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Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect

Received: 3 September 2014     Accepted: 19 September 2014     Published: 30 September 2014
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Abstract

Background: Fungal infections are significant risk factors for nosocomial infections. They are associated with environmental spores and they are potential colonizers in hospital infrastructure, instruments or specific vectors. Usually they are identified by means of microbiology and culture media for definitive diagnosis. The objective is to evaluate the usefulness of the application of a colorimetric assay that originates from an endemic insect in Mexico (Dactilopius Coccus costa); It implies a specific qualitative biochemical reaction. It is also available to be used as a quick field test in health control. Design: Prospective, transversal, descriptive, randomized sampling with control reference test. Methods: A transversal randomized sampling from surfaces, materials, solutions and organic-sanitary waste from different known risk areas in a hospital institution with a large number of patients. Samples were processed using the qualitative test, examined by colorimetric evaluations and compared with positive controls (zymosan and aspergillus spores). Results: Samples showed no evidence of fungal colonization, unlike controls, which resulted positive. The resulting sensitivity was 100%. Conclusions: First qualitative pilot test to be used in the health care field, which proved to be useful for the monitoring and timely detection of fungi of biomedical interest. The method is practical. This essay validates the potential use of a quick qualitative test for preventive control of fungal infections in hospitals.

Published in American Journal of Clinical and Experimental Medicine (Volume 2, Issue 5)
DOI 10.11648/j.ajcem.20140205.12
Page(s) 97-102
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Carminic Acid, Dactylopius, Hemolymph, Intrahospital Infection, Fungal Infection Diagnosis

References
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Cite This Article
  • APA Style

    Fernando Garía-Gil De Muñoz, Ignacio Del Río-Dueñas, Rodrigo Ramos-Zúñiga, Fidel Hernández-Hernández, H. Raúl. Pérez-Gómez, et al. (2014). Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect. American Journal of Clinical and Experimental Medicine, 2(5), 97-102. https://doi.org/10.11648/j.ajcem.20140205.12

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    ACS Style

    Fernando Garía-Gil De Muñoz; Ignacio Del Río-Dueñas; Rodrigo Ramos-Zúñiga; Fidel Hernández-Hernández; H. Raúl. Pérez-Gómez, et al. Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect. Am. J. Clin. Exp. Med. 2014, 2(5), 97-102. doi: 10.11648/j.ajcem.20140205.12

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    AMA Style

    Fernando Garía-Gil De Muñoz, Ignacio Del Río-Dueñas, Rodrigo Ramos-Zúñiga, Fidel Hernández-Hernández, H. Raúl. Pérez-Gómez, et al. Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect. Am J Clin Exp Med. 2014;2(5):97-102. doi: 10.11648/j.ajcem.20140205.12

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  • @article{10.11648/j.ajcem.20140205.12,
      author = {Fernando Garía-Gil De Muñoz and Ignacio Del Río-Dueñas and Rodrigo Ramos-Zúñiga and Fidel Hernández-Hernández and H. Raúl. Pérez-Gómez and Ana Macías-Ornelas and Ramiro López-Elizalde},
      title = {Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {2},
      number = {5},
      pages = {97-102},
      doi = {10.11648/j.ajcem.20140205.12},
      url = {https://doi.org/10.11648/j.ajcem.20140205.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140205.12},
      abstract = {Background: Fungal infections are significant risk factors for nosocomial infections. They are associated with environmental spores and they are potential colonizers in hospital infrastructure, instruments or specific vectors. Usually they are identified by means of microbiology and culture media for definitive diagnosis. The objective is to evaluate the usefulness of the application of a colorimetric assay that originates from an endemic insect in Mexico (Dactilopius Coccus costa); It implies a specific qualitative biochemical reaction. It is also available to be used as a quick field test in health control. Design: Prospective, transversal, descriptive, randomized sampling with control reference test. Methods: A transversal randomized sampling from surfaces, materials, solutions and organic-sanitary waste from different known risk areas in a hospital institution with a large number of patients. Samples were processed using the qualitative test, examined by colorimetric evaluations and compared with positive controls (zymosan and aspergillus spores). Results: Samples showed no evidence of fungal colonization, unlike controls, which resulted positive. The resulting sensitivity was 100%. Conclusions: First qualitative pilot test to be used in the health care field, which proved to be useful for the monitoring and timely detection of fungi of biomedical interest. The method is practical. This essay validates the potential use of a quick qualitative test for preventive control of fungal infections in hospitals.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect
    AU  - Fernando Garía-Gil De Muñoz
    AU  - Ignacio Del Río-Dueñas
    AU  - Rodrigo Ramos-Zúñiga
    AU  - Fidel Hernández-Hernández
    AU  - H. Raúl. Pérez-Gómez
    AU  - Ana Macías-Ornelas
    AU  - Ramiro López-Elizalde
    Y1  - 2014/09/30
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajcem.20140205.12
    DO  - 10.11648/j.ajcem.20140205.12
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 97
    EP  - 102
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20140205.12
    AB  - Background: Fungal infections are significant risk factors for nosocomial infections. They are associated with environmental spores and they are potential colonizers in hospital infrastructure, instruments or specific vectors. Usually they are identified by means of microbiology and culture media for definitive diagnosis. The objective is to evaluate the usefulness of the application of a colorimetric assay that originates from an endemic insect in Mexico (Dactilopius Coccus costa); It implies a specific qualitative biochemical reaction. It is also available to be used as a quick field test in health control. Design: Prospective, transversal, descriptive, randomized sampling with control reference test. Methods: A transversal randomized sampling from surfaces, materials, solutions and organic-sanitary waste from different known risk areas in a hospital institution with a large number of patients. Samples were processed using the qualitative test, examined by colorimetric evaluations and compared with positive controls (zymosan and aspergillus spores). Results: Samples showed no evidence of fungal colonization, unlike controls, which resulted positive. The resulting sensitivity was 100%. Conclusions: First qualitative pilot test to be used in the health care field, which proved to be useful for the monitoring and timely detection of fungi of biomedical interest. The method is practical. This essay validates the potential use of a quick qualitative test for preventive control of fungal infections in hospitals.
    VL  - 2
    IS  - 5
    ER  - 

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Author Information
  • Laboratory of Molecular Entomology, Department of Molecular Pathogenesis of CINESTAV-IPN, IPN 2508, Mexico, D. F

  • Infectious Pathology Institute, Hospital Civil De Guadalajara, Guadalajara, Mexico

  • Neuroscience Department, CUCS, University of Guadalajara, Guadalajara, Mexico

  • Hospital Civil J. I. Menchaca, Guadalajara, Mexico

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