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Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats

Received: 11 November 2020     Accepted: 24 November 2020     Published: 4 December 2020
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Abstract

The state of the mitochondrial megapore (mitochondrial permeability transition pore-mPTP), respiration and oxidative phosphorylation of rat liver and pancreas mitochondria in streptozotocin (STZ) - induced diabetes were studied, considered the ways of correction of the detected membrane damage with the flavone luteolin isolated from the plant Inula caspica. It was shown that, under conditions of experimental diabetes mellitus, the rate of swelling of rat liver and pancreas mitochondria is higher than of the healthy ones; this means that mPTP of rat liver and pancreas mitochondria is in the open state in pathology. Luteolin recovers mPTP to the normal condition, thus removing the effect of STZ on mitochondria. It was also shown that, the respiration rate of liver and pancreatic mitochondria in the state 3 and state 4 states increases in STZ - induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The data obtained indicate the disconnection of respiration and oxidative phosphorylation in STZ - induced diabetes. Luteolin (oral dose is 50 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver and pancreas mitochondria, probably due to its antioxidant properties.

Published in American Journal of Biomedical and Life Sciences (Volume 8, Issue 6)
DOI 10.11648/j.ajbls.20200806.15
Page(s) 220-224
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Liver, Pancreas, Mitochondria, mPTP, Lipid Peroxidation, Streptozotocin-induced Diabetes, Luteolin, Oxidative Phosphorylation

References
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[3] Asrarov M. I., Pozilov M. K., Rahmatullaeva M. M. and Ergaschev N. A. (2014). The influence of the hypoglycemic agent glycorazmulin on the functional state of mitochondria in the rats with streptozotocin-induced diabetes, Problems of Endocrinology 60 (3): 38–42.
[4] Newsholme P., Cruzat V F., Keane K N., Carlessi R. and Homem de Bittencourt P. I. (2016). Molecular mechanisms of ROS production and oxidative stress in diabetes, Biochem J, 473 (24): 4527-4550.
[5] Roy J., Galano J. M., Durand T., Le Guennec J. Y. and Chung‐Yung Lee J. (2017). Physiological role of reactive oxygen species as promoters of natural defenses, The Faseb Journal, 31 (9): 3729-3745.
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[8] Newsholme P., Keane K. N., Carlessi R. and Cruzat V. (2019). Oxidative stress pathways in pancreatic β-cells and insulin-sensitive cells and tissues: importance to cell metabolism, function, and dysfunction, Am J Physiol Cell Physiol, 317. (3): 420-433.
[9] Schneider W. C., Hageboom G. H. and Pallade G. E. (1948). Cytochemical studies of mammalian tissues; isolation of intact mitochondria from rat liver; some biochemical properties of mitochondria and submicroscopic particulate material, J. Biol. Chem, 172 (2): 619–635.
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[14] Pozilov M. K., Asrarov M. I., Urmanova G. U. and Eshbakova K. A. (2015) Protective effect of salvifolin on liver mitochondrial function in rats with experimental diabetes, European science review, 7-8: 3–7.
[15] Asrarov M. I., Pozilov M. K. and Ergashev N. A. (2015) Effect of a hypoglycemic agent glycorazmulin on the mitochondrial permeability transition pore at the alloxan-induced diabetes, The congress Euromedica-Hanover. Modern Aspects of diagnostics, treatment and rehabilitation, 12–13.
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    Pozilov Mamurjon Komiljonovich, Ernazarov Zafar Mamurovich, Afzalova Sayyora Abdulahadovna, Asrarov Muzaffar Islamovich, Ergashev Nurali Azamovich, et al. (2020). Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats. American Journal of Biomedical and Life Sciences, 8(6), 220-224. https://doi.org/10.11648/j.ajbls.20200806.15

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    ACS Style

    Pozilov Mamurjon Komiljonovich; Ernazarov Zafar Mamurovich; Afzalova Sayyora Abdulahadovna; Asrarov Muzaffar Islamovich; Ergashev Nurali Azamovich, et al. Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats. Am. J. Biomed. Life Sci. 2020, 8(6), 220-224. doi: 10.11648/j.ajbls.20200806.15

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    AMA Style

    Pozilov Mamurjon Komiljonovich, Ernazarov Zafar Mamurovich, Afzalova Sayyora Abdulahadovna, Asrarov Muzaffar Islamovich, Ergashev Nurali Azamovich, et al. Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats. Am J Biomed Life Sci. 2020;8(6):220-224. doi: 10.11648/j.ajbls.20200806.15

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  • @article{10.11648/j.ajbls.20200806.15,
      author = {Pozilov Mamurjon Komiljonovich and Ernazarov Zafar Mamurovich and Afzalova Sayyora Abdulahadovna and Asrarov Muzaffar Islamovich and Ergashev Nurali Azamovich and Komilov Baxrom Jamoldinovich},
      title = {Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {8},
      number = {6},
      pages = {220-224},
      doi = {10.11648/j.ajbls.20200806.15},
      url = {https://doi.org/10.11648/j.ajbls.20200806.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20200806.15},
      abstract = {The state of the mitochondrial megapore (mitochondrial permeability transition pore-mPTP), respiration and oxidative phosphorylation of rat liver and pancreas mitochondria in streptozotocin (STZ) - induced diabetes were studied, considered the ways of correction of the detected membrane damage with the flavone luteolin isolated from the plant Inula caspica. It was shown that, under conditions of experimental diabetes mellitus, the rate of swelling of rat liver and pancreas mitochondria is higher than of the healthy ones; this means that mPTP of rat liver and pancreas mitochondria is in the open state in pathology. Luteolin recovers mPTP to the normal condition, thus removing the effect of STZ on mitochondria. It was also shown that, the respiration rate of liver and pancreatic mitochondria in the state 3 and state 4 states increases in STZ - induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The data obtained indicate the disconnection of respiration and oxidative phosphorylation in STZ - induced diabetes. Luteolin (oral dose is 50 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver and pancreas mitochondria, probably due to its antioxidant properties.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats
    AU  - Pozilov Mamurjon Komiljonovich
    AU  - Ernazarov Zafar Mamurovich
    AU  - Afzalova Sayyora Abdulahadovna
    AU  - Asrarov Muzaffar Islamovich
    AU  - Ergashev Nurali Azamovich
    AU  - Komilov Baxrom Jamoldinovich
    Y1  - 2020/12/04
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ajbls.20200806.15
    DO  - 10.11648/j.ajbls.20200806.15
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 220
    EP  - 224
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20200806.15
    AB  - The state of the mitochondrial megapore (mitochondrial permeability transition pore-mPTP), respiration and oxidative phosphorylation of rat liver and pancreas mitochondria in streptozotocin (STZ) - induced diabetes were studied, considered the ways of correction of the detected membrane damage with the flavone luteolin isolated from the plant Inula caspica. It was shown that, under conditions of experimental diabetes mellitus, the rate of swelling of rat liver and pancreas mitochondria is higher than of the healthy ones; this means that mPTP of rat liver and pancreas mitochondria is in the open state in pathology. Luteolin recovers mPTP to the normal condition, thus removing the effect of STZ on mitochondria. It was also shown that, the respiration rate of liver and pancreatic mitochondria in the state 3 and state 4 states increases in STZ - induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The data obtained indicate the disconnection of respiration and oxidative phosphorylation in STZ - induced diabetes. Luteolin (oral dose is 50 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver and pancreas mitochondria, probably due to its antioxidant properties.
    VL  - 8
    IS  - 6
    ER  - 

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Author Information
  • Institute of Biophysics and Biochemistry at the National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan

  • Institute of Biophysics and Biochemistry at the National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan

  • Department of Human and Animal Physiology, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan

  • Institute of Biophysics and Biochemistry at the National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan

  • Institute of Biophysics and Biochemistry at the National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan

  • Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan

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