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Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria

Received: 18 March 2020     Accepted: 14 April 2020     Published: 9 June 2020
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Abstract

Malaria is a serious public health problem, this study was aimed at comparing Microscopy and Rapid Diagnostics test in malaria parasite detection in patients in some hospitals in Sokoto, Nigeria. Blood Samples were collected by venipuncture and dispensed into EDTA bottle. Thick blood films were made by adding few drops of anticoagulated blood on a clean glass slides and emulsifying to coin size using a spreader and allowed to air-dry and stained with 10% Geimsa stain for 10 minutes, allowed to air-dry and examined using ×100 objective to confirm the presence of malaria parasites. Of the 100 patients screened, 37% and 24% were positive for malaria using Carestart and SD-Bioline, while 53% were malaria positive by microscopy. Prevalence of malaria parasite by sex was 53.6% and 52.3% in females and males. Malaria prevalence by age was highest among patients aged 11-20, while the lowest prevalence was observed in patients aged 51-60 years. Based on occupation, the prevalence of malaria was highest among unemployed patients 58.5%, followed by 22.6% prevalence of malaria among business men and women. No significant difference was observed in malaria infection by sex, age and occupations P<0.05. The sensitivity and specificity of Carestart and SD-Bioline screening kits were 54.7% and 82.6%, 34.6 and 87.2%. The predictive positive value of Carestart was 78.4% while its negative predictive value was 61.3% in contrast to SD-Bioline with 75% and 54.7% as the positive predictive value and negative predictive value. Rapid diagnostic test are suitable alternatives to microscopy particularly in resources limited rural areas.

Published in American Journal of Biomedical and Life Sciences (Volume 8, Issue 4)
DOI 10.11648/j.ajbls.20200804.11
Page(s) 64-68
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Comparison, Microscopic, Rapid Test, Malaria, Sokoto

References
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  • APA Style

    Garba Ibrahim, Umar Asiya Imam, Ganau Ahmed Mohammed, Raji Mudasiru Iyanda Omowale, Fana Sani Abdullahi, et al. (2020). Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria. American Journal of Biomedical and Life Sciences, 8(4), 64-68. https://doi.org/10.11648/j.ajbls.20200804.11

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    ACS Style

    Garba Ibrahim; Umar Asiya Imam; Ganau Ahmed Mohammed; Raji Mudasiru Iyanda Omowale; Fana Sani Abdullahi, et al. Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria. Am. J. Biomed. Life Sci. 2020, 8(4), 64-68. doi: 10.11648/j.ajbls.20200804.11

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    AMA Style

    Garba Ibrahim, Umar Asiya Imam, Ganau Ahmed Mohammed, Raji Mudasiru Iyanda Omowale, Fana Sani Abdullahi, et al. Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria. Am J Biomed Life Sci. 2020;8(4):64-68. doi: 10.11648/j.ajbls.20200804.11

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  • @article{10.11648/j.ajbls.20200804.11,
      author = {Garba Ibrahim and Umar Asiya Imam and Ganau Ahmed Mohammed and Raji Mudasiru Iyanda Omowale and Fana Sani Abdullahi and Shinkafi Sa’adatu Aliyu and Kazeem Ademola},
      title = {Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {8},
      number = {4},
      pages = {64-68},
      doi = {10.11648/j.ajbls.20200804.11},
      url = {https://doi.org/10.11648/j.ajbls.20200804.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20200804.11},
      abstract = {Malaria is a serious public health problem, this study was aimed at comparing Microscopy and Rapid Diagnostics test in malaria parasite detection in patients in some hospitals in Sokoto, Nigeria. Blood Samples were collected by venipuncture and dispensed into EDTA bottle. Thick blood films were made by adding few drops of anticoagulated blood on a clean glass slides and emulsifying to coin size using a spreader and allowed to air-dry and stained with 10% Geimsa stain for 10 minutes, allowed to air-dry and examined using ×100 objective to confirm the presence of malaria parasites. Of the 100 patients screened, 37% and 24% were positive for malaria using Carestart and SD-Bioline, while 53% were malaria positive by microscopy. Prevalence of malaria parasite by sex was 53.6% and 52.3% in females and males. Malaria prevalence by age was highest among patients aged 11-20, while the lowest prevalence was observed in patients aged 51-60 years. Based on occupation, the prevalence of malaria was highest among unemployed patients 58.5%, followed by 22.6% prevalence of malaria among business men and women. No significant difference was observed in malaria infection by sex, age and occupations P<0.05. The sensitivity and specificity of Carestart and SD-Bioline screening kits were 54.7% and 82.6%, 34.6 and 87.2%. The predictive positive value of Carestart was 78.4% while its negative predictive value was 61.3% in contrast to SD-Bioline with 75% and 54.7% as the positive predictive value and negative predictive value. Rapid diagnostic test are suitable alternatives to microscopy particularly in resources limited rural areas.},
     year = {2020}
    }
    

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    T1  - Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria
    AU  - Garba Ibrahim
    AU  - Umar Asiya Imam
    AU  - Ganau Ahmed Mohammed
    AU  - Raji Mudasiru Iyanda Omowale
    AU  - Fana Sani Abdullahi
    AU  - Shinkafi Sa’adatu Aliyu
    AU  - Kazeem Ademola
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    DO  - 10.11648/j.ajbls.20200804.11
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
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    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20200804.11
    AB  - Malaria is a serious public health problem, this study was aimed at comparing Microscopy and Rapid Diagnostics test in malaria parasite detection in patients in some hospitals in Sokoto, Nigeria. Blood Samples were collected by venipuncture and dispensed into EDTA bottle. Thick blood films were made by adding few drops of anticoagulated blood on a clean glass slides and emulsifying to coin size using a spreader and allowed to air-dry and stained with 10% Geimsa stain for 10 minutes, allowed to air-dry and examined using ×100 objective to confirm the presence of malaria parasites. Of the 100 patients screened, 37% and 24% were positive for malaria using Carestart and SD-Bioline, while 53% were malaria positive by microscopy. Prevalence of malaria parasite by sex was 53.6% and 52.3% in females and males. Malaria prevalence by age was highest among patients aged 11-20, while the lowest prevalence was observed in patients aged 51-60 years. Based on occupation, the prevalence of malaria was highest among unemployed patients 58.5%, followed by 22.6% prevalence of malaria among business men and women. No significant difference was observed in malaria infection by sex, age and occupations P<0.05. The sensitivity and specificity of Carestart and SD-Bioline screening kits were 54.7% and 82.6%, 34.6 and 87.2%. The predictive positive value of Carestart was 78.4% while its negative predictive value was 61.3% in contrast to SD-Bioline with 75% and 54.7% as the positive predictive value and negative predictive value. Rapid diagnostic test are suitable alternatives to microscopy particularly in resources limited rural areas.
    VL  - 8
    IS  - 4
    ER  - 

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Author Information
  • Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria

  • Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria

  • Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria

  • Department of Pharmaceutics and Pharm, Microbiology, Usmanu Danfodiyo University, Sokoto, Nigeria

  • Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria

  • Department of Biological Sciences, Federal University, Gusau, Nigeria

  • Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria

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