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Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi

Received: 29 March 2015     Accepted: 12 April 2015     Published: 19 May 2015
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Abstract

Background: Lippia adoensis var. koseret is a well known medicinal plant endemic to Ethiopia. It has been traditionally used to treat different infectious diseases and also in food preparation as condiment. The aim of the current study was to evaluate antibacterial and antifungal activities of water, ethanol and methanol based crude extracts of L. adoensis var. koseret against selected human pathogenic bacteria and fungi. Methods: Crude extracts of L. adoensis var. koseret were extracted by maceration method. Disc diffusion assay of the extracts were carried out in four different concentrations against three different bacteria species (Staphylococcus aureus, Enterococcus faecalis and Escherichia coli) and two clinical isolated fungal species (Candida albicans and Aspergillus flavus) by using Kirby- Baur disk diffusion method. Agar dilution method was used to determine the minimum inhibitory concentration, the minimum bactericidal and fungicidal concentrations of the extracts against similar microorganisms. Results: Water-based extract of L. adoensis var. koseret exhibited significantly less antimicrobial activity as compared to ethanol and methanol crude extract against tested isolates of bacteria and fungi (P < 0.05); while, there was no significant difference between ethanol and methanol extracts. Among the tested microorganism S. aureus, was the most sensitive of all whereas C. albicans was the most resistant microorganism to alcohol based extract of L. adoensis var. koseret. The minimum inhibitory concentration of L. adoensis var. koseret ranged from 3.12 to 25mg/ml in the alcohol based extracts but it was higher in the water-based extract. The lower bactericidal concentration (5.20 mg/l) and the highest fungicidal concentration (37.50 mg/ml) were observed in methanol based extracts against S. aureus and C. albicans, respectively. Conclusions: Antimicrobial activity of L. adoensis var. koseret varies with extraction solvent and tested microorganisms.

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 3)
DOI 10.11648/j.ajcem.20150303.18
Page(s) 118-123
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Antibacterial Activity, Antifungal Activity, Crude Extract, L. adoensis var. koseret

References
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    Gemechu Ameya Buli, Abdella Gure Duga, Engda Dessalegn. (2015). Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi. American Journal of Clinical and Experimental Medicine, 3(3), 118-123. https://doi.org/10.11648/j.ajcem.20150303.18

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    ACS Style

    Gemechu Ameya Buli; Abdella Gure Duga; Engda Dessalegn. Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi. Am. J. Clin. Exp. Med. 2015, 3(3), 118-123. doi: 10.11648/j.ajcem.20150303.18

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    AMA Style

    Gemechu Ameya Buli, Abdella Gure Duga, Engda Dessalegn. Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi. Am J Clin Exp Med. 2015;3(3):118-123. doi: 10.11648/j.ajcem.20150303.18

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  • @article{10.11648/j.ajcem.20150303.18,
      author = {Gemechu Ameya Buli and Abdella Gure Duga and Engda Dessalegn},
      title = {Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {3},
      number = {3},
      pages = {118-123},
      doi = {10.11648/j.ajcem.20150303.18},
      url = {https://doi.org/10.11648/j.ajcem.20150303.18},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150303.18},
      abstract = {Background: Lippia adoensis var. koseret is a well known medicinal plant endemic to Ethiopia. It has been traditionally used to treat different infectious diseases and also in food preparation as condiment. The aim of the current study was to evaluate antibacterial and antifungal activities of water, ethanol and methanol based crude extracts of L. adoensis var. koseret against selected human pathogenic bacteria and fungi. Methods: Crude extracts of L. adoensis var. koseret were extracted by maceration method. Disc diffusion assay of the extracts were carried out in four different concentrations against three different bacteria species (Staphylococcus aureus, Enterococcus faecalis and Escherichia coli) and two clinical isolated fungal species (Candida albicans and Aspergillus flavus) by using Kirby- Baur disk diffusion method. Agar dilution method was used to determine the minimum inhibitory concentration, the minimum bactericidal and fungicidal concentrations of the extracts against similar microorganisms. Results: Water-based extract of L. adoensis var. koseret exhibited significantly less antimicrobial activity as compared to ethanol and methanol crude extract against tested isolates of bacteria and fungi (P < 0.05); while, there was no significant difference between ethanol and methanol extracts. Among the tested microorganism S. aureus, was the most sensitive of all whereas C. albicans was the most resistant microorganism to alcohol based extract of L. adoensis var. koseret. The minimum inhibitory concentration of L. adoensis var. koseret ranged from 3.12 to 25mg/ml in the alcohol based extracts but it was higher in the water-based extract. The lower bactericidal concentration (5.20 mg/l) and the highest fungicidal concentration (37.50 mg/ml) were observed in methanol based extracts against S. aureus and C. albicans, respectively. Conclusions: Antimicrobial activity of L. adoensis var. koseret varies with extraction solvent and tested microorganisms.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi
    AU  - Gemechu Ameya Buli
    AU  - Abdella Gure Duga
    AU  - Engda Dessalegn
    Y1  - 2015/05/19
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajcem.20150303.18
    DO  - 10.11648/j.ajcem.20150303.18
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 118
    EP  - 123
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20150303.18
    AB  - Background: Lippia adoensis var. koseret is a well known medicinal plant endemic to Ethiopia. It has been traditionally used to treat different infectious diseases and also in food preparation as condiment. The aim of the current study was to evaluate antibacterial and antifungal activities of water, ethanol and methanol based crude extracts of L. adoensis var. koseret against selected human pathogenic bacteria and fungi. Methods: Crude extracts of L. adoensis var. koseret were extracted by maceration method. Disc diffusion assay of the extracts were carried out in four different concentrations against three different bacteria species (Staphylococcus aureus, Enterococcus faecalis and Escherichia coli) and two clinical isolated fungal species (Candida albicans and Aspergillus flavus) by using Kirby- Baur disk diffusion method. Agar dilution method was used to determine the minimum inhibitory concentration, the minimum bactericidal and fungicidal concentrations of the extracts against similar microorganisms. Results: Water-based extract of L. adoensis var. koseret exhibited significantly less antimicrobial activity as compared to ethanol and methanol crude extract against tested isolates of bacteria and fungi (P < 0.05); while, there was no significant difference between ethanol and methanol extracts. Among the tested microorganism S. aureus, was the most sensitive of all whereas C. albicans was the most resistant microorganism to alcohol based extract of L. adoensis var. koseret. The minimum inhibitory concentration of L. adoensis var. koseret ranged from 3.12 to 25mg/ml in the alcohol based extracts but it was higher in the water-based extract. The lower bactericidal concentration (5.20 mg/l) and the highest fungicidal concentration (37.50 mg/ml) were observed in methanol based extracts against S. aureus and C. albicans, respectively. Conclusions: Antimicrobial activity of L. adoensis var. koseret varies with extraction solvent and tested microorganisms.
    VL  - 3
    IS  - 3
    ER  - 

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Author Information
  • Department of Medical Laboratory Science, College of Medicine and Health Sciences, Arba Minch University, Arba Minch, Ethiopia

  • Wondo Genet Colleges of Forestry and Natural Resources, Hawassa University, Hawassa, Ethiopia

  • Department of Chemistry, Hawassa College of Education, Hawassa, Ethiopia

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