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Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes

Received: 27 March 2018     Accepted: 15 April 2018     Published: 19 May 2018
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Abstract

Clinically, Lobelia chinensis has the potential to treat Streptococcus pyogenes (GAS) infections. This study demonstrated that Lobelia chinensis and penicillin have comparative inhibitory effects when their concentration was 12 mg/mL. To uncover the possible pathways of inhibition of GAS by Lobelia chinensis, transcriptome analysis was used to explore significantly changed genes when GAS was cultured under Lobelia chinensi. Lobelia chinensis could induce alterations of 366 genes in expression level, mainly involving biosynthesis process, translation, cytoplasm, and lipid, carbohydrate metabolic process. In addition, penicillin only induced 17 genes alteration and no GO/KEGG pathway enrichment. Therefore, Lobelia chinensis showed more modes of regulating GAS than penicillin. The regulatory modes of Lobelia chinensis may be the inhibition of cell replication and growth of GAS. This study indicated that Lobelia chinens is a potential drug for the treatment of GAS infection due to its considerable inhibition effects and multiple inhibition modes.

Published in American Journal of Clinical and Experimental Medicine (Volume 6, Issue 2)
DOI 10.11648/j.ajcem.20180602.13
Page(s) 46-57
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Lobelia chinensis, Streptococcus pyogenes, Penicillin, Transcriptome

References
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[2] Carapetis, J. R., Steer, A. C., Mulholland, E. K., and Weber, M. (2005). The global burden of group A streptococcal diseases. The Lancet. Infectious diseases 5, 685-694.
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  • APA Style

    Xiaoying Lin, Xiangyu Kong, Chengping Wen, Zhixing He. (2018). Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes. American Journal of Clinical and Experimental Medicine, 6(2), 46-57. https://doi.org/10.11648/j.ajcem.20180602.13

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    ACS Style

    Xiaoying Lin; Xiangyu Kong; Chengping Wen; Zhixing He. Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes. Am. J. Clin. Exp. Med. 2018, 6(2), 46-57. doi: 10.11648/j.ajcem.20180602.13

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    AMA Style

    Xiaoying Lin, Xiangyu Kong, Chengping Wen, Zhixing He. Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes. Am J Clin Exp Med. 2018;6(2):46-57. doi: 10.11648/j.ajcem.20180602.13

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  • @article{10.11648/j.ajcem.20180602.13,
      author = {Xiaoying Lin and Xiangyu Kong and Chengping Wen and Zhixing He},
      title = {Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {6},
      number = {2},
      pages = {46-57},
      doi = {10.11648/j.ajcem.20180602.13},
      url = {https://doi.org/10.11648/j.ajcem.20180602.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20180602.13},
      abstract = {Clinically, Lobelia chinensis has the potential to treat Streptococcus pyogenes (GAS) infections. This study demonstrated that Lobelia chinensis and penicillin have comparative inhibitory effects when their concentration was 12 mg/mL. To uncover the possible pathways of inhibition of GAS by Lobelia chinensis, transcriptome analysis was used to explore significantly changed genes when GAS was cultured under Lobelia chinensi. Lobelia chinensis could induce alterations of 366 genes in expression level, mainly involving biosynthesis process, translation, cytoplasm, and lipid, carbohydrate metabolic process. In addition, penicillin only induced 17 genes alteration and no GO/KEGG pathway enrichment. Therefore, Lobelia chinensis showed more modes of regulating GAS than penicillin. The regulatory modes of Lobelia chinensis may be the inhibition of cell replication and growth of GAS. This study indicated that Lobelia chinens is a potential drug for the treatment of GAS infection due to its considerable inhibition effects and multiple inhibition modes.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes
    AU  - Xiaoying Lin
    AU  - Xiangyu Kong
    AU  - Chengping Wen
    AU  - Zhixing He
    Y1  - 2018/05/19
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ajcem.20180602.13
    DO  - 10.11648/j.ajcem.20180602.13
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 46
    EP  - 57
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20180602.13
    AB  - Clinically, Lobelia chinensis has the potential to treat Streptococcus pyogenes (GAS) infections. This study demonstrated that Lobelia chinensis and penicillin have comparative inhibitory effects when their concentration was 12 mg/mL. To uncover the possible pathways of inhibition of GAS by Lobelia chinensis, transcriptome analysis was used to explore significantly changed genes when GAS was cultured under Lobelia chinensi. Lobelia chinensis could induce alterations of 366 genes in expression level, mainly involving biosynthesis process, translation, cytoplasm, and lipid, carbohydrate metabolic process. In addition, penicillin only induced 17 genes alteration and no GO/KEGG pathway enrichment. Therefore, Lobelia chinensis showed more modes of regulating GAS than penicillin. The regulatory modes of Lobelia chinensis may be the inhibition of cell replication and growth of GAS. This study indicated that Lobelia chinens is a potential drug for the treatment of GAS infection due to its considerable inhibition effects and multiple inhibition modes.
    VL  - 6
    IS  - 2
    ER  - 

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Author Information
  • College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China

  • College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China

  • College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China

  • College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China

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