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Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation

Received: 9 April 2022     Accepted: 5 May 2022     Published: 24 May 2022
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Abstract

In current scientific article, rat liver mitochondria respond to the flavonoids dihydroquercetin (20–100 µM), 1-(2´-bromo-4´,5´-dimethoxyphenyl)-6,7-dimethoxy-1,2,3,4 for respiratory and processes of oxidative phosphorylation - tetrahydroisoquinolines F-18 (20-100 μM) and synthesized on the basis of followings 2-(3,4-dihydroxyphenyl)-6-[1-(2'-bromo-4',5'-dimethoxyphenyl)-6,7- The effect of dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl]methyl-3,5,7-trihydroxychroman-4-on the concentrations of the DHQ-11 conjugate (20-100 μM) was investigated in in vitro experiments. Experiments were conducted within 180-200 gram infertile white male rats. Mitochondrias from rat liver were isolated by the way of differential centrifugation. Concentrations of dihydroquercetin flavonoid 20, 60, 100 μM in FAD-dependent succinate oxidation in rat liver mitochondria were identified to be reliable respiratory control parameters and partially increased ADF/O value, the respiratory control coefficient as Chans demonstrated an increase in respiratory rate under the influence of the alkaloid F-18 isoquinoline 20, 60, 100 μM. Moreover, the ADP/O coefficients also boosted under the influence of the isoquinoline alkaloid F-18. It was observed that ADP/O ratio coefficients was expanded within the bonding by the effect of the DHQ-11 conjugate concentration. The concentration of 100 μM dihydroquercetin for FAD-dependent succinate oxidation in rat liver mitochondria surged Chance's respiratory rate by 15% compared to the control. Concentrations of isoquinoline alkaloid F-18 100 μM enlarged the respiratory rate by 13% compared to the control. It was defined that concentrations of 100 μM DHQ-11 conjugate rose the respiratory rate by 20%. The oxidation of FAD-dependent substrates in mitochondria was more actively effected by the DHQ-11 conjugate than dihydroquercetin and F-18 isoquinoline alkaloids.

Published in American Journal of Biomedical and Life Sciences (Volume 10, Issue 3)
DOI 10.11648/j.ajbls.20221003.12
Page(s) 65-69
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Liver, Mitochondria, FAD, ROS, Succinate, Dihydroquercetin, F-18 Isoquinoline Alkaloid, DHQ-11 Conjugate

References
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[3] Biasutto L, Sassi N, Mattarei A, Marotta E, Cattelan P, Toninello A, et al. Impact of mitochondriotropic quercetin derivatives on mitochondria. Biochim. Biophys. Acta 2010; 1797: 189–196. pmid: 19835835.
[4] Bridges H. R., Jones A. Y., Pollak M. N., Hirst J. Effects of metformin and other biguanides on oxidative phosphorylation in mitochondria // Biochem. J. - 2014. - V. 462. - P. 475-487.
[5] Cao, G.; Sophich, E.; Prior, R. L. (1997) Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free Radik Biol Med. 749–760.
[6] Drahota Z., Palenickova E., Endlicher R. et al. Biguanides inhibit complex I, II and IV of rat liver mitochondria and modify their functional properties // Physiol. Res. – 2014. - V. 63 (1). - P. 1-11.
[7] Dzhumaev I. Z., Usmanov P. B., Zhurakulov Sh. N., Vinogradova V. I. (2019) Description of the mechanism of action of alkaloids F-24 and H-14 on the Ca2+ transport systems of cardiomyocytes // Infection, Immunity and Pharmacology No. 3 P. 21-27Dzhumaev I., Usmanov P., Rustamov S., Zhurakulov S.(2020) Comparative inotropic effects of some isoquinoline alkaloids. Biomed Pharmacol J. 13 (1).
[8] Hernandez E.-Aquino and P. Muriel, (2018) Benefits of Naringenin in Liver Disease: Molecular Mechanisms, World Journal of Gastroenterology, vol. 24, no. 16, p. 1679–1707.
[9] Kichinska A., Yarmushkevich V. (2020) Flavonoids and mitochondria: activation of cytoprotective pathways? Molecules 3060 8 out of 22.
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[11] Usmanov P., Dzhumaev I., Rustamov S., Zaripov A., Esimbetov A., Zhurakulov S., Vinogradova V. (2021) Combined inotropic and vasodilating effect of DHA-11, dihydroquercetin flavonoid conjugate with isoquinoline alkaloid 1-aryl-6, 7-dimethoxy-1,2,3,4-tetrahydroisoquinoline. Biomed Pharmacol J; Volume. 14 (2), p. 651-661.
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[13] Weidmann A. E. (2012) Dihydroquercetin: more than just an impurity? European Journal of Pharmacology. Vol. 684, no. 1-3, p. 19-26.
[14] Zhurakulov Sh. N., Babkin V. A., Chernyak E. I., Morozov S. V., Grigorev I. A., Levkovich M. G. and Vinogradova V. I. (2015) Aminomethylation of 1-aryl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinolines by dihydroquercetin. Chemistry of natural compounds. T, 51, №1, 57-61.
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    Ernazarov Zafarjon Mamurovich, Pozilov Mamurjon Komiljonovich, Toshmatova Shoiraxon Ruziyevna, Zhurakulov Sherzod Niyatkobulovich. (2022). Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation. American Journal of Biomedical and Life Sciences, 10(3), 65-69. https://doi.org/10.11648/j.ajbls.20221003.12

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    Ernazarov Zafarjon Mamurovich; Pozilov Mamurjon Komiljonovich; Toshmatova Shoiraxon Ruziyevna; Zhurakulov Sherzod Niyatkobulovich. Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation. Am. J. Biomed. Life Sci. 2022, 10(3), 65-69. doi: 10.11648/j.ajbls.20221003.12

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    AMA Style

    Ernazarov Zafarjon Mamurovich, Pozilov Mamurjon Komiljonovich, Toshmatova Shoiraxon Ruziyevna, Zhurakulov Sherzod Niyatkobulovich. Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation. Am J Biomed Life Sci. 2022;10(3):65-69. doi: 10.11648/j.ajbls.20221003.12

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  • @article{10.11648/j.ajbls.20221003.12,
      author = {Ernazarov Zafarjon Mamurovich and Pozilov Mamurjon Komiljonovich and Toshmatova Shoiraxon Ruziyevna and Zhurakulov Sherzod Niyatkobulovich},
      title = {Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {10},
      number = {3},
      pages = {65-69},
      doi = {10.11648/j.ajbls.20221003.12},
      url = {https://doi.org/10.11648/j.ajbls.20221003.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20221003.12},
      abstract = {In current scientific article, rat liver mitochondria respond to the flavonoids dihydroquercetin (20–100 µM), 1-(2´-bromo-4´,5´-dimethoxyphenyl)-6,7-dimethoxy-1,2,3,4 for respiratory and processes of oxidative phosphorylation - tetrahydroisoquinolines F-18 (20-100 μM) and synthesized on the basis of followings 2-(3,4-dihydroxyphenyl)-6-[1-(2'-bromo-4',5'-dimethoxyphenyl)-6,7- The effect of dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl]methyl-3,5,7-trihydroxychroman-4-on the concentrations of the DHQ-11 conjugate (20-100 μM) was investigated in in vitro experiments. Experiments were conducted within 180-200 gram infertile white male rats. Mitochondrias from rat liver were isolated by the way of differential centrifugation. Concentrations of dihydroquercetin flavonoid 20, 60, 100 μM in FAD-dependent succinate oxidation in rat liver mitochondria were identified to be reliable respiratory control parameters and partially increased ADF/O value, the respiratory control coefficient as Chans demonstrated an increase in respiratory rate under the influence of the alkaloid F-18 isoquinoline 20, 60, 100 μM. Moreover, the ADP/O coefficients also boosted under the influence of the isoquinoline alkaloid F-18. It was observed that ADP/O ratio coefficients was expanded within the bonding by the effect of the DHQ-11 conjugate concentration. The concentration of 100 μM dihydroquercetin for FAD-dependent succinate oxidation in rat liver mitochondria surged Chance's respiratory rate by 15% compared to the control. Concentrations of isoquinoline alkaloid F-18 100 μM enlarged the respiratory rate by 13% compared to the control. It was defined that concentrations of 100 μM DHQ-11 conjugate rose the respiratory rate by 20%. The oxidation of FAD-dependent substrates in mitochondria was more actively effected by the DHQ-11 conjugate than dihydroquercetin and F-18 isoquinoline alkaloids.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation
    AU  - Ernazarov Zafarjon Mamurovich
    AU  - Pozilov Mamurjon Komiljonovich
    AU  - Toshmatova Shoiraxon Ruziyevna
    AU  - Zhurakulov Sherzod Niyatkobulovich
    Y1  - 2022/05/24
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ajbls.20221003.12
    DO  - 10.11648/j.ajbls.20221003.12
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 65
    EP  - 69
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20221003.12
    AB  - In current scientific article, rat liver mitochondria respond to the flavonoids dihydroquercetin (20–100 µM), 1-(2´-bromo-4´,5´-dimethoxyphenyl)-6,7-dimethoxy-1,2,3,4 for respiratory and processes of oxidative phosphorylation - tetrahydroisoquinolines F-18 (20-100 μM) and synthesized on the basis of followings 2-(3,4-dihydroxyphenyl)-6-[1-(2'-bromo-4',5'-dimethoxyphenyl)-6,7- The effect of dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl]methyl-3,5,7-trihydroxychroman-4-on the concentrations of the DHQ-11 conjugate (20-100 μM) was investigated in in vitro experiments. Experiments were conducted within 180-200 gram infertile white male rats. Mitochondrias from rat liver were isolated by the way of differential centrifugation. Concentrations of dihydroquercetin flavonoid 20, 60, 100 μM in FAD-dependent succinate oxidation in rat liver mitochondria were identified to be reliable respiratory control parameters and partially increased ADF/O value, the respiratory control coefficient as Chans demonstrated an increase in respiratory rate under the influence of the alkaloid F-18 isoquinoline 20, 60, 100 μM. Moreover, the ADP/O coefficients also boosted under the influence of the isoquinoline alkaloid F-18. It was observed that ADP/O ratio coefficients was expanded within the bonding by the effect of the DHQ-11 conjugate concentration. The concentration of 100 μM dihydroquercetin for FAD-dependent succinate oxidation in rat liver mitochondria surged Chance's respiratory rate by 15% compared to the control. Concentrations of isoquinoline alkaloid F-18 100 μM enlarged the respiratory rate by 13% compared to the control. It was defined that concentrations of 100 μM DHQ-11 conjugate rose the respiratory rate by 20%. The oxidation of FAD-dependent substrates in mitochondria was more actively effected by the DHQ-11 conjugate than dihydroquercetin and F-18 isoquinoline alkaloids.
    VL  - 10
    IS  - 3
    ER  - 

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Author Information
  • Department of Biophysics, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan

  • Department of Biophysics, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan

  • Department of Biology, Faculty of Natural Sciences, Kokand State Pedagogical Institute Named After Muqimi, Kokand, Uzbekistan

  • Department of Biophysics, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan

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