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Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat

Received: 19 February 2016     Accepted: 6 March 2016     Published: 28 March 2016
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Abstract

Cellulase are the enzymes hydrolyzing cellulosic biomass and are produced by the microorganism that grown over cellulosic matter. Bacterial Cellulases poses more advantage when compared to the cellulases from other sources. This study aims to determine the optimum conditions of cellulase production from the bacteria Bacillus Circulans CC2 and CC4 in comparison with isolates hydrolize rice straw. CC2 and CC4 isolated from larvae Cossus cossus. The testing to produce cellulase was done with various pH and temperature. The enzyme activity was tested using DNS Method. The results showed that the isolated CC2 and CC4 have the same optimum temperature of 70C on condition CMC 1%, medium pH 7,0. Crude extract cellulase isolates CC2 work optimally at pH 4 while the isolates CC4 work at pH 7. highest activity in the cellulase enzymes hydrolyze the substrate on cellulose powder with optimum activity at pH 4 CC4 isolates of 2.775 x 10-2 U/ mL, while the enzyme activity CC2 isolates 4.359 x 10-2 U/mL, p this occurs due to the enzymatic hydrolysis of cellulosic substrates with cellulase enzymes will outline the cellulose into glucose which is a simple form.

Published in American Journal of Biomedical and Life Sciences (Volume 4, Issue 2)
DOI 10.11648/j.ajbls.20160402.13
Page(s) 21-25
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Cellulace, Cossus Cossus, Rice Straw

References
[1] Delalibera, I., Jo H., and Kenneth F. R. 2005. Contrast In Cellulolytic of Gut Mikcroorganisms Between The Wood Borer, Saperda vestita (Coleoptera: Cerambiydae), and The Bark Beetles, Ips pini and Dendroctonus frontalis (Coleoptera: Curculionidae). Physiological Ecologhy Entomol 34(3): 541-547.
[2] Anindyawati, Trisanti. “Prospek Enzim Dan Limbah Lignoselulosa Untuk Produksi Bioetanol”. Pusat Penelitian Bioteknologi-LIPI, 2009.
[3] Baharuddin, M., Patong, R., Ahmad, A., La Nafie, N., 2014. Pengaruh Suhu dan pH terhadap hidrolisis CMC oleh enzim selulase dari isolat bakteri larva Cossus cossus. J Teknosasins dan Teknologi 8(3): 343-356.
[4] Baharuddin, M., Patong, R., Ahmad, A., La Nafie, N., 2014. Isolasi dan Karakterisasi Bakteri Simbion Larva Kupu-kupu Cossus cossus Penghasil Selulase. Al-Kimia Jurnal Penelitian sains Kimia 2(2).
[5] Bai, S., M. Ravi K., D. J. Mukesh K.,. Balashanmugan., M. D. Balakumaran., P. T. Kalaicchevlan. 2012. Cellulase Production by Bacillus subtilis Isolatd from Cow Dung. Archives of Applied Science Research, 4(1): 269-279.
[6] Chandel, A., and Sigh O. V. 2011. Weedy Lignocellulosic feedstock and Microbial metabolic engineering: advancing the generation of Biofuel. Appl Microbiol Biotechnol 89: 1289-1303.
[7] Abdelnasser, S. S., Ahmed I., 2007. Isolation and Identificatio of New Cellulases Producing Thermophilic Bacteria From an Eghyptian Hot Spring and some Properties of Crude enzyme. Australian J. of Basic and Applied Science, 1(4): 473-478.
[8] Lynd, L. R., Willem P. J., Willem H. V. Z., and Isak S. P. 2002. Microbial Cellulose Utilization: Fundamental and Biotechnology. Microbiology and Molecular Biology Reviews, 66(3) 506-577.
[9] Willis, J. D., Cris O., and Juan L. J. 2010. Methods for Discovery and Characterization of Cellulolytic Enzymes from Insects, Journal compilation, Insect Science 17: 184-198.
[10] Lawrence, J. F., and Britton E. B. 1991. The Insects of Australian. A text Book for Student and Research Worker. Vol. 2. Cornell Universty Press Ithaca: New York.
[11] Kalluri, U. C., and Keller M. 2012. Bioenergy Research: A New Paradigm in Multidiciplinary research. Review J. The Royal Society Interface 7. 1391-1401.
[12] Meryandini, Anja., 2009. Isolasi Bakteri Selulolitik dan Karakterisasi Enzimnya, Makara sains 13(1) 2009 h 36.
[13] Larasati, Raissa. “Sintesis Fase Diam Selulosa-g-AAM Dengan Berbagai Kadar Penaut-Silang Sebagai Media Separator Xantorizol Dari Ekstrak Temu Lawak”. Skripsi. Bogor: Departeman Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam IPB, 2012.
[14] Vilanova, C., Marco G., Laura D., Salvador G., Vicente S., Esther B., Daniel R., Manuel P. 2012. Bacteria from acidic to strongly alkaline insect midguts: Potensial sources of extreme cellulolytic enzymes. Biomass & Bioenergy 45: 288-294.
[15] Geib, S. M., Filley, T. R., Hatcher, P. G., Hoover, K., Carlson, J. E., Jimenez-Gasco Mdel, M., Nakagawa-Izumi, A., Sleighter, R. L. and Tien, M. 2008. Lignin degradation in wood-feeding insects. Proceedings of the National Academy of Sciences 105: 12932–12937.
[16] Purwadaria, T., Marbun, P. A., Arnold P. S., Pius P. K. 2003. Perbandingan Aktivitas Enzim Selulase dari Bakteri dan Kapang Hasil Isolasi dari Rayap. 8(4): 213-219.
[17] Shakar, T., Isairastu L., Cellulase production by Bacillus pumilus EWBCMI under Varying Cultural Conditions. Middle-East J. Sci. Res., 8(1): 40-45, 2011.
[18] Standar Nasional Indonesia. “Cara Uji Kadar Lignin 0492-2008”. Badan Standar Nasional, 2008.
[19] Standar Nasional Indonesia. “Cara Uji Kadar Selulosa, α-selulosa, β-selulosa dan γ-selulosa 0444-2009”. Badan Standar Nasional, 2009.
[20] Irfan, M., Safdar A., Quratuain S., Muhammad N. 2012. Isolation and Screening of Cellulolytic Bacteria from Soil and Optimization of Cellulase Production and Activity. Journal of Biochemistry 37(3): 287-293.
Cite This Article
  • APA Style

    Maswati Baharuddin, Ahyar Ahmad, Nursiah La Nafie, Firdaus Zenta. (2016). Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat. American Journal of Biomedical and Life Sciences, 4(2), 21-25. https://doi.org/10.11648/j.ajbls.20160402.13

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    ACS Style

    Maswati Baharuddin; Ahyar Ahmad; Nursiah La Nafie; Firdaus Zenta. Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat. Am. J. Biomed. Life Sci. 2016, 4(2), 21-25. doi: 10.11648/j.ajbls.20160402.13

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    AMA Style

    Maswati Baharuddin, Ahyar Ahmad, Nursiah La Nafie, Firdaus Zenta. Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat. Am J Biomed Life Sci. 2016;4(2):21-25. doi: 10.11648/j.ajbls.20160402.13

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  • @article{10.11648/j.ajbls.20160402.13,
      author = {Maswati Baharuddin and Ahyar Ahmad and Nursiah La Nafie and Firdaus Zenta},
      title = {Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {4},
      number = {2},
      pages = {21-25},
      doi = {10.11648/j.ajbls.20160402.13},
      url = {https://doi.org/10.11648/j.ajbls.20160402.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160402.13},
      abstract = {Cellulase are the enzymes hydrolyzing cellulosic biomass and are produced by the microorganism that grown over cellulosic matter. Bacterial Cellulases poses more advantage when compared to the cellulases from other sources. This study aims to determine the optimum conditions of cellulase production from the bacteria Bacillus Circulans CC2 and CC4 in comparison with isolates hydrolize rice straw. CC2 and CC4 isolated from larvae Cossus cossus. The testing to produce cellulase was done with various pH and temperature. The enzyme activity was tested using DNS Method. The results showed that the isolated CC2 and CC4 have the same optimum temperature of 70C on condition CMC 1%, medium pH 7,0. Crude extract cellulase isolates CC2 work optimally at pH 4 while the isolates CC4 work at pH 7. highest activity in the cellulase enzymes hydrolyze the substrate on cellulose powder with optimum activity at pH 4 CC4 isolates of 2.775 x 10-2 U/ mL, while the enzyme activity CC2 isolates 4.359 x 10-2 U/mL, p this occurs due to the enzymatic hydrolysis of cellulosic substrates with cellulase enzymes will outline the cellulose into glucose which is a simple form.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat
    AU  - Maswati Baharuddin
    AU  - Ahyar Ahmad
    AU  - Nursiah La Nafie
    AU  - Firdaus Zenta
    Y1  - 2016/03/28
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajbls.20160402.13
    DO  - 10.11648/j.ajbls.20160402.13
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 21
    EP  - 25
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20160402.13
    AB  - Cellulase are the enzymes hydrolyzing cellulosic biomass and are produced by the microorganism that grown over cellulosic matter. Bacterial Cellulases poses more advantage when compared to the cellulases from other sources. This study aims to determine the optimum conditions of cellulase production from the bacteria Bacillus Circulans CC2 and CC4 in comparison with isolates hydrolize rice straw. CC2 and CC4 isolated from larvae Cossus cossus. The testing to produce cellulase was done with various pH and temperature. The enzyme activity was tested using DNS Method. The results showed that the isolated CC2 and CC4 have the same optimum temperature of 70C on condition CMC 1%, medium pH 7,0. Crude extract cellulase isolates CC2 work optimally at pH 4 while the isolates CC4 work at pH 7. highest activity in the cellulase enzymes hydrolyze the substrate on cellulose powder with optimum activity at pH 4 CC4 isolates of 2.775 x 10-2 U/ mL, while the enzyme activity CC2 isolates 4.359 x 10-2 U/mL, p this occurs due to the enzymatic hydrolysis of cellulosic substrates with cellulase enzymes will outline the cellulose into glucose which is a simple form.
    VL  - 4
    IS  - 2
    ER  - 

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Author Information
  • Departement of Chemistry, Islamic State of Alauddin Universty, South Sulawesi, Indonesia

  • Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar, South Sulawesi, Indonesia

  • Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar, South Sulawesi, Indonesia

  • Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar, South Sulawesi, Indonesia

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