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Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent

Received: 6 October 2014     Accepted: 21 October 2014     Published: 30 October 2014
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Abstract

A protein fraction isolated from green algae Halimeda macrobola taken from the sea of Selayar and Kapoposang Island in South Sulawesi was tested for antioxidant and anticancer properties. The protein was isolated using buffer Tris (hydroxymethyl) amino methane. Initial purification of protein was conducted by using the fractionation method with ammonium sulphate, followed by dialysis process. Protein concentration was determined by Lowry method. Antioxidant assay was done by using DPPH method and anticancer activity test by Brine Shrimp Lethality Test (BSLT) method. Anticancer activity was further confirmed by antimitotic test using urchin zygote cells. The results showed that the protein concentration of the crude extract was 0.920 mg/mL. The highest concentration of protein fractions was indicated by the fraction 40-60%, with 1.015 mg/mL. The strong antioxidant activity was shown in the protein fraction of 0-20% saturation with IC50 values of 0.110 mg/mL.The highest activity in the anticancer tests was shown in fractions 0-20% saturation with LC50 values of 0.29 µg/mL and IC50 value of 53.80 µg/mL. The protein fraction 0-20% saturation had a potential to be developed as antioxidant and anticancer agent. These results demonstrate that inexpensive green algae Halimeda macrobola could be a new alternative to produce antioxidant and anticancer proteins.

Published in American Journal of Biomedical and Life Sciences (Volume 2, Issue 5)
DOI 10.11648/j.ajbls.20140205.15
Page(s) 134-140
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Green Algae, Protein Fraction, Toxicity, Antioxidant, Anticancer

References
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[2] R. Rachmat, 1999, Natural Products Utilization of Marine Algae for Drugs and Cosmetics, Paper presented at the VII Forum Communications Procedings Pra Kipnas I Physiology Association of Indonesia (IFI), Indonesian Institute of Sciences (LIPI), Serpong, 08 September 1999,
[3] World Health Organization, 2012, Cancer (online), http://www.who.int/mediacentre/factsheets/fs297/index.html, acces on October 05, 2012.
[4] I.C., Sheih, T.J., Fang Wu, T.K., Lin, 2010, Anticancer and antioxidant activities of the peptide fraction from algae protein waste. J Agric Food Chem. 58(2), pp.1202-1207.
[5] T. Sugahara, Y. Ohama, A. Fukuda, et al. 2001, The cytotoxic effect of Eucheuma serra agglutinin (ESA) on cancer cells and its application to molecular probe for drug delivery system using lipid vesicles, Cytotechnology. 36(1-3), pp. 93–99.
[6] S. Dali, H. Natsir, H. Usman, and A. Ahmad, 2011, Antibacterial Bioactivity Protein Fraction of Red Algae Gelidium amansii from Takalar Cikoang Aquatic, South Sulawesi, Pharmacy Magazine and Pharmacology, 15 (1): pp. 47-52.
[7] M. Hayes, (Ed). 2012, Marine Bioactive Compounds: Sources, Characterization and Applications. Springer-Science, New York, London.
[8] H.C., Schro¨der, H., Ushijima, A., Krasko, V., Gamulin, N.L., Thakur, B., Diehl-Seifert, I.M., Muller, and W.E.G., Muller, 2003, Emergence and Disappearance of an immune molecule, an antimicrobial lectin, in basal metazoan. A TACHYLECTIN-RELATED PROTEIN IN THE SPONGE SUBERITES DOMUNCULA. J. Biol. Chem., 278, pp.32810-32817.
[9] O. H., Lowry, N. J., Rosebrough, A. L., Farr, and R. J., Randall, 1951, Protein Measurement with The Folin Phenol Reagent, J. Biol. Chem., 193: pp.265-275.
[10] Juniarti, D. Osmeli, and Yuhernita, 2009, Content of Chemical Compounds, Toxicity Test (Brine Shrimp Lethality Test) and Antioxidants (1,1-diphenyl-2-picrilhydrazil) from the Leaf Extract Saga (Abrus precatorius L.), Makara Sains, 13 (1): pp. 50-55.
[11] P. Molyneux, 2004, The use of stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, Songklanakarin J Sci Technol., 26 (2): pp. 211-219.
[12] N., Weerapreyakul, A., Nonpunya, S., Barusrux, T. Thitimetharoc, and B. Sripanidkulchai, 2012, Evaluation of the Anticancer Potential of Six Herbs against a Hepatoma Cell Line, Chinese Medicine, 7 (15): pp. 2-7.
[13] A. P. D., Nurhayati, N. Abdulgani, and R., Febrianto, 2006, Toxicity Test of Eucheuma alvarezii Extract against Artemia salina as Potential Anticancer Preliminary Study, Indonesia Chemical Deed (Kimindo Deed), 2 (1): pp.41-46.
[14] S. D., Marliyana, F. W., Rakhman, H., Nestri, and R., Rakhmawati, 2012, Toxicity Test (Brine Shrimp Lethality Test) of Whole Extract from Pandanus conoideus L., Alchemy Jurnal Penelitian Kimia, 8 (1): pp. 24-33.
[15] B. N., Meyer, N. R., Ferrigni, J. E., Putman, L. B., Jacbsen, D. E., Nicols, and J. L. McLaughlin, 1982, Brine Shrimp : A Comvenient general Bioassay For Active Plant Constituents. Plant Medica. Pp.31-34.
[16] E., Johannes, Syafaraenan, R., Agus, dan R., Umar, 2013, Antimitotic activity of β-sitosterol Isolates from Hydroid Aglaophenia Cupressina Lamoureoux the Early Cleavage Cell Zygote of Urchin Tripneus tesgratilla Linn., MANASIR, 1 (1): pp. 27-32.
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    Ahyar Ahmad, Hanapi Usman, Hasnah Natsir, Abdul Karim. (2014). Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent. American Journal of Biomedical and Life Sciences, 2(5), 134-140. https://doi.org/10.11648/j.ajbls.20140205.15

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    ACS Style

    Ahyar Ahmad; Hanapi Usman; Hasnah Natsir; Abdul Karim. Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent. Am. J. Biomed. Life Sci. 2014, 2(5), 134-140. doi: 10.11648/j.ajbls.20140205.15

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    AMA Style

    Ahyar Ahmad, Hanapi Usman, Hasnah Natsir, Abdul Karim. Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent. Am J Biomed Life Sci. 2014;2(5):134-140. doi: 10.11648/j.ajbls.20140205.15

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  • @article{10.11648/j.ajbls.20140205.15,
      author = {Ahyar Ahmad and Hanapi Usman and Hasnah Natsir and Abdul Karim},
      title = {Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {2},
      number = {5},
      pages = {134-140},
      doi = {10.11648/j.ajbls.20140205.15},
      url = {https://doi.org/10.11648/j.ajbls.20140205.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140205.15},
      abstract = {A protein fraction isolated from green algae Halimeda macrobola taken from the sea of Selayar and Kapoposang Island in South Sulawesi was tested for antioxidant and anticancer properties. The protein was isolated using buffer Tris (hydroxymethyl) amino methane. Initial purification of protein was conducted by using the fractionation method with ammonium sulphate, followed by dialysis process. Protein concentration was determined by Lowry method. Antioxidant assay was done by using DPPH method and anticancer activity test by Brine Shrimp Lethality Test (BSLT) method. Anticancer activity was further confirmed by antimitotic test using urchin zygote cells. The results showed that the protein concentration of the crude extract was 0.920 mg/mL. The highest concentration of protein fractions was indicated by the fraction 40-60%, with 1.015 mg/mL.  The strong antioxidant activity was  shown in the protein fraction of 0-20% saturation with IC50 values of 0.110 mg/mL.The highest activity in the anticancer tests was shown in fractions 0-20% saturation with LC50 values of 0.29 µg/mL and IC50 value of 53.80 µg/mL. The protein fraction 0-20% saturation had a potential to be developed as antioxidant and anticancer agent. These results demonstrate that inexpensive green algae Halimeda macrobola could be a new alternative to produce antioxidant and anticancer proteins.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent
    AU  - Ahyar Ahmad
    AU  - Hanapi Usman
    AU  - Hasnah Natsir
    AU  - Abdul Karim
    Y1  - 2014/10/30
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajbls.20140205.15
    DO  - 10.11648/j.ajbls.20140205.15
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 134
    EP  - 140
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20140205.15
    AB  - A protein fraction isolated from green algae Halimeda macrobola taken from the sea of Selayar and Kapoposang Island in South Sulawesi was tested for antioxidant and anticancer properties. The protein was isolated using buffer Tris (hydroxymethyl) amino methane. Initial purification of protein was conducted by using the fractionation method with ammonium sulphate, followed by dialysis process. Protein concentration was determined by Lowry method. Antioxidant assay was done by using DPPH method and anticancer activity test by Brine Shrimp Lethality Test (BSLT) method. Anticancer activity was further confirmed by antimitotic test using urchin zygote cells. The results showed that the protein concentration of the crude extract was 0.920 mg/mL. The highest concentration of protein fractions was indicated by the fraction 40-60%, with 1.015 mg/mL.  The strong antioxidant activity was  shown in the protein fraction of 0-20% saturation with IC50 values of 0.110 mg/mL.The highest activity in the anticancer tests was shown in fractions 0-20% saturation with LC50 values of 0.29 µg/mL and IC50 value of 53.80 µg/mL. The protein fraction 0-20% saturation had a potential to be developed as antioxidant and anticancer agent. These results demonstrate that inexpensive green algae Halimeda macrobola could be a new alternative to produce antioxidant and anticancer proteins.
    VL  - 2
    IS  - 5
    ER  - 

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Author Information
  • Laboratory of Research Centre and Developing of Sciences, Mathematic and Natural Science Faculty, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia

  • Laboratory of Research Centre and Developing of Sciences, Mathematic and Natural Science Faculty, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia

  • Laboratory of Biochemistry, Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin Univesity, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia

  • Laboratory of Biochemistry, Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin Univesity, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia

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