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Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection

Received: 16 March 2015     Accepted: 31 March 2015     Published: 30 April 2015
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Abstract

Diagnosis of tuberculosis from different body fluids remains challenging due to various limitations of the conventional and molecular methods. We studied the role of adenosine deaminase (ADA) assay to diagnose tubercular infection in cerebrospinal fluid, peritoneal fluid and pleural fluid. Fifty three patients with tubercular meningitis, peritonitis and pleuritis were enrolled in this study on the basis of clinical, radiological, cytological, biochemical and somewhere bacteriological evidences. Cases positive by AFB smear, culture or PCR were considered as confirmed TB and other as probable TB cases. Another 28 non-TB cases were included as control. In 53 suspected TB cases ADA was found positive in highest 42 (79.2%) cases, whereas smear and/ culture in 10 (18.7%) and PCR in 18 (33.9%) cases. ADA assay revealed 100% positivity in confirmed TB cases and 14.3% in non TB cases. The sensitivity and specificity of ADA was found 79% and 86% respectively when the cut off value was used ≥ 10 IU/L for CSF and ≥ 40 IU/L for pleural or peritoneal fluid. Mean ADA values of confirmed and probable TB cases were found significantly higher than that of non TB cases (< 0.05). Significance of difference was determined by ANOVA and Kruskal-Wallis test. Thus, adenosine deaminase assay in body fluids has proved to be efficient, reliable and simple method to diagnose tubercular meningitis, peritonitis and pleuritis.

Published in American Journal of Biomedical and Life Sciences (Volume 3, Issue 3)
DOI 10.11648/j.ajbls.20150303.14
Page(s) 49-54
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Body Fluid, Tuberculosis, Adenosine Deaminase Assay

References
[1] WHO 2012 Report: TB in South East Asia - country profile: Bangladesh. World Health Organization, Geneva 2012.
[2] J.B. Mehta, A. Dutt, L. Harvill and K.M. Mathews, “Epidemiology of extrapulmonary tuberculosis: a comparative analysis with pre-AIDS era,” Chest, vol. 99, pp. 1134-1138, 1991.
[3] A.A. Lazarus, S. McKay, R. Gilbert, “Pleural tuberculosis,” Dis Mon, vol. 53, pp. 16-21, 2007.
[4] P.C. Wong, “Management of tuberculous pleuritis: can we do better?,” Respirology, vol. 10, pp. 144-8, 2005.
[5] F.M. Sanai, K.I.Bzeizi, “Systematic review: tuberculous peritonitis--presenting features, diagnostic strategies and treatment,” Aliment Pharmacol Ther, vol. 22, pp. 685-700, 2005.
[6] S. Ahmed, R. Fatema, A.A. Saleh, H. Sattar and M.R.A. Miah, “Diagnostic significance of pleural fluid adenosine Deaminase activity in tuberculous pleurisy,” Ibrahim Med. Coll J, vol. 5(1), pp. 1-5, 2011.
[7] S.K. Sharma, A. Mohan, “Extrapulmonary tuberculosis – Review article,” Indian J Med Res, vol. 120, pp. 316-53, 2004.
[8] C.E. Bueno, M.G. Clemente, B.C. Castro, L.M. Martín, S.R. Ramos, A.G. Panizo, J.M. Glez-Río, “Cytologic and bacteriologic analyzes of fluid and pleural biopsy with cop’s needle,” Arch Intern Med, vol. 150 (6), pp. 1190-1194, 1990.
[9] P. Mrinal, D. Subinay, “Adenosine deaminase and its isoenzyme as a diagnostic marker in tubercular pleural effusion,” J Drug Del Ther, vol. 4(1), pp. 18-21, 2014.
[10] S.K. Sharma, M. Tahir, A. Mohan, D. Smith-rohrberg, H.K. Mishra, R.M. Pandey, “Diagnostic accuracy of ascitic fluid INF-γ and adenosine deaminase assays in the diagnosis of tuberculous ascites,” J Interferon & Cytokine Res, vol. 26, pp. 484–488, 2006.
[11] P.D. Davies, B. Chakrabarti, P. Albert, “Latest development in the diagnosis of tuberculosis,” European Infec Dis, pp. 75-76, 2008.
[12] M. Caws, S.M. Wilson, C. Clough, F. Drobniewski, “Role of IS6110-Targeted PCR, Culture, Biochemical, Clinical, and Immunological Criteria for Diagnosis of Tuberculous Meningitis,” Clin Microbiol, vol. 38, pp. 3150-3155, 2000.
[13] L. Boonyagars, S. Kiertiburanakul, “Use of Adenosine Deaminase for the Diagnosis of Tuberculosis: A Review,” J Infect Dis Antimicrob Agents, vol. 27(2), pp. 111-118, 2010.
[14] D. Song, A.R. Lun, W. Chiu, “Diazyme adenosine deaminase in the diagnosis of tuberculous pleural effusion: method evaluation and clinical experiences in a New Zealand population,” NZ J Med Lab Science, vol. 64, pp. 11-13, 2010.
[15] M.C. Feres, M.C. Martino, S. Maldijian, F. Batista, J.A. Gabriel, S. Tufik, “Laboratorial validation of an automated assay for the determination of adenosine deaminase activity in pleural fluid and cerebrospinal fluid,” J Bras Pneumol, vol. 34, pp. 1033-9, 2008.
[16] R.S. Kashyap, R.P. Kainthla, A.V. Mudaliar, H.J. Purohit, G.M. Taori and H.F. Daginawala, “Cerebrospinal fluid adenosine deaminase activity: A complimentary tool in the early diagnosis of tuberculous meningitis,” Cereb Fluid Res, vol. 3, pp. 5, 2006.
[17] I. Ocana, J.M. Martinez-Vazquez, R.M. Segura, T. Fernandez-De-Sevilla and J.A. Capdevila, “Adenosine Deaminase in Pleural Fluids,” Chest, vol. 84(1). pp. 51-53, 1983.
[18] J.M. Martinez-Vazquez, I. Ocana, E. Ribera, R.M. Segura and C. Pascual, “Adenosine deaminase activity in the diagnosis of tuberculous peritonitis,” Gut, vol. 27, pp. 1049-1053, 1986.
[19] A. Riquelme, M. Calvo, F. Salech, S. Valderrama, A. Pattillo, M. Arellano, M. Arrese, A. Soza, P. Viviani and L.M. Letelier, “Value of Adenosine Deaminase (ADA) in Ascitic Fluid for the Diagnosis of Tuberculous Peritonitis,” J Clin Gastroenterol, vol. 40, pp. 705-710, 2006.
[20] G. Ellis, D.M. Goldberg, “A reduced nicotinamide adenine dinucleotide--linked kinetic assay for adenosine deaminase activity,” J Lab Clin Med, vol. 76, pp. 507-17, 1970.
[21] K. Shadia, S. Anwar, S. Banu, A. A. Saleh and M. R. A. Miah, “Comparison of three mycobacterial DNA extraction methods from extrapulmonary samples for PCR assay,” Ibrahim Med. Coll. J, vol 6(1), pp. 9-11, 2012.
[22] M. Hajia, M. Rahbar, R. Amini, “Is PCR assay reliable for diagnosis of extrapulmonary tuberculosis?,” African J Microbiol Res, vol. 3(12), pp. 877-881, 2009.
[23] S. Das, C.N. Paramasivan, D.B. Lewis, R. Prabhakar, P.R. Narayanan, “IS6110 restriction fragment length polymorphism typing of clinical isolates of Mycobacterium tuberculosis from patients with pulmonary tuberculosis in Madras, south India,” Tubercle Lung Dis, vol. 76, pp. 550-4, 1995.
[24] N.G. Fumokong, T.H. Tang S. Al-Maamary, “Insertion sequence typing of M. tuberculosis: Characterization of a widespread sub-type with single copy of IS6110,” Tubercle Lung Dis, vol. 75, pp. 435–440, 1994.
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  • APA Style

    Khandaker Shadia, S. M. Mostofa Kamal, Ahmed Abu Saleh, Mohammed Nayem Hossain, Ratan Das Gupta, et al. (2015). Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. American Journal of Biomedical and Life Sciences, 3(3), 49-54. https://doi.org/10.11648/j.ajbls.20150303.14

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    ACS Style

    Khandaker Shadia; S. M. Mostofa Kamal; Ahmed Abu Saleh; Mohammed Nayem Hossain; Ratan Das Gupta, et al. Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. Am. J. Biomed. Life Sci. 2015, 3(3), 49-54. doi: 10.11648/j.ajbls.20150303.14

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    AMA Style

    Khandaker Shadia, S. M. Mostofa Kamal, Ahmed Abu Saleh, Mohammed Nayem Hossain, Ratan Das Gupta, et al. Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. Am J Biomed Life Sci. 2015;3(3):49-54. doi: 10.11648/j.ajbls.20150303.14

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  • @article{10.11648/j.ajbls.20150303.14,
      author = {Khandaker Shadia and S. M. Mostofa Kamal and Ahmed Abu Saleh and Mohammed Nayem Hossain and Ratan Das Gupta and Md. Ruhul Amin Miah},
      title = {Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {3},
      number = {3},
      pages = {49-54},
      doi = {10.11648/j.ajbls.20150303.14},
      url = {https://doi.org/10.11648/j.ajbls.20150303.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150303.14},
      abstract = {Diagnosis of tuberculosis from different body fluids remains challenging due to various limitations of the conventional and molecular methods. We studied the role of adenosine deaminase (ADA) assay to diagnose tubercular infection in cerebrospinal fluid, peritoneal fluid and pleural fluid. Fifty three patients with tubercular meningitis, peritonitis and pleuritis were enrolled in this study on the basis of clinical, radiological, cytological, biochemical and somewhere bacteriological evidences. Cases positive by AFB smear, culture or PCR were considered as confirmed TB and other as probable TB cases. Another 28 non-TB cases were included as control. In 53 suspected TB cases ADA was found positive in highest 42 (79.2%) cases, whereas smear and/ culture in 10 (18.7%) and PCR in 18 (33.9%) cases. ADA assay revealed 100% positivity in confirmed TB cases and 14.3% in non TB cases. The sensitivity and specificity of ADA was found 79% and 86% respectively when the cut off value was used ≥ 10 IU/L for CSF and ≥ 40 IU/L for pleural or peritoneal fluid. Mean ADA values of confirmed and probable TB cases were found significantly higher than that of non TB cases (< 0.05). Significance of difference was determined by ANOVA and Kruskal-Wallis test. Thus, adenosine deaminase assay in body fluids has proved to be efficient, reliable and simple method to diagnose tubercular meningitis, peritonitis and pleuritis.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection
    AU  - Khandaker Shadia
    AU  - S. M. Mostofa Kamal
    AU  - Ahmed Abu Saleh
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    AU  - Ratan Das Gupta
    AU  - Md. Ruhul Amin Miah
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    N1  - https://doi.org/10.11648/j.ajbls.20150303.14
    DO  - 10.11648/j.ajbls.20150303.14
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 49
    EP  - 54
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20150303.14
    AB  - Diagnosis of tuberculosis from different body fluids remains challenging due to various limitations of the conventional and molecular methods. We studied the role of adenosine deaminase (ADA) assay to diagnose tubercular infection in cerebrospinal fluid, peritoneal fluid and pleural fluid. Fifty three patients with tubercular meningitis, peritonitis and pleuritis were enrolled in this study on the basis of clinical, radiological, cytological, biochemical and somewhere bacteriological evidences. Cases positive by AFB smear, culture or PCR were considered as confirmed TB and other as probable TB cases. Another 28 non-TB cases were included as control. In 53 suspected TB cases ADA was found positive in highest 42 (79.2%) cases, whereas smear and/ culture in 10 (18.7%) and PCR in 18 (33.9%) cases. ADA assay revealed 100% positivity in confirmed TB cases and 14.3% in non TB cases. The sensitivity and specificity of ADA was found 79% and 86% respectively when the cut off value was used ≥ 10 IU/L for CSF and ≥ 40 IU/L for pleural or peritoneal fluid. Mean ADA values of confirmed and probable TB cases were found significantly higher than that of non TB cases (< 0.05). Significance of difference was determined by ANOVA and Kruskal-Wallis test. Thus, adenosine deaminase assay in body fluids has proved to be efficient, reliable and simple method to diagnose tubercular meningitis, peritonitis and pleuritis.
    VL  - 3
    IS  - 3
    ER  - 

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Author Information
  • Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

  • National Tuberculosis Reference Laboratory, Institute of Diseases of the Chest and Hospital (NIDCH), Dhaka, Bangladesh

  • Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

  • Department of Surgery, Dhaka Medical College Hospital, Dhaka, Bangladesh

  • Department of Medicine, Dhaka Medical College Hospital, Dhaka, Bangladesh

  • Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

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