The increased usage of synthetic antibiotics for treatment and growth promotion in livestock has significantly resulted into antimicrobial resistance among livestock and human. To combat this challenge, the current study evaluated the effects of propolis, as a natural growth promoter, compared to a commercial synthetic booster on hematological and serum biochemical parameters of Cobb 500 broiler chickens. A total of 100 day-old chicks were randomly assigned to five treatment groups in a completely randomized design (CRD). The control group (CTR) received plain water, while the positive control group (CBB) received water supplemented with a commercial broiler booster. Three experimental groups (P1, P2, and P3) received propolis extract prepared from 100g, 200g, and 300g of raw propolis per liter of water, respectively. Birds were reared for 42 days, and blood samples were collected at days 3 and 42 for analysis of hematological and biochemical indices. The results showed that white blood cell indices responded variably to the type and dose of growth promoter. At day 42, the P1 group exhibited significantly elevated lymphocyte counts, suggesting enhanced humoral immunity. In contrast, the BST group showed increased neutrophil and monocyte levels, indicating possible innate immune activation or stress. Red blood cell parameters remained largely unchanged at day 3 but showed significant variations at day 42, particularly in MCV, MCHC, and RDW metrics, suggesting dose-related impacts on erythrocyte morphology. No significant differences were observed in total protein and cholesterol levels across all treatments, indicating minimal effects on liver function and lipid metabolism. Overall, the study suggests that moderate doses of propolis can enhance immune responses in broilers without adversely affecting blood biochemistry, supporting its use as a natural alternative to synthetic growth promoters.
| Published in | American Journal of Biomedical and Life Sciences (Volume 13, Issue 6) |
| DOI | 10.11648/j.ajbls.20251306.11 |
| Page(s) | 114-121 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2025. Published by Science Publishing Group |
Propolis, Broiler Chickens, Hematology, Serum biochemistry, Immune Response, Growth Promoter
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APA Style
Azza, G. Z., Chenyambuga, S. W., Lyimo, C. M. (2025). Effect of Aqueous Propolis Supplementation on Performance in Hematological and Serum Biochemical Parameters of Cobb 500 Broiler Chicken. American Journal of Biomedical and Life Sciences, 13(6), 114-121. https://doi.org/10.11648/j.ajbls.20251306.11
ACS Style
Azza, G. Z.; Chenyambuga, S. W.; Lyimo, C. M. Effect of Aqueous Propolis Supplementation on Performance in Hematological and Serum Biochemical Parameters of Cobb 500 Broiler Chicken. Am. J. Biomed. Life Sci. 2025, 13(6), 114-121. doi: 10.11648/j.ajbls.20251306.11
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Download@article{10.11648/j.ajbls.20251306.11,
author = {Glory Zerah Azza and Sebastian Wilson Chenyambuga and Charles Moses Lyimo},
title = {Effect of Aqueous Propolis Supplementation on Performance in Hematological and Serum Biochemical Parameters of Cobb 500 Broiler Chicken},
journal = {American Journal of Biomedical and Life Sciences},
volume = {13},
number = {6},
pages = {114-121},
doi = {10.11648/j.ajbls.20251306.11},
url = {https://doi.org/10.11648/j.ajbls.20251306.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20251306.11},
abstract = {The increased usage of synthetic antibiotics for treatment and growth promotion in livestock has significantly resulted into antimicrobial resistance among livestock and human. To combat this challenge, the current study evaluated the effects of propolis, as a natural growth promoter, compared to a commercial synthetic booster on hematological and serum biochemical parameters of Cobb 500 broiler chickens. A total of 100 day-old chicks were randomly assigned to five treatment groups in a completely randomized design (CRD). The control group (CTR) received plain water, while the positive control group (CBB) received water supplemented with a commercial broiler booster. Three experimental groups (P1, P2, and P3) received propolis extract prepared from 100g, 200g, and 300g of raw propolis per liter of water, respectively. Birds were reared for 42 days, and blood samples were collected at days 3 and 42 for analysis of hematological and biochemical indices. The results showed that white blood cell indices responded variably to the type and dose of growth promoter. At day 42, the P1 group exhibited significantly elevated lymphocyte counts, suggesting enhanced humoral immunity. In contrast, the BST group showed increased neutrophil and monocyte levels, indicating possible innate immune activation or stress. Red blood cell parameters remained largely unchanged at day 3 but showed significant variations at day 42, particularly in MCV, MCHC, and RDW metrics, suggesting dose-related impacts on erythrocyte morphology. No significant differences were observed in total protein and cholesterol levels across all treatments, indicating minimal effects on liver function and lipid metabolism. Overall, the study suggests that moderate doses of propolis can enhance immune responses in broilers without adversely affecting blood biochemistry, supporting its use as a natural alternative to synthetic growth promoters.},
year = {2025}
}
TY - JOUR T1 - Effect of Aqueous Propolis Supplementation on Performance in Hematological and Serum Biochemical Parameters of Cobb 500 Broiler Chicken AU - Glory Zerah Azza AU - Sebastian Wilson Chenyambuga AU - Charles Moses Lyimo Y1 - 2025/12/09 PY - 2025 N1 - https://doi.org/10.11648/j.ajbls.20251306.11 DO - 10.11648/j.ajbls.20251306.11 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 114 EP - 121 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20251306.11 AB - The increased usage of synthetic antibiotics for treatment and growth promotion in livestock has significantly resulted into antimicrobial resistance among livestock and human. To combat this challenge, the current study evaluated the effects of propolis, as a natural growth promoter, compared to a commercial synthetic booster on hematological and serum biochemical parameters of Cobb 500 broiler chickens. A total of 100 day-old chicks were randomly assigned to five treatment groups in a completely randomized design (CRD). The control group (CTR) received plain water, while the positive control group (CBB) received water supplemented with a commercial broiler booster. Three experimental groups (P1, P2, and P3) received propolis extract prepared from 100g, 200g, and 300g of raw propolis per liter of water, respectively. Birds were reared for 42 days, and blood samples were collected at days 3 and 42 for analysis of hematological and biochemical indices. The results showed that white blood cell indices responded variably to the type and dose of growth promoter. At day 42, the P1 group exhibited significantly elevated lymphocyte counts, suggesting enhanced humoral immunity. In contrast, the BST group showed increased neutrophil and monocyte levels, indicating possible innate immune activation or stress. Red blood cell parameters remained largely unchanged at day 3 but showed significant variations at day 42, particularly in MCV, MCHC, and RDW metrics, suggesting dose-related impacts on erythrocyte morphology. No significant differences were observed in total protein and cholesterol levels across all treatments, indicating minimal effects on liver function and lipid metabolism. Overall, the study suggests that moderate doses of propolis can enhance immune responses in broilers without adversely affecting blood biochemistry, supporting its use as a natural alternative to synthetic growth promoters. VL - 13 IS - 6 ER -
Department of Animal, Aquaculture and Range sciences (DAARS), Sokoine University of Agriculture (SUA), Morogoro, Tanzania
Department of Animal, Aquaculture and Range sciences (DAARS), Sokoine University of Agriculture (SUA), Morogoro, Tanzania
Department of Animal, Aquaculture and Range sciences (DAARS), Sokoine University of Agriculture (SUA), Morogoro, Tanzania
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