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Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model

Received: 11 December 2014     Accepted: 22 December 2014     Published: 8 January 2015
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Abstract

Rotator cuff tears are a common injury in the elderly and often impose limitations on the range of motion and cause pain. The cause of rotator cuff tearing is unknown, and degeneration of the supraspinatus tendon with age is poorly understood.In the present study, we examined collagen degeneration of the supraspinatus tendon in the rat shoulder joint. Using rats of different age (10 weeks: young, 6 months: adult, and 2 years: old), we examined the supraspinatus tendon by immunofluorescence staining (collagen types I and III), assessed the amount of collagen and mRNA expression for collagen type 1A1, 3A1 (COL1A1, COL3A1), matrix metalloproteinase 2,9 (MMP-2, -9).After immunofluorescence staining, the layer structure of tendon to bone attachment was apparent in the young. The amount of collagen decreased in the old group. In addition the mRNA expression of Col1a1 and Col3a1 was significantly reduced in the old group.On the one hand, the old groups showed higher mRNA levels of MMP-2 and MMP-9 than the young group. The ability to synthesize collagen decreases during aging and the amount of total collagen that is present in the supraspinatus tendon is decreased. These changes may contribute to tearing/breakage of the supraspinatus tendon.

Published in American Journal of Biomedical and Life Sciences (Volume 2, Issue 6)
DOI 10.11648/j.ajbls.20140206.14
Page(s) 156-162
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Supraspinatus Tendon, Aging, Collagen

References
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  • APA Style

    Kenji Murata, Naohiko Kanemura, Takanori Kokubun, Tsutomu Fujino, Junichi Matsumoto, et al. (2015). Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model. American Journal of Biomedical and Life Sciences, 2(6), 156-162. https://doi.org/10.11648/j.ajbls.20140206.14

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    ACS Style

    Kenji Murata; Naohiko Kanemura; Takanori Kokubun; Tsutomu Fujino; Junichi Matsumoto, et al. Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model. Am. J. Biomed. Life Sci. 2015, 2(6), 156-162. doi: 10.11648/j.ajbls.20140206.14

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    AMA Style

    Kenji Murata, Naohiko Kanemura, Takanori Kokubun, Tsutomu Fujino, Junichi Matsumoto, et al. Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model. Am J Biomed Life Sci. 2015;2(6):156-162. doi: 10.11648/j.ajbls.20140206.14

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  • @article{10.11648/j.ajbls.20140206.14,
      author = {Kenji Murata and Naohiko Kanemura and Takanori Kokubun and Tsutomu Fujino and Junichi Matsumoto and Kenji Yasui and Kiyomi Takayanagi},
      title = {Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {2},
      number = {6},
      pages = {156-162},
      doi = {10.11648/j.ajbls.20140206.14},
      url = {https://doi.org/10.11648/j.ajbls.20140206.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140206.14},
      abstract = {Rotator cuff tears are a common injury in the elderly and often impose limitations on the range of motion and cause pain. The cause of rotator cuff tearing is unknown, and degeneration of the supraspinatus tendon with age is poorly understood.In the present study, we examined collagen degeneration of the supraspinatus tendon in the rat shoulder joint. Using rats of different age (10 weeks: young, 6 months: adult, and 2 years: old), we examined the supraspinatus tendon by immunofluorescence staining (collagen types I and III), assessed the amount of collagen and mRNA expression for collagen type 1A1, 3A1 (COL1A1, COL3A1), matrix metalloproteinase 2,9 (MMP-2, -9).After immunofluorescence staining, the layer structure of tendon to bone attachment was apparent in the young. The amount of collagen decreased in the old group. In addition the mRNA expression of Col1a1 and Col3a1 was significantly reduced in the old group.On the one hand, the old groups showed higher mRNA levels of MMP-2 and MMP-9 than the young group. The ability to synthesize collagen decreases during aging and the amount of total collagen that is present in the supraspinatus tendon is decreased. These changes may contribute to tearing/breakage of the supraspinatus tendon.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model
    AU  - Kenji Murata
    AU  - Naohiko Kanemura
    AU  - Takanori Kokubun
    AU  - Tsutomu Fujino
    AU  - Junichi Matsumoto
    AU  - Kenji Yasui
    AU  - Kiyomi Takayanagi
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    DO  - 10.11648/j.ajbls.20140206.14
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 156
    EP  - 162
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20140206.14
    AB  - Rotator cuff tears are a common injury in the elderly and often impose limitations on the range of motion and cause pain. The cause of rotator cuff tearing is unknown, and degeneration of the supraspinatus tendon with age is poorly understood.In the present study, we examined collagen degeneration of the supraspinatus tendon in the rat shoulder joint. Using rats of different age (10 weeks: young, 6 months: adult, and 2 years: old), we examined the supraspinatus tendon by immunofluorescence staining (collagen types I and III), assessed the amount of collagen and mRNA expression for collagen type 1A1, 3A1 (COL1A1, COL3A1), matrix metalloproteinase 2,9 (MMP-2, -9).After immunofluorescence staining, the layer structure of tendon to bone attachment was apparent in the young. The amount of collagen decreased in the old group. In addition the mRNA expression of Col1a1 and Col3a1 was significantly reduced in the old group.On the one hand, the old groups showed higher mRNA levels of MMP-2 and MMP-9 than the young group. The ability to synthesize collagen decreases during aging and the amount of total collagen that is present in the supraspinatus tendon is decreased. These changes may contribute to tearing/breakage of the supraspinatus tendon.
    VL  - 2
    IS  - 6
    ER  - 

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Author Information
  • Department of Physical therapy, School of health and Social Services, Saitama Prefectural University, Saitama, Japan

  • Department of Physical therapy, School of health and Social Services, Saitama Prefectural University, Saitama, Japan

  • Department of Physical therapy, School of health and Social Services, Saitama Prefectural University, Saitama, Japan

  • Department of Rehabilitation, Urawa Orthopedics Clinic, Saitama, Japan

  • Department of Rehabilitation, Kasukabe Chuo general Hospital, Saitama, Japan

  • Department of Orthopedics, Tokyo Women’s Medical University, Tokyo, Japan

  • Department of Physical therapy, School of health and Social Services, Saitama Prefectural University, Saitama, Japan

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