods to reduce mortality. The aim of this study was to establish and evaluate a colloidal gold immunochromatography for qualitatively detecting the EV71-IgM in serum sample. Method: In this study, the different kinds of coating antigen were the focus of optimization. The pH value, particle size of the colloidal gold and other parameters and materials were also considered to achieve the good performance in detecting strips. The CA16-positive, EV71-positive and negative serum were used to compare and evaluate the accuracy and specificity of strips. Result: The accuracy of the strips coated by EV71-VP1-VP2-VP3 was higher than the EV71-VP1 strips 20%. The specificity of the EV71-VP1 strips was slightly higher than EV71-VP1-VP2-VP3 strips according to CA16 positive serum samples. Compared with commercial strips, this diagnostic method just needed 2 µL serum samples for detection, and the accuracy of the EV71-VP1-VP2-VP3 strips was significantly higher than the commercial strips. Conclusion: The developed method established in this study can apply to early diagnosis of HFMD caused by EV71 virus.
    VL  - 6
    IS  - 4
    ER  - 

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Author Information
  • High-throughput Molecular Drug Discovery Center, Tianjin International Joint Academy of Biomedicine, Tianjin International Joint Academy of Biomedicine Co., Ltd, Tianjin, People’s Republic of China

  • College of Pharmacy, Nankai University, Tianjin, People’s Republic of China

  • College of Pharmacy, Nankai University, Tianjin, People’s Republic of China

  • College of Pharmacy, Nankai University, Tianjin, People’s Republic of China

  • College of Pharmacy, Nankai University, Tianjin, People’s Republic of China

  • High-throughput Molecular Drug Discovery Center, Tianjin International Joint Academy of Biomedicine, Tianjin International Joint Academy of Biomedicine Co., Ltd, Tianjin, People’s Republic of China

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