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Interaction of Titanium Dioxide Nanoparticles and Blood Serum of Patients with Bronchial Asthma and Myasthenia Gravis

Received: 6 May 2015     Accepted: 13 May 2015     Published: 26 May 2015
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Abstract

The interest to nanoobjects is mainly attracted by the fact that their properties (and also biological in particular interest to the project) differ drastically from the properties of either bulk material or constituent molecules. The fundamental task is correlation between structure of the nanoparticles and their interaction with surrounding media. The aim of project is a study of interaction of titanium dioxide nanoparticles and blood serum of patients with verified myasthenia and allergy. Laser correlation spectroscopy (LCS) allows fast and easy hydrodynamic diameter determination in a broad range from nanometer to hundred micrometers, which is an advantage in comparison to other technique for a mixture of nano and microobjects of very different sizes, which constitutes biological liquids. LCS measurement of titanium dioxide nanoparticles specimen showed that main part of light scattering is due to particles with radial size 4.64-8.42 nm. LCS and other physical methods measurement data are coincided. Comparison of LC-histograms of blood serum revealed increased contribution of 4.64 -15.28 nm particles into light scattering in bronchial asthma patients and 404.65 – 1794.35 nm particles in myasthenia gravis patients. Interaction between titanium dioxide nanoparticles, stabilized by phosphoric acid, and blood serum results to albumin- and globulin-containing complexes formation and precipitation. Interaction between titanium dioxide nanoparticles, stabilized by phosphoric acid, and blood serum results to albumin- and globulin-containing complexes formation and precipitation. Interaction between titanium dioxide nanoparticles and myasthenia patients blood serum results to removing of considerable part (40%) of circulating immune complexes. TiO2 nanoparticles under interaction with asthma patients blood serum form complexes with small size proteins. LCS analysis confirmed removal of particles corresponding by their size to the major serum proteins and redistribution towards larger particles presented by glycolipids, glycoproteins, and antigen-antibody complexes.

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 3)
DOI 10.11648/j.ajcem.20150303.20
Page(s) 128-132
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Autoimmune Complexes, Bronchial Asthma, Laser Correlation Spectroscopy, Myasthenia Gravis, Nanoparticles, Titanium Dioxide

References
[1] W.H.De Jong, P.JA Borm, "Drug delivery and nanoparticles: Applications and hazards," International Journal of Nanomedicine, 2008, 3(2), pp.133-149.
[2] P. Thevenot, Jai Cho, D. Wavhal, R. B. Timmons, L. Tang, "Surface chemistry influences cancer killing effect of TiO2 nanoparticles," Nanomedicine: NBM, 2008, 4:226-236. doi:10.1016/j.nano.2008.04.001.
[3] Bae Y. H. and Park K., "Targeted drug delivery to tumors: Myths, reality and possibility," J. Controlled Release, 2012; 153(3), pp. 198–205.
[4] Ezhova O., Klebnikova N., Alchinova I., Arkhipova E., Torshin V., Karganov M., "New methods of laboratory diagnosis and monitoring the effectiveness of allergic diseases," Vestnik RUDN. Ser. Medicina, 2008, 3:52-58. (in Russian)
[5] Karganov M., Kiselyov M., Komarov G., Kuinzhi N., Kurneshova L., Kuchma V., etc. "Polysystem sanogenetichesky monitoring," Moscow, MIPKRO, 2001, 344 p. (in Russian)
[6] Titov V.N., Karganov M.Yu., Rotenko A.A., Dmitriyev V.A., Alchinova I.B., Arkhipova E.N., "Biological functions and biological reactions. laser correlation spectroscopy in an assessment of purity of the intercellular environment - functions of an endoekologiya (lecture)," Klinicheskaja laboratornaja diagnostika, 2009, vol. 6, pp. 21-36. (in Russian)
[7] Karganov M., Alchinova I., Arkhipova E., Skalny A.V., "Laser Correlation Spectroscopy: Nutritional, Ecological and Toxic Aspects," In: “Biophysics”. A.N. Misra ed.- InTech. 2012, ISBN 978-953-51-0376-9: pp.1-16.
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[9] Alchinova I., Arkhipova E., Cherepov A., Karganov M., "Polysystemic monitoring of nuclear plant stuff and risk group revealing," In: Risk assessment and management. Zhang Zhiyong ed., 2012, Academy Publish, USA. ISBN: 978-0-9835850-2-2. P. 193-199.
[10] Mirshafiee V., Mahmoudi M., Lou K., Cheng J. and Kraft M. L., "Protein corona significantly reduces active targeting yield," Chem. Commun, 2013, vol. 49, pp. 2557–9. doi: 10.1039/c3cc37307j.
[11] Zhdanov R.I., Strazhevskaja N.B., Shmyrina A.S., Krylov A.S., Zarubina T.V., Karganov M.Yu. et al., "Instability of genomic DNA and change of composition of fatty acids of the DNA-connected lipids of a spleen of rats at the folat-induced gipergomotsisteinemiya," Patogenez. 2006, 4 (4), pp. 82-92. (in Russian)
[12] Piruzjan L.A., Kovalev I.E., Kovaleva V.L., Tjumenceva E.S., Balabolkin I.I., Karganov M.Yu. et al., "Laser correlation spectroscopy of macromolecular complexes in blood serum as an effective method of an assessment of a course of a disease of bronchial asthma at children," Doklady Akademii nauk, 2004, 395(6), pp. 832-836. (in Russian)
[13] Alchinova, E. Arkhipova, D. Sidnev, A. Sanadze, S. Dedaev, and M. Karganov, "Comparative Analysis of the Informative Value of Radioimmunoassay and Laser Correlation Spectroscopy in Myasthenia Gravis, " ISRN Immunology, vol. 2014, Article ID 718393, 5 pages, 2014.doi:10.1155/2014/718393
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    Elena Arkhipova, Irina Alchinova, Alexander Sanadze, Leonid Goldenberg, Mikhail Karganov. (2015). Interaction of Titanium Dioxide Nanoparticles and Blood Serum of Patients with Bronchial Asthma and Myasthenia Gravis. American Journal of Clinical and Experimental Medicine, 3(3), 128-132. https://doi.org/10.11648/j.ajcem.20150303.20

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    ACS Style

    Elena Arkhipova; Irina Alchinova; Alexander Sanadze; Leonid Goldenberg; Mikhail Karganov. Interaction of Titanium Dioxide Nanoparticles and Blood Serum of Patients with Bronchial Asthma and Myasthenia Gravis. Am. J. Clin. Exp. Med. 2015, 3(3), 128-132. doi: 10.11648/j.ajcem.20150303.20

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    AMA Style

    Elena Arkhipova, Irina Alchinova, Alexander Sanadze, Leonid Goldenberg, Mikhail Karganov. Interaction of Titanium Dioxide Nanoparticles and Blood Serum of Patients with Bronchial Asthma and Myasthenia Gravis. Am J Clin Exp Med. 2015;3(3):128-132. doi: 10.11648/j.ajcem.20150303.20

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  • @article{10.11648/j.ajcem.20150303.20,
      author = {Elena Arkhipova and Irina Alchinova and Alexander Sanadze and Leonid Goldenberg and Mikhail Karganov},
      title = {Interaction of Titanium Dioxide Nanoparticles and Blood Serum of Patients with Bronchial Asthma and Myasthenia Gravis},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {3},
      number = {3},
      pages = {128-132},
      doi = {10.11648/j.ajcem.20150303.20},
      url = {https://doi.org/10.11648/j.ajcem.20150303.20},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150303.20},
      abstract = {The interest to nanoobjects is mainly attracted by the fact that their properties (and also biological in particular interest to the project) differ drastically from the properties of either bulk material or constituent molecules. The fundamental task is correlation between structure of the nanoparticles and their interaction with surrounding media. The aim of project is a study of interaction of titanium dioxide nanoparticles and blood serum of patients with verified myasthenia and allergy. Laser correlation spectroscopy (LCS) allows fast and easy hydrodynamic diameter determination in a broad range from nanometer to hundred micrometers, which is an advantage in comparison to other technique for a mixture of nano and microobjects of very different sizes, which constitutes biological liquids. LCS measurement of titanium dioxide nanoparticles specimen showed that main part of light scattering is due to particles with radial size 4.64-8.42 nm. LCS and other physical methods measurement data are coincided. Comparison of LC-histograms of blood serum revealed increased contribution of 4.64 -15.28 nm particles into light scattering in bronchial asthma patients and 404.65 – 1794.35 nm particles in myasthenia gravis patients. Interaction between titanium dioxide nanoparticles, stabilized by phosphoric acid, and blood serum results to albumin- and globulin-containing complexes formation and precipitation. Interaction between titanium dioxide nanoparticles, stabilized by phosphoric acid, and blood serum results to albumin- and globulin-containing complexes formation and precipitation. Interaction between titanium dioxide nanoparticles and myasthenia patients blood serum results to removing of considerable part (40%) of circulating immune complexes. TiO2 nanoparticles under interaction with asthma patients blood serum form complexes with small size proteins. LCS analysis confirmed removal of particles corresponding by their size to the major serum proteins and redistribution towards larger particles presented by glycolipids, glycoproteins, and antigen-antibody complexes.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Interaction of Titanium Dioxide Nanoparticles and Blood Serum of Patients with Bronchial Asthma and Myasthenia Gravis
    AU  - Elena Arkhipova
    AU  - Irina Alchinova
    AU  - Alexander Sanadze
    AU  - Leonid Goldenberg
    AU  - Mikhail Karganov
    Y1  - 2015/05/26
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajcem.20150303.20
    DO  - 10.11648/j.ajcem.20150303.20
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 128
    EP  - 132
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20150303.20
    AB  - The interest to nanoobjects is mainly attracted by the fact that their properties (and also biological in particular interest to the project) differ drastically from the properties of either bulk material or constituent molecules. The fundamental task is correlation between structure of the nanoparticles and their interaction with surrounding media. The aim of project is a study of interaction of titanium dioxide nanoparticles and blood serum of patients with verified myasthenia and allergy. Laser correlation spectroscopy (LCS) allows fast and easy hydrodynamic diameter determination in a broad range from nanometer to hundred micrometers, which is an advantage in comparison to other technique for a mixture of nano and microobjects of very different sizes, which constitutes biological liquids. LCS measurement of titanium dioxide nanoparticles specimen showed that main part of light scattering is due to particles with radial size 4.64-8.42 nm. LCS and other physical methods measurement data are coincided. Comparison of LC-histograms of blood serum revealed increased contribution of 4.64 -15.28 nm particles into light scattering in bronchial asthma patients and 404.65 – 1794.35 nm particles in myasthenia gravis patients. Interaction between titanium dioxide nanoparticles, stabilized by phosphoric acid, and blood serum results to albumin- and globulin-containing complexes formation and precipitation. Interaction between titanium dioxide nanoparticles, stabilized by phosphoric acid, and blood serum results to albumin- and globulin-containing complexes formation and precipitation. Interaction between titanium dioxide nanoparticles and myasthenia patients blood serum results to removing of considerable part (40%) of circulating immune complexes. TiO2 nanoparticles under interaction with asthma patients blood serum form complexes with small size proteins. LCS analysis confirmed removal of particles corresponding by their size to the major serum proteins and redistribution towards larger particles presented by glycolipids, glycoproteins, and antigen-antibody complexes.
    VL  - 3
    IS  - 3
    ER  - 

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Author Information
  • Institute of General Pathology and Pathophysiology of Russian Academy of Medical Sciences, Laboratory of Physicochemical and Ecological Pathophysiology, Moscow, Russia

  • Institute of General Pathology and Pathophysiology of Russian Academy of Medical Sciences, Laboratory of Physicochemical and Ecological Pathophysiology, Moscow, Russia

  • SFHI Municipal Clinical Hospital No. 51, Moscow City Health Department, Moscow Myasthenia Center, Moscow, Russia

  • Plasmachem GmbH, Berlin, Germany

  • Institute of General Pathology and Pathophysiology of Russian Academy of Medical Sciences, Laboratory of Physicochemical and Ecological Pathophysiology, Moscow, Russia

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