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Correlation Between Serum PON1 Arylesterase Activity and Rs 854573 PON1 A < G Polymorphism with Type 2 Diabetes in an Eastern Indian Cohort

Received: 28 September 2019     Accepted: 25 October 2019     Published: 31 October 2019
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Abstract

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder of glucose and lipids and characterized by defect in insulin secretion or action. Oxidative imbalance has also been implicated in the etiology of diabetes. Paraoxonase-1 (PON1) is an esterase and lactonase which is found in the circulation bound to high-density lipoproteins (HDL). Alterations and associations of circulating PON1 levels with a variety of diseases including diabetes encourages us to investigate the possible association between PON1 A/G rs854573 polymorphism and serum PON1 activity with T2DM. The study essentially follows a population based case-control format with 101 diabetic and 102 healthy controls. The findings revealed association of polymorphism with the diseased status (p value 0.0002, OR 3.49, 95% CI 1.77 to 6.9). With significantly higher range of mean serum PON1 Arylesterase (AREase) activity in control (9.99 – 0.96 kU/L) than in diabetic patients (5.25 - 0.508 kU/L) (p value,<0.001), a large difference between common diabetic AA genotype and combined diabetic heterozygous and homozygous genotypes (AG+GG) for risk allele G (assymptometic p value,<0.001), or in between two AA genotypes (Diabetic/Non diabetic, p<0.001), was explored by parametric and non parametric statistical pairwise comparison. Serum PON1 activity was found to be independent of other clinical factors such as plasma glucose levels. Western blot analysis of serum samples detected a significant difference of PON1 proteins in diabetic patients and control subjects (p value 0.008). In conclusion serum PON1 AREase activity which to an extent correlated with PON1 promoter polymorphism might be a good predictor of the disease risk.

Published in American Journal of Biomedical and Life Sciences (Volume 7, Issue 6)
DOI 10.11648/j.ajbls.20190706.11
Page(s) 121-132
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

AREase Activity, Oxidative Stress, Polymorphism, PON1, T2DM, Western Blot

References
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    Suranjana Ray Haldar, Atish Haldar, Ipshit Mishra, Arpita Chakarabarty, Sanghamitra Sengupta, et al. (2019). Correlation Between Serum PON1 Arylesterase Activity and Rs 854573 PON1 A Polymorphism with Type 2 Diabetes in an Eastern Indian Cohort. American Journal of Biomedical and Life Sciences, 7(6), 121-132. https://doi.org/10.11648/j.ajbls.20190706.11

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    Suranjana Ray Haldar; Atish Haldar; Ipshit Mishra; Arpita Chakarabarty; Sanghamitra Sengupta, et al. Correlation Between Serum PON1 Arylesterase Activity and Rs 854573 PON1 A Polymorphism with Type 2 Diabetes in an Eastern Indian Cohort. Am. J. Biomed. Life Sci. 2019, 7(6), 121-132. doi: 10.11648/j.ajbls.20190706.11

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    AMA Style

    Suranjana Ray Haldar, Atish Haldar, Ipshit Mishra, Arpita Chakarabarty, Sanghamitra Sengupta, et al. Correlation Between Serum PON1 Arylesterase Activity and Rs 854573 PON1 A Polymorphism with Type 2 Diabetes in an Eastern Indian Cohort. Am J Biomed Life Sci. 2019;7(6):121-132. doi: 10.11648/j.ajbls.20190706.11

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  • @article{10.11648/j.ajbls.20190706.11,
      author = {Suranjana Ray Haldar and Atish Haldar and Ipshit Mishra and Arpita Chakarabarty and Sanghamitra Sengupta and Maitree Bhattacharyya},
      title = {Correlation Between Serum PON1 Arylesterase Activity and Rs 854573 PON1 A  Polymorphism with Type 2 Diabetes in an Eastern Indian Cohort},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {7},
      number = {6},
      pages = {121-132},
      doi = {10.11648/j.ajbls.20190706.11},
      url = {https://doi.org/10.11648/j.ajbls.20190706.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20190706.11},
      abstract = {Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder of glucose and lipids and characterized by defect in insulin secretion or action. Oxidative imbalance has also been implicated in the etiology of diabetes. Paraoxonase-1 (PON1) is an esterase and lactonase which is found in the circulation bound to high-density lipoproteins (HDL). Alterations and associations of circulating PON1 levels with a variety of diseases including diabetes encourages us to investigate the possible association between PON1 A/G rs854573 polymorphism and serum PON1 activity with T2DM. The study essentially follows a population based case-control format with 101 diabetic and 102 healthy controls. The findings revealed association of polymorphism with the diseased status (p value 0.0002, OR 3.49, 95% CI 1.77 to 6.9). With significantly higher range of mean serum PON1 Arylesterase (AREase) activity in control (9.99 – 0.96 kU/L) than in diabetic patients (5.25 - 0.508 kU/L) (p value,PON1 promoter polymorphism might be a good predictor of the disease risk.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Correlation Between Serum PON1 Arylesterase Activity and Rs 854573 PON1 A  Polymorphism with Type 2 Diabetes in an Eastern Indian Cohort
    AU  - Suranjana Ray Haldar
    AU  - Atish Haldar
    AU  - Ipshit Mishra
    AU  - Arpita Chakarabarty
    AU  - Sanghamitra Sengupta
    AU  - Maitree Bhattacharyya
    Y1  - 2019/10/31
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ajbls.20190706.11
    DO  - 10.11648/j.ajbls.20190706.11
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 121
    EP  - 132
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20190706.11
    AB  - Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder of glucose and lipids and characterized by defect in insulin secretion or action. Oxidative imbalance has also been implicated in the etiology of diabetes. Paraoxonase-1 (PON1) is an esterase and lactonase which is found in the circulation bound to high-density lipoproteins (HDL). Alterations and associations of circulating PON1 levels with a variety of diseases including diabetes encourages us to investigate the possible association between PON1 A/G rs854573 polymorphism and serum PON1 activity with T2DM. The study essentially follows a population based case-control format with 101 diabetic and 102 healthy controls. The findings revealed association of polymorphism with the diseased status (p value 0.0002, OR 3.49, 95% CI 1.77 to 6.9). With significantly higher range of mean serum PON1 Arylesterase (AREase) activity in control (9.99 – 0.96 kU/L) than in diabetic patients (5.25 - 0.508 kU/L) (p value,PON1 promoter polymorphism might be a good predictor of the disease risk.
    VL  - 7
    IS  - 6
    ER  - 

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Author Information
  • Department of Biochemistry, University of Calcutta, Kolkata, India

  • Department of ENT, Calcutta National Medical College, Kolkata, India

  • Department of Surgery, Calcutta National Medical College, Kolkata, India

  • Institute of Postgraduate Medical Education and Research, Government of West Bengal, Kolkata, India

  • Department of Biochemistry, University of Calcutta, Kolkata, India

  • Department of Biochemistry, University of Calcutta, Kolkata, India

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