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Effect of Oral Insulin on the Development of Small Intestine Carbohydrate Hydrolysis and Absorption in Suckling Rats

Received: 28 February 2017     Accepted: 10 April 2017     Published: 16 August 2017
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Abstract

The study is conducted to reveal the effect of oral insulin on the development of carbohydrate hydrolysis and transport in the small intestine in suckling rats. Oral insulin administration (5 U/kg/day) from the 1st to the 21st day of postnatal life resulted in induction of the carbohydrate assimilation in the small intestine of growing rats. Oral insulin increased intestinal maltase, sucrase and lactase activity as well as absorption of free and hydrolysis-dependent glucose from maltose in the small intestine of suckling rats. The effect was age-dependent and was clearly expressed during the second half of the milk nutrition period. After weaning oral insulin effect on the intestinal carbohydrate digestion and absorption was absent. These data show oral insulin keeps its biological activity in the intestinal cavity and they suggest involvement of milk insulin in the hydrolysis and absorption of carbohydrates in the small intestine.

Published in American Journal of Clinical and Experimental Medicine (Volume 5, Issue 5)
DOI 10.11648/j.ajcem.20170505.11
Page(s) 157-161
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Oral Insulin, Disaccharidases, Glucose Absorption, Small Intestine, Suckling Rats

References
[1] Henning SJ. Ontogeny of enzymes in the small intestine. Annul Rev Physiol. 1985; 47: 231–245.
[2] Ugolev AM. Teoriya adekvatnogo pitaniya i trofologiya. Leningrad. Nauka, 1991. 272 p.
[3] Drozdowski LA, Clandinin N, Thomson ABR. Ontogeny, growth and development of the small intestine: Understanding pediatric gastroenterology. World J Gastroenterol. 2010; 21: 787–799.
[4] Henning SJ, Rubin RC, Shulman RJ. Ontogeny of the intestinal mucosa. In: Physiology of the Gastrointestinal Tract, LR Johnson (ed), Raven Press. 1994. 571-610.
[5] Kien CL, Heitlinger LA, Li BU, Murray RD. Digestion, absorption, and fermentation of carbohydrates. Semin Perinatol. 1989. 13(2): 78-87.
[6] Rahimov KR, Demidova AI, Mahmudov AA. Vozrastnyie osobennosti izmeneniy aktivnosti fermentov, realizuyuschih polostnoy i membrannyiy gidroliz uglevodov pri stresse. Fiziol zhurn SSSR im. I. M. Sechenova. 1996; 82 (3): 89-94.
[7] Lebenthal A, Lebenthal E. The ontogeny of the small intestinal epithelium. Journal of parenteral and enteral nutrition. 1999; 23 (50): 3–6.
[8] Persson EK, Jaensson E, Agace WW. The diverse ontogeny and function of murine small intestinal dendritic cell/macrophage subsets. Immunobiology. 2010; 215 (9-10): 692-7.
[9] Ahmetzyanova ZK, Rahimov KR. Formirovanie pischevaritelno-transportnogo konveyera v ontogeneze kryis. Zhurn evol biohim i fiziol. 2001; 37: 43-7.
[10] Puchal AA, Buddington RK. Postnatal development of monosaccharide transport in pig intestine. Am J Physiol. 1992; 262: G895–G902.
[11] Kuchkarova LS, Kudeshova GT. Effect of hydrocortisone on juvenile and definitive systems of disaccharide assimilation in the rat small intestine European Journal of Biomedical and Life Sciences. 2016; (2): 4–6.
[12] Mc Donald MC, Henning SJ. Synergistic effects of thyroxine and dexamethasone on enzyme ontogeny in rat small intestine. Pediatr Res. 1992; 32: 306–311.
[13] Yeh KY, Yeh M, Holt PR. Thyroxine and cortisone cooperate to modulate postnatal intestinal enzyme differentiation in the rat. Am J Physiol. 1991; 260: G371–8.
[14] D'Agostino J, Vaeth GF, Henning SJ. Diurnal rhythm of total and free concentrations serum corticosterone in the rat. Acta Endocrinol. 1982; 100: 85–90.
[15] Oberkotter LV. Suckling, but not formula feeding, induces a transient hyperthyroxinemia in rat pups. Endocrinology. 1988; 123(1): 127-33.
[16] Kinouchi T, Koizumi K, Kuwata T, Yajima T. Crucial role of milk-borne insulin in the development of pancreatic amylase at the onset of weaning in rats. Am J Physiol. 1998; 275: R1958-67.
[17] Kinouchi T, Koizumi K, Kuwata T, Yajima T. Milk-borne insulin with trypsin inhibitor in milk induces pancreatic amylase development at the onset of weaning in rats. J Pediatr Gastroenterol Nutr. 2000; 30 (5): 515-21.
[18] Ugolev AM, Zaripov BZ, Iezuitova NN, Gruzdkov AA, Ryibin IS, Nikitina AA, Punin MYu. Osobennosti membrannogo gidroliza i transporta v tonkoy kishke v usloviyah, blizkih k fiziologicheskim (reviziya suschestvuyuschih dannyih i predstavleniy). Biol membranyi. 1984; 1(10): 997-1018.
[19] Dahlqvist A. Assay of intestinal disaccharidases. Scand J Clin Lab Invest. 1984; 44 (2): 169–72.
[20] Lowry OH, Rosebrough NJ, Farral, Randal L RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193 (1): 265-75.
[21] Scott TA, Melvin EH. Determination of Dextran with Anthrone. Anal Chem. 1953: 25 (11): 1656–61.
[22] Kuchkarova LS. Vliyanie insulina na aktivnost kishechnyih gidrolaz u rastuschih kryis Izvestiya VUZOV. 2002; (2-4): 67-70.
[23] Malo C, Ménard D. Synergistic effects of insulin and thyroxine on the differentiation and proliferation of epithelial cells of suckling mouse small intestine. Biol Neonate. 1983; 44: 177–84.
[24] Buts JP, Duranton B, De KN, Sokal EM, Maernhout AS, Raul F. Premature stimulation of rat sucrase-isomaltase (SI) by exogenous insulin and the analog B-Asp 10 is regulated by a receptor-mediated signal triggering SI gene transcription. Pediatr Res. 1998; 43: 585–91.
[25] Kuchkarova LS. Vliyanie tiroksina na gidroliticheskuyu i transportnuyu aktivnost' tonkoj kishki v period molochnogo pitaniya. Uzb biol zhurn. 2002; (1); 10-4.
[26] Kuchkarova LS, Sadykov BA. Vliyanie hronicheskih in’ekciy gidrokortizona, tiroksina i insulina na vsasyvanie glyukozy u rastushchih krys. Pediatriya. 2004; (1-2): 53-7.
[27] Freund JN, Foltzer-Jourdainne C, Duluc I, Galluser M, Gosse F, Raul F. Rat lactase activity and mRNA expression in relation to the thyroid and corticoid status. Cell Mol Biol. 1991; 37(4): 463-6.
[28] Marandi S1, De Keyser N, Saliez A, Maernoudt AS, Sokal EM, Stilmant C, Rider MH, Buts JP. Insulin signal transduction in rat small intestine: role of MAP kinases in expression of mucosal hydrolases. Am J Physiol Gastrointest Liver Physiol. 2001; 280 (2): G229-40.
[29] Hartla WH., Alpers DH The trophic effects of substrate, insulin, and the route of administration on protein synthesis and the preservation of small bowel mucosal mass in large mammals. Clinical Nutrition. 2011; 30 (Is 1): 20–7.
[30] Shehadeh N, Sukhotnik I, Shamir R: Gastrointestinal tract as a target organ for orally administered insulin. J Pediatr Gastroenterol Nutr. 2006; 43: 276–81.
[31] Shulman RJ. Oral insulin increases small intestinal mass and disaccharidase activity in the newborn miniature pig. Pediatr Res. 1990; 28: 171–5.
[32] Shen WH, Xu RJ. Gastrointestinal stability and absorption of insulin in suckling pigs. Comp Biochem Physiol. 2000; 125 (3): 389-401.
[33] Koldovský O. Hormones in milk. In G. Litwack (ed.), Vitamins and Hormones (Chap. 2), Academic Press, New York, 1995. 50: 77–149.
[34] Slebodzinski AB, Nowak J, Gawecka H, et al. Thyroid hormones and insulin in milk: a comparative study. Endocrinology Experiment. 1986; 20: 247–55.
[35] Mac Donald RS. The role of insulin-like growth factors in small intestinal cell growth and development. Horm Metab Res. 1999; 31 (2-3): 103-13.
Cite This Article
  • APA Style

    Lubov Salijanovna Kuchkarova, Dustmatova Gulnara Abdurashidovna. (2017). Effect of Oral Insulin on the Development of Small Intestine Carbohydrate Hydrolysis and Absorption in Suckling Rats. American Journal of Clinical and Experimental Medicine, 5(5), 157-161. https://doi.org/10.11648/j.ajcem.20170505.11

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    ACS Style

    Lubov Salijanovna Kuchkarova; Dustmatova Gulnara Abdurashidovna. Effect of Oral Insulin on the Development of Small Intestine Carbohydrate Hydrolysis and Absorption in Suckling Rats. Am. J. Clin. Exp. Med. 2017, 5(5), 157-161. doi: 10.11648/j.ajcem.20170505.11

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    AMA Style

    Lubov Salijanovna Kuchkarova, Dustmatova Gulnara Abdurashidovna. Effect of Oral Insulin on the Development of Small Intestine Carbohydrate Hydrolysis and Absorption in Suckling Rats. Am J Clin Exp Med. 2017;5(5):157-161. doi: 10.11648/j.ajcem.20170505.11

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  • @article{10.11648/j.ajcem.20170505.11,
      author = {Lubov Salijanovna Kuchkarova and Dustmatova Gulnara Abdurashidovna},
      title = {Effect of Oral Insulin on the Development of Small Intestine Carbohydrate Hydrolysis and Absorption in Suckling Rats},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {5},
      number = {5},
      pages = {157-161},
      doi = {10.11648/j.ajcem.20170505.11},
      url = {https://doi.org/10.11648/j.ajcem.20170505.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170505.11},
      abstract = {The study is conducted to reveal the effect of oral insulin on the development of carbohydrate hydrolysis and transport in the small intestine in suckling rats. Oral insulin administration (5 U/kg/day) from the 1st to the 21st day of postnatal life resulted in induction of the carbohydrate assimilation in the small intestine of growing rats. Oral insulin increased intestinal maltase, sucrase and lactase activity as well as absorption of free and hydrolysis-dependent glucose from maltose in the small intestine of suckling rats. The effect was age-dependent and was clearly expressed during the second half of the milk nutrition period. After weaning oral insulin effect on the intestinal carbohydrate digestion and absorption was absent. These data show oral insulin keeps its biological activity in the intestinal cavity and they suggest involvement of milk insulin in the hydrolysis and absorption of carbohydrates in the small intestine.},
     year = {2017}
    }
    

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    T1  - Effect of Oral Insulin on the Development of Small Intestine Carbohydrate Hydrolysis and Absorption in Suckling Rats
    AU  - Lubov Salijanovna Kuchkarova
    AU  - Dustmatova Gulnara Abdurashidovna
    Y1  - 2017/08/16
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    DO  - 10.11648/j.ajcem.20170505.11
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
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    EP  - 161
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20170505.11
    AB  - The study is conducted to reveal the effect of oral insulin on the development of carbohydrate hydrolysis and transport in the small intestine in suckling rats. Oral insulin administration (5 U/kg/day) from the 1st to the 21st day of postnatal life resulted in induction of the carbohydrate assimilation in the small intestine of growing rats. Oral insulin increased intestinal maltase, sucrase and lactase activity as well as absorption of free and hydrolysis-dependent glucose from maltose in the small intestine of suckling rats. The effect was age-dependent and was clearly expressed during the second half of the milk nutrition period. After weaning oral insulin effect on the intestinal carbohydrate digestion and absorption was absent. These data show oral insulin keeps its biological activity in the intestinal cavity and they suggest involvement of milk insulin in the hydrolysis and absorption of carbohydrates in the small intestine.
    VL  - 5
    IS  - 5
    ER  - 

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Author Information
  • Department of Physiology and Neurobiology, National University of Uzbekistan, Tashkent, Uzbekistan

  • Department of Physiology and Neurobiology, National University of Uzbekistan, Tashkent, Uzbekistan

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